wolbachia titer Search Results


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    Zymo Research zr tissue insect dna kit microprep
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. <t>obscurior</t> Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p
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    Image Search Results


    Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. obscurior Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p

    Journal: bioRxiv

    Article Title: Cytoplasmic incompatibility between Old and New World populations of a tramp ant

    doi: 10.1101/2020.03.13.988675

    Figure Lengend Snippet: Mean weekly egg numbers produced by intra- and inter-population crosses of the ant C. obscurior Mean weekly egg numbers produced by Brazilian (BR) and Japanese (JP) queens mated to males from their own or from a different population over a period of six weeks. JP ants were collected from colonies that either carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP) or did not (JP we- ). Males used in “CI rescue” crosses were collected from JP colonies with and without Cand . Westeberhardia cardiocondylae, which had been treated with the antibiotic rifampicin (JP rif+ ; JP we- rif+ ). Numbers below box plots indicate the number of replicates. Differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p

    Article Snippet: Consistently high titers in workers indicate that Wolbachia do not have deleterious effects on worker performance in C. obscurior , unlike Wolbachia in Acromyrmex ants .

    Techniques: Produced, MANN-WHITNEY

    Wolbachia spp. phylogeny and genome synteny (A) Bayesian phylogenetic placement of Wolbachia strains sequenced from C. obscurior ( w Cobs-BR and w Cobs-JP). Anaplasma phagocytophilum and Ehrlichia canis were used as outgroups. For Wolbachia , names at tips correspond to the host from which they were sequenced/isolated. Red dots are used to highlight the newly sequenced strains. Next to the binomial names, strain names are shown in grey and silhouettes for the host are sketched in black. Horizontal bars delimit Wolbachia supergroups (A-F). *=1.0 posterior probability. (B) Genome-wise nucleotide-based synteny plot between w Cobs-BR and w Cobs-JP. Red is used to indicate a direct match and blue a reverse complement match.

    Journal: bioRxiv

    Article Title: Cytoplasmic incompatibility between Old and New World populations of a tramp ant

    doi: 10.1101/2020.03.13.988675

    Figure Lengend Snippet: Wolbachia spp. phylogeny and genome synteny (A) Bayesian phylogenetic placement of Wolbachia strains sequenced from C. obscurior ( w Cobs-BR and w Cobs-JP). Anaplasma phagocytophilum and Ehrlichia canis were used as outgroups. For Wolbachia , names at tips correspond to the host from which they were sequenced/isolated. Red dots are used to highlight the newly sequenced strains. Next to the binomial names, strain names are shown in grey and silhouettes for the host are sketched in black. Horizontal bars delimit Wolbachia supergroups (A-F). *=1.0 posterior probability. (B) Genome-wise nucleotide-based synteny plot between w Cobs-BR and w Cobs-JP. Red is used to indicate a direct match and blue a reverse complement match.

    Article Snippet: Consistently high titers in workers indicate that Wolbachia do not have deleterious effects on worker performance in C. obscurior , unlike Wolbachia in Acromyrmex ants .

    Techniques: Isolation

    Localization of endosymbiotic bacteria in male adult abdomens of C. obscurior via fluorescence in situ hybridization. Wolbachia were specifically stained with two Cy5-labeled probes (magenta), and Cand . Westeberhardia with a Cy3-labeled probe (green). Host cell nuclei are counterstained with DAPI (blue). (A) Transversal section of C. obscurior JP male abdomen showing the testes (t), the Cand . Westeberhardia-containing bacteriomes (B) and the gut (g). The areas indicated by rectangles are displayed in higher magnification showing Wolbachia -infected seminiferous tubules (B) and bacteriomes co-infected with Cand . Westeberhardia and Wolbachia (C). (D) C. obscurior BR male testes and gut epithelium infected with Wolbachia . Scale bars: 50 µm (A) and 20 µm (B-D).

    Journal: bioRxiv

    Article Title: Cytoplasmic incompatibility between Old and New World populations of a tramp ant

    doi: 10.1101/2020.03.13.988675

    Figure Lengend Snippet: Localization of endosymbiotic bacteria in male adult abdomens of C. obscurior via fluorescence in situ hybridization. Wolbachia were specifically stained with two Cy5-labeled probes (magenta), and Cand . Westeberhardia with a Cy3-labeled probe (green). Host cell nuclei are counterstained with DAPI (blue). (A) Transversal section of C. obscurior JP male abdomen showing the testes (t), the Cand . Westeberhardia-containing bacteriomes (B) and the gut (g). The areas indicated by rectangles are displayed in higher magnification showing Wolbachia -infected seminiferous tubules (B) and bacteriomes co-infected with Cand . Westeberhardia and Wolbachia (C). (D) C. obscurior BR male testes and gut epithelium infected with Wolbachia . Scale bars: 50 µm (A) and 20 µm (B-D).

    Article Snippet: Consistently high titers in workers indicate that Wolbachia do not have deleterious effects on worker performance in C. obscurior , unlike Wolbachia in Acromyrmex ants .

    Techniques: Fluorescence, In Situ Hybridization, Staining, Labeling, Infection

    Total pupae numbers and pupae sex ratios produced by intra- and inter-population crosses of the ant C. obscurior Total number of pupae (A) and sex ratio of pupae (B) produced in crosses between Brazilian (BR) and Japanese (JP) queens and males over a period of 12 weeks. All JP ants were collected from colonies that carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP). Males used in BR x JP rif+ crosses were collected from JP colonies that had been treated with the antibiotic rifampicin. Numbers below box plots indicate the number of replicates. For total pupae numbers, differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Sex ratios produced by different crosses were compared with a generalized linear model with logit link followed by manual Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p

    Journal: bioRxiv

    Article Title: Cytoplasmic incompatibility between Old and New World populations of a tramp ant

    doi: 10.1101/2020.03.13.988675

    Figure Lengend Snippet: Total pupae numbers and pupae sex ratios produced by intra- and inter-population crosses of the ant C. obscurior Total number of pupae (A) and sex ratio of pupae (B) produced in crosses between Brazilian (BR) and Japanese (JP) queens and males over a period of 12 weeks. All JP ants were collected from colonies that carried the main endosymbiont Candidatus Westeberhardia cardiocondylae (JP). Males used in BR x JP rif+ crosses were collected from JP colonies that had been treated with the antibiotic rifampicin. Numbers below box plots indicate the number of replicates. For total pupae numbers, differences between groups were tested with pairwise Mann-Whitney-U-tests followed by Bonferroni-Holms correction of p-values. Sex ratios produced by different crosses were compared with a generalized linear model with logit link followed by manual Bonferroni-Holms correction of p-values. Letters above boxplots indicate statistically significant differences at p

    Article Snippet: Consistently high titers in workers indicate that Wolbachia do not have deleterious effects on worker performance in C. obscurior , unlike Wolbachia in Acromyrmex ants .

    Techniques: Produced, MANN-WHITNEY