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    New England Biolabs warmstart rtx reverse transcriptase
    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with <t>NEB’s</t> <t>RTx</t> reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).
    Warmstart Rtx Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warmstart rtx reverse transcriptase/product/New England Biolabs
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    warmstart rtx reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars
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    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Journal: bioRxiv

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing

    doi: 10.1101/2020.06.23.166397

    Figure Lengend Snippet: A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Article Snippet: dUTP/UDG contamination prevention system Reactions were set up to contain NEB 1x Isothermal Amplification Buffer, 1.4 mM of each dATP, dCTP, dGTP, 0.7 mM dUTP, 0.7 mM dTTP, 6 mM MgSO4 (100 mM stock, NEB), 0.32 U/µ l NEB Bst 2.0 polymerase, 0.3 U/µ l NEB Warmstart RTx Reverse Transcriptase, 0.2 U/µ l NEB Antarctic thermolabile UDG, sample and nuclease-free water.

    Techniques: RT Lamp Assay, Amplification