xmai Takara Search Results


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  • 99
    New England Biolabs xmai
    Xmai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1079 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa ecori xmai blunted peyfp n1
    Ecori Xmai Blunted Peyfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa xmai sali pgbt9
    Xmai Sali Pgbt9, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa ecori xmai
    Ecori Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa xmai mlui cut pegfp c1
    Xmai Mlui Cut Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    xmai  (TaKaRa)
    95
    TaKaRa xmai
    Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa xmai saci cleaved pact2
    Xmai Saci Cleaved Pact2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa pacgfp1 1
    Pacgfp1 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa egfp c1
    Egfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    xbai  (TaKaRa)
    99
    TaKaRa xbai
    Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (TaKaRa)
    95
    TaKaRa spei
    Spei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa mightymix ligation
    Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa pgad424
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Pgad424, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 2096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa peyfp c1
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Peyfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa sacii
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Sacii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa pegfp
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Pegfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 4959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    xhoi  (TaKaRa)
    99
    TaKaRa xhoi
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa ecori
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Ecori, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 8219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa plasmid pcmvβ
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Plasmid Pcmvβ, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pgadt7 leu2 vector
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Pgadt7 Leu2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa pegfp n1 plasmid
    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid <t>pGBKT7</t> (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).
    Pegfp N1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid pGBKT7 (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).

    Journal: Nucleic Acids Research

    Article Title: Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes

    doi: 10.1093/nar/gkt1293

    Figure Lengend Snippet: Directed yeast two-hybrid assays. Yeast strain AH109 was transformed with bait plasmid pGBKT7 (Vec) or its derivative pGBK-DBP4 or PGBK-ENP2, and prey plasmid pGADT7 (Vec) or its derivatives pGAD-DBP4, pGAD-ENP2, pGAD-BFR2 and pGAD-NOP6. The bait and prey plasmids, respectively, carry TRP1 and LEU2 auxotrophic markers that allow growth on medium lacking tryptophan and leucine (upper panel). Interactions between bait and prey hybrid proteins activate transcription of the HIS3 reporter gene, which is monitored by growth on medium lacking histidine; addition of 2 or 20 mM 3-AT to this medium enhances the stringency of the HIS3 reporter, allowing detection of the strongest two-hybrid interactions (middle and lower panels, respectively).

    Article Snippet: Primers DBP4–forNco 5′-CAT GCC ATG GCC AAA AAA AAT AGA TTG AAC-3′ and DBP4–revXma 5′-CCC CCC GGG TTA ACC CTG GAT TAA TTT AGC TGT C-3′ were used, and the DNA fragment was cloned between the NcoI and XmaI sites of pGBKT7 (Clontech) to produce pGBK-DBP4.

    Techniques: Transformation Assay, Plasmid Preparation