Journal: International Journal of Molecular Sciences

Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus

doi: 10.3390/ijms15010944

Figure Lengend Snippet: Michaelis-Menten and Lineweaver-Burk plots of RNase activity. The K m value was estimated to be 169 with a V max of 0.019. The acid soluble assay at 55 °C with varying RNA substrate concentrations was used to determine the velocities. The data was then converted to generate the Lineweaver-Burk plot.

Article Snippet: A modified version of the acid soluble assay was used which involved adding 0.75 mL of partially purified RNase to 0.75 mL of substrate (yeast RNA; 1 mg/mL (Sigma Cat# R6625; Milwaukee, WI, USA), 10 μmol MgSO4 in a 0.1 M imidazole, malic acid, and glycine buffer, at various pH) and allowed to incubate at 55 °C for 10 min.

Techniques: Activity Assay

Journal: Life

Article Title: Amphiphilic Compounds Assemble into Membranous Vesicles in Hydrothermal Hot Spring Water but Not in Seawater

doi: 10.3390/life8020011

Figure Lengend Snippet: Lauric acid–glycerol monolaurate (LA–GML, 10 mM) was mixed with yeast ribosomal RNA in a 2:1 ratio by weight in pH 3.3 Geyser Basin water, then dried on a microscope and rehydrated with the same volume of water. Phase ( A ) and fluorescence ( B ) images. ( C ) A control in which the same lipid mixture was prepared without a wet–dry cycle and put through the gel permeation column in the absence of RNA.

Article Snippet: To test encapsulation of nucleic acids, LA–GML was mixed in a 2:1 ratio by weight with either Torula yeast ribosomal RNA (CHEM-IMPEX Int’l Inc. Wood Dale, IL) or with lambda phage DNA.

Techniques: Microscopy, Fluorescence