1xdapi (MedChemExpress)
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MedChemExpress
1xdapi
1xdapi, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1xdapi/DAPI/pmc12925565-287-25-26
Average 97 stars, based on 121 article reviews
1xdapi, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1xdapi/DAPI/pmc12925565-287-25-26
Average 97 stars, based on 121 article reviews
1xdapi - by Bioz Stars,
2026-10
97/100 stars
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Staining:Article Title: Foxn1 regulates epidermal structure, redox balance, and age related changes Article Snippet: Slides were washed three times with TBS-Tween (TBS-T) and incubated with fluorescent secondary antibody Alexa Fluor 488 (1:1000, Cat# A-11073, Invitrogen), Alexa Fluor 647 (1:1000, Cat# A-11032, Invitrogen) and Alexa Fluor 594 (1:1000, Cat# A-11037) for 1 h at room temperature. .. Afterward, selected sections were stained with WGA dye for 15 min then all Article Title: Environmental co-exposure to melamine and cyanuric acid induces ferroptotic kidney injury and fibrosis via HIF-1α/HO-1 signaling Article Snippet: Subsequently, the slides were incubated with primary antibodies against TGF-β (dilution 1:100; 346,599, Zenbio, China) and Smad2 (HY-P80324, MCE, China). .. Then, the slides were incubated with Cy3 (dilution 1:200; HY-P81017, MCE, China) for 1 hour and stained with Article Title: Bismuth-coordinated nanocomplex drives M2 macrophage polarization via mitochondrial reprogramming against invasive aspergillosis Article Snippet: Bi@CBD-DFO nanocomplex was mixed with the near-infrared fluorescent dye DiR (HY-D1048, MCE) at a mass ratio of 10:1 in PBS (pH 7.4) with stirring at room temperature in the dark for 2 h, and free DiR was removed by ultrafiltration to obtain DiR-Bi@CBD-DFO. .. RAW264.7 macrophages were seeded in confocal dishes, and heatinactivated A. fumigatus spores (MOI = 2) and DiR-Bi@CBD-DFO (10 μg/mL) were added, followed by incubation for 4, 12, 24, and 36 h. After fixation, nuclei were stained with Whole Genome Amplification:Article Title: Foxn1 regulates epidermal structure, redox balance, and age related changes Article Snippet: Slides were washed three times with TBS-Tween (TBS-T) and incubated with fluorescent secondary antibody Alexa Fluor 488 (1:1000, Cat# A-11073, Invitrogen), Alexa Fluor 647 (1:1000, Cat# A-11032, Invitrogen) and Alexa Fluor 594 (1:1000, Cat# A-11037) for 1 h at room temperature. .. Afterward, selected sections were stained with WGA dye for 15 min then all Incubation:Article Title: Environmental co-exposure to melamine and cyanuric acid induces ferroptotic kidney injury and fibrosis via HIF-1α/HO-1 signaling Article Snippet: Subsequently, the slides were incubated with primary antibodies against TGF-β (dilution 1:100; 346,599, Zenbio, China) and Smad2 (HY-P80324, MCE, China). .. Then, the slides were incubated with Cy3 (dilution 1:200; HY-P81017, MCE, China) for 1 hour and stained with Article Title: Bismuth-coordinated nanocomplex drives M2 macrophage polarization via mitochondrial reprogramming against invasive aspergillosis Article Snippet: Bi@CBD-DFO nanocomplex was mixed with the near-infrared fluorescent dye DiR (HY-D1048, MCE) at a mass ratio of 10:1 in PBS (pH 7.4) with stirring at room temperature in the dark for 2 h, and free DiR was removed by ultrafiltration to obtain DiR-Bi@CBD-DFO. .. RAW264.7 macrophages were seeded in confocal dishes, and heatinactivated A. fumigatus spores (MOI = 2) and DiR-Bi@CBD-DFO (10 μg/mL) were added, followed by incubation for 4, 12, 24, and 36 h. After fixation, nuclei were stained with Confocal:Article Title: Bismuth-coordinated nanocomplex drives M2 macrophage polarization via mitochondrial reprogramming against invasive aspergillosis Article Snippet: Bi@CBD-DFO nanocomplex was mixed with the near-infrared fluorescent dye DiR (HY-D1048, MCE) at a mass ratio of 10:1 in PBS (pH 7.4) with stirring at room temperature in the dark for 2 h, and free DiR was removed by ultrafiltration to obtain DiR-Bi@CBD-DFO. .. RAW264.7 macrophages were seeded in confocal dishes, and heatinactivated A. fumigatus spores (MOI = 2) and DiR-Bi@CBD-DFO (10 μg/mL) were added, followed by incubation for 4, 12, 24, and 36 h. After fixation, nuclei were stained with Microscopy:Article Title: Bismuth-coordinated nanocomplex drives M2 macrophage polarization via mitochondrial reprogramming against invasive aspergillosis Article Snippet: Bi@CBD-DFO nanocomplex was mixed with the near-infrared fluorescent dye DiR (HY-D1048, MCE) at a mass ratio of 10:1 in PBS (pH 7.4) with stirring at room temperature in the dark for 2 h, and free DiR was removed by ultrafiltration to obtain DiR-Bi@CBD-DFO. .. RAW264.7 macrophages were seeded in confocal dishes, and heatinactivated A. fumigatus spores (MOI = 2) and DiR-Bi@CBD-DFO (10 μg/mL) were added, followed by incubation for 4, 12, 24, and 36 h. After fixation, nuclei were stained with |