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  • 99
    Zymo Research viral rna kit
    NHC has stronger negative effects on the release and infectivity of VEEV TC-83 and PREV1 particles than those of the PP2 mutant. A total of 5 × 10 5 Vero cells in six-well plates were infected with VEEV TC-83, PP2, or PREV1 at an MOI of 1 PFU/cell and were incubated with or without 2 μM NHC. At the indicated time points, media were collected, and the infectious titers (PFU/ml) and concentrations of genome-containing viral particles (GE/ml) were determined by a plaque assay on Vero cells and RT-qPCR, respectively. Experiments were performed in triplicates. The standard deviations are too small to be visible on the plots. (B) GE/PFU ratios in virus samples harvested at 24 h p.i. of mock-treated and NHC-treated cells in the experiment presented in panel A. (C) Levels of viral G <t>RNAs</t> in the mock-treated cells at 24 h p.i. The cells were harvested after collecting the medium samples described in panel A at 24 h p.i. Data were normalized to the G <t>RNA</t> levels in VEEV TC-83-infected cells. (D) Ratio of G RNAs in mock-treated versus NHC-treated cells at 24 p.i. with the indicated viruses. Cells were collected in the experiment presented in panel A.
    Viral Rna Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral rna kit/product/Zymo Research
    Average 99 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    viral rna kit - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Thermo Fisher magmax viral rna kit
    NHC has stronger negative effects on the release and infectivity of VEEV TC-83 and PREV1 particles than those of the PP2 mutant. A total of 5 × 10 5 Vero cells in six-well plates were infected with VEEV TC-83, PP2, or PREV1 at an MOI of 1 PFU/cell and were incubated with or without 2 μM NHC. At the indicated time points, media were collected, and the infectious titers (PFU/ml) and concentrations of genome-containing viral particles (GE/ml) were determined by a plaque assay on Vero cells and RT-qPCR, respectively. Experiments were performed in triplicates. The standard deviations are too small to be visible on the plots. (B) GE/PFU ratios in virus samples harvested at 24 h p.i. of mock-treated and NHC-treated cells in the experiment presented in panel A. (C) Levels of viral G <t>RNAs</t> in the mock-treated cells at 24 h p.i. The cells were harvested after collecting the medium samples described in panel A at 24 h p.i. Data were normalized to the G <t>RNA</t> levels in VEEV TC-83-infected cells. (D) Ratio of G RNAs in mock-treated versus NHC-treated cells at 24 p.i. with the indicated viruses. Cells were collected in the experiment presented in panel A.
    Magmax Viral Rna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magmax viral rna kit/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    magmax viral rna kit - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Qiagen viral rna
    NHC has stronger negative effects on the release and infectivity of VEEV TC-83 and PREV1 particles than those of the PP2 mutant. A total of 5 × 10 5 Vero cells in six-well plates were infected with VEEV TC-83, PP2, or PREV1 at an MOI of 1 PFU/cell and were incubated with or without 2 μM NHC. At the indicated time points, media were collected, and the infectious titers (PFU/ml) and concentrations of genome-containing viral particles (GE/ml) were determined by a plaque assay on Vero cells and RT-qPCR, respectively. Experiments were performed in triplicates. The standard deviations are too small to be visible on the plots. (B) GE/PFU ratios in virus samples harvested at 24 h p.i. of mock-treated and NHC-treated cells in the experiment presented in panel A. (C) Levels of viral G <t>RNAs</t> in the mock-treated cells at 24 h p.i. The cells were harvested after collecting the medium samples described in panel A at 24 h p.i. Data were normalized to the G <t>RNA</t> levels in VEEV TC-83-infected cells. (D) Ratio of G RNAs in mock-treated versus NHC-treated cells at 24 p.i. with the indicated viruses. Cells were collected in the experiment presented in panel A.
    Viral Rna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral rna/product/Qiagen
    Average 99 stars, based on 18888 article reviews
    Price from $9.99 to $1999.99
    viral rna - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Qiagen qiaamp viral rna
    Influenza A virus replication is dependent upon opposite virus-induced effects on Bax and Bak activity that are unlikely to be interferon related. (A) Influenza A virus replication was analyzed by plaque assay. Virus replication is severely attenuated in Bax KO cells, resulting in a 2-log decrease in PFU/ml compared to the WT. Bak KO cells allow a maximum replication similar to that of the WT, while Bax/Bak DKO cells show a slight elevation of infectious titers during infection. These results indicate that Bax is proviral during infection, while Bak is dispensable for replication. (B) Bax was transiently expressed in all cell types by Lipofectamine 2000 transfection of a C2-Bax-GFP construct prior to infection, and supernatant samples were collected for plaque assay at 48 <t>hpi.</t> Baseline virus replication in each cell type was evaluated using empty C2-GFP plasmid transfection. Bax reconstitution in Bax KO cells resulted in a fivefold increase in infectious titers compared to the control ( P = 0.0007). A minimal effect on the virus titer was seen after Bax overexpression by transient transfection in WT cells compared to empty plasmid controls. (C) Influenza A virus replication was assessed by reverse transcription-PCR. Serial dilutions of stock virus at known concentrations were also analyzed to generate a standard curve to which experimental samples were compared, thus calculating the approximate number of influenza A virus particles/ml in each sample. By 24 hpi, Bax KO, Bak KO, and Bax/Bak DKO cells all showed significantly higher levels of influenza A virus <t>RNA</t> released into the cell culture supernatant than did WT cells. (D) Interferon activity was assessed by infecting mock- and influenza A virus-infected cells with interferon-sensitive, GFP-linked NDV and quantifying the mean GFP expression levels of 10,000 events per condition by FACS analysis. Each assay was run in triplicate, and data are expressed as the ratio of the numbers of influenza A virus-infected to mock-infected cells per cell type. After influenza A virus infection, Bak KO cells exhibited a slight decrease in ratio compared to the WT, representing a 30% increase in interferon activity ( P = 0.002). Bax KO and Bax/Bak DKO cells both showed similar fluorescence changes compared to the WT after infection. Due the high degree of similarity between cell types, these results suggest that the interferon response in infected cells is modulated by viral replication in the presence of Bak and is only slightly modified by Bax activity during influenza A virus infection. As an elevated interferon response typically leads to a reduced virus replication capacity, these results also suggest that it is unlikely that the observed trends in infectious virus titer are due to virus-induced interferon signaling.
    Qiaamp Viral Rna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp viral rna/product/Qiagen
    Average 99 stars, based on 645 article reviews
    Price from $9.99 to $1999.99
    qiaamp viral rna - by Bioz Stars, 2020-07
    99/100 stars
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    96
    Zymo Research viral rna
    Accumulation of viral genomes and transcripts in infected HMVEC. (A) Total <t>DNA</t> was isolated from HMVEC infected with TB40/E-WT (filled bars), - UL135 STOP (shaded bars), or - UL136 GalK (open bars) (MOI, 0.1) at the time points indicated. Genomes were quantified by real-time PCR using primers specific to UL99, and values were normalized to those for the cellular gene RNaseP. The results of one experiment representative of three total experiments are shown. (B to D) Transcripts for UL123/IE1 (B), UL69 (C), and UL32/pp150 (D) were quantified from cDNA synthesized from total <t>RNA</t> isolated from infected HMVEC over a time course. Transcript levels were normalized to those for β-actin. The bars represent average values from three independent experiments. ΔCT, change in threshold cycle.
    Viral Rna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral rna/product/Zymo Research
    Average 96 stars, based on 407 article reviews
    Price from $9.99 to $1999.99
    viral rna - by Bioz Stars, 2020-07
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    94
    Qiagen qiaamp viral rna minikit
    Comparison of viral recovery in various tissue types stored in <t>RNA</t> later ™ from a CDV outbreak in Vienna, Austria. Data represents the average of testing a set of tissues from 3 separate animals (n = 3): a CDV positive pine martin, red fox and European badger. Standard deviations are shown, except in small intestine (* indicates tissue where only two cases were available for analysis). A. Comparison of Ct values between the Biomeme M1 Sample Prep Kit ™ and <t>QIAamp</t> ® Viral RNA extraction kit from tissue swabs. P-values are shown in samples evaluated by a two-tailed student t-test. B. Average Ct differences between Biomeme and Qiagen RNA extraction methods for each tissue swab type (in A). C. Average calculated copy number recovery in total RNA extracts. P-values are shown in samples evaluated by a two-tailed student t-test (p > 0.07).
    Qiaamp Viral Rna Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 4902 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp viral rna minikit/product/Qiagen
    Average 94 stars, based on 4902 article reviews
    Price from $9.99 to $1999.99
    qiaamp viral rna minikit - by Bioz Stars, 2020-07
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    92
    Omega Bio-tek viral rna kit
    CSGalNAcT2 is up-regulated during <t>IBDV</t> infection. ( a ) Differential gene expression analysis by gene chip assay. The total <t>RNA</t> of CEF cells infected by IBDV Gt strain at 24 h post-infection was extracted for gene chip analysis. The value of transcripts per million clean reads (TPM) of CSGalNAcT2 showed that CSGalNAcT2 was up-regulated after IBDV infection. We set the false discovery rates (FDR)
    Viral Rna Kit, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 92/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral rna kit/product/Omega Bio-tek
    Average 92 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    viral rna kit - by Bioz Stars, 2020-07
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    Image Search Results


    NHC has stronger negative effects on the release and infectivity of VEEV TC-83 and PREV1 particles than those of the PP2 mutant. A total of 5 × 10 5 Vero cells in six-well plates were infected with VEEV TC-83, PP2, or PREV1 at an MOI of 1 PFU/cell and were incubated with or without 2 μM NHC. At the indicated time points, media were collected, and the infectious titers (PFU/ml) and concentrations of genome-containing viral particles (GE/ml) were determined by a plaque assay on Vero cells and RT-qPCR, respectively. Experiments were performed in triplicates. The standard deviations are too small to be visible on the plots. (B) GE/PFU ratios in virus samples harvested at 24 h p.i. of mock-treated and NHC-treated cells in the experiment presented in panel A. (C) Levels of viral G RNAs in the mock-treated cells at 24 h p.i. The cells were harvested after collecting the medium samples described in panel A at 24 h p.i. Data were normalized to the G RNA levels in VEEV TC-83-infected cells. (D) Ratio of G RNAs in mock-treated versus NHC-treated cells at 24 p.i. with the indicated viruses. Cells were collected in the experiment presented in panel A.

    Journal: Journal of Virology

    Article Title: β-d-N4-Hydroxycytidine Is a Potent Anti-alphavirus Compound That Induces a High Level of Mutations in the Viral Genome

    doi: 10.1128/JVI.01965-17

    Figure Lengend Snippet: NHC has stronger negative effects on the release and infectivity of VEEV TC-83 and PREV1 particles than those of the PP2 mutant. A total of 5 × 10 5 Vero cells in six-well plates were infected with VEEV TC-83, PP2, or PREV1 at an MOI of 1 PFU/cell and were incubated with or without 2 μM NHC. At the indicated time points, media were collected, and the infectious titers (PFU/ml) and concentrations of genome-containing viral particles (GE/ml) were determined by a plaque assay on Vero cells and RT-qPCR, respectively. Experiments were performed in triplicates. The standard deviations are too small to be visible on the plots. (B) GE/PFU ratios in virus samples harvested at 24 h p.i. of mock-treated and NHC-treated cells in the experiment presented in panel A. (C) Levels of viral G RNAs in the mock-treated cells at 24 h p.i. The cells were harvested after collecting the medium samples described in panel A at 24 h p.i. Data were normalized to the G RNA levels in VEEV TC-83-infected cells. (D) Ratio of G RNAs in mock-treated versus NHC-treated cells at 24 p.i. with the indicated viruses. Cells were collected in the experiment presented in panel A.

    Article Snippet: Total cellular RNAs were isolated from infected Vero cells using a ZR viral RNA kit. cDNAs synthesis and qPCR analyses were performed using the above-described protocols, and the data were normalized to the concentrations of 18S rRNA present in the same samples.

    Techniques: Infection, Mutagenesis, Incubation, Plaque Assay, Quantitative RT-PCR

    Results of the RNase I protection assay for MNV after exposure to a concentration of 4.0% of lemongrass oil (A), citral (B), or allspice oil (C). The log 10 genome copy numbers of MNV RNA recovered were determined by RT-qPCR after exposure to each antimicrobial

    Journal: Applied and Environmental Microbiology

    Article Title: Mechanisms of Antiviral Action of Plant Antimicrobials against Murine Norovirus

    doi: 10.1128/AEM.00402-14

    Figure Lengend Snippet: Results of the RNase I protection assay for MNV after exposure to a concentration of 4.0% of lemongrass oil (A), citral (B), or allspice oil (C). The log 10 genome copy numbers of MNV RNA recovered were determined by RT-qPCR after exposure to each antimicrobial

    Article Snippet: The viral RNA (from both MNV and PV1) was purified from each sample using the ZR viral RNA kits according to the manufacturer's protocol, with the exception that the final volume was adjusted to 20 μl.

    Techniques: Concentration Assay, Quantitative RT-PCR

    Influenza A virus replication is dependent upon opposite virus-induced effects on Bax and Bak activity that are unlikely to be interferon related. (A) Influenza A virus replication was analyzed by plaque assay. Virus replication is severely attenuated in Bax KO cells, resulting in a 2-log decrease in PFU/ml compared to the WT. Bak KO cells allow a maximum replication similar to that of the WT, while Bax/Bak DKO cells show a slight elevation of infectious titers during infection. These results indicate that Bax is proviral during infection, while Bak is dispensable for replication. (B) Bax was transiently expressed in all cell types by Lipofectamine 2000 transfection of a C2-Bax-GFP construct prior to infection, and supernatant samples were collected for plaque assay at 48 hpi. Baseline virus replication in each cell type was evaluated using empty C2-GFP plasmid transfection. Bax reconstitution in Bax KO cells resulted in a fivefold increase in infectious titers compared to the control ( P = 0.0007). A minimal effect on the virus titer was seen after Bax overexpression by transient transfection in WT cells compared to empty plasmid controls. (C) Influenza A virus replication was assessed by reverse transcription-PCR. Serial dilutions of stock virus at known concentrations were also analyzed to generate a standard curve to which experimental samples were compared, thus calculating the approximate number of influenza A virus particles/ml in each sample. By 24 hpi, Bax KO, Bak KO, and Bax/Bak DKO cells all showed significantly higher levels of influenza A virus RNA released into the cell culture supernatant than did WT cells. (D) Interferon activity was assessed by infecting mock- and influenza A virus-infected cells with interferon-sensitive, GFP-linked NDV and quantifying the mean GFP expression levels of 10,000 events per condition by FACS analysis. Each assay was run in triplicate, and data are expressed as the ratio of the numbers of influenza A virus-infected to mock-infected cells per cell type. After influenza A virus infection, Bak KO cells exhibited a slight decrease in ratio compared to the WT, representing a 30% increase in interferon activity ( P = 0.002). Bax KO and Bax/Bak DKO cells both showed similar fluorescence changes compared to the WT after infection. Due the high degree of similarity between cell types, these results suggest that the interferon response in infected cells is modulated by viral replication in the presence of Bak and is only slightly modified by Bax activity during influenza A virus infection. As an elevated interferon response typically leads to a reduced virus replication capacity, these results also suggest that it is unlikely that the observed trends in infectious virus titer are due to virus-induced interferon signaling.

    Journal: Journal of Virology

    Article Title: Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication ▿

    doi: 10.1128/JVI.02672-08

    Figure Lengend Snippet: Influenza A virus replication is dependent upon opposite virus-induced effects on Bax and Bak activity that are unlikely to be interferon related. (A) Influenza A virus replication was analyzed by plaque assay. Virus replication is severely attenuated in Bax KO cells, resulting in a 2-log decrease in PFU/ml compared to the WT. Bak KO cells allow a maximum replication similar to that of the WT, while Bax/Bak DKO cells show a slight elevation of infectious titers during infection. These results indicate that Bax is proviral during infection, while Bak is dispensable for replication. (B) Bax was transiently expressed in all cell types by Lipofectamine 2000 transfection of a C2-Bax-GFP construct prior to infection, and supernatant samples were collected for plaque assay at 48 hpi. Baseline virus replication in each cell type was evaluated using empty C2-GFP plasmid transfection. Bax reconstitution in Bax KO cells resulted in a fivefold increase in infectious titers compared to the control ( P = 0.0007). A minimal effect on the virus titer was seen after Bax overexpression by transient transfection in WT cells compared to empty plasmid controls. (C) Influenza A virus replication was assessed by reverse transcription-PCR. Serial dilutions of stock virus at known concentrations were also analyzed to generate a standard curve to which experimental samples were compared, thus calculating the approximate number of influenza A virus particles/ml in each sample. By 24 hpi, Bax KO, Bak KO, and Bax/Bak DKO cells all showed significantly higher levels of influenza A virus RNA released into the cell culture supernatant than did WT cells. (D) Interferon activity was assessed by infecting mock- and influenza A virus-infected cells with interferon-sensitive, GFP-linked NDV and quantifying the mean GFP expression levels of 10,000 events per condition by FACS analysis. Each assay was run in triplicate, and data are expressed as the ratio of the numbers of influenza A virus-infected to mock-infected cells per cell type. After influenza A virus infection, Bak KO cells exhibited a slight decrease in ratio compared to the WT, representing a 30% increase in interferon activity ( P = 0.002). Bax KO and Bax/Bak DKO cells both showed similar fluorescence changes compared to the WT after infection. Due the high degree of similarity between cell types, these results suggest that the interferon response in infected cells is modulated by viral replication in the presence of Bak and is only slightly modified by Bax activity during influenza A virus infection. As an elevated interferon response typically leads to a reduced virus replication capacity, these results also suggest that it is unlikely that the observed trends in infectious virus titer are due to virus-induced interferon signaling.

    Article Snippet: Total viral RNA was extracted at 12 hpi and 24 hpi using a viral RNA extraction kit (catalog no. 52904; Qiagen) according to the manufacturer's protocol. cDNA was then generated from extracted viral RNA using a Superscript III first-strand synthesis kit (catalog no. 18080-400; Invitrogen) according to the manufacturer's protocol.

    Techniques: Activity Assay, Plaque Assay, Infection, Transfection, Construct, Plasmid Preparation, Over Expression, Polymerase Chain Reaction, Cell Culture, Expressing, FACS, Fluorescence, Modification

    Comparing the DRM in RNA (plasma) and DNA (peripheral blood mononuclear cells). a NRTI. b NNRTI

    Journal: AIDS Research and Therapy

    Article Title: High level of HIV-1 drug resistance mutations in patients with unsuppressed viral loads in rural northern South Africa

    doi: 10.1186/s12981-017-0161-z

    Figure Lengend Snippet: Comparing the DRM in RNA (plasma) and DNA (peripheral blood mononuclear cells). a NRTI. b NNRTI

    Article Snippet: DNA and RNA extractions Plasma RNA and proviral DNA from total cells were extracted using Qiagen Viral RNA Mini Kit and Qiagen blood DNA Midi Kit (Qiagen, Valencia, CA, USA), respectively, according to the manufacturers’ protocols.

    Techniques:

    Accumulation of viral genomes and transcripts in infected HMVEC. (A) Total DNA was isolated from HMVEC infected with TB40/E-WT (filled bars), - UL135 STOP (shaded bars), or - UL136 GalK (open bars) (MOI, 0.1) at the time points indicated. Genomes were quantified by real-time PCR using primers specific to UL99, and values were normalized to those for the cellular gene RNaseP. The results of one experiment representative of three total experiments are shown. (B to D) Transcripts for UL123/IE1 (B), UL69 (C), and UL32/pp150 (D) were quantified from cDNA synthesized from total RNA isolated from infected HMVEC over a time course. Transcript levels were normalized to those for β-actin. The bars represent average values from three independent experiments. ΔCT, change in threshold cycle.

    Journal: Journal of Virology

    Article Title: Human Cytomegalovirus UL135 and UL136 Genes Are Required for Postentry Tropism in Endothelial Cells

    doi: 10.1128/JVI.00284-15

    Figure Lengend Snippet: Accumulation of viral genomes and transcripts in infected HMVEC. (A) Total DNA was isolated from HMVEC infected with TB40/E-WT (filled bars), - UL135 STOP (shaded bars), or - UL136 GalK (open bars) (MOI, 0.1) at the time points indicated. Genomes were quantified by real-time PCR using primers specific to UL99, and values were normalized to those for the cellular gene RNaseP. The results of one experiment representative of three total experiments are shown. (B to D) Transcripts for UL123/IE1 (B), UL69 (C), and UL32/pp150 (D) were quantified from cDNA synthesized from total RNA isolated from infected HMVEC over a time course. Transcript levels were normalized to those for β-actin. The bars represent average values from three independent experiments. ΔCT, change in threshold cycle.

    Article Snippet: Total viral DNA and viral RNA were isolated from infected cells by using the Zymo Duet kit with on-column DNase digestion for RNA samples according to the manufacturers' instructions (Zymo Research, Irvine, CA, and Macherey-Nagel, Inc., Bethlehem, PA). cDNA was synthesized using a Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN).

    Techniques: Infection, Isolation, Real-time Polymerase Chain Reaction, Synthesized

    Comparison of viral recovery in various tissue types stored in RNA later ™ from a CDV outbreak in Vienna, Austria. Data represents the average of testing a set of tissues from 3 separate animals (n = 3): a CDV positive pine martin, red fox and European badger. Standard deviations are shown, except in small intestine (* indicates tissue where only two cases were available for analysis). A. Comparison of Ct values between the Biomeme M1 Sample Prep Kit ™ and QIAamp ® Viral RNA extraction kit from tissue swabs. P-values are shown in samples evaluated by a two-tailed student t-test. B. Average Ct differences between Biomeme and Qiagen RNA extraction methods for each tissue swab type (in A). C. Average calculated copy number recovery in total RNA extracts. P-values are shown in samples evaluated by a two-tailed student t-test (p > 0.07).

    Journal: PLoS ONE

    Article Title: Development and validation of a portable, point-of-care canine distemper virus qPCR test

    doi: 10.1371/journal.pone.0232044

    Figure Lengend Snippet: Comparison of viral recovery in various tissue types stored in RNA later ™ from a CDV outbreak in Vienna, Austria. Data represents the average of testing a set of tissues from 3 separate animals (n = 3): a CDV positive pine martin, red fox and European badger. Standard deviations are shown, except in small intestine (* indicates tissue where only two cases were available for analysis). A. Comparison of Ct values between the Biomeme M1 Sample Prep Kit ™ and QIAamp ® Viral RNA extraction kit from tissue swabs. P-values are shown in samples evaluated by a two-tailed student t-test. B. Average Ct differences between Biomeme and Qiagen RNA extraction methods for each tissue swab type (in A). C. Average calculated copy number recovery in total RNA extracts. P-values are shown in samples evaluated by a two-tailed student t-test (p > 0.07).

    Article Snippet: This included: 1. comparison between the Biomeme M1 Sample Prep Kit™ and the Qiagen QIAamp® Viral RNA minikit for RNA extraction and CDV detection in a dilution series of DA2PP vaccine, 2. comparison of the Biomeme LyoRNA™ RT-PCR mastermix and the Qiagen QuantiFast Pathogen RT-PCR mastermix for CDV detection using a synthetic CDV plasmid positive control, 3. comparison of the Biomeme two3™ and Bio-Rad Mini-Opticon qPCR thermocycler for CDV detection with synthetic plasmid positive control, 4. comparison of RNA extraction between the Biomeme M1 Sample Prep Kit™ and the Qiagen QiAMP Viral RNA minikit for CDV detection with CDV-suspect, wild animal samples preserved in RNAlater ™ , 5. comparison of the Biomeme M1 Sample Prep Kit™ in combination with the Biomeme LyoRNA™ RT-PCR mastermix to the Qiagen kits (RNA extraction and mastermix) for CDV detection from fresh tissues and nasal swabs from CDV-suspect, wild raccoons, and finally, 6. comparison of test performance and results for CDV detection in samples from CDV-suspect, wild animals using the complete Biomeme platform (M1 Sample Prep Kit™ , CDV Go-Strips™ , and Biomeme two3™ thermocycler) or standard laboratory equipment and methods at an independent virology laboratory in Austria.

    Techniques: Sample Prep, RNA Extraction, Two Tailed Test

    CSGalNAcT2 is up-regulated during IBDV infection. ( a ) Differential gene expression analysis by gene chip assay. The total RNA of CEF cells infected by IBDV Gt strain at 24 h post-infection was extracted for gene chip analysis. The value of transcripts per million clean reads (TPM) of CSGalNAcT2 showed that CSGalNAcT2 was up-regulated after IBDV infection. We set the false discovery rates (FDR)

    Journal: Viruses

    Article Title: Chondroitin Sulfate N-acetylgalactosaminyltransferase-2 Contributes to the Replication of Infectious Bursal Disease Virus via Interaction with the Capsid Protein VP2

    doi: 10.3390/v7031474

    Figure Lengend Snippet: CSGalNAcT2 is up-regulated during IBDV infection. ( a ) Differential gene expression analysis by gene chip assay. The total RNA of CEF cells infected by IBDV Gt strain at 24 h post-infection was extracted for gene chip analysis. The value of transcripts per million clean reads (TPM) of CSGalNAcT2 showed that CSGalNAcT2 was up-regulated after IBDV infection. We set the false discovery rates (FDR)

    Article Snippet: Viral RNA Isolation and Real-Time RT-PCR Analysis To determine the genomic RNA copies of IBDV, the viral RNA was extracted from cell supernatants with the Viral RNA kit (Omega Biotek, Norcross, GA, USA) and then reverse transcribed into cDNAs with M-MLV reverse transcriptase (Invitrogen).

    Techniques: Infection, Expressing, Chromatin Immunoprecipitation