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recombinant mouse p selectin ligand  (Sino Biological)


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    Structured Review

    Sino Biological recombinant mouse p selectin ligand
    B.PSel-Crry and NB.PSel-Crry reduce inflammation markers after hindlimb ischemia and reperfusion. A murine hindlimb IRI model was used to evaluate the effect of B.PSel and NB.PSel administered i.v. upon reperfusion after 2 h ischemia. (A) Representative images of sections stained for MPO. At the higher dose of 0.5 mg, there were lower numbers of infiltrating myeloid cells in mice treated with B.PSel-Crry compared to NB.PSel-Crry. Scale bars, 50μm. (B) Quantification of infiltrating myeloid cells performed by assessing the number of MPO-positive cells per field. Parametric One-way ANOVA with Tukey’s correction. (C) Representative images of sections stained for <t>P-selectin.</t> Scale bars, 50μm. (D) Quantification of P-selectin expression, which was significantly lower in B.PSel-Crry treated mice compared to NB.PSel-Crry treated mice at both concentrations. Parametric One-Way ANOVA with Tukey’s correction. Quantification data expressed as Mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. n = 5 for all groups.
    Recombinant Mouse P Selectin Ligand, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/50737/Mouse+SELP+%2F+selectin+P+%2F+P-selectin+Protein/pmc08654931-41-17-21
    Average 93 stars, based on 3 article reviews
    recombinant mouse p selectin ligand - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation"

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.785229

    B.PSel-Crry and NB.PSel-Crry reduce inflammation markers after hindlimb ischemia and reperfusion. A murine hindlimb IRI model was used to evaluate the effect of B.PSel and NB.PSel administered i.v. upon reperfusion after 2 h ischemia. (A) Representative images of sections stained for MPO. At the higher dose of 0.5 mg, there were lower numbers of infiltrating myeloid cells in mice treated with B.PSel-Crry compared to NB.PSel-Crry. Scale bars, 50μm. (B) Quantification of infiltrating myeloid cells performed by assessing the number of MPO-positive cells per field. Parametric One-way ANOVA with Tukey’s correction. (C) Representative images of sections stained for P-selectin. Scale bars, 50μm. (D) Quantification of P-selectin expression, which was significantly lower in B.PSel-Crry treated mice compared to NB.PSel-Crry treated mice at both concentrations. Parametric One-Way ANOVA with Tukey’s correction. Quantification data expressed as Mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. n = 5 for all groups.
    Figure Legend Snippet: B.PSel-Crry and NB.PSel-Crry reduce inflammation markers after hindlimb ischemia and reperfusion. A murine hindlimb IRI model was used to evaluate the effect of B.PSel and NB.PSel administered i.v. upon reperfusion after 2 h ischemia. (A) Representative images of sections stained for MPO. At the higher dose of 0.5 mg, there were lower numbers of infiltrating myeloid cells in mice treated with B.PSel-Crry compared to NB.PSel-Crry. Scale bars, 50μm. (B) Quantification of infiltrating myeloid cells performed by assessing the number of MPO-positive cells per field. Parametric One-way ANOVA with Tukey’s correction. (C) Representative images of sections stained for P-selectin. Scale bars, 50μm. (D) Quantification of P-selectin expression, which was significantly lower in B.PSel-Crry treated mice compared to NB.PSel-Crry treated mice at both concentrations. Parametric One-Way ANOVA with Tukey’s correction. Quantification data expressed as Mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. n = 5 for all groups.

    Techniques Used: Staining, Expressing

    Related Articles

    SPR Assay:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Binding Assay:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Recombinant:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Article Title: A pyroptosis-enhanced leucocyte-hitchhiking liposomal nanoplatform for potentiated immunotherapy of hepatocellular carcinoma
    Article Snippet: Hepatocellular carcinoma (HCC) is a highly malignant tumor with unsatisfactory response to immunotherapy.. Pyroptosis, a recently discovered form of regulated cell death (RCD), possesses a huge potential to enhance the immunotherapy efficiency against HCC.. To achieve efficient drug delivery and ideal activation of antitumor immunity, an E-selectin modified liposomal nanoplatform co-loading gemcitabine elaidate and BMS-202 (a small molecule programmed cell death protein 1 (PD-1)/programmed death ligand 1 (PD-L1) inhibitor) was designed.



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    Image Search Results


    DUB-IN-3 inhibits ATC progression by activating ferroptosis. (A-C) The CCK8 assay (A), colony formation assay (B), and transwell assay (C) was applied to present the effect of DUB-IN-3 on the proliferation, colony formation, and migration capability of BHT101 cells. (D) The CCK8 assay was used to assess the responses of BHT101 cells with DUB-IN-3 treatment to RSL-3 ± Fer-1. (E, F) Lipid ROS (E) and GSH (F) levels in DUB-IN-3 treating ATC cells. (G) The parental BHT101 cells were injected into the right underarm of nude mouse, then DUB-IN-3 was intraperitoneally injected every 3 days ( n = 3 mouse per group). The tumors were removed and weighted after 17 days. (H) Representative images of IHC staining for Ki-67. (I) GPR34 stabilized by deubiquitinase USP8 promotes ATC progression via inhibiting ferroptosis. Ns, nonsignificant. p > 0.05, ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, ⁣ ∗∗∗ p < 0.001.

    Journal: Mediators of Inflammation

    Article Title: GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC

    doi: 10.1155/mi/5576056

    Figure Lengend Snippet: DUB-IN-3 inhibits ATC progression by activating ferroptosis. (A-C) The CCK8 assay (A), colony formation assay (B), and transwell assay (C) was applied to present the effect of DUB-IN-3 on the proliferation, colony formation, and migration capability of BHT101 cells. (D) The CCK8 assay was used to assess the responses of BHT101 cells with DUB-IN-3 treatment to RSL-3 ± Fer-1. (E, F) Lipid ROS (E) and GSH (F) levels in DUB-IN-3 treating ATC cells. (G) The parental BHT101 cells were injected into the right underarm of nude mouse, then DUB-IN-3 was intraperitoneally injected every 3 days ( n = 3 mouse per group). The tumors were removed and weighted after 17 days. (H) Representative images of IHC staining for Ki-67. (I) GPR34 stabilized by deubiquitinase USP8 promotes ATC progression via inhibiting ferroptosis. Ns, nonsignificant. p > 0.05, ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, ⁣ ∗∗∗ p < 0.001.

    Article Snippet: DUB-IN-3 (HY-50737-11866) were obtained from MedChemExpress.

    Techniques: CCK-8 Assay, Colony Assay, Transwell Assay, Migration, Injection, Immunohistochemistry