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rabbit phospho-eif2α antibody af1237  (Beyotime)


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    Structured Review

    Beyotime rabbit phospho-eif2α antibody af1237
    Rabbit Phospho Eif2α Antibody Af1237, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AF1237/eif2%CE%B1+antibody/pm39225067-75-4-16
    Average 90 stars, based on 1 article reviews
    rabbit phospho-eif2α antibody af1237 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) envelope protein (E) has been characterized as an important structural protein that plays critical roles in the interplay with its host to affect the virus life cycle.. Stress granules (SGs) are host translationally silent ribonucleoproteins, which are mainly induced by the phosphorylation of eIF2α in the PERK/eIF2α signaling pathway.. Our previous study found that PEDV E protein caused endoplasmic reticulum stress response (ERS)-mediated suppression of antiviral proteins’ translation.

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
    Article Snippet: Hoechst 33,342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), the kits of5,5′,6,6-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1), Pi-dium (PI), and DCFH-DA, N-Acetyl-L-cysteine (NAC) and the primary antibody against IRE-1α, p-IRE-1α, eIF-2α, p-eIF-2α, GRP78 were from Beyotime Biotechnology (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Inhibition of GZMB activity ameliorates cognitive dysfunction by reducing demyelination in diabetic mice.
    Article Snippet: After being blocked with bovine serum protein, the membranes were incubated with the primary antibodies against granzyme B (Abcam, ab255598), PERK (Wuhan Sanying Biotechnology, 24390-1-AP), pPERK (Shanghai Absin Biotechnology, abs172750), eIF2α(Wuhan Sanying Biotechnology, 11170-1-AP), p-eIF2α (Shanghai Beyotime Biotechnology, AF5803), NLRP3 (Abcm, ab214185), GSDMD-N (Abcam, ab219800), Caspase-1 (Jiangsu Affinity Biotechnology, AF5418), IL-1β (Beijing Bioss Biotechnology, 6319 R), IL-18 (Beijing Bioss Biotechnology, 0529 R), MBP (Abcam, ab7349), CNPase (Abcam, ab6319), or β-actin (Beijing Bioss Biotechnology, 0061 R).

    Western Blot:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) envelope protein (E) has been characterized as an important structural protein that plays critical roles in the interplay with its host to affect the virus life cycle.. Stress granules (SGs) are host translationally silent ribonucleoproteins, which are mainly induced by the phosphorylation of eIF2α in the PERK/eIF2α signaling pathway.. Our previous study found that PEDV E protein caused endoplasmic reticulum stress response (ERS)-mediated suppression of antiviral proteins’ translation.

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
    Article Snippet: Hoechst 33,342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), the kits of5,5′,6,6-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1), Pi-dium (PI), and DCFH-DA, N-Acetyl-L-cysteine (NAC) and the primary antibody against IRE-1α, p-IRE-1α, eIF-2α, p-eIF-2α, GRP78 were from Beyotime Biotechnology (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Inhibition of GZMB activity ameliorates cognitive dysfunction by reducing demyelination in diabetic mice.
    Article Snippet: After being blocked with bovine serum protein, the membranes were incubated with the primary antibodies against granzyme B (Abcam, ab255598), PERK (Wuhan Sanying Biotechnology, 24390-1-AP), pPERK (Shanghai Absin Biotechnology, abs172750), eIF2α(Wuhan Sanying Biotechnology, 11170-1-AP), p-eIF2α (Shanghai Beyotime Biotechnology, AF5803), NLRP3 (Abcm, ab214185), GSDMD-N (Abcam, ab219800), Caspase-1 (Jiangsu Affinity Biotechnology, AF5418), IL-1β (Beijing Bioss Biotechnology, 6319 R), IL-18 (Beijing Bioss Biotechnology, 0529 R), MBP (Abcam, ab7349), CNPase (Abcam, ab6319), or β-actin (Beijing Bioss Biotechnology, 0061 R).

    Activation Assay:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) envelope protein (E) has been characterized as an important structural protein that plays critical roles in the interplay with its host to affect the virus life cycle.. Stress granules (SGs) are host translationally silent ribonucleoproteins, which are mainly induced by the phosphorylation of eIF2α in the PERK/eIF2α signaling pathway.. Our previous study found that PEDV E protein caused endoplasmic reticulum stress response (ERS)-mediated suppression of antiviral proteins’ translation.

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
    Article Snippet: Hoechst 33,342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), the kits of5,5′,6,6-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1), Pi-dium (PI), and DCFH-DA, N-Acetyl-L-cysteine (NAC) and the primary antibody against IRE-1α, p-IRE-1α, eIF-2α, p-eIF-2α, GRP78 were from Beyotime Biotechnology (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Inhibition of GZMB activity ameliorates cognitive dysfunction by reducing demyelination in diabetic mice.
    Article Snippet: After being blocked with bovine serum protein, the membranes were incubated with the primary antibodies against granzyme B (Abcam, ab255598), PERK (Wuhan Sanying Biotechnology, 24390-1-AP), pPERK (Shanghai Absin Biotechnology, abs172750), eIF2α(Wuhan Sanying Biotechnology, 11170-1-AP), p-eIF2α (Shanghai Beyotime Biotechnology, AF5803), NLRP3 (Abcm, ab214185), GSDMD-N (Abcam, ab219800), Caspase-1 (Jiangsu Affinity Biotechnology, AF5418), IL-1β (Beijing Bioss Biotechnology, 6319 R), IL-18 (Beijing Bioss Biotechnology, 0529 R), MBP (Abcam, ab7349), CNPase (Abcam, ab6319), or β-actin (Beijing Bioss Biotechnology, 0061 R).

    Control:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) envelope protein (E) has been characterized as an important structural protein that plays critical roles in the interplay with its host to affect the virus life cycle.. Stress granules (SGs) are host translationally silent ribonucleoproteins, which are mainly induced by the phosphorylation of eIF2α in the PERK/eIF2α signaling pathway.. Our previous study found that PEDV E protein caused endoplasmic reticulum stress response (ERS)-mediated suppression of antiviral proteins’ translation.

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
    Article Snippet: Hoechst 33,342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), the kits of5,5′,6,6-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1), Pi-dium (PI), and DCFH-DA, N-Acetyl-L-cysteine (NAC) and the primary antibody against IRE-1α, p-IRE-1α, eIF-2α, p-eIF-2α, GRP78 were from Beyotime Biotechnology (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Inhibition of GZMB activity ameliorates cognitive dysfunction by reducing demyelination in diabetic mice.
    Article Snippet: After being blocked with bovine serum protein, the membranes were incubated with the primary antibodies against granzyme B (Abcam, ab255598), PERK (Wuhan Sanying Biotechnology, 24390-1-AP), pPERK (Shanghai Absin Biotechnology, abs172750), eIF2α(Wuhan Sanying Biotechnology, 11170-1-AP), p-eIF2α (Shanghai Beyotime Biotechnology, AF5803), NLRP3 (Abcm, ab214185), GSDMD-N (Abcam, ab219800), Caspase-1 (Jiangsu Affinity Biotechnology, AF5418), IL-1β (Beijing Bioss Biotechnology, 6319 R), IL-18 (Beijing Bioss Biotechnology, 0529 R), MBP (Abcam, ab7349), CNPase (Abcam, ab6319), or β-actin (Beijing Bioss Biotechnology, 0061 R).

    Blocking Assay:

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) envelope protein (E) has been characterized as an important structural protein that plays critical roles in the interplay with its host to affect the virus life cycle.. Stress granules (SGs) are host translationally silent ribonucleoproteins, which are mainly induced by the phosphorylation of eIF2α in the PERK/eIF2α signaling pathway.. Our previous study found that PEDV E protein caused endoplasmic reticulum stress response (ERS)-mediated suppression of antiviral proteins’ translation.

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
    Article Snippet: Hoechst 33,342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), the kits of5,5′,6,6-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1), Pi-dium (PI), and DCFH-DA, N-Acetyl-L-cysteine (NAC) and the primary antibody against IRE-1α, p-IRE-1α, eIF-2α, p-eIF-2α, GRP78 were from Beyotime Biotechnology (Shanghai, China).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Inhibition of GZMB activity ameliorates cognitive dysfunction by reducing demyelination in diabetic mice.
    Article Snippet: After being blocked with bovine serum protein, the membranes were incubated with the primary antibodies against granzyme B (Abcam, ab255598), PERK (Wuhan Sanying Biotechnology, 24390-1-AP), pPERK (Shanghai Absin Biotechnology, abs172750), eIF2α(Wuhan Sanying Biotechnology, 11170-1-AP), p-eIF2α (Shanghai Beyotime Biotechnology, AF5803), NLRP3 (Abcm, ab214185), GSDMD-N (Abcam, ab219800), Caspase-1 (Jiangsu Affinity Biotechnology, AF5418), IL-1β (Beijing Bioss Biotechnology, 6319 R), IL-18 (Beijing Bioss Biotechnology, 0529 R), MBP (Abcam, ab7349), CNPase (Abcam, ab6319), or β-actin (Beijing Bioss Biotechnology, 0061 R).



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    Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, <t>p-eIF2α,</t> and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.
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    Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, p-eIF2α, and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, p-eIF2α, and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Quantitative RT-PCR, Western Blot, TUNEL Assay

    Cereulide triggers apoptosis by mediating ER stress in HEK293T cells and HepaRG cells. ( A , B ) Cytotoxicity of cereulide in HepaRG and HEK293T cells. HepaRG and HEK293T cells were treated with different concentrations of cereulide for 24 h. Cell viability was then determined with CCK-8 ( n = 5). ( C ) Cell apoptosis resulting from cereulide treatment in HepaRG and HEK293T cells. HepaRG and HEK293T cells were evaluated with 0.3 and 1 ng/mL cereulide by flow cytometry. The data were analyzed using FlowJo software ( n = 3). ( D , E ) Representative transmission electron microscopy images of HepaRG and HEK293T cells treated with cereulide toxin. Cereulide treated HepaRG and HEK293T cells for 24 h, and observed by transmission electron microscope. M indicates mitochondria. Scale bars: left panel, 2 µm; right panel, 500 nm. ( F , G ) ER labeling. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h. Image magnification: ×200; scale bar = 100 µm. The cells were stained with ER-Tracker and observed under a fluorescence microscope at 587 nm excitation and 615 nm emission. ( H , I ) ER stress-related protein levels during cereulide exposure were evaluated in HepaRG and HEK293T cells. HepaRG and HEK293T cells were exposed to cereulide toxin (0, 0.3, 1 ng/mL) for 3, 6 and 24 h and Western blot analysis for BiP, ATF6, IRE1α, p-IRE1α, XBP1(s), PERK, p-PERK, p-eIF2α, CHOP. ( J ) IRE1α knock-down blocked cereulide to down-regulate XBP1s. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then exposed to cereulide (1 ng/mL) for 6 h. ( K ) IRE1α knock-down blocked cereulide to increase in apoptosis. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then evaluated with 1 ng/mL cereulide for 24 h by flow cytometry ( n = 3).

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Cereulide triggers apoptosis by mediating ER stress in HEK293T cells and HepaRG cells. ( A , B ) Cytotoxicity of cereulide in HepaRG and HEK293T cells. HepaRG and HEK293T cells were treated with different concentrations of cereulide for 24 h. Cell viability was then determined with CCK-8 ( n = 5). ( C ) Cell apoptosis resulting from cereulide treatment in HepaRG and HEK293T cells. HepaRG and HEK293T cells were evaluated with 0.3 and 1 ng/mL cereulide by flow cytometry. The data were analyzed using FlowJo software ( n = 3). ( D , E ) Representative transmission electron microscopy images of HepaRG and HEK293T cells treated with cereulide toxin. Cereulide treated HepaRG and HEK293T cells for 24 h, and observed by transmission electron microscope. M indicates mitochondria. Scale bars: left panel, 2 µm; right panel, 500 nm. ( F , G ) ER labeling. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h. Image magnification: ×200; scale bar = 100 µm. The cells were stained with ER-Tracker and observed under a fluorescence microscope at 587 nm excitation and 615 nm emission. ( H , I ) ER stress-related protein levels during cereulide exposure were evaluated in HepaRG and HEK293T cells. HepaRG and HEK293T cells were exposed to cereulide toxin (0, 0.3, 1 ng/mL) for 3, 6 and 24 h and Western blot analysis for BiP, ATF6, IRE1α, p-IRE1α, XBP1(s), PERK, p-PERK, p-eIF2α, CHOP. ( J ) IRE1α knock-down blocked cereulide to down-regulate XBP1s. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then exposed to cereulide (1 ng/mL) for 6 h. ( K ) IRE1α knock-down blocked cereulide to increase in apoptosis. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then evaluated with 1 ng/mL cereulide for 24 h by flow cytometry ( n = 3).

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: CCK-8 Assay, Flow Cytometry, Software, Transmission Assay, Electron Microscopy, Microscopy, Labeling, Staining, Fluorescence, Western Blot, Transfection, Negative Control

    Cereulide induces ER Stress response by ROS production. ( A , B ) Cereulide causes an increase in ROS production. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h, the fluorescence of dichlorofluorescindiacetate (DCF) were detected at 488 nm excitation and 525 nm emission; n = 5. ( C , D ) Effects of ROS inhibitor on ROS production after cereulide treatment. HEK293T cells were pretreated with 5 mM NAC followed by treatment of 0.5 ng/mL cereulide for 2, 4, 24 h. Cytosolic ROS production measured by DHE labeling, n = 5. ( E , F ) HepaRG cells were pretreated with 7 mM NAC followed by treatment of 1 ng/mL cereulide for 2, 4, 24 h; n = 5. For C and E panels, image magnification: ×400; scale bar = 50 µm. ( G , H ) ROS inhibitor relieves ER stress caused by cereulide. According to the dosage described above, HepaRG and HEK293T cells were treated for 4 h and Western blot analysis for p-eIF2α, CHOP. ( I ) NAC treatment reduce the apoptosis caused by cereulide. HepaRG and HEK293T cells were treated as described above and then evaluated by flow cytometry; n = 3. For all panels, * p < 0.05 and ** p < 0.01 compared to the control cells; # p < 0.05 and ## p < 0.01 compared to NAC treated cells.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Cereulide induces ER Stress response by ROS production. ( A , B ) Cereulide causes an increase in ROS production. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h, the fluorescence of dichlorofluorescindiacetate (DCF) were detected at 488 nm excitation and 525 nm emission; n = 5. ( C , D ) Effects of ROS inhibitor on ROS production after cereulide treatment. HEK293T cells were pretreated with 5 mM NAC followed by treatment of 0.5 ng/mL cereulide for 2, 4, 24 h. Cytosolic ROS production measured by DHE labeling, n = 5. ( E , F ) HepaRG cells were pretreated with 7 mM NAC followed by treatment of 1 ng/mL cereulide for 2, 4, 24 h; n = 5. For C and E panels, image magnification: ×400; scale bar = 50 µm. ( G , H ) ROS inhibitor relieves ER stress caused by cereulide. According to the dosage described above, HepaRG and HEK293T cells were treated for 4 h and Western blot analysis for p-eIF2α, CHOP. ( I ) NAC treatment reduce the apoptosis caused by cereulide. HepaRG and HEK293T cells were treated as described above and then evaluated by flow cytometry; n = 3. For all panels, * p < 0.05 and ** p < 0.01 compared to the control cells; # p < 0.05 and ## p < 0.01 compared to NAC treated cells.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Fluorescence, Labeling, Western Blot, Flow Cytometry

    NaB relieves cereulide-induced liver and kidney damage by inhibiting ER stress. ( A ) Representative images of H&E-stained liver and kidney tissues from cereulide + NaB-treated mice and cereulide-treated mice (×200 magnification). ( B , C ) The mRNA levels of TNF-α, IL-6 and IL-10 were detected in liver and kidney tissues by RT-qPCR, n = 5. ( D , E ) Western blot analysis for XBP1(s), p-eIF2α, CHOP and Caspase3 in the liver and kidney tissues of mice that exposed to cereulide (50 μg/kg) were treated with NaB (100 mg/kg) for 28 days. ( F , G ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (50 μg/kg) and cereulide + NaB (100 mg/kg) (×400 magnification). Arrows indicated apoptotic cells. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05 and ** p < 0.01 compared to the control group; # p < 0.05 and ## p < 0.01 compared to cereulide + NaB group.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: NaB relieves cereulide-induced liver and kidney damage by inhibiting ER stress. ( A ) Representative images of H&E-stained liver and kidney tissues from cereulide + NaB-treated mice and cereulide-treated mice (×200 magnification). ( B , C ) The mRNA levels of TNF-α, IL-6 and IL-10 were detected in liver and kidney tissues by RT-qPCR, n = 5. ( D , E ) Western blot analysis for XBP1(s), p-eIF2α, CHOP and Caspase3 in the liver and kidney tissues of mice that exposed to cereulide (50 μg/kg) were treated with NaB (100 mg/kg) for 28 days. ( F , G ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (50 μg/kg) and cereulide + NaB (100 mg/kg) (×400 magnification). Arrows indicated apoptotic cells. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05 and ** p < 0.01 compared to the control group; # p < 0.05 and ## p < 0.01 compared to cereulide + NaB group.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Staining, Quantitative RT-PCR, Western Blot, TUNEL Assay