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matrix hydrogel  (R&D Systems)


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    Structured Review

    R&D Systems matrix hydrogel
    Matrix Hydrogel, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bme/Cultrex+PathClear+BME%2C+Type+2+(w%2F+Phenol+Red)/pm39614182-41-9-20
    Average 94 stars, based on 26 article reviews
    matrix hydrogel - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium.
    Article Snippet: cDNA was amplified using primers from IDT and PowerUp SYBR Green master mix (Thermo Fisher) as per manufactures instructions.

    Article Title: Neuroimmune Communications Modulate Mucosal Immune Responses
    Article Snippet: Mouse colon was excised, cut along the mesenteric border, and then mounted onto cassettes (Physiologic Instruments) allowing for 0.1 cm2 of the mounted colonic tissue to be exposed to 4 mL of circulating oxygenated Ringer’s buffer (115 mM NaCl, 1.25 mM CaCl2, 1.2 mM MgCl2, 2.0 mM KH2PO4, and 25 mM NaHCO3) at 37°C.

    Injection:

    Article Title: Fine-Tuning Lipid-Coated Porous Silicon Nanoparticles for siRNA Delivery.
    Article Snippet: Small interfering RNA (siRNA) is a growing treatment avenue for cancer patients.. However, formulating siRNA to achieve high tumoral delivery efficiency is challenging.. Among various formulation strategies, porous silicon nanoparticles (pSiNPs) are unique due to the versatile nanoengineering options.

    Article Title: Small-molecule modulators of B56-PP2A restore 4E-BP function to suppress eIF4E-dependent translation in cancer cells
    Article Snippet: cDNA was generated from bead-bound RNA using the iScript cDNA Synthesis Kit and analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad). .. For SW-620 xenografts, nude mice were injected subcutaneously with 5 × 10 6 to 6 × 10 6 cells in sterile 50% PBS/50% Matrigel or Cultrex Type 2 (R&D Systems). ..

    Irradiation:

    Article Title: Polyclonal and single clonal patient-derived organoid models of Barrett oesophagus and oesophageal adenocarcinoma establish a platform for the analysis of heterogeneity in disease progression and therapy response
    Article Snippet: .. Given the known challenges of inter-batch heterogeneity in Cultrex BME Type 2 (R&D Systems), all biological replicates for irradiation experiments were performed using the same batch of BME Type 2. .. Integral area under the curve (AUC) values for organoid forming efficiency following irradiation were calculated using GraphPad Prism Version 10.4.1. for Windows (GraphPad Software, Boston, USA).

    Sterility:

    Article Title: Small-molecule modulators of B56-PP2A restore 4E-BP function to suppress eIF4E-dependent translation in cancer cells
    Article Snippet: cDNA was generated from bead-bound RNA using the iScript cDNA Synthesis Kit and analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad). .. For SW-620 xenografts, nude mice were injected subcutaneously with 5 × 10 6 to 6 × 10 6 cells in sterile 50% PBS/50% Matrigel or Cultrex Type 2 (R&D Systems). ..

    Transferring:

    Article Title: Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium
    Article Snippet: cDNA was amplified using primers from IDT and PowerUp SYBR Green master mix (Thermo Fisher) as per manufactures instructions. .. Tissue was minced until able to be passed through a 1 mL pipette tip then incubated in 2 mg/mL collagenase at 37 o C for 40 min. Crypt units were dissociated by vigorous pipetting then washed 3x in PBS containing 10% FBS and plated into 40 μL Matrigel domes (Cultrex PathClear reduced growth factor BME, type 2; R&D Systems) in a 24 well plate. .. pcDNA3.1 vectors expressing human caspase-4, human IL-18 and human GSDMD were purchased from Genscript.

    Incubation:

    Article Title: Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium
    Article Snippet: cDNA was amplified using primers from IDT and PowerUp SYBR Green master mix (Thermo Fisher) as per manufactures instructions. .. Tissue was minced until able to be passed through a 1 mL pipette tip then incubated in 2 mg/mL collagenase at 37 o C for 40 min. Crypt units were dissociated by vigorous pipetting then washed 3x in PBS containing 10% FBS and plated into 40 μL Matrigel domes (Cultrex PathClear reduced growth factor BME, type 2; R&D Systems) in a 24 well plate. .. pcDNA3.1 vectors expressing human caspase-4, human IL-18 and human GSDMD were purchased from Genscript.

    Article Title: Host age at experimental Helicobacter pylori infection shapes epithelial development of mouse gastric organoids
    Article Snippet: .. For seeding, glands were resuspended in Cultrex type 2 (R&D Systems) on ice and 50 μl was placed in the center of each well of a pre-warmed 24-well plate creating a dome Following a brief incubation, domes were overlaid with 500 μL of gastric organoid medium (basal medium supplemented with 3dGRO L-WRN conditioned media, Sigma-Aldrich), gastrin (Phoenix Pharmaceuticals), EGF and FGF-10 (R&D Systems), N-acetylcysteine (Sigma-Aldrich/Merck), B27 (Invitrogen), and the ROCK inhibitor (Invitrogen). .. Organoids were cultured at 37 °C in 7% O2 and 5% CO2 (HERACELL VIOS 160i, Thermo Fisher Scientific).



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