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  • 99
    Promega vector pgem t
    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector <t>pGEM-T</t> in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).
    Vector Pgem T, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 6178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega pgem t vector system i
    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector <t>pGEM-T</t> in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).
    Pgem T Vector System I, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene pgem t vector
    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector <t>pGEM-T</t> in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).
    Pgem T Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa pgem t vector
    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector <t>pGEM-T</t> in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).
    Pgem T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher pgem t vector
    The BMPR-IA gene coding sequence and <t>pGEM-T/BMPR-IA</t> (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed <t>ds-oligos</t> of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020 b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA
    Pgem T Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    tiangen biotech co pgem t vector
    The BMPR-IA gene coding sequence and <t>pGEM-T/BMPR-IA</t> (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed <t>ds-oligos</t> of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020 b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA
    Pgem T Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/tiangen biotech co
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    99
    Promega vector pgem t easy
    Transformation frequencies of H. pylori mutants. (A) H. pylori strain 26695 and derivative strains were transformed by homologous recombination with p801R (an 801-bp H. pylori rpsL fragment from a streptomycin-resistant [Str r ] strain cloned into <t>pGEM-T</t>
    Vector Pgem T Easy, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector pGEM-T in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).

    Journal: Infection and Immunity

    Article Title: Molecular Characterization of Mycoplasma arthritidis Variable Surface Protein MAA2

    doi:

    Figure Lengend Snippet: Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector pGEM-T in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).

    Article Snippet: For sequencing through the repeat regions of maa2 , sequences amplified by PCR from forward and reverse primers NT and HMPR were cloned into vector pGEM-T as described above, and a series of nested deletions was prepared by using the Erase-A-Base system (Promega).

    Techniques: Expressing, Recombinant, Plasmid Preparation, SDS Page, Staining, Clone Assay, Variant Assay

    Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: pGEM-T/EcoRI/XhoI; 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.

    Journal:

    Article Title: Cloning and molecular characterization of Δ12-fatty acid desaturase gene from Mortierella isabellina

    doi: 10.3748/wjg.v12.i21.3373

    Figure Lengend Snippet: Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: pGEM-T/EcoRI/XhoI; 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.

    Article Snippet: PCR product about 1.2 kb long was gel-purified by electrophoresis and cloned into pGEM-T vector.

    Techniques: Recombinant, Plasmid Preparation, Marker, Polymerase Chain Reaction

    Trans -acting factors specifically binding to the editing sites in the extracts of tobacco chloroplasts. ( A ) UV-crosslinking was performed with a respective RNA probe that was labeled with 32 P at +1 (C to be edited). Lanes 1, without competitor RNA; lanes 2, a 100-fold molar excess of unlabeled probe RNA was added as a competitor; lanes 3, a 100-fold molar excess of control RNA that was a 161 nt transcript of a pGEM-T vector was added as a competitor. Free indicates the bands of a free probe that migrated in front of the protein bands on SDS–PAGE. ( B ) Comparison of the electrophoretic mobilities of p95s binding to ndhB-9 (lane 1) and ndhF-1 (lane 2).

    Journal: Nucleic Acids Research

    Article Title: Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts

    doi: 10.1093/nar/gkm1026

    Figure Lengend Snippet: Trans -acting factors specifically binding to the editing sites in the extracts of tobacco chloroplasts. ( A ) UV-crosslinking was performed with a respective RNA probe that was labeled with 32 P at +1 (C to be edited). Lanes 1, without competitor RNA; lanes 2, a 100-fold molar excess of unlabeled probe RNA was added as a competitor; lanes 3, a 100-fold molar excess of control RNA that was a 161 nt transcript of a pGEM-T vector was added as a competitor. Free indicates the bands of a free probe that migrated in front of the protein bands on SDS–PAGE. ( B ) Comparison of the electrophoretic mobilities of p95s binding to ndhB-9 (lane 1) and ndhF-1 (lane 2).

    Article Snippet: The amplified fragments were cloned into a pGEM-T vector using the pGEM-T Vector System (Promega).

    Techniques: Binding Assay, Labeling, Plasmid Preparation, SDS Page

    Disruption of BBA74 by insertion of kanamycin resistance cassette. (A) Schematic diagram of mutant construct. The region of lp54 between BBA73 and BBA76 was amplified by PCR using primers p73F/p76R and cloned into pGEM-T. The insertion of the kanamycin

    Journal:

    Article Title: Comparative Transcriptional Profiling of Borrelia burgdorferi Clinical Isolates Differing in Capacities for Hematogenous Dissemination

    doi: 10.1128/IAI.73.10.6791-6802.2005

    Figure Lengend Snippet: Disruption of BBA74 by insertion of kanamycin resistance cassette. (A) Schematic diagram of mutant construct. The region of lp54 between BBA73 and BBA76 was amplified by PCR using primers p73F/p76R and cloned into pGEM-T. The insertion of the kanamycin

    Article Snippet: A 2,327-bp DNA fragment spanning BBA73 to BBA76 (nucleotides 50601 to 52908 on plasmid lp54; GenBank accession number, ) was amplified by PCR using primers pA73F and pA76R (Table ) as forward and reverse primers, respectively, and cloned into the plasmid vector pGEM-T (Promega).

    Techniques: Mutagenesis, Construct, Amplification, Polymerase Chain Reaction, Clone Assay

    (A) Southern blotting of EcoRI-digested total DNA from FD-C-infected and FD-D-infected and healthy (H) periwinkles probed with a DIG-labeled malG gene amplicon obtained through PCR driven by primer pair malG _F/ malG _R (C+, probe positive control represented by pGEM-T- malG1 plasmid). (B) Electrophoresis separation of amplicons obtained following PCR of total DNA from FD-C-infected and FD-D-infected periwinkles with copy-specific primer pairs (002 and 005), according to the draft genome of FD92, and from healthy periwinkle. *, nonspecific PCR product.

    Journal: Applied and Environmental Microbiology

    Article Title: Genetic Diversity of Flavescence Dorée Phytoplasmas at the Vineyard Scale

    doi: 10.1128/AEM.03123-18

    Figure Lengend Snippet: (A) Southern blotting of EcoRI-digested total DNA from FD-C-infected and FD-D-infected and healthy (H) periwinkles probed with a DIG-labeled malG gene amplicon obtained through PCR driven by primer pair malG _F/ malG _R (C+, probe positive control represented by pGEM-T- malG1 plasmid). (B) Electrophoresis separation of amplicons obtained following PCR of total DNA from FD-C-infected and FD-D-infected periwinkles with copy-specific primer pairs (002 and 005), according to the draft genome of FD92, and from healthy periwinkle. *, nonspecific PCR product.

    Article Snippet: In the case of mixed infections (presence of double peaks in the analyzed pherograms from the sequencing of the original PCR amplicon), purified PCR products were ligated into pGEM-T easy cloning vector following the manufacturer's instructions (pGEM-T clone kit; Promega, Madison, WI) and transformed into Escherichia coli DH5α competent cells by heat shock.

    Techniques: Southern Blot, Infection, Labeling, Amplification, Polymerase Chain Reaction, Positive Control, Plasmid Preparation, Electrophoresis

    The BMPR-IA gene coding sequence and pGEM-T/BMPR-IA (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed ds-oligos of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020 b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA

    Journal: BMC Musculoskeletal Disorders

    Article Title: Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathway

    doi: 10.1186/s12891-018-1966-1

    Figure Lengend Snippet: The BMPR-IA gene coding sequence and pGEM-T/BMPR-IA (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed ds-oligos of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020 b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA

    Article Snippet: The ds-oligos were inserted into the pGEM-T vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase.

    Techniques: IA, Sequencing, Plasmid Preparation, Agarose Gel Electrophoresis, Molecular Weight, Marker, Polymerase Chain Reaction

    Transformation frequencies of H. pylori mutants. (A) H. pylori strain 26695 and derivative strains were transformed by homologous recombination with p801R (an 801-bp H. pylori rpsL fragment from a streptomycin-resistant [Str r ] strain cloned into pGEM-T

    Journal: Journal of Bacteriology

    Article Title: DprB Facilitates Inter- and Intragenomic Recombination in Helicobacter pylori

    doi: 10.1128/JB.00346-12

    Figure Lengend Snippet: Transformation frequencies of H. pylori mutants. (A) H. pylori strain 26695 and derivative strains were transformed by homologous recombination with p801R (an 801-bp H. pylori rpsL fragment from a streptomycin-resistant [Str r ] strain cloned into pGEM-T

    Article Snippet: An 801-bp DNA sequence encompassing H. pylori rpsL from Strr H. pylori strain 26695 ( , ) was obtained by PCR using primers rpsL801-F and rpsL801-R (see Table S2 in the supplemental material) and cloned into the vector pGEM-T Easy (Promega, Madison, WI) to create plasmid p801R (see Table S1), which was used as donor DNA for homologous transformation.

    Techniques: Transformation Assay, Homologous Recombination, Clone Assay

    Cross-species complementation. (A) Complementation of the E. coli ruvC mutant by the cloned H. pylori dprB and ruvC genes. E. coli cells carrying pGEM-T Easy-derived plasmids on an LB plate with ampicillin were subjected to a range of UV exposures, and

    Journal: Journal of Bacteriology

    Article Title: DprB Facilitates Inter- and Intragenomic Recombination in Helicobacter pylori

    doi: 10.1128/JB.00346-12

    Figure Lengend Snippet: Cross-species complementation. (A) Complementation of the E. coli ruvC mutant by the cloned H. pylori dprB and ruvC genes. E. coli cells carrying pGEM-T Easy-derived plasmids on an LB plate with ampicillin were subjected to a range of UV exposures, and

    Article Snippet: An 801-bp DNA sequence encompassing H. pylori rpsL from Strr H. pylori strain 26695 ( , ) was obtained by PCR using primers rpsL801-F and rpsL801-R (see Table S2 in the supplemental material) and cloned into the vector pGEM-T Easy (Promega, Madison, WI) to create plasmid p801R (see Table S1), which was used as donor DNA for homologous transformation.

    Techniques: Mutagenesis, Clone Assay, Derivative Assay