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Transgenomic
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dna fragment analysis system - by Bioz Stars,
2021-04
86/100 stars
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Image Search Results

Journal: PLoS ONE
Article Title: Toll-Like Receptor 4 (TLR4) and Typhoid Fever in Vietnam
doi: 10.1371/journal.pone.0004800
Figure Lengend Snippet: Mutation detection in the T4_promoter fragment. Three polymorphisms were detected by dHPLC in the T4_promoter fragment and were verified by DNA sequencing. The four dHPLC graphs correspond to the wild-type sequence (A), SNPs T-441C (B), SNP A-271G (C) and SNP G-259C (D). The wild-type pattern is visible in all dHPLC graphs as a 1 peak trace. SNP T-441C is identified by an unequal height 2 peak trace (B), SNP A-271G is identified by an equal height 2 peak trace (C) and SNP G-259C is identified by a 3 peak trace (D). The positions of the sequence variants in the T4_promoter fragment identified by dHPLC were determined by DNA sequencing. All SNPs were present in the heterozygous state.
Article Snippet: Mutation Detection by dHPLC Primers and amplicons were designed to detect mutations in TLR4 by dHPLC according to the recommendations described by the manufacturer (
Techniques: Mutagenesis, DNA Sequencing, Sequencing