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custom 244k gene expression microarray  (Agilent technologies)


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    Agilent technologies custom 244k gene expression microarray
    Custom 244k Gene Expression Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/244k+microarray/us11549152-786-8-9
    Average 90 stars, based on 1 article reviews
    custom 244k gene expression microarray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    CpG Methylation Assay:

    Article Title: Identification and validation of PCDHGA12 and PRRX1 methylation for detecting lung cancer in bronchial washing sample
    Article Snippet: .. CpG methylation microarray analysis was performed as described previously ( ) using human CpG island microarray kit, 244k (Agilent Technologies, Inc.) according to the manufacturer's instructions. ..

    Microarray:

    Article Title: Identification and validation of PCDHGA12 and PRRX1 methylation for detecting lung cancer in bronchial washing sample
    Article Snippet: .. CpG methylation microarray analysis was performed as described previously ( ) using human CpG island microarray kit, 244k (Agilent Technologies, Inc.) according to the manufacturer's instructions. ..

    Article Title: Tumor Transcriptome Sequencing Reveals Allelic Expression Imbalances Associated with Copy Number Alterations
    Article Snippet: .. aCGH was performed using the Agilent Human Genome Microarray Kit 244K (Agilent Technologies, Santa Clara, CA) which contains ∼244,000 60-mer oligonucleotide probes spanning coding and non-coding genomic sequences with median spacing of 7.4 and 16.5 kb respectively. .. Arrays were analyzed using the Genepix 4200A scanner (Axon Instruments, Union City, CA) and the Agilent Feature Extraction software (v9.1).

    Immunoprecipitation:

    Article Title: EWS/FLI Mediates Transcriptional Repression via NKX2.2 during Oncogenic Transformation in Ewing ' s Sarcoma
    Article Snippet: .. Cross-linked DNA/protein complexes were immunoprecipitated using an anti-FLAG antibody and recovered DNA was hybridized to Agilent 244k promoter microarrays. ..

    other:

    Article Title: Products for assessing colorectal cancer molecular subtype and risk of recurrence and for determining and administering treatment protocols based thereon
    Article Snippet: Sample and reference can be co-hybridized on a Custom Agilent 244K Gene Expression Microarray.

    Sequencing:

    Article Title: Patient-derived breast tumor xenografts facilitating personalized cancer therapy
    Article Snippet: Genetic stability across generations , , , , Yes, CGH and Affymetrix microarray , , Yes, Agilent and SNP microarray , Yes, genomic, transciptomic, proteomic , , . .. Genomic , DNA copy number alterations: 14/18 pairs of tumors shared more than 56% copy number alterations, unsupervised hierarchical clustering showed 16/18 pairs segregated together. Recurrent changes between patient tumors and xenografts showed losses in 176 chromosomal regions and gains in 202 chromosomal regions , CGH array Agilent 244K human genome CGH microarrays. CGH results showed shared alterations between primary and xenograft tumors with more pronounced alterations in engrafted tumors, that is, TP53 patient 1 wild-type allele, xenograft LOH; ER+ tumor gained basal-like alterations , Paired-end sequencing to achieve deep coverage of patient blood, tumor, metastasis, and xenografted tumor Confirmed SNP coverage by Illumina 1M duo arrays. The PDX retained primary tumor mutations and showed enrichment of mutations similar to patient metastasis , Genome-wide SNPs with enhancement of existing aberrations , , . .. Microarray-gene expression , Gene expression analysis (GEA) , Agilent GEA High dose E2 supplementation altered gene expression , PAM50 intrinsic subtype classification , Agilent GEA, intrinsic subtype classification, PAM50 confirmation , Agilent GEA, PAM50 intrinsic subtype classification , Agilent GEA, PAM50 intrinsic subtype classification.

    Genome Wide:

    Article Title: Patient-derived breast tumor xenografts facilitating personalized cancer therapy
    Article Snippet: Genetic stability across generations , , , , Yes, CGH and Affymetrix microarray , , Yes, Agilent and SNP microarray , Yes, genomic, transciptomic, proteomic , , . .. Genomic , DNA copy number alterations: 14/18 pairs of tumors shared more than 56% copy number alterations, unsupervised hierarchical clustering showed 16/18 pairs segregated together. Recurrent changes between patient tumors and xenografts showed losses in 176 chromosomal regions and gains in 202 chromosomal regions , CGH array Agilent 244K human genome CGH microarrays. CGH results showed shared alterations between primary and xenograft tumors with more pronounced alterations in engrafted tumors, that is, TP53 patient 1 wild-type allele, xenograft LOH; ER+ tumor gained basal-like alterations , Paired-end sequencing to achieve deep coverage of patient blood, tumor, metastasis, and xenografted tumor Confirmed SNP coverage by Illumina 1M duo arrays. The PDX retained primary tumor mutations and showed enrichment of mutations similar to patient metastasis , Genome-wide SNPs with enhancement of existing aberrations , , . .. Microarray-gene expression , Gene expression analysis (GEA) , Agilent GEA High dose E2 supplementation altered gene expression , PAM50 intrinsic subtype classification , Agilent GEA, intrinsic subtype classification, PAM50 confirmation , Agilent GEA, PAM50 intrinsic subtype classification , Agilent GEA, PAM50 intrinsic subtype classification.

    Genomic Sequencing:

    Article Title: Tumor Transcriptome Sequencing Reveals Allelic Expression Imbalances Associated with Copy Number Alterations
    Article Snippet: .. aCGH was performed using the Agilent Human Genome Microarray Kit 244K (Agilent Technologies, Santa Clara, CA) which contains ∼244,000 60-mer oligonucleotide probes spanning coding and non-coding genomic sequences with median spacing of 7.4 and 16.5 kb respectively. .. Arrays were analyzed using the Genepix 4200A scanner (Axon Instruments, Union City, CA) and the Agilent Feature Extraction software (v9.1).



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    ( A ) The MEIS1 promoter is hypermethylated in colorectal tumors with a BRAF p.V600E mutation (black dots) when compared to wild type BRAF (white dots). The Y-axis represents the tumor vs. normal log 2 ratio for the median probe per <t>CpG</t> fragment. The horizontal dotted line at log 2 ratio 0 indicates an equal extent of MEIS1 methylation in tumor and normal samples. ( B ) Overview of the analyzed MEIS1 promoter, <t>CpG</t> <t>islands</t> within the promoter and the locus analyzed by MSP primers. Locations were based on the human genome browser (UCSC assembly March 2006, hg18). ( C ) MEIS1 -MSP data showing hypermethylation in BRAF p.V600E colorectal tumors when compared to BRAF wild types. T: tumor; N: normal tissue; M: methylated MEIS1 promoter (168 bp); Um: Unmethylated MEIS1 promoter (176 bp).
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    Genomic and transcriptomic evaluation of breast tumor patient-derived xenografts

    Journal: Breast Cancer Research : BCR

    Article Title: Patient-derived breast tumor xenografts facilitating personalized cancer therapy

    doi: 10.1186/bcr3355

    Figure Lengend Snippet: Genomic and transcriptomic evaluation of breast tumor patient-derived xenografts

    Article Snippet: Genomic , DNA copy number alterations: 14/18 pairs of tumors shared more than 56% copy number alterations, unsupervised hierarchical clustering showed 16/18 pairs segregated together. Recurrent changes between patient tumors and xenografts showed losses in 176 chromosomal regions and gains in 202 chromosomal regions , CGH array Agilent 244K human genome CGH microarrays. CGH results showed shared alterations between primary and xenograft tumors with more pronounced alterations in engrafted tumors, that is, TP53 patient 1 wild-type allele, xenograft LOH; ER+ tumor gained basal-like alterations , Paired-end sequencing to achieve deep coverage of patient blood, tumor, metastasis, and xenografted tumor Confirmed SNP coverage by Illumina 1M duo arrays. The PDX retained primary tumor mutations and showed enrichment of mutations similar to patient metastasis , Genome-wide SNPs with enhancement of existing aberrations , , .

    Techniques: Sequencing, Genome Wide, Microarray, Expressing

    ( A ) The MEIS1 promoter is hypermethylated in colorectal tumors with a BRAF p.V600E mutation (black dots) when compared to wild type BRAF (white dots). The Y-axis represents the tumor vs. normal log 2 ratio for the median probe per CpG fragment. The horizontal dotted line at log 2 ratio 0 indicates an equal extent of MEIS1 methylation in tumor and normal samples. ( B ) Overview of the analyzed MEIS1 promoter, CpG islands within the promoter and the locus analyzed by MSP primers. Locations were based on the human genome browser (UCSC assembly March 2006, hg18). ( C ) MEIS1 -MSP data showing hypermethylation in BRAF p.V600E colorectal tumors when compared to BRAF wild types. T: tumor; N: normal tissue; M: methylated MEIS1 promoter (168 bp); Um: Unmethylated MEIS1 promoter (176 bp).

    Journal: PLoS ONE

    Article Title: The Homeobox Gene MEIS1 Is Methylated in BRAF p.V600E Mutated Colon Tumors

    doi: 10.1371/journal.pone.0079898

    Figure Lengend Snippet: ( A ) The MEIS1 promoter is hypermethylated in colorectal tumors with a BRAF p.V600E mutation (black dots) when compared to wild type BRAF (white dots). The Y-axis represents the tumor vs. normal log 2 ratio for the median probe per CpG fragment. The horizontal dotted line at log 2 ratio 0 indicates an equal extent of MEIS1 methylation in tumor and normal samples. ( B ) Overview of the analyzed MEIS1 promoter, CpG islands within the promoter and the locus analyzed by MSP primers. Locations were based on the human genome browser (UCSC assembly March 2006, hg18). ( C ) MEIS1 -MSP data showing hypermethylation in BRAF p.V600E colorectal tumors when compared to BRAF wild types. T: tumor; N: normal tissue; M: methylated MEIS1 promoter (168 bp); Um: Unmethylated MEIS1 promoter (176 bp).

    Article Snippet: DNA from the 19 colorectal tumors were hybridized on Agilent 244k human CpG island microarrays (Agilent Technologies, Santa Clara, CA, U.S.A.), as described previously [ ].

    Techniques: Mutagenesis, Methylation