waters 616 liquid chromatography Search Results


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  • 85
    Waters Corporation waters 616 liquid chromatography
    Waters 616 Liquid Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation high performance liquid chromatography hplc system
    High Performance Liquid Chromatography Hplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation waters 616 626 hplc
    Waters 616 626 Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation waters 616 pump
    Waters 616 Pump, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation solvent delivery pump
    Solvent Delivery Pump, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation 616 hplc machine
    616 Hplc Machine, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation 600s hplc system
    600s Hplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation 616 996 pda detector hplc system
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    Waters Corporation 616 lc pump
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    Waters Corporation dual pump hplc
    Analyses of cytosine oxidation products. ( a ) <t>HPLC/EC</t> analysis of 5-hydroxycytosine ( 3) and 5-hydroxyuracil ( 5 ). Top chromatogram–standard compounds; middle chromatogram–freshly oxidized poly(dG-dC) subjected to acid hydrolysis; bottom chromatogram–heat treated oxidized poly(dG-dC) subjected to acid hydrolysis. Products 3 and 5 were detected by electrochemical detection with the oxidation potential at 75 mV and 350 mV, respectively. ( b ) GC/MS analysis of 5-hydroxycytosine ( 3) , 5-hydroxyuracil ( 5 ) and uracil glycols ( 4a and 4b ). The samples were prepared from polymers by either acid hydrolysis or incubation with Endo III followed by trimethylsilylation of the resulting <t>nucleobases.</t> The most abundant ion in the mass spectrum was chosen for selective ion monitoring (molecular ion −15 amu, unless indicated): 5-hydroxycytosine ( 3 , m/z 328); 5-hydroxyuracil ( 6 , m/z 329); trans uracil glycol ( 4a , m/z 245, ion fragment) and cis uracil glycol ( 4b , m/z 245, ion fragment). The peak at 27 min was an impurity. c) Quantitation of 5-hydroxycytosine ( 3 ), released from oxidized poly(dG-dC) by Endo III, was achieved by GC/MS analysis with selective ion monitoring. The amount of 5-hydroxycytosine was determined from the ratio of natural product released by Endo III to the corresponding isotopically labeled 5-hydroxycytosine (+3 amu), which was added before the addition of enzyme. The chromatogram depicts the excision of the 5-hydroxycytosine ( 3 ) from freshly oxidized poly(dG-dC) (left) and heat-treated polymer (right).
    Dual Pump Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation hplc uv method
    Effect of cyclodextrins on the solubility of <t>amiodarone</t> at a fixed concentration of 1.8 mg/mL. Molar ratio of amiodarone and cyclodextrins (water, pH 4.0–5.0) compared with the concentration of amiodarone measured by high pressure liquid chromatography ultraviolet <t>(HPLC-UV).</t> ●=sulfobutylether-beta-cyclodextrin. ▴=hydroxypropyl-beta-cyclodextrin. BCD, β-cyclodextrin.
    Hplc Uv Method, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analyses of cytosine oxidation products. ( a ) HPLC/EC analysis of 5-hydroxycytosine ( 3) and 5-hydroxyuracil ( 5 ). Top chromatogram–standard compounds; middle chromatogram–freshly oxidized poly(dG-dC) subjected to acid hydrolysis; bottom chromatogram–heat treated oxidized poly(dG-dC) subjected to acid hydrolysis. Products 3 and 5 were detected by electrochemical detection with the oxidation potential at 75 mV and 350 mV, respectively. ( b ) GC/MS analysis of 5-hydroxycytosine ( 3) , 5-hydroxyuracil ( 5 ) and uracil glycols ( 4a and 4b ). The samples were prepared from polymers by either acid hydrolysis or incubation with Endo III followed by trimethylsilylation of the resulting nucleobases. The most abundant ion in the mass spectrum was chosen for selective ion monitoring (molecular ion −15 amu, unless indicated): 5-hydroxycytosine ( 3 , m/z 328); 5-hydroxyuracil ( 6 , m/z 329); trans uracil glycol ( 4a , m/z 245, ion fragment) and cis uracil glycol ( 4b , m/z 245, ion fragment). The peak at 27 min was an impurity. c) Quantitation of 5-hydroxycytosine ( 3 ), released from oxidized poly(dG-dC) by Endo III, was achieved by GC/MS analysis with selective ion monitoring. The amount of 5-hydroxycytosine was determined from the ratio of natural product released by Endo III to the corresponding isotopically labeled 5-hydroxycytosine (+3 amu), which was added before the addition of enzyme. The chromatogram depicts the excision of the 5-hydroxycytosine ( 3 ) from freshly oxidized poly(dG-dC) (left) and heat-treated polymer (right).

    Journal: Nucleic Acids Research

    Article Title: Dehydration, deamination and enzymatic repair of cytosine glycols from oxidized poly(dG-dC) and poly(dI-dC)

    doi: 10.1093/nar/gkm1013

    Figure Lengend Snippet: Analyses of cytosine oxidation products. ( a ) HPLC/EC analysis of 5-hydroxycytosine ( 3) and 5-hydroxyuracil ( 5 ). Top chromatogram–standard compounds; middle chromatogram–freshly oxidized poly(dG-dC) subjected to acid hydrolysis; bottom chromatogram–heat treated oxidized poly(dG-dC) subjected to acid hydrolysis. Products 3 and 5 were detected by electrochemical detection with the oxidation potential at 75 mV and 350 mV, respectively. ( b ) GC/MS analysis of 5-hydroxycytosine ( 3) , 5-hydroxyuracil ( 5 ) and uracil glycols ( 4a and 4b ). The samples were prepared from polymers by either acid hydrolysis or incubation with Endo III followed by trimethylsilylation of the resulting nucleobases. The most abundant ion in the mass spectrum was chosen for selective ion monitoring (molecular ion −15 amu, unless indicated): 5-hydroxycytosine ( 3 , m/z 328); 5-hydroxyuracil ( 6 , m/z 329); trans uracil glycol ( 4a , m/z 245, ion fragment) and cis uracil glycol ( 4b , m/z 245, ion fragment). The peak at 27 min was an impurity. c) Quantitation of 5-hydroxycytosine ( 3 ), released from oxidized poly(dG-dC) by Endo III, was achieved by GC/MS analysis with selective ion monitoring. The amount of 5-hydroxycytosine was determined from the ratio of natural product released by Endo III to the corresponding isotopically labeled 5-hydroxycytosine (+3 amu), which was added before the addition of enzyme. The chromatogram depicts the excision of the 5-hydroxycytosine ( 3 ) from freshly oxidized poly(dG-dC) (left) and heat-treated polymer (right).

    Article Snippet: HPLC analysis of modified nucleobases was performed using a dual pump HPLC (Model 616, Waters) with a solvent controller (Model 600S, Waters), attached to an automated injector (Model 717 plus, Waters), PDA detector (Model 996, Waters) and an electrochemical detector (Coulochem II Model 5200, ESA Associates) equipped with an electrochemical cell (Model 5011, ESA).

    Techniques: High Performance Liquid Chromatography, Gas Chromatography-Mass Spectrometry, Incubation, Quantitation Assay, Labeling

    Effect of cyclodextrins on the solubility of amiodarone at a fixed concentration of 1.8 mg/mL. Molar ratio of amiodarone and cyclodextrins (water, pH 4.0–5.0) compared with the concentration of amiodarone measured by high pressure liquid chromatography ultraviolet (HPLC-UV). ●=sulfobutylether-beta-cyclodextrin. ▴=hydroxypropyl-beta-cyclodextrin. BCD, β-cyclodextrin.

    Journal: European Journal of Hospital Pharmacy

    Article Title: Ready-to-use parenteral amiodarone: a feasibility study towards a long-term stable product formulation

    doi: 10.1136/ejhpharm-2015-000860

    Figure Lengend Snippet: Effect of cyclodextrins on the solubility of amiodarone at a fixed concentration of 1.8 mg/mL. Molar ratio of amiodarone and cyclodextrins (water, pH 4.0–5.0) compared with the concentration of amiodarone measured by high pressure liquid chromatography ultraviolet (HPLC-UV). ●=sulfobutylether-beta-cyclodextrin. ▴=hydroxypropyl-beta-cyclodextrin. BCD, β-cyclodextrin.

    Article Snippet: The amiodarone amount in the final product was determined by a validated HPLC-UV method (Waters 600S, Waters 616 Pump).

    Techniques: Solubility, Concentration Assay, High Performance Liquid Chromatography