Journal: PLoS Genetics
Article Title: Transcript Expression Data from Human Islets Links Regulatory Signals from Genome-Wide Association Studies for Type 2 Diabetes and Glycemic Traits to Their Downstream Effectors
Figure Lengend Snippet: Islet eQTL data identifies ZMIZ1 as a novel gene involved in maintenance of glucose homeostasis in the human islet. (a) Regional plot showing the T2D-associated variant rs12571751 is in strong LD with the lead eQTL variant for ZMIZ1, and overlaps a long stretch of islet enhancer chromatin (denoted as red and blue in the tracks underneath the plot). (b) Immunofluorescence shows ZMIZ1 localizes to the islet within human pancreas sections, with staining in both alpha- and beta-cells. Effect of ZMIZ1 over-expression (c) and knockdown (d) on insulin secretion in human islets, showing significant (p
Article Snippet: Cells were plated on 35mm dishes and transfected with control (pEGFP-N1, Clontech, Mountain View, CA, USA) or ZMIZ1 over-expression (ZMIZ1 pCMV6- AC-GFP, Origene, Rockville, MD, USA) plasmids via lipid transfection (Lipofectamine 2000, Life Technologies, Canada).
Techniques: Variant Assay, Immunofluorescence, Staining, Over Expression