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    Promega wizard plus sv minipreps dna purification promega
    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV <t>Minipreps</t> (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.
    Wizard Plus Sv Minipreps Dna Purification Promega, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 699 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Journal: Journal of Bacteriology

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling

    doi: 10.1128/JB.184.10.2755-2766.2002

    Figure Lengend Snippet: Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Article Snippet: Plasmid DNA was isolated by using either the Wizard Plus SV Minipreps purification system (Promega) according to the manufacturers protocol or the XSP buffer extraction method described by De Ungria et al. ( ).

    Techniques: Purification, Isolation, Agarose Gel Electrophoresis, Marker