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    New England Biolabs mlu i
    Mlu I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs ultra dna library prep kit
    Ultra Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs q5 high fidelity dna polymerase
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    New England Biolabs restriction endonuclease bci vi
    Agarose gel electrophoresis of the V L <t>PCR</t> product before and after <t>Bci</t> VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.
    Restriction Endonuclease Bci Vi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Agarose gel electrophoresis of the V L PCR product before and after Bci VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.

    Journal: Cellular and Molecular Immunology

    Article Title: Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes

    doi: 10.1038/cmi.2010.33

    Figure Lengend Snippet: Agarose gel electrophoresis of the V L PCR product before and after Bci VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.

    Article Snippet: Therefore, the PCR product of VL was digested at 37 °C overnight by the restriction endonuclease Bci VI (New England Biolabs, Ipswich, MA, USA) and only non-cut fragments were gel extracted prior to ligation to a pGEM-T Easy vector.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Purification, Functional Assay, Incubation