xhoi Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs xhoi restriction sites underlined
    Effect of CD22ΔE12 vs. CD22 WT on clonogenicity and self-renewal rate of ALL cells. [A1] Depicted is a representative 1% agarose gel showing the PCR products of FL CD22 and CD22ΔE12–14. [A.2] Subcloning of the CD22 FL or CD22ΔE12–14 genes into the <t>lentiviral</t> pCL6-2AEGwo vector through NheI and <t>XhoI</t> restriction sites was confirmed through restriction enzyme digestion and DNA sequencing. Depicted is a representative 1% agarose gel showing that the generated lentiviral constructs have the correct size FL CD22 (2.5-kb) or CD22ΔE12–14 (2.2-kb) inserts. [B.1] In order to confirm that the lentiviral vectors can be used to achieve expression of FL and truncated CD22 in human cells, 293T cells were transfected with lentiviral constructs for FL CD22, CD22ΔE12–14, as well as pCL6-2AEGwo lentiviral vector without any subcloned CD22. 48 h post transfection cells were examined for FL CD22 and CD22ΔE12–14 mRNA by RT-PCR using the P7, P9, and P10 primer pairs. The P7 primer set was used to amplify a 182-bp region (c.2180–c.2361) of the CD22 cDNA extending from Exon 11 to Exon 13 and spanning the entire Exon 12. The P9 primer set was used amplify a 160-bp region (c.2304–c.2463) of the CD22 cDNA extending from Exon 12 to Exon 14 and spanning the entire Exon 13. The P10 primer set was used to amplify a 213-bp region (c.433–c.645) of Exon 4 of the CD22 cDNA present in both wildtype CD22 and CD22ΔE12–14 mRNA species. As expected all primer sets yielded PCR products in cells transfected with the FL CD22 vector and only the P10 primer set yielded a PCR product in cells transfected with the CD22ΔE12–14 vector. [B.2 and B3] The increased expression levels of the full-length and truncated proteins in transduced 293-T cells (depicted in B.2) and ALL-1 cells (depicted in B.3) were confirmed to be similar by Western blot analysis done at 96 h post-transduction. [C D] ALL cell lines DAUDI (Burkitt's leukemia/B-ALL) (panels C1 C.2) and ALL1 (BCR-ABL + B-precursor ALL) (panel C3) were transduced (trans) with wildtype and mutant human CD22 genes using the pCL6-2AEGwo lentiviral vector and then assayed for colony formation in semi-solid methylcellulose cultures without additional stroma support or cytokines. Colony formation was examined using an inverted Nikon Eclipse TS100 microscope with an Epifluorescence attachment. Images were taken using a Digital Sight DS-2MBW Nikon camera (System magnification: 100× or 200 × as indicated). Green fluorescence of colonies resulting from GFP expression confirms successful transduction of the cell lines. [D] Depicted are bar graphs comparing the mean colony numbers in cultures of untransduced and transduced cells.
    Xhoi Restriction Sites Underlined, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi restriction sites underlined/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xhoi restriction sites underlined - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Millipore xho i
    Schematic representation of the expression vector, pET32a-mBD2. The cDNA for mature mBD2 were amplified using <t>PCR</t> from the pcDNA3.1(+)-mBD2 plasmid. PCR product was cleaved by <t>Xho</t> I and Kpn I, and the mBD2 fragment was inserted into similarly digested pET32a(+) vector to construct the expression vector, pET32a-mBD2.
    Xho I, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xho i/product/Millipore
    Average 95 stars, based on 3010 article reviews
    Price from $9.99 to $1999.99
    xho i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher xho i
    (A) Digestion of pTY-α-amylase with <t>Nhe</t> I and <t>Xho</t> I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Xho I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4040 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xho i/product/Thermo Fisher
    Average 90 stars, based on 4040 article reviews
    Price from $9.99 to $1999.99
    xho i - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher fastdigest xhoi
    (A) Digestion of pTY-α-amylase with <t>Nhe</t> I and <t>Xho</t> I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Fastdigest Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest xhoi/product/Thermo Fisher
    Average 90 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    fastdigest xhoi - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    95
    Promega xhoi
    (A) Digestion of pTY-α-amylase with <t>Nhe</t> I and <t>Xho</t> I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Xhoi, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 5518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Promega
    Average 95 stars, based on 5518 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    xhoi  (Toyobo)
    90
    Toyobo xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Toyobo
    Average 90 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    xhoi  (Roche)
    93
    Roche xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Roche
    Average 93 stars, based on 484 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    89
    Boehringer Mannheim xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Boehringer Mannheim
    Average 89 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    93
    GE Healthcare xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/GE Healthcare
    Average 93 stars, based on 1056 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    89
    Fisher Scientific xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Fisher Scientific
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    93
    GenScript xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/GenScript
    Average 93 stars, based on 156 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    89
    Veritas Corporation xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Veritas Corporation, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Veritas Corporation
    Average 89 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    89
    PerkinElmer xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/PerkinElmer
    Average 89 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    89
    Stratagene xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 1008 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/Stratagene
    Average 89 stars, based on 1008 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    82
    Promega xhoi nuclease
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Nuclease, supplied by Promega, used in various techniques. Bioz Stars score: 82/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi nuclease/product/Promega
    Average 82 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    xhoi nuclease - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    75
    Roche ncoi xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Ncoi Xhoi, supplied by Roche, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncoi xhoi/product/Roche
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi xhoi - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    91
    Thermo Fisher ecori xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Ecori Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori xhoi/product/Thermo Fisher
    Average 91 stars, based on 173 article reviews
    Price from $9.99 to $1999.99
    ecori xhoi - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    78
    Thermo Fisher xhoi noti
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Noti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi noti/product/Thermo Fisher
    Average 78 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    xhoi noti - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    81
    TaKaRa hindiii xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Hindiii Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hindiii xhoi/product/TaKaRa
    Average 81 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hindiii xhoi - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    83
    TaKaRa quickcut xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Quickcut Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quickcut xhoi/product/TaKaRa
    Average 83 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    quickcut xhoi - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    96
    Thermo Fisher bamhi xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Bamhi Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi xhoi/product/Thermo Fisher
    Average 96 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    bamhi xhoi - by Bioz Stars, 2020-02
    96/100 stars
      Buy from Supplier

    78
    Thermo Fisher bglii xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Bglii Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii xhoi/product/Thermo Fisher
    Average 78 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    bglii xhoi - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    91
    TaKaRa xhoi endonucleases
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Endonucleases, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi endonucleases/product/TaKaRa
    Average 91 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    xhoi endonucleases - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    82
    TaKaRa xhoi xbai
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi xbai/product/TaKaRa
    Average 82 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xhoi xbai - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    87
    TaKaRa nhei xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Nhei Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei xhoi/product/TaKaRa
    Average 87 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    nhei xhoi - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    79
    Thermo Fisher xhoi hindiii
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi hindiii/product/Thermo Fisher
    Average 79 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    xhoi hindiii - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    85
    Promega kpni xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Kpni Xhoi, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni xhoi/product/Promega
    Average 85 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    kpni xhoi - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    77
    Roche xhoi ecori
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Xhoi Ecori, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi ecori/product/Roche
    Average 77 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xhoi ecori - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    85
    TaKaRa ecori xhoi
    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, <t>SpeI;</t> lane 16, <t>XhoI;</t> lane 17, XbaI.
    Ecori Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori xhoi/product/TaKaRa
    Average 85 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    ecori xhoi - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    Image Search Results


    Effect of CD22ΔE12 vs. CD22 WT on clonogenicity and self-renewal rate of ALL cells. [A1] Depicted is a representative 1% agarose gel showing the PCR products of FL CD22 and CD22ΔE12–14. [A.2] Subcloning of the CD22 FL or CD22ΔE12–14 genes into the lentiviral pCL6-2AEGwo vector through NheI and XhoI restriction sites was confirmed through restriction enzyme digestion and DNA sequencing. Depicted is a representative 1% agarose gel showing that the generated lentiviral constructs have the correct size FL CD22 (2.5-kb) or CD22ΔE12–14 (2.2-kb) inserts. [B.1] In order to confirm that the lentiviral vectors can be used to achieve expression of FL and truncated CD22 in human cells, 293T cells were transfected with lentiviral constructs for FL CD22, CD22ΔE12–14, as well as pCL6-2AEGwo lentiviral vector without any subcloned CD22. 48 h post transfection cells were examined for FL CD22 and CD22ΔE12–14 mRNA by RT-PCR using the P7, P9, and P10 primer pairs. The P7 primer set was used to amplify a 182-bp region (c.2180–c.2361) of the CD22 cDNA extending from Exon 11 to Exon 13 and spanning the entire Exon 12. The P9 primer set was used amplify a 160-bp region (c.2304–c.2463) of the CD22 cDNA extending from Exon 12 to Exon 14 and spanning the entire Exon 13. The P10 primer set was used to amplify a 213-bp region (c.433–c.645) of Exon 4 of the CD22 cDNA present in both wildtype CD22 and CD22ΔE12–14 mRNA species. As expected all primer sets yielded PCR products in cells transfected with the FL CD22 vector and only the P10 primer set yielded a PCR product in cells transfected with the CD22ΔE12–14 vector. [B.2 and B3] The increased expression levels of the full-length and truncated proteins in transduced 293-T cells (depicted in B.2) and ALL-1 cells (depicted in B.3) were confirmed to be similar by Western blot analysis done at 96 h post-transduction. [C D] ALL cell lines DAUDI (Burkitt's leukemia/B-ALL) (panels C1 C.2) and ALL1 (BCR-ABL + B-precursor ALL) (panel C3) were transduced (trans) with wildtype and mutant human CD22 genes using the pCL6-2AEGwo lentiviral vector and then assayed for colony formation in semi-solid methylcellulose cultures without additional stroma support or cytokines. Colony formation was examined using an inverted Nikon Eclipse TS100 microscope with an Epifluorescence attachment. Images were taken using a Digital Sight DS-2MBW Nikon camera (System magnification: 100× or 200 × as indicated). Green fluorescence of colonies resulting from GFP expression confirms successful transduction of the cell lines. [D] Depicted are bar graphs comparing the mean colony numbers in cultures of untransduced and transduced cells.

    Journal: EBioMedicine

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies

    doi: 10.1016/j.ebiom.2014.10.013

    Figure Lengend Snippet: Effect of CD22ΔE12 vs. CD22 WT on clonogenicity and self-renewal rate of ALL cells. [A1] Depicted is a representative 1% agarose gel showing the PCR products of FL CD22 and CD22ΔE12–14. [A.2] Subcloning of the CD22 FL or CD22ΔE12–14 genes into the lentiviral pCL6-2AEGwo vector through NheI and XhoI restriction sites was confirmed through restriction enzyme digestion and DNA sequencing. Depicted is a representative 1% agarose gel showing that the generated lentiviral constructs have the correct size FL CD22 (2.5-kb) or CD22ΔE12–14 (2.2-kb) inserts. [B.1] In order to confirm that the lentiviral vectors can be used to achieve expression of FL and truncated CD22 in human cells, 293T cells were transfected with lentiviral constructs for FL CD22, CD22ΔE12–14, as well as pCL6-2AEGwo lentiviral vector without any subcloned CD22. 48 h post transfection cells were examined for FL CD22 and CD22ΔE12–14 mRNA by RT-PCR using the P7, P9, and P10 primer pairs. The P7 primer set was used to amplify a 182-bp region (c.2180–c.2361) of the CD22 cDNA extending from Exon 11 to Exon 13 and spanning the entire Exon 12. The P9 primer set was used amplify a 160-bp region (c.2304–c.2463) of the CD22 cDNA extending from Exon 12 to Exon 14 and spanning the entire Exon 13. The P10 primer set was used to amplify a 213-bp region (c.433–c.645) of Exon 4 of the CD22 cDNA present in both wildtype CD22 and CD22ΔE12–14 mRNA species. As expected all primer sets yielded PCR products in cells transfected with the FL CD22 vector and only the P10 primer set yielded a PCR product in cells transfected with the CD22ΔE12–14 vector. [B.2 and B3] The increased expression levels of the full-length and truncated proteins in transduced 293-T cells (depicted in B.2) and ALL-1 cells (depicted in B.3) were confirmed to be similar by Western blot analysis done at 96 h post-transduction. [C D] ALL cell lines DAUDI (Burkitt's leukemia/B-ALL) (panels C1 C.2) and ALL1 (BCR-ABL + B-precursor ALL) (panel C3) were transduced (trans) with wildtype and mutant human CD22 genes using the pCL6-2AEGwo lentiviral vector and then assayed for colony formation in semi-solid methylcellulose cultures without additional stroma support or cytokines. Colony formation was examined using an inverted Nikon Eclipse TS100 microscope with an Epifluorescence attachment. Images were taken using a Digital Sight DS-2MBW Nikon camera (System magnification: 100× or 200 × as indicated). Green fluorescence of colonies resulting from GFP expression confirms successful transduction of the cell lines. [D] Depicted are bar graphs comparing the mean colony numbers in cultures of untransduced and transduced cells.

    Article Snippet: The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Subcloning, Plasmid Preparation, DNA Sequencing, Generated, Construct, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transduction, Mutagenesis, Microscopy, Fluorescence

    Schematic representation of the expression vector, pET32a-mBD2. The cDNA for mature mBD2 were amplified using PCR from the pcDNA3.1(+)-mBD2 plasmid. PCR product was cleaved by Xho I and Kpn I, and the mBD2 fragment was inserted into similarly digested pET32a(+) vector to construct the expression vector, pET32a-mBD2.

    Journal: Brazilian Journal of Microbiology

    Article Title: Expression of mouse beta defensin 2 in escherichia coli and its broad-spectrum antimicrobial activity

    doi: 10.1590/S1517-838220110003000043

    Figure Lengend Snippet: Schematic representation of the expression vector, pET32a-mBD2. The cDNA for mature mBD2 were amplified using PCR from the pcDNA3.1(+)-mBD2 plasmid. PCR product was cleaved by Xho I and Kpn I, and the mBD2 fragment was inserted into similarly digested pET32a(+) vector to construct the expression vector, pET32a-mBD2.

    Article Snippet: The PCR conditions were following: 94°C, 4 min; 30 cycles of 94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 72°C, 5 min. PCR product was cleaved by Xho I and Kpn I , and the mBD2 fragment was inserted into similarly digested pET32a(+) (Novagen, Shanghai, China) to construct the expression vector pET32a-mBD2.

    Techniques: Expressing, Plasmid Preparation, Amplification, Polymerase Chain Reaction, Construct

    (A) Digestion of pTY-α-amylase with Nhe I and Xho I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei

    doi: 10.1016/j.phrp.2015.10.003

    Figure Lengend Snippet: (A) Digestion of pTY-α-amylase with Nhe I and Xho I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Article Snippet: P. woesei chromosomal DNA was used as a source of the α-amylase gene and polymerase chain reaction (PCR) amplification was done with the following primers: 5′- GCTAGC TTGGAGCTTGAAGAGGGAG-3′ and 5′- GAGACC ACAATAACTCCATACGGAG-3′ containing recognition sites for restriction endonucleases Nhe I and Xho I (Fermentas; Vilnius, Lithuania).

    Techniques: Polymerase Chain Reaction, SDS Page, Expressing, Western Blot, Marker, Polyacrylamide Gel Electrophoresis

    Graphical representation of pET28a-bglA. Expression vector was created by insertion of bglA gene between NcoI and XhoI sites. Inserted sequence was adjacent to lac operator and terminates at 6xHis-tag included in the frame. Kan R : kanamycin resistance gene, Ori: origin of replication. The sketch was created using SnapGene® software (GSL Biotech).

    Journal: BioMed Research International

    Article Title: Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli

    doi: 10.1155/2019/9308593

    Figure Lengend Snippet: Graphical representation of pET28a-bglA. Expression vector was created by insertion of bglA gene between NcoI and XhoI sites. Inserted sequence was adjacent to lac operator and terminates at 6xHis-tag included in the frame. Kan R : kanamycin resistance gene, Ori: origin of replication. The sketch was created using SnapGene® software (GSL Biotech).

    Article Snippet: NcoI and XhoI (Fermentas Inc., Ontario, Canada) restriction enzymes were used for restriction of cloning and expression vectors.

    Techniques: Expressing, Plasmid Preparation, Sequencing, Software

    pJ915-rhChymase vector map The P. pastoris ] using 5’ XhoI and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.

    Journal: Protein expression and purification

    Article Title: Expression of Recombinant Human Mast Cell Chymase with Asn-linked Glycans in Glycoengineered Pichia pastoris

    doi: 10.1016/j.pep.2014.08.005

    Figure Lengend Snippet: pJ915-rhChymase vector map The P. pastoris ] using 5’ XhoI and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.

    Article Snippet: The DNA encoding human Chymase (hChymase) with an added N-terminal Kex2 protease cleavage site was isolated from the previously described plasmid pPICzα-rhChymase [ ] using simultaneous digestion with XhoI and NotI restriction endonucleases (Thermo Scientific, FastDigest).

    Techniques: Plasmid Preparation, Expressing, Marker, Electroporation

    Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, SpeI; lane 16, XhoI; lane 17, XbaI.

    Journal: Applied and Environmental Microbiology

    Article Title: Isolation and Characterization of a Cyanophage Infecting the Toxic Cyanobacterium Microcystis aeruginosa

    doi: 10.1128/AEM.72.2.1239-1247.2006

    Figure Lengend Snippet: Ma-LMM01 genomic DNA digested with various restriction enzymes. Lanes 1 and 18, 1-kb ladder marker; lane 2, BamHI; lane 3, EcoRI; lane 4, EcoRV; lane 5, HincII; lane 6, HindIII; lane 7, NotI; lane 8, PstI; lane 9, SacI; lane 10, SalI; lane 11, ScaI; lane 12, SmaI; lanes 13 and 14, lambda/HindIII marker; lane 15, SpeI; lane 16, XhoI; lane 17, XbaI.

    Article Snippet: Using the manufacturers' recommendations, we tested the sensitivity of the phage nucleic acid to RNase A (0.01 ng μl−1 ; 37°C for 1 h; Nippon Gene Co., Ltd.), DNase I (0.02 ng μl−1 ; 37°C for 1 h; Promega Co., Ltd.), and the following 14 restriction enzymes, which were incubated for 16 h: SpeI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), XhoI (0.45 U μl−1 ; 37°C; TOYOBO Co., Ltd.), XbaI (0.5 U μl−1 ; 37°C; Roche Molecular Biochemicals), BamHI (0.6 U μl−1 ; 37°C; TOYOBO Co., Ltd.), EcoRI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), EcoRV (0.6 U μl−1 ; 37°C; TOYOBO Co., Ltd.), HincII (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), HindIII (0.5 U μl−1 ; 37°C; Nippon Gene Co., Ltd.), NotI (0.5 U μl−1 ; 37°C; New England Biolabs Inc.), PstI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), SacI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), SalI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), ScaI (0.5 U μl−1 ; 37°C; TOYOBO Co., Ltd.), and SmaI (0.6 U μl−1 ; 30°C; TOYOBO Co., Ltd.).

    Techniques: Marker