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igg2b isotype control antibodies  (R&D Systems)


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    Structured Review

    R&D Systems igg2b isotype control antibodies
    Igg2b Isotype Control Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/IC013P/Rat+IgG2B+PE-conjugated+Antibody/us11434289-625-3-7
    Average 94 stars, based on 14 article reviews
    igg2b isotype control antibodies - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Control:

    Article Title: Alzheimer's disease-associated R47H TREM2 increases, but wild-type TREM2 decreases, microglial phagocytosis of synaptosomes and neuronal loss.
    Article Snippet: Funding information Innovative Medicines Initiative, Grant/Award Number: 115976; European Union's Horizon 2020 Research and Innovation Programme; EFPIA; Biotechnology and Biological Sciences Research Council, Grant/Award Number: BB/ R506047/1 Abstract Triggering receptor on myeloid cells 2 (TREM2) is an innate immune receptor, upregulated on the surface of microglia associated with amyloid plaques in Alzheimer's disease (AD).. Individuals heterozygous for the R47H variant of TREM2 have greatly increased risk of developing AD.. We examined the effects of wild-type (WT), R47H and knock-out (KO) of human TREM2 expression in three microglial cell systems.

    Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
    Article Snippet: .. Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. ..

    Article Title: B Cell Chronic Lymphocytic Leukemia Development in Mice with Chronic Lung Exposure to Coccidioides Fungal Arthroconidia
    Article Snippet: Fluorochrome-conjugated Abs used for these determinations were specific for the following: B220-AF488 (BD Pharmingen, 557669), CD5-PE (BD Pharmingen 553023, clone 53-7.3), CD19-allophycocyanin (BioLegend, clone 1B3), CD45-PerCP-Cy5.5 (BD Biosciences, 550994), CD11b-allophycocyanin (clone M1/70, BioLegend), Ly6G-allophycocyanin-eBio780 (eBioscience, clone 1AB-Ly6G; Invitrogen, 47-9668-80), CD115-PE (BioLegend, 135506, clone AFS98), and Ly6C-AF488 (BioLegend, 128021, clone HK1.4). .. Control IgG Abs included the following: FITC-mouse IgG2a (BD Biosciences, clone G20-127), PE–rat IgG2b (R&D Systems, IC013P), PerCP mouse IgG1 (BD Biosciences, 349044), and allophycocyanin–mouse IgG1 (R&D Systems, 1C002A). ..

    Article Title: B Cell Chronic Lymphocytic Leukemia Development in Mice with Chronic Lung Exposure to Coccidioides Fungal Arthroconidia.
    Article Snippet: Fluorochrome-conjugated Abs used for these determinations were specific for the following: B220-AF488 (BD Pharmingen, 557669), CD5-PE (BD Pharmingen 553023, clone 53-7.3), CD19allophycocyanin (BioLegend, clone 1B3), CD45-PerCP-Cy5.5 (BD Biosciences, 550994), CD11b-allophycocyanin (clone M1/70, BioLegend), Ly6G-allophycocyanin-eBio780 (eBioscience, clone 1AB-Ly6G; Invitrogen, 47-9668-80), CD115-PE (BioLegend, 135506, clone AFS98), and Ly6C-AF488 (BioLegend, 128021, clone HK1.4). .. Control IgG Abs included the following: FITC-mouse IgG2a (BD Biosciences, clone G20-127), PE rat IgG2b (R&D Systems, IC013P), PerCP mouse IgG1 (BD Biosciences, 349044), and allophycocyanin mouse IgG1 (R&D Systems, 1C002A). ..

    Article Title: Microglial signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
    Article Snippet: .. Myeloid progenitor cells were treated in suspension for 1 h with 5 μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920; R&D Systems) before being treated with 10 μg F(ab’)2 (Jackson #712006153) for 2 min of crosslinking. .. Cells were pelleted and lysed on ice in modified RIPA buffer (25 mM Tris pH7.4, 50 mM NaCl, 0.5% NP-40, 0.25% Na deoxycholate, 1 mM EDTA) containing 1 × Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) for subsequent western blot analysis.

    Article Title: Alzheimer's disease‐associated R47H TREM2 increases, but wild‐type TREM2 decreases, microglial phagocytosis of synaptosomes and neuronal loss
    Article Snippet: .. For detection of human TREM2 on CHME‐3 cells and BV‐2 cells, rat anti‐human/mouse TREM2 (clone: 237920) conjugated to Phycoerythrin (PE) fluorophore (R&D systems, FAB17291P) and isotype control, monoclonal rat IgG2B (clone: 141945) conjugated to PE fluorophore (R&D systems, IC013P) were used. .. Briefly, antibodies were diluted 1:50 (working concentration of 0.5 μg/ml) in ice‐cold flow cytometry staining buffer 1X (R&D Systems, FC001), added to tubes containing 100,000 cells/tube and kept in the dark at 4°C (to prevent receptor internalization) for 30 min.

    Negative Control:

    Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
    Article Snippet: .. Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. ..

    Concentration Assay:

    Article Title: Biomimetic Nanocarriers of Pro-Resolving Lipid Mediators for Resolution of Inflammation in Atherosclerosis.
    Article Snippet: The pellet obtained was washed and reconstituted in 100 μL of PBS containing 0.5% PFA, 5 mm EDTA, 5% FBS (FACS buffer), and the fluorochromecoupled antibody at a concentration of 5 μg mL−1: mouse anti-ChemR23 PE-conjugated Antibody (R&D Systems, cat. no. FAB7610P), rabbit antiGPR18 FITC-conjugated Antibody (Novus Biologicals, cat. no. NBP224918F), rabbit anti-FPR2 FITC-conjugated Antibody (Novus Biologicals, cat. no. NLS1878F), and rabbit anti-Lfr6 FITC-conjugated Antibody (Novus Biologicals, cat. no. FAB8458RF). .. Corresponding isotypes were used as controls: rat anti-IgG2B PE-conjugated Antibody (for ChemR23 receptor; R&D Systems, cat. no. IC013P), and rabbit anti-IgG FITC-conjugated Antibody (for GPR18 and FPR2 receptors; R&D Systems, cat. no. IC1051F) at a concentration of 10 μL/106 cells), as well as mouse anti-IgG F(ab) FITC-conjugated Antibody (for the Lgr6 receptor; Novus Biologicals, cat. no. NBP1-97143) at a concentration of 5 μg mL−1. ..

    Suspension:

    Article Title: Microglial signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
    Article Snippet: .. Myeloid progenitor cells were treated in suspension for 1 h with 5 μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920; R&D Systems) before being treated with 10 μg F(ab’)2 (Jackson #712006153) for 2 min of crosslinking. .. Cells were pelleted and lysed on ice in modified RIPA buffer (25 mM Tris pH7.4, 50 mM NaCl, 0.5% NP-40, 0.25% Na deoxycholate, 1 mM EDTA) containing 1 × Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) for subsequent western blot analysis.



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