Journal: Scientific Reports
Article Title: Vasohibin-1 rescues erectile function through up-regulation of angiogenic factors in the diabetic mice
Figure Lengend Snippet: VASH1 protein transfer increases the cavernous expression of angiogenic factors in diabetic mice. ( a , b ) Angiopoietin-1 (Ang1, green) and PECAM1 (red), or vascular endothelial growth factor (VEGF, green) and PECAM-1 (red) staining in cavernous tissue from age-matched control and streptozotocin-induced diabetic mice 2 weeks after receiving repeated intracavernous injections of PBS (days − 3 and 0) or VASH1 protein (days − 3 and 0; 4 μg/20 μL). Scale bar = 100 µm. ( c , d ) Ang1 and VEGF-immunopositive areas were quantified by Image J. Each bar depicts the mean (± SE) values from N = 6 animals per group. * P
Article Snippet: Frozen tissue sections (20-μm thick) were incubated with antibodies to VASH1 (Santa Cruz Biotechnology Inc., Dallas, TX USA; 1:50), FITC-conjugated isolectin B4 (IB4, Sigma-Aldrich, MO, USA; 1:50), PECAM-1 (Millipore, Temecula, CA, USA; 1:50), NG2 (Millipore; 1:50), phospho-eNOS (Ser1177, Cell Signaling, MA, USA; 1:50), occludin (NOVUS Biologicals, Centennial, CO, USA; 1:50), claudin-1 (Thermo Fisher, Waltham, Massachusetts, U.S. 1:50), Ang1 (Abcam, Cambridge, U.K.; 1:50), or VEGF (Santa Cruz Biotechnology; 1:50) at 4 °C overnight.
Techniques: Expressing, Mouse Assay, Staining