zic3 shrna sequences Search Results


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    Thermo Fisher zic3 shrna sequences
    <t>shRNA-mediated</t> <t>Zic3</t> silencing reduces generation of iPSCs. (A) Endogenous Zic3 was efficiently silenced by shRNA in mESC as demonstrated by qRT-PCR. (B) Nanog expression was analyzed by qRT-PCR 0, 5, 10, and 15 days post-transduction of an anti-Zic3 shRNA
    Zic3 Shrna Sequences, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    zic3 shrna sequences - by Bioz Stars, 2020-04
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    shRNA-mediated Zic3 silencing reduces generation of iPSCs. (A) Endogenous Zic3 was efficiently silenced by shRNA in mESC as demonstrated by qRT-PCR. (B) Nanog expression was analyzed by qRT-PCR 0, 5, 10, and 15 days post-transduction of an anti-Zic3 shRNA

    Journal: Stem Cells and Development

    Article Title: Zic3 Enhances the Generation of Mouse Induced Pluripotent Stem Cells

    doi: 10.1089/scd.2012.0651

    Figure Lengend Snippet: shRNA-mediated Zic3 silencing reduces generation of iPSCs. (A) Endogenous Zic3 was efficiently silenced by shRNA in mESC as demonstrated by qRT-PCR. (B) Nanog expression was analyzed by qRT-PCR 0, 5, 10, and 15 days post-transduction of an anti-Zic3 shRNA

    Article Snippet: In brief, Zic3 ShRNA sequences obtained from Open biosystems (V3THS_304236, V3THS_304235) were cloned in the pTRIPZ doxycycline inducible lentiviral vector by digesting with Eco RI and Xho I and transfected into 293T cells using viral packaging plasmids pMD2.G and psPAX2 (Addgene).

    Techniques: shRNA, Quantitative RT-PCR, Expressing, Transduction