pframa bio his (Addgene inc)

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Addgene inc pframa bio his
Pframa Bio His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/50737/RAMA-bio-His+(Plasmid+%2350737)/pmc12064020-66-18-19
Average 93 stars, based on 4 article reviews

pframa bio his - by Bioz Stars, 2026-10

93/100 stars

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Recombinant:

Article Title: Atypical memory B cell frequency correlates with antibody breadth and function in malaria immune adults
Article Snippet: .. Recombinant extracellular domains of merozoite antigens MSRP5-bio-his (Addgene plasmid #50805), SERA9-bio-his (Addgene plasmid #50820), RAMA-bio-his (Addgene plasmid #50737), and CyRPA-bio-his ( PF D1130w-bio-his, Addgene plasmid #50823) were gifts from Dr. Gavin Wright (University of Oxford, Oxford, UK) . .. Plasmids were transiently expressed using the Expi293 Expression System as described previously .

Article Title: Deposition of complement regulators on the surface of Plasmodium falciparum merozoites depends on the immune status of the host
Article Snippet: .. Plasmids encoding the recombinant merozoite antigens PfMSRP5-bio-his (Addgene plasmid #50805), PfSERA9-bio-his (Addgene plasmid #50820), PfMSP3-bio (Addgene plasmid #47731), PfRAMA-bio-his (Addgene plasmid #50737), Pf92-bio (Addgene plasmid #47728) and PfCyRPA-bio-his (PFD1130w-bio-his, Addgene plasmid #50823) were gifts from Dr. Gavin Wright, University of Oxford, Oxford, UK [ , ]. .. The plasmids were used for protein expression using the Exp293 expression system (Invitrogen), following manufacturer instructions.

Article Title: Deposition of complement regulators on the surface of Plasmodium falciparum merozoites depends on the immune status of the host.
Article Snippet: .. Plasmids encoding the recombinant merozoite antigens PfMSRP5-bio-his (Addgene plasmid #50805), PfSERA9-biohis (Addgene plasmid #50820), PfMSP3-bio (Addgene plasmid #47731), PfRAMA-bio-his (Addgene plasmid #50737), Pf92-bio (Addgene plasmid #47728) and PfCyRPA-bio-his (PFD1130w-bio-his, Addgene plasmid #50823) were gifts from Dr. Gavin Wright, University of Oxford, Oxford, UK [29,30]. .. The plasmids were used for protein expression using the Exp293 expression system (Invitrogen), following manufacturer instructions.

Article Title: Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children
Article Snippet: .. Recombinant extracellular domains of merozoite antigens PfMSRP5-bio-his (Addgene plasmid #50805), PfSERA9-bio-his (Addgene plasmid #50820), PfRAMA-bio-his (Addgene plasmid #50737), and PfCyRPA-bio-his (PFD1130w-bio-his, Addgene plasmid #50823) were gifts from Dr. Gavin Wright (University of Oxford, Oxford, UK) [ ]. .. Transient transfection of expression plasmids was performed using the Expi293F TM Expression System Kit (ThermoFisher) as per manufacturer’s instructions.

Plasmid Preparation:

Article Title: Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen
Article Snippet: .. The plasmid encoding the P. falciparum rhoptry antigen PfRAMA-bio-his (Addgene, plasmid #50737) was a gift from Gavin Wright (Wellcome Sanger Institute, Cambridge, UK). ..

Article Title: Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen
Article Snippet: After blocking, PfRAMA was detected by our panel of mouse anti-PfRAMA mAbs (5 μg/mL) and incubated for 1 h. This step was followed by adding a secondary rabbit anti-mouse immunoglobulin—HRP conjugated antibody (DAKO) for 1 h. After washing the membrane, western blots were developed by LumiGLO peroxidase chemiluminescent substrate (SeraCare) and images were acquired using the ChemiDoc MP Imaging System (Bio-rad). .. The gene encoding PfRAMA was amplified from the Addgene plasmid #50737 using Phusion HF PCR Mastermix (NEB) and purified using QIAquick PCR Purification Kit (QIAGEN). .. Next, 5 μg of PCR product was digested with 1 unit of DNaseI (NEB) for 1 min and 45 s. The yielded gene fragments were run on a 1% agarose gel and the smears located between 50 and 250 bp were cut out and purified using QIAX II Gel Extraction Kit (QIAGEN).

Amplification:

Article Title: Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen
Article Snippet: After blocking, PfRAMA was detected by our panel of mouse anti-PfRAMA mAbs (5 μg/mL) and incubated for 1 h. This step was followed by adding a secondary rabbit anti-mouse immunoglobulin—HRP conjugated antibody (DAKO) for 1 h. After washing the membrane, western blots were developed by LumiGLO peroxidase chemiluminescent substrate (SeraCare) and images were acquired using the ChemiDoc MP Imaging System (Bio-rad). .. The gene encoding PfRAMA was amplified from the Addgene plasmid #50737 using Phusion HF PCR Mastermix (NEB) and purified using QIAquick PCR Purification Kit (QIAGEN). .. Next, 5 μg of PCR product was digested with 1 unit of DNaseI (NEB) for 1 min and 45 s. The yielded gene fragments were run on a 1% agarose gel and the smears located between 50 and 250 bp were cut out and purified using QIAX II Gel Extraction Kit (QIAGEN).

Polymerase Chain Reaction:

Article Title: Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen
Article Snippet: After blocking, PfRAMA was detected by our panel of mouse anti-PfRAMA mAbs (5 μg/mL) and incubated for 1 h. This step was followed by adding a secondary rabbit anti-mouse immunoglobulin—HRP conjugated antibody (DAKO) for 1 h. After washing the membrane, western blots were developed by LumiGLO peroxidase chemiluminescent substrate (SeraCare) and images were acquired using the ChemiDoc MP Imaging System (Bio-rad). .. The gene encoding PfRAMA was amplified from the Addgene plasmid #50737 using Phusion HF PCR Mastermix (NEB) and purified using QIAquick PCR Purification Kit (QIAGEN). .. Next, 5 μg of PCR product was digested with 1 unit of DNaseI (NEB) for 1 min and 45 s. The yielded gene fragments were run on a 1% agarose gel and the smears located between 50 and 250 bp were cut out and purified using QIAX II Gel Extraction Kit (QIAGEN).

Purification:

Article Title: Enhancing neutralization of Plasmodium falciparum using a novel monoclonal antibody against the rhoptry-associated membrane antigen
Article Snippet: After blocking, PfRAMA was detected by our panel of mouse anti-PfRAMA mAbs (5 μg/mL) and incubated for 1 h. This step was followed by adding a secondary rabbit anti-mouse immunoglobulin—HRP conjugated antibody (DAKO) for 1 h. After washing the membrane, western blots were developed by LumiGLO peroxidase chemiluminescent substrate (SeraCare) and images were acquired using the ChemiDoc MP Imaging System (Bio-rad). .. The gene encoding PfRAMA was amplified from the Addgene plasmid #50737 using Phusion HF PCR Mastermix (NEB) and purified using QIAquick PCR Purification Kit (QIAGEN). .. Next, 5 μg of PCR product was digested with 1 unit of DNaseI (NEB) for 1 min and 45 s. The yielded gene fragments were run on a 1% agarose gel and the smears located between 50 and 250 bp were cut out and purified using QIAX II Gel Extraction Kit (QIAGEN).

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