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Abstract
We studied 60 clinically diagnosed, patch test-confirmed patients with Parthenium dermatitis who were eligible for azathioprine treatment. Patch testing with an aqueous extract of the plant antigen was performed before initiation of azathioprine treatment. The extract was prepared by using 50 g of the dried leaf powder of the plant mixed with 300 ml of distilled water, and kept at room temperature for 24 h. The mixture was then filtered through
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