yoyo-1 Thermo Fisher Search Results


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  • 95
    Thermo Fisher yoyo 1 iodide thermo fisher
    Yoyo 1 Iodide Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher yoyo 1
    Fluorescent image of <t>YOYO-stained,</t> device-extracted DNA, stretched on silanized glass in 100 µm wide and 5 µm deep PDMS channel. The image is stitched to cover 8 ROIs of an Andor Zyla camera at 100x magnification. The 32 µm scale bar corresponds to 100 kbp.
    Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher yoyo 1 iodide
    (A). In vitro cellular uptake of <t>YOYO-1</t> labeled DNA delivered by IP and SP. Data are shown as mean ± SD (n=3). (B). Confocal images of HeLa cells treated with polyplexes containing YOYO-1 labeled DNA for 4 h and stained with LysoTracker Red. DAPI (4′,6-diamidino-2-phenylindole, blue) was used to stain cell nuclei. Scale bar: 10 μm. (C). Colocalization ratio of YOYO-1-DNA with LysoTracker Red stained endosomes. Results were presented as the mean of 15 individual cells. Data are shown as mean ± SD (n=15; student's t test, *p
    Yoyo 1 Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 intercalator
    (A). In vitro cellular uptake of <t>YOYO-1</t> labeled DNA delivered by IP and SP. Data are shown as mean ± SD (n=3). (B). Confocal images of HeLa cells treated with polyplexes containing YOYO-1 labeled DNA for 4 h and stained with LysoTracker Red. DAPI (4′,6-diamidino-2-phenylindole, blue) was used to stain cell nuclei. Scale bar: 10 μm. (C). Colocalization ratio of YOYO-1-DNA with LysoTracker Red stained endosomes. Results were presented as the mean of 15 individual cells. Data are shown as mean ± SD (n=15; student's t test, *p
    Yoyo 1 Intercalator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 dye
    (a) Schematic of possible outcomes of DNA concatemer formation with 5-cytosine methylated (5mC) and non-methylated segments. (b) Schematic of Alexa568-MBD to DNA concatemer. The entire molecule is stained using the green stain <t>YOYO-1</t> and Alexa568-MBD binds to methylated stretches.
    Yoyo 1 Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher nℳ yoyo 1
    (a) Schematic of possible outcomes of DNA concatemer formation with 5-cytosine methylated (5mC) and non-methylated segments. (b) Schematic of Alexa568-MBD to DNA concatemer. The entire molecule is stained using the green stain <t>YOYO-1</t> and Alexa568-MBD binds to methylated stretches.
    Nℳ Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 fluorochrome
    (a) Schematic of possible outcomes of DNA concatemer formation with 5-cytosine methylated (5mC) and non-methylated segments. (b) Schematic of Alexa568-MBD to DNA concatemer. The entire molecule is stained using the green stain <t>YOYO-1</t> and Alexa568-MBD binds to methylated stretches.
    Yoyo 1 Fluorochrome, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher yoyo 1 fluorescent dye
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Yoyo 1 Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 nuclear dye
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Yoyo 1 Nuclear Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher stock yoyo 1 dye
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Stock Yoyo 1 Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 iodide dye
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Yoyo 1 Iodide Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher intercalating fluorescence dye yoyo 1
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Intercalating Fluorescence Dye Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher oxasole yellow yoyo 1 iodide
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Oxasole Yellow Yoyo 1 Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dimeric cyanine dye yoyo 1
    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of <t>YOYO-1</t> dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading
    Dimeric Cyanine Dye Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dna dye yoyo 1
    Safranin O staining of aggregates (A) and immunostaining of type II collagen in aggregates (B, top ) of human adipose stem cells with (GDF5) or without (non-GDF5) infection of ad-GDF5 and cultured in BM or CM for 3 weeks. Counterstaining was performed with a <t>DNA</t> fluorescent dye <t>YOYO-1</t> (B, bottom ) . The negative control group was the non-GDF5/CM group without incubation of the primary antibody. Bar=100 μm.
    Dna Dye Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bis intercalating dye yoyo 1 iodide
    <t>YOYO-1</t> fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.
    Bis Intercalating Dye Yoyo 1 Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher yoyo 1 labeled dna
    <t>YOYO-1</t> fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.
    Yoyo 1 Labeled Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher yoyo 1 iodide509
    <t>YOYO-1</t> fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.
    Yoyo 1 Iodide509, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bis intercalating fluorescent dye yoyo 1
    <t>YOYO-1</t> fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.
    Bis Intercalating Fluorescent Dye Yoyo 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher yoyo 1 fluorescent nucleic acid dye
    (A) Electropherograms for VSV separated by capillary electrophoresis and detected by laser-induced fluorescence. VSV was stained with <t>YOYO-1</t> dye. CE separations were performed in a 60 cm long capillary under 250 V cm – 1 in 25 mM borax buffer at 15 °C. (i) Fresh, not frozen VSV; (ii–v) VSV after 1 cycle of freeze–thaw, untreated and treated with the library, an aptamer, and a quadramer, respectively. (B) Areas of intact virus peaks for all experiments.
    Yoyo 1 Fluorescent Nucleic Acid Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fluorescent image of YOYO-stained, device-extracted DNA, stretched on silanized glass in 100 µm wide and 5 µm deep PDMS channel. The image is stitched to cover 8 ROIs of an Andor Zyla camera at 100x magnification. The 32 µm scale bar corresponds to 100 kbp.

    Journal: Lab on a chip

    Article Title: Microfluidic long DNA sample preparation from cells

    doi: 10.1039/c8lc01163j

    Figure Lengend Snippet: Fluorescent image of YOYO-stained, device-extracted DNA, stretched on silanized glass in 100 µm wide and 5 µm deep PDMS channel. The image is stitched to cover 8 ROIs of an Andor Zyla camera at 100x magnification. The 32 µm scale bar corresponds to 100 kbp.

    Article Snippet: Cells were lysed with a lysis solution containing 70 µL RIPA buffer (Thermo Fisher Scientific; 0.1% SDS), 10 µL pH 8 TE buffer (Thermo Fisher Scientific), 10 µL SDS lysis buffer (Sigma; 1% SDS), Proteinase K (Qiagen) at a concentration of 2 mg/mL, and YOYO-1 (Thermo Fisher Scientific) at a concentration of 4 µM.

    Techniques: Staining

    (A). In vitro cellular uptake of YOYO-1 labeled DNA delivered by IP and SP. Data are shown as mean ± SD (n=3). (B). Confocal images of HeLa cells treated with polyplexes containing YOYO-1 labeled DNA for 4 h and stained with LysoTracker Red. DAPI (4′,6-diamidino-2-phenylindole, blue) was used to stain cell nuclei. Scale bar: 10 μm. (C). Colocalization ratio of YOYO-1-DNA with LysoTracker Red stained endosomes. Results were presented as the mean of 15 individual cells. Data are shown as mean ± SD (n=15; student's t test, *p

    Journal: Biomaterials

    Article Title: Development of switchable polymers to address the dilemma of stability and cargo release in polycationic nucleic acid carriers

    doi: 10.1016/j.biomaterials.2017.02.036

    Figure Lengend Snippet: (A). In vitro cellular uptake of YOYO-1 labeled DNA delivered by IP and SP. Data are shown as mean ± SD (n=3). (B). Confocal images of HeLa cells treated with polyplexes containing YOYO-1 labeled DNA for 4 h and stained with LysoTracker Red. DAPI (4′,6-diamidino-2-phenylindole, blue) was used to stain cell nuclei. Scale bar: 10 μm. (C). Colocalization ratio of YOYO-1-DNA with LysoTracker Red stained endosomes. Results were presented as the mean of 15 individual cells. Data are shown as mean ± SD (n=15; student's t test, *p

    Article Snippet: YOYO-1 iodide was purchased from Invitrogen (Carlsbad, CA).

    Techniques: In Vitro, Labeling, Staining

    (a) Schematic of possible outcomes of DNA concatemer formation with 5-cytosine methylated (5mC) and non-methylated segments. (b) Schematic of Alexa568-MBD to DNA concatemer. The entire molecule is stained using the green stain YOYO-1 and Alexa568-MBD binds to methylated stretches.

    Journal: Biomicrofluidics

    Article Title: DNA methylation profiling in nanochannels

    doi: 10.1063/1.3613671

    Figure Lengend Snippet: (a) Schematic of possible outcomes of DNA concatemer formation with 5-cytosine methylated (5mC) and non-methylated segments. (b) Schematic of Alexa568-MBD to DNA concatemer. The entire molecule is stained using the green stain YOYO-1 and Alexa568-MBD binds to methylated stretches.

    Article Snippet: Alexa568 labeled DNA is counter stained with the intercalating dye YOYO-1 (Invitrogen) at a ratio of 1 dye molecule per 50 base pairs of DNA.

    Techniques: Methylation, Staining

    (a) Fluorescence images of concatenated methylated and non-methylated λ-DNA labeled with Alexa568MBD (red) and YOYO-1 (green), stretched out in nanochannels. Within each panel colors are split for clarity; (left) YOYO-1 only (DNA), (center) composite, (right) Alexa568 only (Alexa568-MBD). Schematic drawings in each panel illustrate the spatial position of the Alexa Fluor 568 MBD and the length of the λ-DNA. The scale bar in panel (b) is 5 microns.

    Journal: Biomicrofluidics

    Article Title: DNA methylation profiling in nanochannels

    doi: 10.1063/1.3613671

    Figure Lengend Snippet: (a) Fluorescence images of concatenated methylated and non-methylated λ-DNA labeled with Alexa568MBD (red) and YOYO-1 (green), stretched out in nanochannels. Within each panel colors are split for clarity; (left) YOYO-1 only (DNA), (center) composite, (right) Alexa568 only (Alexa568-MBD). Schematic drawings in each panel illustrate the spatial position of the Alexa Fluor 568 MBD and the length of the λ-DNA. The scale bar in panel (b) is 5 microns.

    Article Snippet: Alexa568 labeled DNA is counter stained with the intercalating dye YOYO-1 (Invitrogen) at a ratio of 1 dye molecule per 50 base pairs of DNA.

    Techniques: Fluorescence, Methylation, Labeling

    Single-channel LFC. ( a ) Single-channel LFC mounted on the microscope stage and connected to the pumping system. ( b ) The 3D design of the single-channel LFC. ( c ) The depiction of immobilization and linearization of a single DNA molecule (green line) interacting with a fluorescently labelled protein (red circle). The DNA is held in place using an optical trap (red cone) to control a polystyrene microsphere (black circle) attached to one end of the DNA. ( d ) Bright-field microscope image of the trapped polystyrene microsphere (top; position of DNA indicated with dashed line), pseudo-coloured fluorescent image of DNA labelled with YOYO (middle), pseudo-coloured fluorescent image of a single protein molecule labelled with ATTO647N while scanning along the DNA with gamma adjusted to 1.3 (bottom). White scale bars equal 1 µm. ( e ) A kymograph of the movement of protein along DNA with a duration of around 2 seconds; the horizontal and vertical white scale bars equal 1 µm and 200 ms, respectively. ( f ) Density distribution of proteins’ movement within frame intervals of 13.5 m for a collection of 49 trajectories of AlkF as exemplified in ( e ). ( g ) Mean squared displacement (MSD) of 49 scanning trajectories of AlkF along DNA. Error bars represent standard error of the mean (SEM).

    Journal: Scientific Reports

    Article Title: Additive manufacturing of laminar flow cells for single-molecule experiments

    doi: 10.1038/s41598-019-53151-z

    Figure Lengend Snippet: Single-channel LFC. ( a ) Single-channel LFC mounted on the microscope stage and connected to the pumping system. ( b ) The 3D design of the single-channel LFC. ( c ) The depiction of immobilization and linearization of a single DNA molecule (green line) interacting with a fluorescently labelled protein (red circle). The DNA is held in place using an optical trap (red cone) to control a polystyrene microsphere (black circle) attached to one end of the DNA. ( d ) Bright-field microscope image of the trapped polystyrene microsphere (top; position of DNA indicated with dashed line), pseudo-coloured fluorescent image of DNA labelled with YOYO (middle), pseudo-coloured fluorescent image of a single protein molecule labelled with ATTO647N while scanning along the DNA with gamma adjusted to 1.3 (bottom). White scale bars equal 1 µm. ( e ) A kymograph of the movement of protein along DNA with a duration of around 2 seconds; the horizontal and vertical white scale bars equal 1 µm and 200 ms, respectively. ( f ) Density distribution of proteins’ movement within frame intervals of 13.5 m for a collection of 49 trajectories of AlkF as exemplified in ( e ). ( g ) Mean squared displacement (MSD) of 49 scanning trajectories of AlkF along DNA. Error bars represent standard error of the mean (SEM).

    Article Snippet: The other reservoir was filled with YOYO-1 intercalating dye (ThermoFisher Scientific) dissolved in a ROXS buffer consisting of 400 μl of 10% w/v glucose, 34 μl of 60 mM ascorbic acid (Fluka), 34 μl of 98% 60 mM methylviologen hydrat (Sigma-Aldrich), 10 μl of 1 M TRIS at pH 8, 2 μl of 10 μM YOYO-1, 50 μl of enzyme stock solution and 480 μl double-distilled water.

    Techniques: Microscopy, Mass Spectrometry

    DNA dumbbell construction. Step-by-step process of DNA dumbbell construction and exposure to intercalating dye in a flow-free environment using reservoir-based LFC. (1) Individual trapping of DNA-attached streptavidin coated (diameter: 1.76 µm) and free anti-digoxigenin coated beads (diameter: 0.9 µm) within the reservoir and translocating into the main channel; (top-right inset: bright-field image of the trapped beads) (2) applying gentle flow (0.05 mm/s) for a few seconds to elongate DNA and attachment to second bead (3) Incubation of the DNA dumbbell with YOYO-1 in a flow-free reservoir. (4) Visualization of the DNA in the flow-free main channel; bottom-left inset: fluorescent image of DNA dumbbell.

    Journal: Scientific Reports

    Article Title: Additive manufacturing of laminar flow cells for single-molecule experiments

    doi: 10.1038/s41598-019-53151-z

    Figure Lengend Snippet: DNA dumbbell construction. Step-by-step process of DNA dumbbell construction and exposure to intercalating dye in a flow-free environment using reservoir-based LFC. (1) Individual trapping of DNA-attached streptavidin coated (diameter: 1.76 µm) and free anti-digoxigenin coated beads (diameter: 0.9 µm) within the reservoir and translocating into the main channel; (top-right inset: bright-field image of the trapped beads) (2) applying gentle flow (0.05 mm/s) for a few seconds to elongate DNA and attachment to second bead (3) Incubation of the DNA dumbbell with YOYO-1 in a flow-free reservoir. (4) Visualization of the DNA in the flow-free main channel; bottom-left inset: fluorescent image of DNA dumbbell.

    Article Snippet: The other reservoir was filled with YOYO-1 intercalating dye (ThermoFisher Scientific) dissolved in a ROXS buffer consisting of 400 μl of 10% w/v glucose, 34 μl of 60 mM ascorbic acid (Fluka), 34 μl of 98% 60 mM methylviologen hydrat (Sigma-Aldrich), 10 μl of 1 M TRIS at pH 8, 2 μl of 10 μM YOYO-1, 50 μl of enzyme stock solution and 480 μl double-distilled water.

    Techniques: Flow Cytometry, Incubation

    Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of YOYO-1 dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Convex lens-induced nanoscale templating

    doi: 10.1073/pnas.1321089111

    Figure Lengend Snippet: Denaturation mapping of λ-DNA in 50-nm channels. ( A ) Kymograph showing initial loading of DNA into a nanochannels and release of YOYO-1 dye to reveal the characteristic λ-DNA melting profile. ( B ) Kymograph taken ∼1 min after loading

    Article Snippet: The DNA was stained with YOYO-1 fluorescent dye (Life Technologies) at a ratio of one dye molecule per 10 bp.

    Techniques: Denaturation Mapping

    Safranin O staining of aggregates (A) and immunostaining of type II collagen in aggregates (B, top ) of human adipose stem cells with (GDF5) or without (non-GDF5) infection of ad-GDF5 and cultured in BM or CM for 3 weeks. Counterstaining was performed with a DNA fluorescent dye YOYO-1 (B, bottom ) . The negative control group was the non-GDF5/CM group without incubation of the primary antibody. Bar=100 μm.

    Journal: BioResearch Open Access

    Article Title: A Modified Aggregate Culture for Chondrogenesis of Human Adipose-Derived Stem Cells Genetically Modified with Growth and Differentiation Factor 5

    doi: 10.1089/biores.2013.0014

    Figure Lengend Snippet: Safranin O staining of aggregates (A) and immunostaining of type II collagen in aggregates (B, top ) of human adipose stem cells with (GDF5) or without (non-GDF5) infection of ad-GDF5 and cultured in BM or CM for 3 weeks. Counterstaining was performed with a DNA fluorescent dye YOYO-1 (B, bottom ) . The negative control group was the non-GDF5/CM group without incubation of the primary antibody. Bar=100 μm.

    Article Snippet: After color development with the mouse immunoCruz system, cells were counterstained for 10 min at room temperature with a fluorescent DNA dye YOYO-1 (Life Technologies, Grand Island, NY).

    Techniques: Staining, Immunostaining, Infection, Cell Culture, Negative Control, Incubation

    YOYO-1 fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.

    Journal: Biomaterials

    Article Title: Optimization of Tet1 ligand density in HPMA-co-oligolysine copolymers for targeted neuronal gene delivery

    doi: 10.1016/j.biomaterials.2013.08.045

    Figure Lengend Snippet: YOYO-1 fluorescence quenching assay as a measure of DNA complexation and condensation in ddH 2 O.

    Article Snippet: pCMV-Luc2 plasmid was mixed with the bis-intercalating dye YOYO-1 iodide (Invitrogen, Carlsbad, CA) at a dye/base pair ratio of 1:50 and incubated at room temperature for 1 h. Polyplexes were formed at N/P ratios of 0, 1, 2, 4, 6, and 10 by complexing YOYO-1-labeled DNA with pHT1K10, pHT3K10, pHT5K10, or pHK10.

    Techniques: Fluorescence

    (A) Electropherograms for VSV separated by capillary electrophoresis and detected by laser-induced fluorescence. VSV was stained with YOYO-1 dye. CE separations were performed in a 60 cm long capillary under 250 V cm – 1 in 25 mM borax buffer at 15 °C. (i) Fresh, not frozen VSV; (ii–v) VSV after 1 cycle of freeze–thaw, untreated and treated with the library, an aptamer, and a quadramer, respectively. (B) Areas of intact virus peaks for all experiments.

    Journal: ACS Medicinal Chemistry Letters

    Article Title: Aptamer-Facilitated Cryoprotection of Viruses

    doi: 10.1021/ml500322h

    Figure Lengend Snippet: (A) Electropherograms for VSV separated by capillary electrophoresis and detected by laser-induced fluorescence. VSV was stained with YOYO-1 dye. CE separations were performed in a 60 cm long capillary under 250 V cm – 1 in 25 mM borax buffer at 15 °C. (i) Fresh, not frozen VSV; (ii–v) VSV after 1 cycle of freeze–thaw, untreated and treated with the library, an aptamer, and a quadramer, respectively. (B) Areas of intact virus peaks for all experiments.

    Article Snippet: Prior to separating each sample by capillary electrophoresis, they were stained with 2.0 μM YOYO-1 fluorescent nucleic acid dye (Invitrogen, CA).

    Techniques: Electrophoresis, Fluorescence, Staining