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axio observer 7  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio observer 7
    Axio Observer 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+microscope/Inverted+microscope+Axio+Observer+7/pmc12951291-10-7-80
    Average 98 stars, based on 985 article reviews
    axio observer 7 - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Bidirectional coupling of neuronal Ca 2+ and nitric oxide signals visualized by a dual biosensor
    Article Snippet: .. Live-cell widefield epifluorescence imaging was performed using a Zeiss Axio Observer Z1.7 inverted microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with Plan-Apochromat 20 × /0.8 dry objective and an Axiocam 503 monochrome CCD camera. ..

    Article Title: 5′untranslated regions provide a versatile toolkit for tunable exogenous protein expression
    Article Snippet: After adhering to the glass slide for 1 h, cells were loaded with 10 μg/ml Fura 2-AM (ION Biosciences) in physiological Ringer's solution (in mM: 120 NaCl, 5 KCl, 2 CaCl 2 , 25 NaHCO 3 , 1 MgCl 2 , 5.5 HEPES, 1 D-glucose, pH 7.4; Boston BioProducts) with 0.025% Pluronic F-127 (Sigma) and 1% DMSO, and incubated for 45 min at 37°C, then rinsed twice with Ringer's solution. .. Ratiometric Ca 2+ imaging was performed using a Zeiss Axio Observer 7 inverted microscope with a Hamamatsu Flash sCMOS camera at a 20× objective. ..

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Article Title: Colloid-patterned surfaces distinguish malignant mechanophenotypes
    Article Snippet: The system was integrated with 405, 488, 561, and 638 nm LH + lasers and a CFI Plan Apochromat Lambda D 100 × oil immersion objective (numerical aperture 1.45, working distance 0.13 mm; Nikon Corporation, Tokyo, Japan). .. Imaging of surfaces coated with fluorescent colloids and cultured cells was also performed using a Zeiss LSM 980 confocal microscope with Airyscan detection (Carl Zeiss Microscopy GmbH, Oberkochen, Germany), mounted on a Zeiss Axio Observer 7 SP inverted platform and equipped with a Plan-Apochromat 63 × /1.4 oil DIC objective. .. Image acquisition was controlled using ZEN software (Carl Zeiss Microscopy GmbH).

    Inverted Microscopy:

    Article Title: Bidirectional coupling of neuronal Ca 2+ and nitric oxide signals visualized by a dual biosensor
    Article Snippet: .. Live-cell widefield epifluorescence imaging was performed using a Zeiss Axio Observer Z1.7 inverted microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with Plan-Apochromat 20 × /0.8 dry objective and an Axiocam 503 monochrome CCD camera. ..

    Article Title: 5′untranslated regions provide a versatile toolkit for tunable exogenous protein expression
    Article Snippet: After adhering to the glass slide for 1 h, cells were loaded with 10 μg/ml Fura 2-AM (ION Biosciences) in physiological Ringer's solution (in mM: 120 NaCl, 5 KCl, 2 CaCl 2 , 25 NaHCO 3 , 1 MgCl 2 , 5.5 HEPES, 1 D-glucose, pH 7.4; Boston BioProducts) with 0.025% Pluronic F-127 (Sigma) and 1% DMSO, and incubated for 45 min at 37°C, then rinsed twice with Ringer's solution. .. Ratiometric Ca 2+ imaging was performed using a Zeiss Axio Observer 7 inverted microscope with a Hamamatsu Flash sCMOS camera at a 20× objective. ..

    Article Title: Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy
    Article Snippet: .. The equipment and settings for the presented protocol used were as follows: a. Zeiss Axio Observer.Z1/7 inverted microscope with LSM 980 confocal scanner. b. ..

    Microscopy:

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Article Title: Colloid-patterned surfaces distinguish malignant mechanophenotypes
    Article Snippet: The system was integrated with 405, 488, 561, and 638 nm LH + lasers and a CFI Plan Apochromat Lambda D 100 × oil immersion objective (numerical aperture 1.45, working distance 0.13 mm; Nikon Corporation, Tokyo, Japan). .. Imaging of surfaces coated with fluorescent colloids and cultured cells was also performed using a Zeiss LSM 980 confocal microscope with Airyscan detection (Carl Zeiss Microscopy GmbH, Oberkochen, Germany), mounted on a Zeiss Axio Observer 7 SP inverted platform and equipped with a Plan-Apochromat 63 × /1.4 oil DIC objective. .. Image acquisition was controlled using ZEN software (Carl Zeiss Microscopy GmbH).

    Article Title: Volume and surface methods for microparticle traction force microscopy: a computational and experimental comparison
    Article Snippet: .. Microwells for DNA microparticle trapping were fabricated using a custom-engineered setup, consisting of a Polygon digital micromirror device (DMD) pattern illuminator (MIGHTEX Polygon 1000) coupled to an inverted fluorescence microscope (Axio Observer 7, Carl Zeiss AG). ..

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions
    Article Snippet: .. Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7). ..

    Fluorescence:

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Article Title: Volume and surface methods for microparticle traction force microscopy: a computational and experimental comparison
    Article Snippet: .. Microwells for DNA microparticle trapping were fabricated using a custom-engineered setup, consisting of a Polygon digital micromirror device (DMD) pattern illuminator (MIGHTEX Polygon 1000) coupled to an inverted fluorescence microscope (Axio Observer 7, Carl Zeiss AG). ..

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions
    Article Snippet: .. Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7). ..

    Cell Culture:

    Article Title: Colloid-patterned surfaces distinguish malignant mechanophenotypes
    Article Snippet: The system was integrated with 405, 488, 561, and 638 nm LH + lasers and a CFI Plan Apochromat Lambda D 100 × oil immersion objective (numerical aperture 1.45, working distance 0.13 mm; Nikon Corporation, Tokyo, Japan). .. Imaging of surfaces coated with fluorescent colloids and cultured cells was also performed using a Zeiss LSM 980 confocal microscope with Airyscan detection (Carl Zeiss Microscopy GmbH, Oberkochen, Germany), mounted on a Zeiss Axio Observer 7 SP inverted platform and equipped with a Plan-Apochromat 63 × /1.4 oil DIC objective. .. Image acquisition was controlled using ZEN software (Carl Zeiss Microscopy GmbH).

    Staining:

    Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
    Article Snippet: .. Finally, the cells were stained with the Calcein-AM/PI assay kit (Beyotime, Shanghai, China) and observed under an inverted fluorescence microscope (Zeiss Axio Observer7, Germany). ..

    Incubation:

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions
    Article Snippet: .. Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7). ..



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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture