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  • 99
    New England Biolabs xhoi
    Xhoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher xhoi
    Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    xhoi  (TaKaRa)
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    TaKaRa xhoi
    Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher xho i
    Xho I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5850 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore xho
    Xho, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs xho i
    Construction and expression of the secretion plasmid <t>pPG612.1-BLF</t> in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and <t>XhoI,</t> and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture
    Xho I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4879 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bamhi xhoi sites
    Construction and expression of the secretion plasmid <t>pPG612.1-BLF</t> in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and <t>XhoI,</t> and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture
    Bamhi Xhoi Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ecori xhoi sites
    Construction and expression of the secretion plasmid <t>pPG612.1-BLF</t> in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and <t>XhoI,</t> and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture
    Ecori Xhoi Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher fastdigest xhoi
    Construction and expression of the secretion plasmid <t>pPG612.1-BLF</t> in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and <t>XhoI,</t> and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture
    Fastdigest Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    Construction and expression of the secretion plasmid pPG612.1-BLF in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and XhoI, and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture

    Journal: BMC Microbiology

    Article Title: Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis

    doi: 10.1186/s12866-018-1370-x

    Figure Lengend Snippet: Construction and expression of the secretion plasmid pPG612.1-BLF in L.casei . a The synthetic BLF gene fragment (2.1kp) was digested with restriction enzymes BamHI and XhoI, and ligated into the sticky end of the plasmid pPG612.1 which was also digested with the same restriction enzyme, resulting in the plasmid pPG612.1-BLF (5.6kp). b The plasmid pPG612.1-BLF was electroporated into L.casei using a BioRad GenePulser with single electric pulse (voltage, 1.5 kV; capacitance, 25 μF; and resistance, 400 Ω.). PCR amplification of the BamHI site and XhoI site of the plasmid pPG612.1-BLF which was extracted from the L.casei /pPG612.1-BLF strain resulted in 500 bp and 800 bp products, respectively. Lane 1, PCR product of XhoI site; Lane 2, PCR product of BamHI site. M, DNA maker. c BLF was detected in the supernatant and pellet of L.casei /pPG612.1-BLF culture by Western blotting, indicating the expression and secretion of BLF by L.casei /pPG612.1-BLF. Lane 1, supernatant of L.casei /pPG612.1-BLF culture; Lane 2, pellet of L.casei /pPG612.1-BLF culture; Lane 3, supernatant of L.casei /pPG612.1 culture; Lane 4, pellet of L.casei /pPG612.1 culture

    Article Snippet: The BLF fragment was retrieved from the pUC57-BLF using BamHI and XhoI enzymes (New England Biolabs Inc., Ipswich, MA, USA) and ligated into the corresponding sites of pPG612.1, generating the plasmid pPG612.1-BLF.

    Techniques: Expressing, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Western Blot