Journal: Molecular Plant
Article Title: Hetero-trans-β-Glucanase Produces Cellulose–Xyloglucan Covalent Bonds in the Cell Walls of Structural Plant Tissues and Is Stimulated by Expansin
Figure Lengend Snippet: Thin-Layer Chromatographic Profiling Diagnostic Fingerprints of In Vitro- Formed XET, MXE, and CXE Products. Polymer–[ 3 H]XXXGol conjugates, prepared enzymically in vitro , were digested with commercial enzymes and the products analyzed by thin-layer chromatography. Circled letters C, L, and X with heavy arrows indicate the expected sites of attack by xyloglucan-inactive cellulase, lichenase, and xyloglucan endoglucanase, respectively. Circled letters with faint arrows indicate unexpected sites of (slight) attack, possibly due to contaminating enzymes. (A) Markers were [ 3 H]XXXGol and [ 3 H]isoprimeveritol (IP-ol). Other profiles show the products formed from: the CXE product, cellulose–[ 3 H]XXXGol (B and C) ; the MXE product, MLG–[ 3 H]XXXGol (D–F) ; and the XET product, xyloglucan–[ 3 H]XXXGol (G–I) —by lichenase (D and G) , xyloglucan endoglucanase (B, E, and H) , or xyloglucan-inactive cellulase (C, F, and I) . On the structural diagrams, circle denotes glucose residue, star denotes xylose residue, and zigzag denotes [ 3 H]glucitol.
Article Snippet: Xyloglucan endoglucanase was a gift from Novo Nordisk (Bagsværd, Denmark; ).
Techniques: Diagnostic Assay, In Vitro, Thin Layer Chromatography