x-gal staining Search Results


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  • 94
    Thermo Fisher x gal staining
    X Gal Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 31 article reviews
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    x gal staining - by Bioz Stars, 2020-08
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    99
    Millipore x gal staining solution
    X Gal Staining Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 194 article reviews
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    99
    Millipore 5 bromo 4 chloro 3 indolyl beta d galactopyranoside x gal staining solution
    5 Bromo 4 Chloro 3 Indolyl Beta D Galactopyranoside X Gal Staining Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3 article reviews
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    88
    Genlantis x gal staining
    X Gal Staining, supplied by Genlantis, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 19 article reviews
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    88
    Genlantis x gal staining kit
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Kit, supplied by Genlantis, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining kit/product/Genlantis
    Average 88 stars, based on 56 article reviews
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    x gal staining kit - by Bioz Stars, 2020-08
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    93
    Mirus Bio x gal staining kit
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Kit, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 3 article reviews
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    90
    Cell Signaling Technology Inc x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining solution/product/Cell Signaling Technology Inc
    Average 90 stars, based on 31 article reviews
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    91
    Beyotime x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 7 article reviews
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    98
    Millipore x gal staining kit
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining kit/product/Millipore
    Average 98 stars, based on 23 article reviews
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    90
    Genlantis x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Genlantis, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining solution/product/Genlantis
    Average 90 stars, based on 7 article reviews
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    x gal staining solution - by Bioz Stars, 2020-08
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    90
    Promega x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 40 article reviews
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    92
    Meridian Life Science x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 4 article reviews
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    92
    Mirus Bio x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining solution/product/Mirus Bio
    Average 92 stars, based on 3 article reviews
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    99
    Millipore x gal staining solutions
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solutions, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining solutions/product/Millipore
    Average 99 stars, based on 10 article reviews
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    89
    Millipore x gal staining
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining/product/Millipore
    Average 89 stars, based on 63 article reviews
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    85
    Boehringer Mannheim prepared x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    Prepared X Gal Staining Solution, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prepared x gal staining solution/product/Boehringer Mannheim
    Average 85 stars, based on 3 article reviews
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    prepared x gal staining solution - by Bioz Stars, 2020-08
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    99
    Millipore x gal staining buffer
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining buffer/product/Millipore
    Average 99 stars, based on 24 article reviews
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    99
    BioVision x gal staining solution
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    X Gal Staining Solution, supplied by BioVision, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x gal staining solution/product/BioVision
    Average 99 stars, based on 4 article reviews
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    96
    Thermo Fisher beta gal x gal staining
    Kinetics <t>assay</t> of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by <t>X-gal</t> <t>staining</t> for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.
    Beta Gal X Gal Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 2 article reviews
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    Image Search Results


    Kinetics assay of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by X-gal staining for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.

    Journal: International Journal of Biological Sciences

    Article Title: Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production

    doi: 10.7150/ijbs.27411

    Figure Lengend Snippet: Kinetics assay of the JFH1-AM120-LacZ HCV reporter after multiple passages. The cells transfected with the RNA of JFH1-AM120-LacZ were passaged at every three days for a total of 15 days. Supernatants collected were designated P1 to P5. The double titrations were carried out by X-gal staining for β-galactosidase and immunofluoresence staining for NS5A protein (see Materials and Methods). Experiments were performed three times and the data presented as the mean ± SD.

    Article Snippet: Using the same method, additional cells were transfected with JFH-AM120-LacZ RNA or control RNAs and fixed with paraformaldehyde, stained using an X-gal staining assay Kit (Genlantis) and visualized the cell with blue color under bright field microscope.

    Techniques: Transfection, Staining

    Analysis of NS5A-LacZ activity followed RNA transfection of JFH1-AM120-LacZ . (A) Huh7.5 cells were transfected with the RNA of JFH1-AM120-LacZ and controls of JFH1-AM120, JFH1-AM120-EGFP and JFH1-AM120-Rluc as described in Methods. Three days post-transfection , the absorption peaks of β-Galactosidase activity were measured using a Bio-Tec plate reader at 405 nm. The values were relative to JFH1-AM120-LacZ. Experiments were performed three times and the data presented as the mean ± SD. (B) Three days post-transfection of Huh7.5 cells in 24-well plates with RNA of JFH1-AM120-LacZ, cells were fixed with 4% paraformaldehyde followed X-gal staining. Cover slips were visualized and images were taken (100x) by bright field microscopy. Experiments were performed two times and representative results are shown. (C) Co-localization of NS5A and β-galactosidase. Three days post-transfection of Huh7.5 cells in 24-well plates with RNA of JFH1-AM120-LacZ, Cells were fixed with 4% paraformaldehyde and were immunostained with anti-NS5A antibody (red) and Nuclei were counterstained using DAPI (blue). Images were taken by immunofluorescence microscopy (200x). Then the coverslip was washed and were processed with X-gal staining followed visualizing and imaging by bright field microscopy (200x). Colocalization assay was performed by Photoshop CC software. Experiments were performed three times and representative results are shown.

    Journal: International Journal of Biological Sciences

    Article Title: Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production

    doi: 10.7150/ijbs.27411

    Figure Lengend Snippet: Analysis of NS5A-LacZ activity followed RNA transfection of JFH1-AM120-LacZ . (A) Huh7.5 cells were transfected with the RNA of JFH1-AM120-LacZ and controls of JFH1-AM120, JFH1-AM120-EGFP and JFH1-AM120-Rluc as described in Methods. Three days post-transfection , the absorption peaks of β-Galactosidase activity were measured using a Bio-Tec plate reader at 405 nm. The values were relative to JFH1-AM120-LacZ. Experiments were performed three times and the data presented as the mean ± SD. (B) Three days post-transfection of Huh7.5 cells in 24-well plates with RNA of JFH1-AM120-LacZ, cells were fixed with 4% paraformaldehyde followed X-gal staining. Cover slips were visualized and images were taken (100x) by bright field microscopy. Experiments were performed two times and representative results are shown. (C) Co-localization of NS5A and β-galactosidase. Three days post-transfection of Huh7.5 cells in 24-well plates with RNA of JFH1-AM120-LacZ, Cells were fixed with 4% paraformaldehyde and were immunostained with anti-NS5A antibody (red) and Nuclei were counterstained using DAPI (blue). Images were taken by immunofluorescence microscopy (200x). Then the coverslip was washed and were processed with X-gal staining followed visualizing and imaging by bright field microscopy (200x). Colocalization assay was performed by Photoshop CC software. Experiments were performed three times and representative results are shown.

    Article Snippet: Using the same method, additional cells were transfected with JFH-AM120-LacZ RNA or control RNAs and fixed with paraformaldehyde, stained using an X-gal staining assay Kit (Genlantis) and visualized the cell with blue color under bright field microscope.

    Techniques: Activity Assay, Transfection, Staining, Microscopy, Immunofluorescence, Imaging, Software

    Infectivity assay of virus particles followed RNAs transfection of JFH1-AM120-LacZ and controls . The RNA of JFH1-AM120-LacZ and controls of JFH1-AM120, JFH1-AM120-EGFP and JFH1-AM120-Rluc were electroporated into Huh-7.5 and the infectivity titers in the cultured supernatants at the 6th day were measured (Described in Material and Method). The viral titer is expressed as focus-forming units per ml of supernatant (ffu/ml) as determined by the average number of NS5A-positive foci detected by immunofluorescence for NS5A (Mock, JFH-AM120 and JFH-AM120-Rluc), or directly visualized EGFP positive cells(JFH1-AM120-EGFP) and detected blue color cells after X-gal staining (JFH1-AM120-LacZ). Assays were performed three times and the data are presented as mean ± standard deviation. (** P

    Journal: International Journal of Biological Sciences

    Article Title: Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production

    doi: 10.7150/ijbs.27411

    Figure Lengend Snippet: Infectivity assay of virus particles followed RNAs transfection of JFH1-AM120-LacZ and controls . The RNA of JFH1-AM120-LacZ and controls of JFH1-AM120, JFH1-AM120-EGFP and JFH1-AM120-Rluc were electroporated into Huh-7.5 and the infectivity titers in the cultured supernatants at the 6th day were measured (Described in Material and Method). The viral titer is expressed as focus-forming units per ml of supernatant (ffu/ml) as determined by the average number of NS5A-positive foci detected by immunofluorescence for NS5A (Mock, JFH-AM120 and JFH-AM120-Rluc), or directly visualized EGFP positive cells(JFH1-AM120-EGFP) and detected blue color cells after X-gal staining (JFH1-AM120-LacZ). Assays were performed three times and the data are presented as mean ± standard deviation. (** P

    Article Snippet: Using the same method, additional cells were transfected with JFH-AM120-LacZ RNA or control RNAs and fixed with paraformaldehyde, stained using an X-gal staining assay Kit (Genlantis) and visualized the cell with blue color under bright field microscope.

    Techniques: Infection, Transfection, Cell Culture, Immunofluorescence, Staining, Standard Deviation