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    New England Biolabs mlu i
    Mlu I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nebnext ultra dna library prep kit for illumina
    Nebnext Ultra Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs q5 high fidelity dna polymerase
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs vl gene
    Vl Gene, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs restriction endonuclease bci vi
    Agarose gel electrophoresis of the V L <t>PCR</t> product before and after <t>Bci</t> VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.
    Restriction Endonuclease Bci Vi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Agarose gel electrophoresis of the V L PCR product before and after Bci VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.

    Journal: Cellular and Molecular Immunology

    Article Title: Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes

    doi: 10.1038/cmi.2010.33

    Figure Lengend Snippet: Agarose gel electrophoresis of the V L PCR product before and after Bci VI digestion. Purified PCR product of the V L gene was digested by Bci VI to isolate the functional V L gene. After incubation overnight at 37 °C, most of the product was cut to form two bands of approximately 180 and 190 bp. Non-cut products were rare.

    Article Snippet: Therefore, the PCR product of VL was digested at 37 °C overnight by the restriction endonuclease Bci VI (New England Biolabs, Ipswich, MA, USA) and only non-cut fragments were gel extracted prior to ligation to a pGEM-T Easy vector.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Purification, Functional Assay, Incubation