vectashield mounting medium Search Results


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  • 99
    Vector Laboratories vectrashield mounting medium
    The colocalization of mCherryTM and cis/medial Golgi marker GOLM1. COS-1 cells were transfected with pSARMXmCherryTM WT or mutant variant. 48 h later, they were fixed with 4% formaldehyde and permeabilized with Tween 20. All samples were immunostained with primary rabbit anti-GOLM1 antibody and then with secondary antibody against rabbit IgG conjugated with Alexa Fluor TM Plus 488 and mounted into <t>Vectashield</t> mounting medium with DAPI. Samples were imaged with spinning disk confocal microscope (Andor). Magnification 600×; scale bar 20 µm.
    Vectrashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectrashield mounting medium/product/Vector Laboratories
    Average 99 stars, based on 284 article reviews
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    vectrashield mounting medium - by Bioz Stars, 2020-08
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    99
    Millipore vectashield mounting medium
    The colocalization of mCherryTM and cis/medial Golgi marker GOLM1. COS-1 cells were transfected with pSARMXmCherryTM WT or mutant variant. 48 h later, they were fixed with 4% formaldehyde and permeabilized with Tween 20. All samples were immunostained with primary rabbit anti-GOLM1 antibody and then with secondary antibody against rabbit IgG conjugated with Alexa Fluor TM Plus 488 and mounted into <t>Vectashield</t> mounting medium with DAPI. Samples were imaged with spinning disk confocal microscope (Andor). Magnification 600×; scale bar 20 µm.
    Vectashield Mounting Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Millipore
    Average 99 stars, based on 148 article reviews
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    94
    Bio-Rad vectashield mounting medium
    The colocalization of mCherryTM and cis/medial Golgi marker GOLM1. COS-1 cells were transfected with pSARMXmCherryTM WT or mutant variant. 48 h later, they were fixed with 4% formaldehyde and permeabilized with Tween 20. All samples were immunostained with primary rabbit anti-GOLM1 antibody and then with secondary antibody against rabbit IgG conjugated with Alexa Fluor TM Plus 488 and mounted into <t>Vectashield</t> mounting medium with DAPI. Samples were imaged with spinning disk confocal microscope (Andor). Magnification 600×; scale bar 20 µm.
    Vectashield Mounting Medium, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Bio-Rad
    Average 94 stars, based on 47 article reviews
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    vectashield mounting medium - by Bioz Stars, 2020-08
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    92
    Thermo Fisher vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Thermo Fisher
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    91
    Cell Signaling Technology Inc vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Cell Signaling Technology Inc
    Average 91 stars, based on 10 article reviews
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    91
    Eurobio vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Eurobio, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Eurobio
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    92
    Reactolab vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Reactolab, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Fisher Scientific
    Average 92 stars, based on 63 article reviews
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    90
    Clinisciences vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Clinisciences, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Avantor
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    92
    Agilent technologies vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cedarlane vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Cedarlane
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    92
    Vector Biolabs vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Vector Biolabs
    Average 92 stars, based on 23 article reviews
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    vectashield mounting medium - by Bioz Stars, 2020-08
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    92
    Biozol Diagnostica Vertrieb GmbH vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield mounting medium/product/Biozol Diagnostica Vertrieb GmbH
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    90
    Applygen Technologies vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vectashield mounting medium - by Bioz Stars, 2020-08
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    92
    Axxora vectashield mounting medium
    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with <t>Vectashield</t> Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.
    Vectashield Mounting Medium, supplied by Axxora, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The colocalization of mCherryTM and cis/medial Golgi marker GOLM1. COS-1 cells were transfected with pSARMXmCherryTM WT or mutant variant. 48 h later, they were fixed with 4% formaldehyde and permeabilized with Tween 20. All samples were immunostained with primary rabbit anti-GOLM1 antibody and then with secondary antibody against rabbit IgG conjugated with Alexa Fluor TM Plus 488 and mounted into Vectashield mounting medium with DAPI. Samples were imaged with spinning disk confocal microscope (Andor). Magnification 600×; scale bar 20 µm.

    Journal: Viruses

    Article Title: Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

    doi: 10.3390/v10100575

    Figure Lengend Snippet: The colocalization of mCherryTM and cis/medial Golgi marker GOLM1. COS-1 cells were transfected with pSARMXmCherryTM WT or mutant variant. 48 h later, they were fixed with 4% formaldehyde and permeabilized with Tween 20. All samples were immunostained with primary rabbit anti-GOLM1 antibody and then with secondary antibody against rabbit IgG conjugated with Alexa Fluor TM Plus 488 and mounted into Vectashield mounting medium with DAPI. Samples were imaged with spinning disk confocal microscope (Andor). Magnification 600×; scale bar 20 µm.

    Article Snippet: The coverslips were mounted in Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Marker, Transfection, Mutagenesis, Variant Assay, Microscopy

    Plasma membrane distribution of M-PMV Env. The COS-1 cells were either transfected with plasmids encoding M-PMV genomic DNA with: WT Env (pSARMX-WT; panel A ), I18A Env (pSARMX-I18A; panel B ), or Y22A Env (pSARMX-Y22A; panel C ) or an M-PMV Env expression vector (pTMT-WT; panel D ). 48 h post-transfection cells were incubated with goat anti-M-PMV antibody on ice for 25 min to bind surface exposed M-PMV Env. Formaldehyde (4%) fixed cells were immunostained for CA protein with rabbit anti-CA antibody (except for the cells transfected with pTMT-WT,). Env was visualized using secondary anti-goat IgG antibody conjugated with Alexa Fluor ® 350. Samples were mounted in Vectashield mounting media and imaged on an Olympus cell^R microscope. The original blue color of the AF350 signal was changed to cyan for increased contrast. Magnification 600×; scale bars 20 µm.

    Journal: Viruses

    Article Title: Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

    doi: 10.3390/v10100575

    Figure Lengend Snippet: Plasma membrane distribution of M-PMV Env. The COS-1 cells were either transfected with plasmids encoding M-PMV genomic DNA with: WT Env (pSARMX-WT; panel A ), I18A Env (pSARMX-I18A; panel B ), or Y22A Env (pSARMX-Y22A; panel C ) or an M-PMV Env expression vector (pTMT-WT; panel D ). 48 h post-transfection cells were incubated with goat anti-M-PMV antibody on ice for 25 min to bind surface exposed M-PMV Env. Formaldehyde (4%) fixed cells were immunostained for CA protein with rabbit anti-CA antibody (except for the cells transfected with pTMT-WT,). Env was visualized using secondary anti-goat IgG antibody conjugated with Alexa Fluor ® 350. Samples were mounted in Vectashield mounting media and imaged on an Olympus cell^R microscope. The original blue color of the AF350 signal was changed to cyan for increased contrast. Magnification 600×; scale bars 20 µm.

    Article Snippet: The coverslips were mounted in Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Transfection, Expressing, Plasmid Preparation, Incubation, Microscopy

    Rab-markers based identification of intracellular vesicles carrying mCherryTM protein variants. The COS-1 cells co-transfected with a combination of pSARMXmCherryTM and enhanced green fluorescent protein (EGFP)-tagged endosomal marker coding plasmids were fixed with 4% formaldehyde 24 h post-transfection. Upon mounting in Vectashield media the samples were imaged using an Olympus cell^R microscope. White arrowheads indicate colocalization of the mCherryTM signal and the corresponding marker. Magnification 600×; scale bars 20 µm.

    Journal: Viruses

    Article Title: Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

    doi: 10.3390/v10100575

    Figure Lengend Snippet: Rab-markers based identification of intracellular vesicles carrying mCherryTM protein variants. The COS-1 cells co-transfected with a combination of pSARMXmCherryTM and enhanced green fluorescent protein (EGFP)-tagged endosomal marker coding plasmids were fixed with 4% formaldehyde 24 h post-transfection. Upon mounting in Vectashield media the samples were imaged using an Olympus cell^R microscope. White arrowheads indicate colocalization of the mCherryTM signal and the corresponding marker. Magnification 600×; scale bars 20 µm.

    Article Snippet: The coverslips were mounted in Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Transfection, Marker, Microscopy

    Nuclear translocation of AhR. LS180 cells were seeded on chamber slides and cultured for 2 days. Then, the cells were incubated for 90 minutes with DMSO (0.1% v/v), TCDD (5 nM), 4-Me-indole (1, 10, and 100 μ M), and 7-MeO-indole (1, 10, and 100 μ M). Microscopic specimens were prepared according to the common protocol, using Alexa Fluor 488 labeled primary antibody against AhR, 4′,6-diamino-2-phenylindole, and VectaShield Antifade Mounting Medium. AhR was visualized and evaluated using a fluorescence microscope. Percentage of cells with nuclear AhR was calculated by visual comparison of antibody signal intensity in the nucleus and cytosol, when at least four random locations per sample with approximately 100 cells were evaluated. Experiments were performed in two consecutive cell passages, with all tested compounds in duplicate. The representative images are shown together with an inset containing the total and AhR-positive counts of cells.

    Journal: Molecular Pharmacology

    Article Title: Methylindoles and Methoxyindoles are Agonists and Antagonists of Human Aryl Hydrocarbon Receptor

    doi: 10.1124/mol.118.112151

    Figure Lengend Snippet: Nuclear translocation of AhR. LS180 cells were seeded on chamber slides and cultured for 2 days. Then, the cells were incubated for 90 minutes with DMSO (0.1% v/v), TCDD (5 nM), 4-Me-indole (1, 10, and 100 μ M), and 7-MeO-indole (1, 10, and 100 μ M). Microscopic specimens were prepared according to the common protocol, using Alexa Fluor 488 labeled primary antibody against AhR, 4′,6-diamino-2-phenylindole, and VectaShield Antifade Mounting Medium. AhR was visualized and evaluated using a fluorescence microscope. Percentage of cells with nuclear AhR was calculated by visual comparison of antibody signal intensity in the nucleus and cytosol, when at least four random locations per sample with approximately 100 cells were evaluated. Experiments were performed in two consecutive cell passages, with all tested compounds in duplicate. The representative images are shown together with an inset containing the total and AhR-positive counts of cells.

    Article Snippet: The next day, nuclei were stained with 4′,6-diamino-2-phenylindole, and cells were enclosed by VectaShield Antifade Mounting Medium (Vector Laboratories Inc., Peterborough, UK).

    Techniques: Translocation Assay, Cell Culture, Incubation, Labeling, Fluorescence, Microscopy

    Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with Vectashield Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.

    Journal: PLoS ONE

    Article Title: Comparative Proteomics Reveals Novel Components at the Plasma Membrane of Differentiated HepaRG Cells and Different Distribution in Hepatocyte- and Biliary-Like Cells

    doi: 10.1371/journal.pone.0071859

    Figure Lengend Snippet: Analysis of differentially expressed proteins in hepatocyte- and biliary-like HepaRG cells (I). Cells were seeded on coverslips in 6-well plates and either differentiated (D) or maintained untreated, as control (ND). Expression of Annexin A1 (panel a) and Cyclophilin A (panel b) in hepatic (H) and biliary (B) cells was evidenced using specific Abs. Albumin expression was also investigated as a marker for differentiation to adult hepatocytes (panels a and b). Samples were analyzed with a Zeiss LSM 710 confocal microscope following mounting with Vectashield Mounting Medium containing DAPI, to visualize the nuclei (blue). Images were taken with the 63x objective and processed with ZEN software. Scale bar is 20 µm.

    Article Snippet: The samples were mounted with Vectashield Mounting Medium (Invitrogen) containing DAPI (4, 6-diamidino-2-phenylindole) to visualize the nuclei.

    Techniques: Expressing, Marker, Microscopy, Software