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  • 99
    Thermo Fisher wizard genomic dna purification kit
    Wizard Genomic Dna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega wizard genomic dna gdna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizard Genomic Dna Gdna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 669 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna gdna purification kit/product/Promega
    Average 88 stars, based on 669 article reviews
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    99
    Promega wizardr genomic dna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizardr Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies wizard genomic dna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizard Genomic Dna Purification Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega wizard genomic dna purification kit protocol
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizard Genomic Dna Purification Kit Protocol, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna purification kit protocol/product/Promega
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    86
    Promega wizard sv96 genomic dna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizard Sv96 Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega wizare genomic dna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizare Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizare genomic dna purification kit/product/Promega
    Average 88 stars, based on 46 article reviews
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    wizare genomic dna purification kit - by Bioz Stars, 2020-08
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    90
    Promega wizardâ genomic dna purification kit
    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . <t>gonorrhoeae</t> FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing <t>DNA</t> sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.
    Wizardâ Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizardâ genomic dna purification kit/product/Promega
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    Image Search Results


    Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . gonorrhoeae FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing DNA sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.

    Journal: PLoS Pathogens

    Article Title: SliC is a surface-displayed lipoprotein that is required for the anti-lysozyme strategy during Neisseria gonorrhoeae infectionNeisseria gonorrhoeae employs two protein inhibitors to evade killing by human lysozyme

    doi: 10.1371/journal.ppat.1007081

    Figure Lengend Snippet: Sequence alignment, phylogenetic analysis and conservation of SliC. (A) The amino acid sequence of SliC from N . gonorrhoeae FA1090 (WP 003688168) was aligned with MliC for E . coli (AJE56069), S . enterica (NP 460410), and P . aeruginosa (AAG04256) sequences downloaded from NCBI with the Clustal Omega online tool (Clustal 2.1; https://www.ebi.ac.uk/Tools/msa/clustalo/ ) using the default alignment parameters. The predicted lipoprotein signal peptide is shown in aquamarine. The conserved regions of the COG3895 domain in the MliC/PliC family, SxSGAxY and YxxxTKG are shown in red and green boxes, respectively. Preserved residues are marked in color within conserved regions and by asterisks (*) throughout the sequences. The residues S83 and K103, predicted to be involved in interaction with c-type lysozyme are designated by red and green arrows, respectively. (B) A phylogenetic comparison of SliC, MliC/PliC and ACP. The tree was constructed using the Jones-Taylor-Thornton model [ 58 ] in MEGA. Neighbor-Join and BioNJ algorithms were applied to a pairwise-distance matrix derived from the JTT model to obtain an initial tree for the heuristic search. The phylogenies were tested by 500 bootstrap iterations, and the tree with the highest log likelihood is presented. (C) A phylogenetic comparison of SliC alleles in 3787 isolates of N . gonorrhoeae deposited to the PubMLST was performed as described above. The dominant allele (29) present in over 97% gonococcal isolates and in FA1090 is denoted in a red box. (D) Analysis of single nucleotide polymorphisms of SliC (locus NEIS1425) in N . gonorrhoeae was performed by comparing DNA sequences between gonococcal isolates deposited to PubMLST ( https://pubmlst.org/neisseria/ , as of April 11, 2018). (E) A panel constituting 37 N . gonorrhoeae isolates that are distinct genetically, geographically, and temporally, including common laboratory strains; clinical isolates collected from public health clinics in Baltimore from 1991 to 1994 and Seattle from 2011 to 2016; and the 14 isolates constituting the 2016 WHO reference strains were grown concurrently on solid medium for 20 h in 5% CO 2 at 37° C. Cells were collected, lysed and processed for immunoblotting. (F) Whole-cell lysates of different Neisseria , including pathogenic N . meningitidis MC58, commensal N . lactamica NLI83/-01, and a human opportunistic pathogen N . weaveri 1032 were separated by SDS-PAGE and probed with antisera. In all experiments, samples were matched by equivalent OD 600 units, resolved in a 4 to 20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblots were performed using polyclonal rabbit antisera against SliC. Cell lysates of wild type FA1090 and isogenic Δ sliC were loaded as controls for the experiments.

    Article Snippet: N . gonorrhoeae FA1090 (NC_002946) genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).

    Techniques: Sequencing, Construct, Derivative Assay, SDS Page, Western Blot

    The web of DNA fibres and its association with Psl polysaccharide and bacterial cell membrane in the air–liquid interface biofilms (pellicles) of P. aeruginosa .

    Journal: Environmental microbiology reports

    Article Title: The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa

    doi: 10.1111/1758-2229.12252

    Figure Lengend Snippet: The web of DNA fibres and its association with Psl polysaccharide and bacterial cell membrane in the air–liquid interface biofilms (pellicles) of P. aeruginosa .

    Article Snippet: Genomic DNAs of neutrophils, P. aeruginosa and S. aureus ATCC 6538 were purified with Wizard Genomic DNA Purification Kits (Promega).

    Techniques:

    The eDNA and dead bacteria in pellicles of P. aeruginosa PAO1 were stained by Propidium iodide (PI) to confirm that fibre-like DNA was eDNA. Shown were the optical sectioned images in the middle of pellicles. (A) PI (red) and SYTO9 (green) double-stained

    Journal: Environmental microbiology reports

    Article Title: The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa

    doi: 10.1111/1758-2229.12252

    Figure Lengend Snippet: The eDNA and dead bacteria in pellicles of P. aeruginosa PAO1 were stained by Propidium iodide (PI) to confirm that fibre-like DNA was eDNA. Shown were the optical sectioned images in the middle of pellicles. (A) PI (red) and SYTO9 (green) double-stained

    Article Snippet: Genomic DNAs of neutrophils, P. aeruginosa and S. aureus ATCC 6538 were purified with Wizard Genomic DNA Purification Kits (Promega).

    Techniques: Staining