cycloheximide 94271 (Amresco)
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Cycloheximide 94271, supplied by Amresco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/94271/cycloheximide+94271/pm38701646-357-12-14
Average 90 stars, based on 1 article reviews
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Cells were incubated in EMM5S overnight until OD600 reached 0.6–0.8, 100 μg/mL Inhibition:Article Title: CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation. Article Snippet: For the S-phase arrest-induced centriole overduplication assays, 2 mM hydroxyurea (#H8627, Sigma-Aldrich) was added to U2OS cells for 36 h, or 1.5 mg/mL aphidicolin (#178273, Millipore) was added to HeLa cells for 36 h. For inhibition of HDAC or SIRT1 activity, HeLa or U2OS cells were treated with 100 ng/mL trichostatin (#T1952, Sigma-Aldrich) or 1 mM Ex-527 (#S1541, Selleckchem), respectively, for 48 h. For inhibition of NAT10 activity, HeLa cells were treated with 20 mM remodelin (#S7641, Selleckchem) for 48 h. For inhibition of proteasome activity, HeLa cells were treated with 50 mg/mL MG132 (#M8699, Sigma-Aldrich) for 6 h. For measurement of the HsSAS-6 half-life, HeLa cells were treated with 50 mg/mL cycloheximide (#94271, Amresco). .. For the S-phase arrest-induced centriole overduplication assays, 2 mM hydroxyurea (#H8627, Sigma-Aldrich) was added to U2OS cells for 36 h, or 1.5 mg/mL aphidicolin (#178273, Millipore) was added to HeLa cells for 36 h. For inhibition of HDAC or SIRT1 activity, HeLa or U2OS cells were treated with 100 ng/mL trichostatin (#T1952, Sigma-Aldrich) or 1 mM Ex-527 (#S1541, Selleckchem), respectively, for 48 h. For inhibition of NAT10 activity, HeLa cells were treated with 20 mM remodelin (#S7641, Selleckchem) for 48 h. For inhibition of proteasome activity, HeLa cells were treated with 50 mg/mL MG132 (#M8699, Sigma-Aldrich) for 6 h. For measurement of the HsSAS-6 half-life, HeLa cells were treated with 50 mg/mL Activity Assay:Article Title: CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation. Article Snippet: For the S-phase arrest-induced centriole overduplication assays, 2 mM hydroxyurea (#H8627, Sigma-Aldrich) was added to U2OS cells for 36 h, or 1.5 mg/mL aphidicolin (#178273, Millipore) was added to HeLa cells for 36 h. For inhibition of HDAC or SIRT1 activity, HeLa or U2OS cells were treated with 100 ng/mL trichostatin (#T1952, Sigma-Aldrich) or 1 mM Ex-527 (#S1541, Selleckchem), respectively, for 48 h. For inhibition of NAT10 activity, HeLa cells were treated with 20 mM remodelin (#S7641, Selleckchem) for 48 h. For inhibition of proteasome activity, HeLa cells were treated with 50 mg/mL MG132 (#M8699, Sigma-Aldrich) for 6 h. For measurement of the HsSAS-6 half-life, HeLa cells were treated with 50 mg/mL cycloheximide (#94271, Amresco). .. For the S-phase arrest-induced centriole overduplication assays, 2 mM hydroxyurea (#H8627, Sigma-Aldrich) was added to U2OS cells for 36 h, or 1.5 mg/mL aphidicolin (#178273, Millipore) was added to HeLa cells for 36 h. 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