vector pgem t Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    PerkinElmer pgem t plasmid vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Plasmid Vector, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t plasmid vector/product/PerkinElmer
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pgem t plasmid vector - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    86
    Promega plasmid vector pgem t
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Plasmid Vector Pgem T, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 2429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid vector pgem t/product/Promega
    Average 86 stars, based on 2429 article reviews
    Price from $9.99 to $1999.99
    plasmid vector pgem t - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    99
    Promega pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 21833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/Promega
    Average 99 stars, based on 21833 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    91
    tiangen biotech co pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/tiangen biotech co
    Average 91 stars, based on 128 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    85
    Thermo Fisher vector pgem t
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Vector Pgem T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pgem t/product/Thermo Fisher
    Average 85 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    vector pgem t - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    92
    TaKaRa pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/TaKaRa
    Average 92 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    88
    GenScript pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/GenScript
    Average 88 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    88
    Stratagene pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/Stratagene
    Average 88 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    88
    Boehringer Mannheim pgem t vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/Boehringer Mannheim
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    99
    Promega pgem t easy plasmid vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Plasmid Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 8316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy plasmid vector/product/Promega
    Average 99 stars, based on 8316 article reviews
    Price from $9.99 to $1999.99
    pgem t easy plasmid vector - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    78
    Stratagene plasmids pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Plasmids Pgem T Easy Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids pgem t easy vector/product/Stratagene
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plasmids pgem t easy vector - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    92
    Thermo Fisher pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Thermo Fisher
    Average 92 stars, based on 933 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    79
    Promega transcription vector pgem t
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Transcription Vector Pgem T, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription vector pgem t/product/Promega
    Average 79 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    transcription vector pgem t - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    88
    Stratagene pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Stratagene
    Average 88 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    91
    TaKaRa pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/TaKaRa
    Average 91 stars, based on 323 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    88
    Sangon Biotech pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 88/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Sangon Biotech
    Average 88 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    90
    Millipore pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Millipore
    Average 90 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    99
    Promega pgem t vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector system/product/Promega
    Average 99 stars, based on 2729 article reviews
    Price from $9.99 to $1999.99
    pgem t vector system - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    88
    Bio-Rad pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Bio-Rad
    Average 88 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    78
    Promega pgem t pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t pgem t easy vector/product/Promega
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    pgem t pgem t easy vector - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    86
    tiangen biotech co pgem t vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector System, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector system/product/tiangen biotech co
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pgem t vector system - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    92
    tiangen biotech co pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/tiangen biotech co
    Average 92 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    85
    Fisher Scientific pgem t vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector System, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector system/product/Fisher Scientific
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pgem t vector system - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    91
    Thermo Fisher pgem t easy vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector system/product/Thermo Fisher
    Average 91 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector system - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    85
    GenScript pgem t vector p part
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vector P Part, supplied by GenScript, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector p part/product/GenScript
    Average 85 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    pgem t vector p part - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    87
    TaKaRa pgem t easy vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector system/product/TaKaRa
    Average 87 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector system - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

    87
    tiangen biotech co pgem t easy vector system
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Easy Vector System, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector system/product/tiangen biotech co
    Average 87 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector system - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

    81
    Promega escherichia coli plasmid vector pgem t easy
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Escherichia Coli Plasmid Vector Pgem T Easy, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli plasmid vector pgem t easy/product/Promega
    Average 81 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    escherichia coli plasmid vector pgem t easy - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    99
    Promega pgem t vectors
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vectors/product/Promega
    Average 99 stars, based on 2039 article reviews
    Price from $9.99 to $1999.99
    pgem t vectors - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher pgem t vectors
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vectors/product/Thermo Fisher
    Average 89 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    pgem t vectors - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    85
    Promega pmd 19t pgem t easy vector
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pmd 19t Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd 19t pgem t easy vector/product/Promega
    Average 85 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pmd 19t pgem t easy vector - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    88
    tiangen biotech co pgem t vectors
    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into <t>pGEM-T</t> plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).
    Pgem T Vectors, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vectors/product/tiangen biotech co
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    pgem t vectors - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    Image Search Results


    Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into pGEM-T plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Beneficial Effects of Myo-Inositol Oxygenase Deficiency in Cisplatin-Induced AKI

    doi: 10.1681/ASN.2016070744

    Figure Lengend Snippet: Cisplatin treatment per se upregulates MIOX via demethylation of its promoter. WT mice treated with cisplatin had an increased renal expression of MIOX, and it was confined to the tubules (A–C). No significant MIOX expression was seen in glomeruli. Genomic DNA was isolated from kidneys of WT and cisplatin-treated mice and subjected to bisulfite treatment. Promoter fragments were isolated by PCR, cloned into pGEM-T plasmid, and transformed in DH5 α cells. Ten clones from each variable were selected and subjected to nucleotide sequencing to assess the efficiency of conversion (cytosine to thymine) of CpG dinucleotides. Nucleotide analyses revealed eight CpG dinucleotides at −19, −22, −42, −48, −73, −424, −436 and −456 bp sites. In WT, untreated control mice samples, CpG at −22 and −436 bp were found to be unmethylated, whereas six others were methylated (D and E). The samples from cisplatin-treated mice were found to have all of the CpG dinucleotides unmethylated, except for −424 and −456. Overall, the efficiency of conversion of C to T nucleotide was > 95%. The percentages of methylation of individual dinucleotide residues are presented as bar graphs, which indicate that cisplatin induces hypomethylation that is likely responsible for the MIOX upregulation (F).

    Article Snippet: Sigma-Aldrich: cisplatin (# P4394), anti– β -actin antibody (# A5441), Caspase-3 assay kit, Colorimetric (# CASP-3C), poly-(deoxyinosinic-deoxycytidylic) acid sodium salt (# P4929), NAC (# A7250); BioAssay Systems: QuantiChrom creatinine assay kit (# DICT-500) and QuantiChrom urea assay kit (# DIUR-500); Life Technologies: Power SYBR Green PCR Master Mix (# 4367659), Chloromethyl derivative of 2′, 7′-dichlorofluorescein diacetate (CM-H2 DCF-DA, # C6827), 4′-6-diamidino-2-phenylindole (DAPI, # D1306), and TRIzol Reagent (# 15596026); Cell Signaling Technology: anti–cleaved caspase-3 antibody (# D175), anti-p53 (1C12) antibody (# 2524), and anti-p65 antibody (# D14E12); Abcam: anti–lamin B1(ab16048); Enzo Life Sciences: DNA methylation gold kit (# D5005); Santa Cruz Biotechnology: anti-Bax antibody (# SC-526), anti–NOX-4 antibody (# SC-21860), anti-p53 antibody (# SC-6243); Roche Diagnostic: In Situ Cell Death Detection Kit, Fluorescein (# 11684795910); Cayman Chemicals: GSH assay kit (# 703002); Promega Corporation: NF- κ B EMSA oligo (# E329A) and pGEM-T plasmid vector; Perkin Elmer: ATP γ -32p (# BLU002A); and Thermo Scientific: polynucleotide kinase (EK0032).

    Techniques: Mouse Assay, Expressing, Isolation, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Transformation Assay, Sequencing, Methylation