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Clinical and Laboratory Standards Institute reference broth microdilution (bmd) method
Reference Broth Microdilution (Bmd) Method, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bmd+reference+method/broth+microdilution++bmd++method/pm37966262-60-13-20
Average 90 stars, based on 1 article reviews
reference broth microdilution (bmd) method - by Bioz Stars, 2026-09
90/100 stars

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Article Title: An AIE-active probe acts as novel tool for rapid and accurate quantitative antifungal susceptibility assessment
Article Snippet: The Clinical and Laboratory Standards Institute [CLSI; formerly the National Committee for Clinical Laboratory Standards (NCCLS)] has developed and published an approved reference method for broth microdilution testing (BMD, CLSI document M27-A4) of Candida species ( ).

Article Title: Comparative evaluation of antifungal susceptibility testing methods of invasive Candida species and detection of FKS genes mutations in caspofungin intermediate and resistant isolates.
Article Snippet: Broth microdilution (BMD) is the preferred test for antifungal susceptibility in Candida spp. by determining the minimum inhibitory concentration (MIC) following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute (CLSI) reference standards [4, 5].

Article Title: Mycobacteroides abscessus subspecies: a comparative genomic analysis reveals unique metabolic activities and drug resistance patterns
Article Snippet: Following the previously established research methodology [ , ], we measured the MIC values of clinical and standard strains using the broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute (CLSI) guidelines ( https://www.ncbi.nlm.nih.gov/books/NBK544374/table/tab_7/?report=objectonly ).

Article Title: Prevalence of colistin-resistant Enterobacteriaceae isolated from clinical samples in Africa: a systematic review and meta-analysis
Article Snippet: As recommended jointly by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Test (EUCAST), broth microdilution (BMD) method is now the only gold standard for determining colistin minimum inhibition concentration (MIC) values [ ].

Article Title: Prevalence of colistin-resistant Enterobacteriaceae isolated from clinical samples in Africa: a systematic review and meta-analysis.
Article Snippet: As recommended jointly by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Test (EUCAST), broth microdilution (BMD) method is now the only gold standard for determining colistin minimum inhibition concentration (MIC) values [19].

Article Title: Comparative Analysis of Antifungal Susceptibility in Trichophyton Species Using Broth Microdilution Assay: A Cross-Sectional Study
Article Snippet: The broth microdilution (BMD) assay, endorsed by the Clinical and Laboratory Standards Institute (CLSI), is a gold standard method for determining minimum inhibitory concentrations (MICs) of antifungal drugs [ ].

Article Title: Mycobacteroides abscessus subspecies: a comparative genomic analysis reveals unique metabolic activities and drug resistance patterns.
Article Snippet: Following the previously established research methodology [32, 33], we measured the MIC values of clinical and standard strains using the broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute (CLSI) guidelines ( h t t p s : / / w w w . n c b i . n l m . n i h . g o v / b o o k s / N B K 5 4 4 3 7 4 / t a b l e / t a b _ 7 / ? r e p o r t = o b j e c t o n l y).

Diffusion-based Assay:

Article Title: In-vitro susceptibility of cefiderocol and other comparators in carbapenem-resistant Gram-negative bacilli: A study from India.
Article Snippet: Introduction: Cefiderocol is a parenteral catechol-type siderophore cephalosporin, which has been approved for the treatment of Gram-negative bacterial infections.. Its activity among the carbapenem-resistant Gram-negative bacilli (CR-GNBs) in India is largely unknown.. Methodology: We tested in-vitro susceptibility of cefiderocol in 84 CR-GNB [ carbapenem-resistant Acinetobacter baumannii (CRAB), carbapenem-resistant Pseudomonas aeruginosa (CRPA), carbapenem-resistant Escherichia coli (CREC) and carbapenem-resistant Klebsiella pneumoniae (CRKP)] by broth microdilution (BMD) and disc diffusion (DD) using Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints and concordance of DD was compared with BMD.



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Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and <t>broth</t> <t>microdilution</t> <t>(BMD)</t> to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.
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Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and <t>broth</t> <t>microdilution</t> <t>(BMD)</t> to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.
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Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and <t>broth</t> <t>microdilution</t> <t>(BMD)</t> to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.
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Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and <t>broth</t> <t>microdilution</t> <t>(BMD)</t> to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.
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Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and broth microdilution (BMD) to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.

Journal: Frontiers in Microbiology

Article Title: SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples

doi: 10.3389/fmicb.2018.02809

Figure Lengend Snippet: Study procedure. Minimum inhibitory concentrations (MIC) of all colistin non-intrinsically resistant bacterial species growing on SuperPolymyxin™ medium were tested by gradient diffusion test (Etest®, bioMérieux) and broth microdilution (BMD) to confirm colistin resistance. Presence of the transferable resistance determinant mcr-1 was tested by PCR.

Article Snippet: Colistin resistance was confirmed by the BMD reference method (Sensititre TM FRCOL, Thermo Fisher Scientific, Wesel, Germany) according to the manufacturer's instruction.

Techniques: Diffusion-based Assay

Comparison of Etest® and  broth microdilution  for  colistin  susceptibility testing.

Journal: Frontiers in Microbiology

Article Title: SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples

doi: 10.3389/fmicb.2018.02809

Figure Lengend Snippet: Comparison of Etest® and broth microdilution for colistin susceptibility testing.

Article Snippet: Colistin resistance was confirmed by the BMD reference method (Sensititre TM FRCOL, Thermo Fisher Scientific, Wesel, Germany) according to the manufacturer's instruction.

Techniques: Comparison

Comparison of the Rapid Polymyxin™ NP Test and  broth microdilution  to detect  colistin  resistance.

Journal: Frontiers in Microbiology

Article Title: SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples

doi: 10.3389/fmicb.2018.02809

Figure Lengend Snippet: Comparison of the Rapid Polymyxin™ NP Test and broth microdilution to detect colistin resistance.

Article Snippet: Colistin resistance was confirmed by the BMD reference method (Sensititre TM FRCOL, Thermo Fisher Scientific, Wesel, Germany) according to the manufacturer's instruction.

Techniques: Comparison