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    New England Biolabs xba1
    A chromatin loop containing the 5′ and 3′ Wnt/β-catenin responsive enhancers is present at MYC in HCT116 cells. ( A ) Diagram of the human MYC ). An arrow above E1 marks the major MYC transcription start site. BstY1 restriction endonuclease sites are depicted by triangles and stunted arrows mark the positions of oligonucleotides used in the 3C assays (labeled B1-B8). ( B ) Analysis by 3C of the MYC locus in HCT116 cells. A PCR product was generated with B4 and B8 only (labeled MYC 5′3′). LC is a loading control and S is a DNA standard. ( C ) A diagram of the MYC locus as in A except that triangles mark <t>Xba1</t> restriction endonuclease sites and stunted arrows mark the position of ×1, ×2, and ×3 oligonucleotides. ( D ) Same as B except that Xba1 was used in 3C reactions and purified DNA was amplified with ×1 and ×3.
    Xba1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xba1/product/New England Biolabs
    Average 95 stars, based on 211 article reviews
    Price from $9.99 to $1999.99
    xba1 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    80
    New England Biolabs xba1 restriction enzyme
    A chromatin loop containing the 5′ and 3′ Wnt/β-catenin responsive enhancers is present at MYC in HCT116 cells. ( A ) Diagram of the human MYC ). An arrow above E1 marks the major MYC transcription start site. BstY1 restriction endonuclease sites are depicted by triangles and stunted arrows mark the positions of oligonucleotides used in the 3C assays (labeled B1-B8). ( B ) Analysis by 3C of the MYC locus in HCT116 cells. A PCR product was generated with B4 and B8 only (labeled MYC 5′3′). LC is a loading control and S is a DNA standard. ( C ) A diagram of the MYC locus as in A except that triangles mark <t>Xba1</t> restriction endonuclease sites and stunted arrows mark the position of ×1, ×2, and ×3 oligonucleotides. ( D ) Same as B except that Xba1 was used in 3C reactions and purified DNA was amplified with ×1 and ×3.
    Xba1 Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xba1 restriction enzyme/product/New England Biolabs
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xba1 restriction enzyme - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    Image Search Results


    A chromatin loop containing the 5′ and 3′ Wnt/β-catenin responsive enhancers is present at MYC in HCT116 cells. ( A ) Diagram of the human MYC ). An arrow above E1 marks the major MYC transcription start site. BstY1 restriction endonuclease sites are depicted by triangles and stunted arrows mark the positions of oligonucleotides used in the 3C assays (labeled B1-B8). ( B ) Analysis by 3C of the MYC locus in HCT116 cells. A PCR product was generated with B4 and B8 only (labeled MYC 5′3′). LC is a loading control and S is a DNA standard. ( C ) A diagram of the MYC locus as in A except that triangles mark Xba1 restriction endonuclease sites and stunted arrows mark the position of ×1, ×2, and ×3 oligonucleotides. ( D ) Same as B except that Xba1 was used in 3C reactions and purified DNA was amplified with ×1 and ×3.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A ?-catenin/TCF-coordinated chromatin loop at MYC integrates 5? and 3? Wnt responsive enhancers

    doi: 10.1073/pnas.0912294107

    Figure Lengend Snippet: A chromatin loop containing the 5′ and 3′ Wnt/β-catenin responsive enhancers is present at MYC in HCT116 cells. ( A ) Diagram of the human MYC ). An arrow above E1 marks the major MYC transcription start site. BstY1 restriction endonuclease sites are depicted by triangles and stunted arrows mark the positions of oligonucleotides used in the 3C assays (labeled B1-B8). ( B ) Analysis by 3C of the MYC locus in HCT116 cells. A PCR product was generated with B4 and B8 only (labeled MYC 5′3′). LC is a loading control and S is a DNA standard. ( C ) A diagram of the MYC locus as in A except that triangles mark Xba1 restriction endonuclease sites and stunted arrows mark the position of ×1, ×2, and ×3 oligonucleotides. ( D ) Same as B except that Xba1 was used in 3C reactions and purified DNA was amplified with ×1 and ×3.

    Article Snippet: Crosslinked chromatin was digested with either 20 μL (200 U) of BstY1 or 10 μL (200 U) Xba1 (New England Biolabs) overnight at 37°C.

    Techniques: Labeling, Polymerase Chain Reaction, Generated, Purification, Amplification