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pe rat igg2b  (R&D Systems)


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    Structured Review

    R&D Systems pe rat igg2b
    Pe Rat Igg2b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/IC013P/Rat+IgG2B+PE-conjugated+Antibody/pmc11469162-340-9-12
    Average 92 stars, based on 32 article reviews
    pe rat igg2b - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Biomimetic Nanocarriers of Pro‐Resolving Lipid Mediators for Resolution of Inflammation in Atherosclerosis
    Article Snippet: The pellet obtained was washed and reconstituted in 100 μL of PBS containing 0.5% PFA, 5 m m EDTA, 5% FBS (FACS buffer), and the fluorochrome‐coupled antibody at a concentration of 5 μg mL −1 : mouse anti‐ChemR23 PE‐conjugated Antibody (R&D Systems, cat. no. FAB7610P), rabbit anti‐GPR18 FITC‐conjugated Antibody (Novus Biologicals, cat. no. NBP2‐24918F), rabbit anti‐FPR2 FITC‐conjugated Antibody (Novus Biologicals, cat. no. NLS1878F), and rabbit anti‐Lfr6 FITC‐conjugated Antibody (Novus Biologicals, cat. no. FAB8458RF). .. Corresponding isotypes were used as controls: rat anti‐IgG2B PE‐conjugated Antibody (for ChemR23 receptor; R&D Systems, cat. no. IC013P), and rabbit anti‐IgG FITC‐conjugated Antibody (for GPR18 and FPR2 receptors; R&D Systems, cat. no. IC1051F) at a concentration of 10 μL/10 6 cells), as well as mouse anti‐IgG F(ab) FITC‐conjugated Antibody (for the Lgr6 receptor; Novus Biologicals, cat. no. NBP1‐97143) at a concentration of 5 μg mL −1 . ..

    Virus:

    Article Title: Structure-Function Analysis of CCL28 in the Development of Post-viral Asthma
    Article Snippet: On day 49 PI, mice were euthanized, and one lung was removed for mRNA isolation with TRIzol (Sigma). cDNA was generated with the QuantiTect reverse transcription kit (Qiagen) as per the manufacturer's instructions, and qPCR was performed using TaqMan fast master mix and the StepOnePlus PCR system (Applied Biosystems) to compare CCL28 (assay ID Mm00445039_m1) to GAPDH (assay ID 4352339E) copy number. .. Anti-CCL28 Treatment of Sendai Virus-induced Airway Disease Mice were inoculated with SeV as above and injected subcutaneously with 200 μg of either anti-CCL28 (clone 134306; R&D Systems) or control rat IgG2b (clone 141945; R&D Systems) weekly from day 14 to 49 PI SeV. ..

    Mouse Assay:

    Article Title: Structure-Function Analysis of CCL28 in the Development of Post-viral Asthma
    Article Snippet: On day 49 PI, mice were euthanized, and one lung was removed for mRNA isolation with TRIzol (Sigma). cDNA was generated with the QuantiTect reverse transcription kit (Qiagen) as per the manufacturer's instructions, and qPCR was performed using TaqMan fast master mix and the StepOnePlus PCR system (Applied Biosystems) to compare CCL28 (assay ID Mm00445039_m1) to GAPDH (assay ID 4352339E) copy number. .. Anti-CCL28 Treatment of Sendai Virus-induced Airway Disease Mice were inoculated with SeV as above and injected subcutaneously with 200 μg of either anti-CCL28 (clone 134306; R&D Systems) or control rat IgG2b (clone 141945; R&D Systems) weekly from day 14 to 49 PI SeV. ..

    Injection:

    Article Title: Structure-Function Analysis of CCL28 in the Development of Post-viral Asthma
    Article Snippet: On day 49 PI, mice were euthanized, and one lung was removed for mRNA isolation with TRIzol (Sigma). cDNA was generated with the QuantiTect reverse transcription kit (Qiagen) as per the manufacturer's instructions, and qPCR was performed using TaqMan fast master mix and the StepOnePlus PCR system (Applied Biosystems) to compare CCL28 (assay ID Mm00445039_m1) to GAPDH (assay ID 4352339E) copy number. .. Anti-CCL28 Treatment of Sendai Virus-induced Airway Disease Mice were inoculated with SeV as above and injected subcutaneously with 200 μg of either anti-CCL28 (clone 134306; R&D Systems) or control rat IgG2b (clone 141945; R&D Systems) weekly from day 14 to 49 PI SeV. ..

    Control:

    Article Title: Structure-Function Analysis of CCL28 in the Development of Post-viral Asthma
    Article Snippet: On day 49 PI, mice were euthanized, and one lung was removed for mRNA isolation with TRIzol (Sigma). cDNA was generated with the QuantiTect reverse transcription kit (Qiagen) as per the manufacturer's instructions, and qPCR was performed using TaqMan fast master mix and the StepOnePlus PCR system (Applied Biosystems) to compare CCL28 (assay ID Mm00445039_m1) to GAPDH (assay ID 4352339E) copy number. .. Anti-CCL28 Treatment of Sendai Virus-induced Airway Disease Mice were inoculated with SeV as above and injected subcutaneously with 200 μg of either anti-CCL28 (clone 134306; R&D Systems) or control rat IgG2b (clone 141945; R&D Systems) weekly from day 14 to 49 PI SeV. ..

    Article Title: CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
    Article Snippet: We then sorted biotin-negative cells on an FACS Aria II cell sorter (Supplementary Fig. S5a) by using FACSDiva software (BD Biosciences). .. We used the following antibodies: anti-integrin-α7 (clone 334908, catalog FAB3518P; R&D Systems), anti-CD34 (clone 700011, catalog FAB6518G; R&D Systems), anti-CXCR4 (clone 247506, catalog FAB21651P; R&D Systems), isotype control Rat IgG2A (clone 141945, catalog IC013P; R&D Systems), and isotype control Rat IgG2B (clone 54447, positive for CD34 and integrin-α7. .. We generated and analyzed flow cytometry scatter plots by using FlowJo v8.7 (Tree Star, Ashland, USA).

    Biomarker Discovery:

    Article Title: An atypical atherogenic chemokine that promotes advanced atherosclerosis and hepatic lipogenesis
    Article Snippet: Anti-CXCR4 rabbit polyclonal antibody (Invitrogen, Cat# PA3-305) Anti-CD74 mouse monoclonal antibody (Santa Cruz Biotechnology Cat# sc-6262) ElisaAlexa Fluor 647-conjugated donkey anti-rat IgG (H+L) (Jackson ImmunoResearch Europe Ltd., Cat# 712-606-153) Alexa Fluor 488-conjugated goat anti-rat IgG (H+L) (Invitrogen; Cat# A-11006) Alexa Fluor 555-conjugated goat anti-rabbit IgG (H+L) (Invitrogen, Cat# A-21429) .. Anti-CD68 rat monoclonal antibody 1:100 (Bio-Rad Laboratories, Cat# MCA1957GA) Cy5-conjugated donkey Anti-Rat IgG (H+L) (Jackson ImmunoResearch Europe Ltd., Cat# 712-175-153) 4 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Internalization: Mouse PE-conjugated anti-CXCR4 antibody (R&D systems, Cat# FAB21651P) Rat PE-conjugated anti-mouse IgG2B Antibody (R&D systems, Cat# F0132) .. mouse anti-cMyc (9E10) mAb 1:200 (Santa Cruz, cat# sc-40) rabbit anti-DYKDDK Tag (D6W5B) mAb 1:200 (anti-FLAG, Cell Signaling Technologies, cat# 14793) LDL uptake: anti-human CD74 (LN2) (Santa Cruz, Cat# sc-6262) IgG1 $ light chain antibody (2B6) (Santa Cruz, Cat# sc-69914) Flow Cytometry: V450-conjugated rat anti-mouse CD45 (BD Biosciences Cat# 560501) FITC-conjugated anti-mouse CD3 (Miltenyi Biotec, Cat# 130-119-758) APC/Cy7-conjugated anti-mouse CD19 (BioLegend Cat# 115530) PE-conjugated anti-mouse CD11c (BioLegend, Cat# 117308) PE/Cy7-conjugated anti-mouse/human CD11b Antibody (BioLegend, Cat# 101216) APC-conjugated anti-mouse Ly-6C (BioLegend, Cat# 128016) PerCP-conjugated anti-mouse Ly-6G (BioLegend, Cat# 127654) APC/Cy7-cponjugated anti-human CD184 (CXCR4) (Biolegend, Cat# 306527) APC/C7-conjugated mouse anti-human IgG2a, $ Isotype Ctrl (Biolegend, Cat# 400229) FITC-conjugated mouse anti-human CD74 (BD Pharmingen, Cat# 555540) FITC-conjugated anti-human IgG2 (isotype control) (BD Pharmingen, Cat# 560952) • Anti-SREBP-1 mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-17755) Reactivity: Human; Host species: Mouse; Application: WB/IP/IHC/Elisa https://www.antibodyregistry.org/AB_628283 • Anti-SREBP-2 mouse monoclonal antibody (BD Biosciences, Cat# 557037) Reactivity: Human; Host species: Mouse; Application: WB/IP https://www.bdbiosciences.com/en-fi/products/reagents/western-blotting-and-molecular-reagents/purified-mouse-antisrebp-2.557037 • Anti-FASN mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-55580) Reactivity: Human; Host species: Mouse; Application: WB/IP/IHC/IF https://www.scbt.com/p/fatty-acid-synthase-antibody-a-5? gad_source=1&gclid=EAIaIQobChMIoojV5ayziQMV7IKDBx3jXBv9EAAYASAAEgLTB_D_BwE • Anti-LDLR mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-18823) Reactivity: Human; Host species: Mouse; Application: WB/IP/IHC/IF https://www.scbt.com/de/p/ldlr-antibody-c7?srsltid=AfmBOoqd0GsMZisONOaXooTHp3ZEweX2zBDuHTjU80zGSg46EYciP1ue • Anti-D-DT rabbit polyclonal antibody, generated by R. Bucala and colleagues Bucala lab (Yale) Reactivity: Human/mouse; Host species: Rabbit; Application: WB (Merk, M., Zierow, S., Leng, L., Das, R., et al. (2011).

    Conjugation Assay:

    Article Title: Monitoring Therapy Response of Experimental Arthritis with Radiolabeled Tracers Targeting Fibroblasts, Macrophages, or Integrin αvβ3.
    Article Snippet: .. MATERIALS AND METHODS Antibody Conjugation And RGD Peptide Rat anti‐mouse anti‐F4/80‐A3‐1 antibody (AbD Serotec, Kidlington, UK) and rat IgG2b (R&D Systems, Abingdon, UK) were dialyzed against 1:1 phosphate buffered saline (PBS, pH 7.4):water (1:1) to remove sodium azide. .. Antibodies 28H1 (anti‐FAP), DP47GS (8), anti‐F4/80‐A3‐1 and rat IgG2b (13), were conjugated with isothiocyanatobenzyl–diethylenetriamenepentaacetic acid (p‐SCN‐Bz‐DTPA) (Macrocyclics, Inc., Dallas, TX) in 0.1 M NaHCO3, pH 9.5, using a 5‐fold (28H1, DP47GS) or 10‐fold (anti‐ F4/80‐A3‐1, IgG2b) molar excess of p‐SCN‐Bz‐DTPA for 1 h at room temperature.

    Saline:

    Article Title: Monitoring Therapy Response of Experimental Arthritis with Radiolabeled Tracers Targeting Fibroblasts, Macrophages, or Integrin αvβ3.
    Article Snippet: .. MATERIALS AND METHODS Antibody Conjugation And RGD Peptide Rat anti‐mouse anti‐F4/80‐A3‐1 antibody (AbD Serotec, Kidlington, UK) and rat IgG2b (R&D Systems, Abingdon, UK) were dialyzed against 1:1 phosphate buffered saline (PBS, pH 7.4):water (1:1) to remove sodium azide. .. Antibodies 28H1 (anti‐FAP), DP47GS (8), anti‐F4/80‐A3‐1 and rat IgG2b (13), were conjugated with isothiocyanatobenzyl–diethylenetriamenepentaacetic acid (p‐SCN‐Bz‐DTPA) (Macrocyclics, Inc., Dallas, TX) in 0.1 M NaHCO3, pH 9.5, using a 5‐fold (28H1, DP47GS) or 10‐fold (anti‐ F4/80‐A3‐1, IgG2b) molar excess of p‐SCN‐Bz‐DTPA for 1 h at room temperature.



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