vi - r telomere Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs ecor v
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Ecor V, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v/product/New England Biolabs
    Average 99 stars, based on 143 article reviews
    Price from $9.99 to $1999.99
    ecor v - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher dnase i treated
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Dnase I Treated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i treated/product/Thermo Fisher
    Average 99 stars, based on 933 article reviews
    Price from $9.99 to $1999.99
    dnase i treated - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad icycler iq real time pcr detection system
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Icycler Iq Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icycler iq real time pcr detection system/product/Bio-Rad
    Average 99 stars, based on 9356 article reviews
    Price from $9.99 to $1999.99
    icycler iq real time pcr detection system - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs phusion high fidelity dna polymerase
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 24256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 24256 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity dna polymerase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs taq
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq/product/New England Biolabs
    Average 99 stars, based on 1644 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad real time pcr
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr/product/Bio-Rad
    Average 99 stars, based on 12747 article reviews
    Price from $9.99 to $1999.99
    real time pcr - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad iq sybr green supermix
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 72789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iq sybr green supermix/product/Bio-Rad
    Average 99 stars, based on 72789 article reviews
    Price from $9.99 to $1999.99
    iq sybr green supermix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare imagequant software
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Imagequant Software, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 14072 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagequant software/product/GE Healthcare
    Average 92 stars, based on 14072 article reviews
    Price from $9.99 to $1999.99
    imagequant software - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Difco ypd rich medium
    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic <t>DNA</t> from each PD was digested with <t>EcoR</t> V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )
    Ypd Rich Medium, supplied by Difco, used in various techniques. Bioz Stars score: 88/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ypd rich medium/product/Difco
    Average 88 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    ypd rich medium - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Qiagen pcr cloning kit
    <t>Telomere</t> length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R <t>PCR</t> after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.
    Pcr Cloning Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cloning kit/product/Qiagen
    Average 99 stars, based on 2012 article reviews
    Price from $9.99 to $1999.99
    pcr cloning kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher dnase free kit
    <t>Telomere</t> length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R <t>PCR</t> after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.
    Dnase Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free kit/product/Thermo Fisher
    Average 89 stars, based on 259 article reviews
    Price from $9.99 to $1999.99
    dnase free kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    99
    New England Biolabs q5 high fidelity dna polymerase
    <t>Telomere</t> length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R <t>PCR</t> after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 9046 article reviews
    Price from $9.99 to $1999.99
    q5 high fidelity dna polymerase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher total rna
    <t>Telomere</t> length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R <t>PCR</t> after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rna/product/Thermo Fisher
    Average 99 stars, based on 471882 article reviews
    Price from $9.99 to $1999.99
    total rna - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Becton Dickinson tlc1 bd
    <t>Telomere</t> length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R <t>PCR</t> after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.
    Tlc1 Bd, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlc1 bd/product/Becton Dickinson
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    tlc1 bd - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    94
    Promega wizard genomic dna purification kit
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 32734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna purification kit/product/Promega
    Average 94 stars, based on 32734 article reviews
    Price from $9.99 to $1999.99
    wizard genomic dna purification kit - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Bio-Rad cfx96 real time cycler
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Cfx96 Real Time Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx96 real time cycler/product/Bio-Rad
    Average 88 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    cfx96 real time cycler - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Qiagen qiaquick gel extraction kit
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 113321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaquick gel extraction kit/product/Qiagen
    Average 99 stars, based on 113321 article reviews
    Price from $9.99 to $1999.99
    qiaquick gel extraction kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    98
    Thermo Fisher zero blunt pcr cloning kit
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Zero Blunt Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zero blunt pcr cloning kit/product/Thermo Fisher
    Average 98 stars, based on 2055 article reviews
    Price from $9.99 to $1999.99
    zero blunt pcr cloning kit - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    99
    Millipore perfecthyb
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Perfecthyb, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perfecthyb/product/Millipore
    Average 99 stars, based on 138 article reviews
    Price from $9.99 to $1999.99
    perfecthyb - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore anti nestin
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Anti Nestin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nestin/product/Millipore
    Average 99 stars, based on 1843 article reviews
    Price from $9.99 to $1999.99
    anti nestin - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    89
    GE Healthcare vistra green
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Vistra Green, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vistra green/product/GE Healthcare
    Average 89 stars, based on 330 article reviews
    Price from $9.99 to $1999.99
    vistra green - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    94
    GE Healthcare protein g sepharose beads
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Protein G Sepharose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 12046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein g sepharose beads/product/GE Healthcare
    Average 94 stars, based on 12046 article reviews
    Price from $9.99 to $1999.99
    protein g sepharose beads - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs nebuffer 4
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Nebuffer 4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 4/product/New England Biolabs
    Average 99 stars, based on 682 article reviews
    Price from $9.99 to $1999.99
    nebuffer 4 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher zero blunt top o pcr cloning kit
    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A <t>telomere</t> genomic blot was performed on genomic <t>DNA</t> from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.
    Zero Blunt Top O Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zero blunt top o pcr cloning kit/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    zero blunt top o pcr cloning kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic DNA from each PD was digested with EcoR V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )

    Journal: Chromosoma

    Article Title: Mec1p associates with functionally compromised telomeres

    doi: 10.1007/s00412-011-0359-0

    Figure Lengend Snippet: Mec1-HAp associates with telomeres as cells escape the cell cycle arrest that occurs due to telomere shortening. a Telomere shortening in Mec1-HAp cells lacking the telomerase catalytic subunit Est2p. Cells lacking telomerase were generated by deleting the genomic copy of EST2 , covering the deletion with a plasmid bearing the EST2 gene, and then isolating cells that had lost the plasmid. Cells lacking telomerase were then cultured for the number of population doublings (PD) shown, where the 0 PD sample contains cells that retain the EST2 plasmid and the other PD samples contain cells that lack telomerase. Genomic DNA from each PD was digested with EcoR V and analyzed by Southern blotting using the VI -R PCR product from the primers shown in panel b as probe. Telomeres gradually shortened with increasing PD, allowing the isolation of cells with different telomere lengths. The black arrow designates a background band that hybridizes to probe. The white arrowheads designate rearranged telomeres in PD 70 that are characteristic of the survivor cells that escape the short telomere cell cycle arrest. b A schematic of the loci and primers used to monitor telomere association. The telomeres from the right arm of chromosome VI ( VI -R) and two internal loci from separate chromosomes ( GAL10 and ARO1 ) were PCR amplified in the ChIP experiments in this study. c Analysis of the Mec1-HAp immunoprecipitates using the PCR primers indicated in panel b . Telomere enrichment values are calculated as the ratio of the intensities of the VI -R telomere band to the control ARO1 chromosomal band as described in the “ Materials and methods .” The values shown are averages of multiple determinations. The input dilution is the input DNA that was used to determine the linear range of PCR amplification (see “ Materials and methods ”). d Comparison of cell doubling times and Mec1-HAp telomere enrichments. Mec1-HAp association increases at PD 70 just as survivors are beginning to arise in the culture, as indicated by the simultaneous decrease in doubling time and appearance of rearranged telomeres (panel a )

    Article Snippet: Telomere length analysis For analysis of the VI- R telomere length, ~10 μg of yeast genomic DNA was digested with 20 units of EcoR V (New England Biolabs) and analyzed by Southern blotting using a 32 P-labeled VI -R PCR product made from primers VI -R70S + VI -R70AS (Table S ) as probe.

    Techniques: Generated, Plasmid Preparation, Cell Culture, Southern Blot, Polymerase Chain Reaction, Isolation, Amplification, Chromatin Immunoprecipitation

    Telomere length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.

    Journal: Genetics

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools

    doi: 10.1534/genetics.112.149120

    Figure Lengend Snippet: Telomere length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A , rnr1-R293A , and rnr1-Y285A ). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1 / RNR1 tlc1Δ / TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.

    Article Snippet: Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen).

    Techniques: Polymerase Chain Reaction, Generated

    Telomerase nucleotide addition processivity is affected by dGTP levels. (A) Schematic illustrating three possible alignments for a telomere ending in -TGGTG with the template region of TLC1. Following reverse transcription and extension of the telomere (with added nucleotides shown in orange), the number of TG dinucleotides between the TGG motif and the following TGGG motif will vary. (B and C) For strains of the indicated genotypes, telomere VI-R was amplified by PCR, cloned, and sequenced. (B) The frequency of having 0, 1, 2, or 3 and higher TG dinucleotides between a TGG and the following TGGG was plotted for each strain. (C) The frequency of having 0, 1, or 2 and higher TG dinucleotides between a TGGG and the following TGGG was plotted for each strain.

    Journal: Genetics

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools

    doi: 10.1534/genetics.112.149120

    Figure Lengend Snippet: Telomerase nucleotide addition processivity is affected by dGTP levels. (A) Schematic illustrating three possible alignments for a telomere ending in -TGGTG with the template region of TLC1. Following reverse transcription and extension of the telomere (with added nucleotides shown in orange), the number of TG dinucleotides between the TGG motif and the following TGGG motif will vary. (B and C) For strains of the indicated genotypes, telomere VI-R was amplified by PCR, cloned, and sequenced. (B) The frequency of having 0, 1, 2, or 3 and higher TG dinucleotides between a TGG and the following TGGG was plotted for each strain. (C) The frequency of having 0, 1, or 2 and higher TG dinucleotides between a TGGG and the following TGGG was plotted for each strain.

    Article Snippet: Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen).

    Techniques: Amplification, Polymerase Chain Reaction, Clone Assay

    rnr1Δ mutants have shortened telomeres due to reduced dGTP levels. (A) An rnr1Δ mutant from the yeast gene deletion collection was backcrossed to a wild-type strain (BY4741) twice. The resulting wild-type and rnr1Δ progeny strains, along with a heterozygous rnr1Δ / RNR1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is plotted for at least four independent isolates (two for the rnr1Δ / RNR1 diploid). (B) Tetrad analysis reveals that an rnr1Δ strain exhibits slow growth. Each column of four colonies is a single tetrad derived from the sporulation of an rnr1Δ / RNR1 diploid followed by the separation of the four haploid spores by micromanipulation. (C) Flow cytometry histograms for the indicated strains derived from B. (D) Wild-type, rnr1Δ , and rnr3Δ strains were assayed for Rnr3, Rnr2, Rnr4, and Sml1 protein levels by protein blot analysis. Tubulin levels were also assayed as a loading control. (E) dNTP pools in the wild-type and rnr1Δ strains were measured. Data are represented as mean ± SE. dGTP levels, as a percentage of total dNTPs, are indicated for each strain.

    Journal: Genetics

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools

    doi: 10.1534/genetics.112.149120

    Figure Lengend Snippet: rnr1Δ mutants have shortened telomeres due to reduced dGTP levels. (A) An rnr1Δ mutant from the yeast gene deletion collection was backcrossed to a wild-type strain (BY4741) twice. The resulting wild-type and rnr1Δ progeny strains, along with a heterozygous rnr1Δ / RNR1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is plotted for at least four independent isolates (two for the rnr1Δ / RNR1 diploid). (B) Tetrad analysis reveals that an rnr1Δ strain exhibits slow growth. Each column of four colonies is a single tetrad derived from the sporulation of an rnr1Δ / RNR1 diploid followed by the separation of the four haploid spores by micromanipulation. (C) Flow cytometry histograms for the indicated strains derived from B. (D) Wild-type, rnr1Δ , and rnr3Δ strains were assayed for Rnr3, Rnr2, Rnr4, and Sml1 protein levels by protein blot analysis. Tubulin levels were also assayed as a loading control. (E) dNTP pools in the wild-type and rnr1Δ strains were measured. Data are represented as mean ± SE. dGTP levels, as a percentage of total dNTPs, are indicated for each strain.

    Article Snippet: Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Derivative Assay, Micromanipulation, Flow Cytometry, Cytometry

    Decreasing dNTP levels results in shorter telomeres. (A) Strains of the indicated genotypes, generated from the sporulation of a dun1Δ / DUN1 sml1Δ / SML1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for four independent isolates of each strain . Similar results were obtained by assaying telomere length by telomere I-L PCR ( i.e. ). (B) Strains in A were assayed for dNTP levels. Four independent isogenic strains for each genotype were analyzed. Data are represented as mean ± SE. (C) Strains of the indicated genotypes were assayed for dNTP levels, as in B.

    Journal: Genetics

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools

    doi: 10.1534/genetics.112.149120

    Figure Lengend Snippet: Decreasing dNTP levels results in shorter telomeres. (A) Strains of the indicated genotypes, generated from the sporulation of a dun1Δ / DUN1 sml1Δ / SML1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for four independent isolates of each strain . Similar results were obtained by assaying telomere length by telomere I-L PCR ( i.e. ). (B) Strains in A were assayed for dNTP levels. Four independent isogenic strains for each genotype were analyzed. Data are represented as mean ± SE. (C) Strains of the indicated genotypes were assayed for dNTP levels, as in B.

    Article Snippet: Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen).

    Techniques: Generated, Polymerase Chain Reaction

    Mec1 and Dun1 function in the same pathway to regulate dNTP pools and telomere length homeostasis. Strains of the indicated genotypes, generated from the sporulation of a dun1Δ / DUN1 tel1Δ / TEL1 mec1-21 / MEC1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼50 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates . Note that the triple mutant is not viable (data not shown).

    Journal: Genetics

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools

    doi: 10.1534/genetics.112.149120

    Figure Lengend Snippet: Mec1 and Dun1 function in the same pathway to regulate dNTP pools and telomere length homeostasis. Strains of the indicated genotypes, generated from the sporulation of a dun1Δ / DUN1 tel1Δ / TEL1 mec1-21 / MEC1 diploid, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼50 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates . Note that the triple mutant is not viable (data not shown).

    Article Snippet: Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen).

    Techniques: Generated, Polymerase Chain Reaction, Mutagenesis

    Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A telomere genomic blot was performed on genomic DNA from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.

    Journal: PLoS ONE

    Article Title: Recombination-Mediated Telomere Maintenance in Saccharomyces cerevisiae Is Not Dependent on the Shu Complex

    doi: 10.1371/journal.pone.0151314

    Figure Lengend Snippet: Rapid senescence and type II survivor formation defect of est2Δ sgs1Δ cells are not rescued by deletion of SHU1 . ( A ) Strains for the indicated genotypes, generated from the sporulation of an est2Δ/EST2 sgs1Δ/SGS1 shu1Δ/SHU1 (YPM5) diploid, were subjected to a liquid culture senescence assay. ( B ) A telomere genomic blot was performed on genomic DNA from strains of the indicated genotypes. The est2Δ , est2Δ shu1Δ , est2Δ sgs1Δ shu1Δ , est2Δ sgs1Δ strains were first passaged for 8 days in a liquid culture senescence assay to generate survivors. A haploid wild-type strain is included (on both sides of the blot), along with the YPM5 diploid.

    Article Snippet: Telomere PCR and telomere length measurements Yeast genomic DNA was isolated using a Wizard Genomic DNA Purification Kit (Promega).

    Techniques: Generated