vascular endothelial growth factor vegf a Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Thermo Fisher vascular endothelial growth factor vegf
    (a) EMA immunostain highlighting whorls of meningioma cells (200x); (b) intense and diffuse <t>VEGF</t> expression in an anaplastic meningioma (200x); (c) <t>Ki67</t> immunostain revealing a moderate proliferative activity in a case of atypical meningioma (200x); (d) benign meningioma with significant PR expression (200x); (e) CD20 highlighting rare B lymphocytes (100x); (f) CD3 showing a large population of T lymphocytes (100x).
    Vascular Endothelial Growth Factor Vegf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Thermo Fisher
    Average 95 stars, based on 548 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    96
    Millipore vascular endothelial growth factor vegf
    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; <t>VEGF.</t> ( F ) <t>IFN</t> gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P
    Vascular Endothelial Growth Factor Vegf, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Millipore
    Average 96 stars, based on 513 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    94
    R&D Systems vascular endothelial growth factor vegf
    BMP2 and <t>BMP4</t> stimulate eNOS phosphorylation in PAECs. A and B , representative Western blots of bPAECs stimulated with BMP2 ( A ) or BMP4 ( B ) for various times and analyzed for eNOS phosphorylation at serine 1179. <t>VEGF</t> was used as a positive control. C
    Vascular Endothelial Growth Factor Vegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/R&D Systems
    Average 94 stars, based on 1106 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    89
    Agilent technologies vascular endothelial growth factor a vegf a
    Activin promotes tumorigenicity of breast cancer cells in immunocompromised mice. ( a ) Stable overexpression of activin-A in MCF-7 enhances (i), whereas stable knockdown of activin-A in MDA-MB-231 cells (ii) reduces their tumor-forming ability in nude mice. Shown below are the representative images of the tumors formed s.c. ( b ) Immunohistochemical analysis of MCF-7-overexpressing tumors shows EMT-like changes and higher ki-67 index. ( c ) Treatment of MCF-7 cells with activin-A (i) or overexpression of activin-A in MCF-7 cells (ii) results in increased levels of vascular endothelial growth <t>factor-A</t> <t>(VEGF-A).</t> ( d ) Luciferase reporter assay in HEK 293T cells shows that activin-A regulates VEGF promoter activity. ( e ) Tail vein injection of activin-A-overexpressing MCF-7 cells shows better tumor-forming ability in the livers of nude mice (i). Normal is shown here as the reference from an animal without injection of any cells. The panel below (ii) shows the hematoxylin and eosin (H E) staining of the liver tissue sections. The graph (iii) and (iv) shows number and size of nodules formed in the liver per animal. ( f ) CD44 high/ CD24 low fluorescence-activated cell sorting (FACS) analysis of activin-A-overexpressing or knockdown cells shows that activin-A influences stemness of breast cancer cells (i). Quantitative PCR analysis shows that treatment of MCF-7 or MDA-MB-231 cells with recombinant activin-A induces various markers of stemness (ii).
    Vascular Endothelial Growth Factor A Vegf A, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor a vegf a/product/Agilent technologies
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor a vegf a - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc anti vascular endothelial growth factor vegf a
    <t>VEGF-A</t> and -C protein expression of breast cancer MCF-7 cells in the <t>COX-2-shRNA</t> group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.
    Anti Vascular Endothelial Growth Factor Vegf A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vascular endothelial growth factor vegf a/product/Cell Signaling Technology Inc
    Average 97 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    anti vascular endothelial growth factor vegf a - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    89
    Reliatech vascular endothelial growth factor a vegf a
    <t>VEGF-A</t> and -C protein expression of breast cancer MCF-7 cells in the <t>COX-2-shRNA</t> group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.
    Vascular Endothelial Growth Factor A Vegf A, supplied by Reliatech, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor a vegf a/product/Reliatech
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor a vegf a - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology vascular endothelial growth factor vegf
    Mechanical ventilation increased MMP-7, cyclin D1, and <t>VEGF</t> in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to <t>β-actin.</t> Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p
    Vascular Endothelial Growth Factor Vegf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Santa Cruz Biotechnology
    Average 93 stars, based on 514 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Millipore vegf
    Deletion of TXNIP does not alter <t>VEGFR2/Akt</t> activation under hyperoxia. Wild type (WT) and TXNIP knockout (TKO) mice were subjected to hyperoxia (75% O2, p7–p12). Activation of VEGFR2 ( A ) and Akt ( B ) were examined as downstream signal of <t>VEGF</t> in p12 WT and TKO retinas. TKO showed significant decrease in phosphorylation of VEGFR-2 and Akt compared to WT under normoxic condition. We did not detect significant change in the activation of VEGFR2 and its downstream Akt in retinas from TKO and WT in response to hyperoxia. (*P
    Vegf, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1674 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/Millipore
    Average 92 stars, based on 1674 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Abcam vascular endothelial growth factor vegf
    Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, <t>Bcl‐2‐associated</t> X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.
    Vascular Endothelial Growth Factor Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Abcam
    Average 93 stars, based on 339 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher vascular endothelial growth factor a vegf a mrna
    Kaplan-Meier curves of recurrence for the 2nd cohort of 47 CK20+ pN0 CRC patients after follow-up for 60 months based on <t>VEGF-A</t> <t>mRNA</t> copy numbers
    Vascular Endothelial Growth Factor A Vegf A Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor a vegf a mrna/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor a vegf a mrna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Bender MedSystems vascular endothelial growth factor vegf a
    Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and <t>VEGF-A</t> ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon <t>γ-induced</t> protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.
    Vascular Endothelial Growth Factor Vegf A, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf a/product/Bender MedSystems
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf a - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    97
    Millipore materials vegf a
    Influence of vascular endothelial growth factor <t>(VEGF),</t> axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p
    Materials Vegf A, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials vegf a/product/Millipore
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    materials vegf a - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    92
    Cusabio vascular endothelial growth factor vegf
    Expression of homing and angiogenic signals of the myocardium 7 days after cardiac transfer of green fluorescent protein <t>(GFP)-Luc-mesenchymal</t> stem cell (MSCs). Fluorescent immunohistochemistry of the bioluminescence positive myocardial areas 7 days after intramyocardial [left panel (A,C,E,G) ] or intracoronary [right panel (B,D,F,H) ] GFP-Luc-MSCs delivery shows increased expression of homing signals cadherin (A,B) , and angiogenic factors fibroblast growth factor 2 (FGF2) (C,D) and vascular endothelial growth factor <t>(VEGF)</t> (E,F) in group IM. Infarct area border zone (G,H) exhibited higher number of myocardial cells and higher level of VEGF expression in group IM (G) . Hoechst staining of the nuclei, 40× magnification. Expression of homing signals cadherin (I) , stromal-derived factor-1alpha (J) , and angiogenic factors FGF2 (K) and VEGF (L) .
    Vascular Endothelial Growth Factor Vegf, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Cusabio
    Average 92 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    (a) EMA immunostain highlighting whorls of meningioma cells (200x); (b) intense and diffuse VEGF expression in an anaplastic meningioma (200x); (c) Ki67 immunostain revealing a moderate proliferative activity in a case of atypical meningioma (200x); (d) benign meningioma with significant PR expression (200x); (e) CD20 highlighting rare B lymphocytes (100x); (f) CD3 showing a large population of T lymphocytes (100x).

    Journal: The Scientific World Journal

    Article Title: Biological and Demographic Profile of Meningiomas in a Cohort of Egyptian Patients: Impact on Tumor Recurrence

    doi: 10.1155/2013/375139

    Figure Lengend Snippet: (a) EMA immunostain highlighting whorls of meningioma cells (200x); (b) intense and diffuse VEGF expression in an anaplastic meningioma (200x); (c) Ki67 immunostain revealing a moderate proliferative activity in a case of atypical meningioma (200x); (d) benign meningioma with significant PR expression (200x); (e) CD20 highlighting rare B lymphocytes (100x); (f) CD3 showing a large population of T lymphocytes (100x).

    Article Snippet: The used primary antibodies (at 1 : 100 dilution): vascular endothelial growth factor (VEGF), clone: VG; Ki67, clone: SP6; progesterone receptor (PR), clone: SP2; CD20 Ab-1, clone: L26; and CD3epsilon Ab-2, clone: PS1, as well as the detection kit (UltraVision Detection System Anti-Polyvalent, HRP/DAB, Ready-To-Use), were purchased from Thermo Scientific Lab Vision, USA.

    Techniques: Expressing, Activity Assay

    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

    Journal: Scientific Reports

    Article Title: Differential response of rat strains to obesogenic diets underlines the importance of genetic makeup of an individual towards obesity

    doi: 10.1038/s41598-017-09149-6

    Figure Lengend Snippet: Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

    Article Snippet: Rat Cytokine/Chemokine Profile The adipocytokines such as leptin, macrophage inflammatory protein-1 alpha (MIP-1α, CCL3), interleukin-4 (IL-4), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), macrophage chemo attractant protein-1 (MCP-1, CCL2), IFN-gamma-inducible protein 10 (IP- 10, CXCL10), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-α) were estimated both in circulation as well as in visceral adipose tissue by Milliplex Rat cytokine immunoassay kit (Millipore, USA) according to manufacturer’s instructions.

    Techniques: TBARS Assay

    BMP2 and BMP4 stimulate eNOS phosphorylation in PAECs. A and B , representative Western blots of bPAECs stimulated with BMP2 ( A ) or BMP4 ( B ) for various times and analyzed for eNOS phosphorylation at serine 1179. VEGF was used as a positive control. C

    Journal: The Journal of Biological Chemistry

    Article Title: Bone Morphogenetic Protein Receptor II Is a Novel Mediator of Endothelial Nitric-oxide Synthase Activation *

    doi: 10.1074/jbc.M111.274100

    Figure Lengend Snippet: BMP2 and BMP4 stimulate eNOS phosphorylation in PAECs. A and B , representative Western blots of bPAECs stimulated with BMP2 ( A ) or BMP4 ( B ) for various times and analyzed for eNOS phosphorylation at serine 1179. VEGF was used as a positive control. C

    Article Snippet: BMP4 and vascular endothelial growth factor (VEGF) were from R & D Systems (Minneapolis, MN).

    Techniques: Western Blot, Positive Control

    BMP2 and BMP4 stimulate eNOS enzymatic activity in PAECs. bPAECs were stimulated with BMP2, BMP4, or VEGF for 30 min, and lysates were analyzed for eNOS activity by measuring the conversion of arginine to citrulline. l -NAME added to the enzyme activity

    Journal: The Journal of Biological Chemistry

    Article Title: Bone Morphogenetic Protein Receptor II Is a Novel Mediator of Endothelial Nitric-oxide Synthase Activation *

    doi: 10.1074/jbc.M111.274100

    Figure Lengend Snippet: BMP2 and BMP4 stimulate eNOS enzymatic activity in PAECs. bPAECs were stimulated with BMP2, BMP4, or VEGF for 30 min, and lysates were analyzed for eNOS activity by measuring the conversion of arginine to citrulline. l -NAME added to the enzyme activity

    Article Snippet: BMP4 and vascular endothelial growth factor (VEGF) were from R & D Systems (Minneapolis, MN).

    Techniques: Activity Assay

    BMP2- and BMP4-stimulated eNOS phosphorylation is dependent on BMPRII. Serum-starved hPAECs transfected with control or BMPRII siRNA were stimulated for various times with BMP2, BMP4, or VEGF and then assayed for eNOS phosphorylation by Western blot analysis

    Journal: The Journal of Biological Chemistry

    Article Title: Bone Morphogenetic Protein Receptor II Is a Novel Mediator of Endothelial Nitric-oxide Synthase Activation *

    doi: 10.1074/jbc.M111.274100

    Figure Lengend Snippet: BMP2- and BMP4-stimulated eNOS phosphorylation is dependent on BMPRII. Serum-starved hPAECs transfected with control or BMPRII siRNA were stimulated for various times with BMP2, BMP4, or VEGF and then assayed for eNOS phosphorylation by Western blot analysis

    Article Snippet: BMP4 and vascular endothelial growth factor (VEGF) were from R & D Systems (Minneapolis, MN).

    Techniques: Transfection, Western Blot

    BMP2 and BMP4 stimulate eNOS-dependent PAEC migration. PAECs were stimulated with VEGF, BMP2, or BMP4 with or without l -NAME (100 μ m ) and assayed for migration using the monolayer wound assay. Data represent the mean ± S.D. ( error bars

    Journal: The Journal of Biological Chemistry

    Article Title: Bone Morphogenetic Protein Receptor II Is a Novel Mediator of Endothelial Nitric-oxide Synthase Activation *

    doi: 10.1074/jbc.M111.274100

    Figure Lengend Snippet: BMP2 and BMP4 stimulate eNOS-dependent PAEC migration. PAECs were stimulated with VEGF, BMP2, or BMP4 with or without l -NAME (100 μ m ) and assayed for migration using the monolayer wound assay. Data represent the mean ± S.D. ( error bars

    Article Snippet: BMP4 and vascular endothelial growth factor (VEGF) were from R & D Systems (Minneapolis, MN).

    Techniques: Migration

    BMP2 and BMP4 elicit canonical changes in eNOS protein-protein interactions. eNOS was immunoprecipitated ( IP ) from bPAECs stimulated for 30 min with BMP2, BMP4, or VEGF. Immunoprecipitates were subjected to SDS-PAGE and analyzed by Western blotting for

    Journal: The Journal of Biological Chemistry

    Article Title: Bone Morphogenetic Protein Receptor II Is a Novel Mediator of Endothelial Nitric-oxide Synthase Activation *

    doi: 10.1074/jbc.M111.274100

    Figure Lengend Snippet: BMP2 and BMP4 elicit canonical changes in eNOS protein-protein interactions. eNOS was immunoprecipitated ( IP ) from bPAECs stimulated for 30 min with BMP2, BMP4, or VEGF. Immunoprecipitates were subjected to SDS-PAGE and analyzed by Western blotting for

    Article Snippet: BMP4 and vascular endothelial growth factor (VEGF) were from R & D Systems (Minneapolis, MN).

    Techniques: Immunoprecipitation, SDS Page, Western Blot

    Activin promotes tumorigenicity of breast cancer cells in immunocompromised mice. ( a ) Stable overexpression of activin-A in MCF-7 enhances (i), whereas stable knockdown of activin-A in MDA-MB-231 cells (ii) reduces their tumor-forming ability in nude mice. Shown below are the representative images of the tumors formed s.c. ( b ) Immunohistochemical analysis of MCF-7-overexpressing tumors shows EMT-like changes and higher ki-67 index. ( c ) Treatment of MCF-7 cells with activin-A (i) or overexpression of activin-A in MCF-7 cells (ii) results in increased levels of vascular endothelial growth factor-A (VEGF-A). ( d ) Luciferase reporter assay in HEK 293T cells shows that activin-A regulates VEGF promoter activity. ( e ) Tail vein injection of activin-A-overexpressing MCF-7 cells shows better tumor-forming ability in the livers of nude mice (i). Normal is shown here as the reference from an animal without injection of any cells. The panel below (ii) shows the hematoxylin and eosin (H E) staining of the liver tissue sections. The graph (iii) and (iv) shows number and size of nodules formed in the liver per animal. ( f ) CD44 high/ CD24 low fluorescence-activated cell sorting (FACS) analysis of activin-A-overexpressing or knockdown cells shows that activin-A influences stemness of breast cancer cells (i). Quantitative PCR analysis shows that treatment of MCF-7 or MDA-MB-231 cells with recombinant activin-A induces various markers of stemness (ii).

    Journal: NPJ Breast Cancer

    Article Title: Activin-A signaling promotes epithelial–mesenchymal transition, invasion, and metastatic growth of breast cancer

    doi: 10.1038/npjbcancer.2015.7

    Figure Lengend Snippet: Activin promotes tumorigenicity of breast cancer cells in immunocompromised mice. ( a ) Stable overexpression of activin-A in MCF-7 enhances (i), whereas stable knockdown of activin-A in MDA-MB-231 cells (ii) reduces their tumor-forming ability in nude mice. Shown below are the representative images of the tumors formed s.c. ( b ) Immunohistochemical analysis of MCF-7-overexpressing tumors shows EMT-like changes and higher ki-67 index. ( c ) Treatment of MCF-7 cells with activin-A (i) or overexpression of activin-A in MCF-7 cells (ii) results in increased levels of vascular endothelial growth factor-A (VEGF-A). ( d ) Luciferase reporter assay in HEK 293T cells shows that activin-A regulates VEGF promoter activity. ( e ) Tail vein injection of activin-A-overexpressing MCF-7 cells shows better tumor-forming ability in the livers of nude mice (i). Normal is shown here as the reference from an animal without injection of any cells. The panel below (ii) shows the hematoxylin and eosin (H E) staining of the liver tissue sections. The graph (iii) and (iv) shows number and size of nodules formed in the liver per animal. ( f ) CD44 high/ CD24 low fluorescence-activated cell sorting (FACS) analysis of activin-A-overexpressing or knockdown cells shows that activin-A influences stemness of breast cancer cells (i). Quantitative PCR analysis shows that treatment of MCF-7 or MDA-MB-231 cells with recombinant activin-A induces various markers of stemness (ii).

    Article Snippet: Reagents Recombinant human activin-A (338-AC-010) and activin-A antibody (AF338) were purchased from R & D Systems (Minneapolis, MN, USA); phosphoSMAD2 (3101 and 9510) from Cell Signaling Technology (Boston, MA, USA); SMAD3 (1735), E-cadherin (1702), N-cadherin (2019), and α-smooth muscle actin (1184-1) from Epitomics (CA, USA); Vimentin (V2258) and fluorescein isothiocyanate-conjugated phalloidin (P5282) from Sigma (St Louis, MO, USA); phosphoSMAD3 (ab52903) and BMP2 (ab14933) from Abcam (Cambridge, MA, USA); vascular endothelial growth factor-A (VEGF-A) (M7273) from Dako (Denmark); and PE-CD44 (560533)/PE-cy7 CD24 (555428) from BD (NJ, USA).

    Techniques: Mouse Assay, Over Expression, Multiple Displacement Amplification, Immunohistochemistry, Luciferase, Reporter Assay, Activity Assay, Injection, Staining, Fluorescence, FACS, Real-time Polymerase Chain Reaction, Recombinant

    VEGF-A and -C protein expression of breast cancer MCF-7 cells in the COX-2-shRNA group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.

    Journal: Oncology Letters

    Article Title: Effect of cycloxygenase-2 silencing on the malignant biological behavior of MCF-7 breast cancer cells

    doi: 10.3892/ol.2014.2395

    Figure Lengend Snippet: VEGF-A and -C protein expression of breast cancer MCF-7 cells in the COX-2-shRNA group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.

    Article Snippet: Next, the primary rabbit monoclonal anti-COX-2 (1:500), anti-vascular endothelial growth factor (VEGF)-A (1:800), anti-VEGF-C (1:800) and anti-GAPDH (1:4,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) were added and the mixture was incubated overnight at 4°C on a rocking platform.

    Techniques: Expressing, shRNA

    Anti-VEGF mAb impairs tumor cell proliferation

    Journal: mAbs

    Article Title: VEGF neutralizing aerosol therapy in primary pulmonary adenocarcinoma with K-ras activating-mutations

    doi: 10.4161/mabs.34454

    Figure Lengend Snippet: Anti-VEGF mAb impairs tumor cell proliferation

    Article Snippet: Native or aerosolized G6–31 at 1, 5 or 10 nM in 1X PBS was pre-incubated with 0.5 nM of human VEGF-A (Cell Signaling) for 60 min, and then added to the cells.

    Techniques:

    VEGF neutralization impairs VEGFR2 activation

    Journal: mAbs

    Article Title: VEGF neutralizing aerosol therapy in primary pulmonary adenocarcinoma with K-ras activating-mutations

    doi: 10.4161/mabs.34454

    Figure Lengend Snippet: VEGF neutralization impairs VEGFR2 activation

    Article Snippet: Native or aerosolized G6–31 at 1, 5 or 10 nM in 1X PBS was pre-incubated with 0.5 nM of human VEGF-A (Cell Signaling) for 60 min, and then added to the cells.

    Techniques: Neutralization, Activation Assay

    Anti-VEGF mAb inhibits angiogenesis

    Journal: mAbs

    Article Title: VEGF neutralizing aerosol therapy in primary pulmonary adenocarcinoma with K-ras activating-mutations

    doi: 10.4161/mabs.34454

    Figure Lengend Snippet: Anti-VEGF mAb inhibits angiogenesis

    Article Snippet: Native or aerosolized G6–31 at 1, 5 or 10 nM in 1X PBS was pre-incubated with 0.5 nM of human VEGF-A (Cell Signaling) for 60 min, and then added to the cells.

    Techniques:

    Aerosolized anti-VEGF mAb passes slowly and poorly into the systemic circulation

    Journal: mAbs

    Article Title: VEGF neutralizing aerosol therapy in primary pulmonary adenocarcinoma with K-ras activating-mutations

    doi: 10.4161/mabs.34454

    Figure Lengend Snippet: Aerosolized anti-VEGF mAb passes slowly and poorly into the systemic circulation

    Article Snippet: Native or aerosolized G6–31 at 1, 5 or 10 nM in 1X PBS was pre-incubated with 0.5 nM of human VEGF-A (Cell Signaling) for 60 min, and then added to the cells.

    Techniques:

    NKP608 inhibited Wnt/β-catenin signaling pathway in colorectal cancer cells. a Western blot revealed that NKP608 resulted in an inhibitory action of Wnt relative proteins and proteins relevant cell growth including β-catenin, Wnt-3a, E-Cadherin, Cyclin D1 and VEGF. b Quantitative expression levels of proteins are shown. Values are expressed as the mean ± SD (n = 3). * p

    Journal: Biological Research

    Article Title: The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway

    doi: 10.1186/s40659-018-0163-x

    Figure Lengend Snippet: NKP608 inhibited Wnt/β-catenin signaling pathway in colorectal cancer cells. a Western blot revealed that NKP608 resulted in an inhibitory action of Wnt relative proteins and proteins relevant cell growth including β-catenin, Wnt-3a, E-Cadherin, Cyclin D1 and VEGF. b Quantitative expression levels of proteins are shown. Values are expressed as the mean ± SD (n = 3). * p

    Article Snippet: Antibodies to Active-Caspase-3, Wnt-3a, β-catenin, E-Cadherin, (vascular endothelial growth factor) VEGF were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Western Blot, Expressing

    Effect of simvastatin and bone marrow-derived MSCs on the expression of VEGF in ischemic limb . The levels of VEGF in ischemic limb 21 days after surgery is measured by Western blot. β-actin was used as an invariant control. * p

    Journal: Journal of Biomedical Science

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation

    doi: 10.1186/1423-0127-17-75

    Figure Lengend Snippet: Effect of simvastatin and bone marrow-derived MSCs on the expression of VEGF in ischemic limb . The levels of VEGF in ischemic limb 21 days after surgery is measured by Western blot. β-actin was used as an invariant control. * p

    Article Snippet: Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours.

    Techniques: Derivative Assay, Expressing, Western Blot

    Smad4 downregulates VEGF and inhibits angiogenesis. ( A ) VEGF expression in CT26 and SW620 cell clones was examined by western blot analyses. ( B ) Representative tumour masses with or without 5-FU treatment are shown. Visible vessels are marked by black arrows. Formalin-fixed paraffin-embedded tissue sections from ( C ) orthotopic tumours and ( D ) subcutaneous tumours were subjected to H E and CD31 staining. Representative pictures are shown ( × 100 and × 400) with vessel density and vessel number. * P

    Journal: British Journal of Cancer

    Article Title: Loss of Smad4 in colorectal cancer induces resistance to 5-fluorouracil through activating Akt pathway

    doi: 10.1038/bjc.2013.789

    Figure Lengend Snippet: Smad4 downregulates VEGF and inhibits angiogenesis. ( A ) VEGF expression in CT26 and SW620 cell clones was examined by western blot analyses. ( B ) Representative tumour masses with or without 5-FU treatment are shown. Visible vessels are marked by black arrows. Formalin-fixed paraffin-embedded tissue sections from ( C ) orthotopic tumours and ( D ) subcutaneous tumours were subjected to H E and CD31 staining. Representative pictures are shown ( × 100 and × 400) with vessel density and vessel number. * P

    Article Snippet: Antibodies were purchased as follows: Santa Cruz Biotechnology (Santa Cruz, CA, USA): anti-Smad4, anti-p21Cip1 , anti-p27Kip1 , anti-Cyclin D1, anti-Survivin, anti-Bcl-2, anti-VEGF; Cell Signaling (Denver, MA, USA): anti-PARP, anti-cleaved-Caspase3, anti-p-Akt, anti-Akt, anti-Bcl-w, anti-Bcl-xL, anti-Bad, anti-Bim, anti-Bax, anti-PUMA; Zymed Laboratories Inc. (San Francisco, CA, USA): anti-c-Myc.

    Techniques: Expressing, Clone Assay, Western Blot, Formalin-fixed Paraffin-Embedded, Staining

    CCL18 affects the expression levels of E-cadherin, MMP-2 and VEGF-C. (A and B) 5637 bladder cancer cells were treated with PBS or CCL18 (50 or 100 ng/ml) for 36 h, with or without pretreatment with the CCR8 inhibitor R243. Protein expression levels of E-cadherin, MMP-2 and VEGF-C were detected in 5637 cells by western blot analysis. *P

    Journal: Molecular Medicine Reports

    Article Title: CCL18 enhances migration, invasion and EMT by binding CCR8 in bladder cancer cells

    doi: 10.3892/mmr.2018.9791

    Figure Lengend Snippet: CCL18 affects the expression levels of E-cadherin, MMP-2 and VEGF-C. (A and B) 5637 bladder cancer cells were treated with PBS or CCL18 (50 or 100 ng/ml) for 36 h, with or without pretreatment with the CCR8 inhibitor R243. Protein expression levels of E-cadherin, MMP-2 and VEGF-C were detected in 5637 cells by western blot analysis. *P

    Article Snippet: Once proteins were transferred to nitrocellulose membranes, they were incubated with antibodies targeting β-actin (cat. no. 58169, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), CCR8 (cat. no. ab32131, 1:500; Abcam, Cambridge, UK), E-cadherin (cat. no. 3195, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), matrix metalloproteinase (MMP)-2 (cat. no. 40994, 1:1,000; Cell Signaling Technology, Inc.) and vascular endothelial growth factor (VEGF)-C (cat. no. 2445, 1:1,000; Cell Signaling Technology, Inc.), followed by incubation with a horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. ab6721, 1:3,000; Abcam) for 1.5 h at room temperature.

    Techniques: Expressing, Western Blot

    Effects of CCR8 shRNA on CCL18-induced expression of E-cadherin, MMP-2 and VEGF-C. 5637 bladder cancer cells were treated with CCL18 (50 ng/ml), CCL18 + NT shRNA or CCL18 + CCR8 shRNA for 36 h. (A) mRNA expression levels of CCR8 after being transfected with CCR8 shRNA was examined by reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal control. (B) mRNA expression levels of CCR8, E-cadherin, MMP-2 and VEGF-C were examined by reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal control. (C and D) Protein expression levels of CCR8, E-cadherin, MMP-2 and VEGF-C in 5637 cells were detected by western blotting. β-actin was used as an internal control. *P

    Journal: Molecular Medicine Reports

    Article Title: CCL18 enhances migration, invasion and EMT by binding CCR8 in bladder cancer cells

    doi: 10.3892/mmr.2018.9791

    Figure Lengend Snippet: Effects of CCR8 shRNA on CCL18-induced expression of E-cadherin, MMP-2 and VEGF-C. 5637 bladder cancer cells were treated with CCL18 (50 ng/ml), CCL18 + NT shRNA or CCL18 + CCR8 shRNA for 36 h. (A) mRNA expression levels of CCR8 after being transfected with CCR8 shRNA was examined by reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal control. (B) mRNA expression levels of CCR8, E-cadherin, MMP-2 and VEGF-C were examined by reverse transcription-quantitative polymerase chain reaction. β-actin was used as an internal control. (C and D) Protein expression levels of CCR8, E-cadherin, MMP-2 and VEGF-C in 5637 cells were detected by western blotting. β-actin was used as an internal control. *P

    Article Snippet: Once proteins were transferred to nitrocellulose membranes, they were incubated with antibodies targeting β-actin (cat. no. 58169, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), CCR8 (cat. no. ab32131, 1:500; Abcam, Cambridge, UK), E-cadherin (cat. no. 3195, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), matrix metalloproteinase (MMP)-2 (cat. no. 40994, 1:1,000; Cell Signaling Technology, Inc.) and vascular endothelial growth factor (VEGF)-C (cat. no. 2445, 1:1,000; Cell Signaling Technology, Inc.), followed by incubation with a horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. ab6721, 1:3,000; Abcam) for 1.5 h at room temperature.

    Techniques: shRNA, Expressing, Transfection, Real-time Polymerase Chain Reaction, Western Blot

    Mechanical ventilation increased MMP-7, cyclin D1, and VEGF in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to β-actin. Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p

    Journal: PLoS ONE

    Article Title: Activation of the Wnt/?-Catenin Signaling Pathway by Mechanical Ventilation Is Associated with Ventilator-Induced Pulmonary Fibrosis in Healthy Lungs

    doi: 10.1371/journal.pone.0023914

    Figure Lengend Snippet: Mechanical ventilation increased MMP-7, cyclin D1, and VEGF in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to β-actin. Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p

    Article Snippet: Detection of WNT5A, AXIN2 (Abcam, Cambridge, UK), total β-catenin, MMP-7, cyclin D1, and vascular endothelial growth factor (VEGF) (Santa Cruz Biotechnology, Santa Cruz, CA), non-phospho (Ser33/37/Thr41) β-catenin (Cell Signaling Technology, Massachusetts) were performed in random samples by Western blotting using rabbit polyclonal anti-WNT5A, anti-AXIN2, anti-β-catenin, anti-non-phospho (Ser33/37/Thr41) β-catenin, and anti-MMP-7 antibodies, and a goat anti-rabbit IgG-HRP as secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal antibody anti-cyclin D1 and a rabbit anti-mouse IgG-HRP as secondary antibody (Dako, Glostrup, Denmark), goat polyclonal anti-VEFG and a donkey anti-goat IgG-HRP (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques:

    Deletion of TXNIP does not alter VEGFR2/Akt activation under hyperoxia. Wild type (WT) and TXNIP knockout (TKO) mice were subjected to hyperoxia (75% O2, p7–p12). Activation of VEGFR2 ( A ) and Akt ( B ) were examined as downstream signal of VEGF in p12 WT and TKO retinas. TKO showed significant decrease in phosphorylation of VEGFR-2 and Akt compared to WT under normoxic condition. We did not detect significant change in the activation of VEGFR2 and its downstream Akt in retinas from TKO and WT in response to hyperoxia. (*P

    Journal: PLoS ONE

    Article Title: Deletion of Thioredoxin Interacting Protein (TXNIP) Augments Hyperoxia-Induced Vaso-Obliteration in a Mouse Model of Oxygen Induced-Retinopathy

    doi: 10.1371/journal.pone.0110388

    Figure Lengend Snippet: Deletion of TXNIP does not alter VEGFR2/Akt activation under hyperoxia. Wild type (WT) and TXNIP knockout (TKO) mice were subjected to hyperoxia (75% O2, p7–p12). Activation of VEGFR2 ( A ) and Akt ( B ) were examined as downstream signal of VEGF in p12 WT and TKO retinas. TKO showed significant decrease in phosphorylation of VEGFR-2 and Akt compared to WT under normoxic condition. We did not detect significant change in the activation of VEGFR2 and its downstream Akt in retinas from TKO and WT in response to hyperoxia. (*P

    Article Snippet: The primary antibodies were purchased as follow: VEGF (Rabbit polyclonal, EMD-Millipore), phosphor-VEGFR2, VEGFR2, phospho-Akt, Akt, phospho-ASK-1, ASK-1, cleaved caspase-3 (Rabbit polyclonal, Cell Signaling Tech, Danvers, MA), total Trx (Mouse monoclonal, Santa Cruz, Dallas, TX), and TXNIP (Rabbit polyclonal, Invitrogen, Grand Island, NY), cleaved PARP (BD Bioscience Pharmingen, San Diego, CA).

    Techniques: Activation Assay, Knock-Out, Mouse Assay

    Representative diagram shows the impact of TXNIP deletion on retina vasculature under both normoxia and hyperoxia. Under normoxia, retinas from TXNIP-deficient mice showed similar VEGF levels, less peroxynitrite (ONOO-) levels, less VEGF receptor-2 (pVEGFR2) activation and upregulated thioredoxin (Trx) that collectively lead to normal vascular development in comparison to WT mice. Under hyperoxia, retinas from WT mice showed higher peroxynitrite formation, less survival Akt activation (pAkt) and upregulated proapoptotic signal of ASK-1 resulting in vaso-obliteration. Retinas from TKO although showed less peroxynitrite levels and maintained Akt activation, retinas experienced significant decreases in thioredoxin (Trx) that shift the balance of the ASK-1-Trx inhibitory complex and increases the activation of the proapoptotic ASK-1 pathway leading to exacerbated vasoobliteration compared to WT.

    Journal: PLoS ONE

    Article Title: Deletion of Thioredoxin Interacting Protein (TXNIP) Augments Hyperoxia-Induced Vaso-Obliteration in a Mouse Model of Oxygen Induced-Retinopathy

    doi: 10.1371/journal.pone.0110388

    Figure Lengend Snippet: Representative diagram shows the impact of TXNIP deletion on retina vasculature under both normoxia and hyperoxia. Under normoxia, retinas from TXNIP-deficient mice showed similar VEGF levels, less peroxynitrite (ONOO-) levels, less VEGF receptor-2 (pVEGFR2) activation and upregulated thioredoxin (Trx) that collectively lead to normal vascular development in comparison to WT mice. Under hyperoxia, retinas from WT mice showed higher peroxynitrite formation, less survival Akt activation (pAkt) and upregulated proapoptotic signal of ASK-1 resulting in vaso-obliteration. Retinas from TKO although showed less peroxynitrite levels and maintained Akt activation, retinas experienced significant decreases in thioredoxin (Trx) that shift the balance of the ASK-1-Trx inhibitory complex and increases the activation of the proapoptotic ASK-1 pathway leading to exacerbated vasoobliteration compared to WT.

    Article Snippet: The primary antibodies were purchased as follow: VEGF (Rabbit polyclonal, EMD-Millipore), phosphor-VEGFR2, VEGFR2, phospho-Akt, Akt, phospho-ASK-1, ASK-1, cleaved caspase-3 (Rabbit polyclonal, Cell Signaling Tech, Danvers, MA), total Trx (Mouse monoclonal, Santa Cruz, Dallas, TX), and TXNIP (Rabbit polyclonal, Invitrogen, Grand Island, NY), cleaved PARP (BD Bioscience Pharmingen, San Diego, CA).

    Techniques: Mouse Assay, Activation Assay

    Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, Bcl‐2‐associated X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.

    Journal: Stem Cells Translational Medicine

    Article Title: Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA‐21

    doi: 10.5966/sctm.2015-0386

    Figure Lengend Snippet: Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, Bcl‐2‐associated X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.

    Article Snippet: After 1 hour of blocking with PBST containing 5% BSA at room temperature, primary antibodies, including anti‐Bcl‐2, Bcl‐2‐associated X protein (Bax), caspase‐3, phosphorylated‐Akt/total‐Akt, PTEN, vascular endothelial growth factor (VEGF), CD63, and β‐actin (Abcam) were diluted to 1:1,000 and incubated with the membrane overnight at 4°C.

    Techniques: Derivative Assay

    Kaplan-Meier curves of recurrence for the 2nd cohort of 47 CK20+ pN0 CRC patients after follow-up for 60 months based on VEGF-A mRNA copy numbers

    Journal: Oncotarget

    Article Title: Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients

    doi: 10.18632/oncotarget.23424

    Figure Lengend Snippet: Kaplan-Meier curves of recurrence for the 2nd cohort of 47 CK20+ pN0 CRC patients after follow-up for 60 months based on VEGF-A mRNA copy numbers

    Article Snippet: Validation of vascular endothelial growth factor A (VEGF-A) mRNA and transcription factor 20 (TCF20) mRNA in the second cohort of PELS TaqMan gene expression assays of specific primers and MGB probes were purchased from Applied Biosystems for the detection of VEGF-A mRNA (Hs00900054_m1) and TCF20 mRNA (Hs00390028_m1).

    Techniques:

    ( A ) VEGF-A mRNA and ( B ) TCF20 mRNA copy numbers per 0.05 μg total RNA in CK20+ and CK20- LNs from the 2nd cohort of pN0 CRC patients. The median in each group of subjects was indicated by a black horizontal line.

    Journal: Oncotarget

    Article Title: Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients

    doi: 10.18632/oncotarget.23424

    Figure Lengend Snippet: ( A ) VEGF-A mRNA and ( B ) TCF20 mRNA copy numbers per 0.05 μg total RNA in CK20+ and CK20- LNs from the 2nd cohort of pN0 CRC patients. The median in each group of subjects was indicated by a black horizontal line.

    Article Snippet: Validation of vascular endothelial growth factor A (VEGF-A) mRNA and transcription factor 20 (TCF20) mRNA in the second cohort of PELS TaqMan gene expression assays of specific primers and MGB probes were purchased from Applied Biosystems for the detection of VEGF-A mRNA (Hs00900054_m1) and TCF20 mRNA (Hs00390028_m1).

    Techniques:

    Western blot analysis of caspase-3 and VEGF in control and PEITC-treated tumors. Western blot analysis of caspase-3 and human VEGF were performed and quantitated as described in Materials and methods. Results were normalized to β-actin levels and expressed as means ± SE (n=4). (A) Caspase-3. (B) VEGF.

    Journal: International Journal of Oncology

    Article Title: Inhibition of androgen-responsive LNCaP prostate cancer cell tumor xenograft growth by dietary phenethyl isothiocyanate correlates with decreased angiogenesis and inhibition of cell attachment

    doi: 10.3892/ijo.2012.1335

    Figure Lengend Snippet: Western blot analysis of caspase-3 and VEGF in control and PEITC-treated tumors. Western blot analysis of caspase-3 and human VEGF were performed and quantitated as described in Materials and methods. Results were normalized to β-actin levels and expressed as means ± SE (n=4). (A) Caspase-3. (B) VEGF.

    Article Snippet: TaqMan gene expression assay primers and probes for human prostate specific antigen (PSA) and glyceraldehydes-3-phosphate dehydrogenase (G3PDH), proliferating cell nuclear antigen (PCNA), Ki-67, integrin β1 (ITGB1), integrin α2 (ITGA2), integrin α5 (ITGA5), integrin α6 (ITGA6), mouse and human vascular endothelial growth factor (VEGF) A, mouse PECAM-1 and mouse and human TATA binding protein were purchased from Applied Biosystems (Foster City, CA).

    Techniques: Western Blot

    Effect of BD,FO, FS and FSM given as diet twice daily for three weeks on VEGF expression in EAC-bearing mice. ( A ) Immunohistochemical staining of VEGF in EAC solid tumor sections (160×). BD (I), FO (II), FS (III) and FSM (IV). ( B ) Quantification of VEGF immunopositive cells to the total area of the microscopic field across six fields. Values were given as means ± SD (n = 6), a,b and c: Significantly different from BD, FO and FS respectively at p

    Journal: Scientific Reports

    Article Title: Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars

    doi: 10.1038/s41598-017-18944-0

    Figure Lengend Snippet: Effect of BD,FO, FS and FSM given as diet twice daily for three weeks on VEGF expression in EAC-bearing mice. ( A ) Immunohistochemical staining of VEGF in EAC solid tumor sections (160×). BD (I), FO (II), FS (III) and FSM (IV). ( B ) Quantification of VEGF immunopositive cells to the total area of the microscopic field across six fields. Values were given as means ± SD (n = 6), a,b and c: Significantly different from BD, FO and FS respectively at p

    Article Snippet: Assay of vascular endothelial growth factor (VEGF-A) level VEGF was determined in cell culture medium using eBioscience ELISA kit (San Diego, CA, USA).

    Techniques: Expressing, Mouse Assay, Immunohistochemistry, Staining

    Effect of treatment of T47D cells with IC 50 of PFH-G9 (15 μg/ml) or TAM (15 μM) for 48 h on VEGF level. Each point is the mean ± SD of three independent experiments performed in duplicate. a: Significantly different from control group at p

    Journal: Scientific Reports

    Article Title: Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars

    doi: 10.1038/s41598-017-18944-0

    Figure Lengend Snippet: Effect of treatment of T47D cells with IC 50 of PFH-G9 (15 μg/ml) or TAM (15 μM) for 48 h on VEGF level. Each point is the mean ± SD of three independent experiments performed in duplicate. a: Significantly different from control group at p

    Article Snippet: Assay of vascular endothelial growth factor (VEGF-A) level VEGF was determined in cell culture medium using eBioscience ELISA kit (San Diego, CA, USA).

    Techniques:

    Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and VEGF-A ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon γ-induced protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.

    Journal: Scientific Reports

    Article Title: Elevated serum interferon γ-induced protein 10 kDa is associated with TAFRO syndrome

    doi: 10.1038/srep42316

    Figure Lengend Snippet: Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and VEGF-A ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon γ-induced protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.

    Article Snippet: Measurement of cytokines and chemokines Serum cytokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-22, IL-23, IL-27, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, platelet-derived growth factor (PDGF)-AA, vascular endothelial growth factor (VEGF)-A, and chemokine interferon γ-induced protein 10 kDa (IP-10) were analyzed using a FlowCytomix™ kit (Bender MedSystems, Vienna, Austria) and MACSQuant® Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturers’ instructions.

    Techniques: Derivative Assay

    Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p

    Journal: Frontiers in Oncology

    Article Title: Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells

    doi: 10.3389/fonc.2017.00182

    Figure Lengend Snippet: Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p

    Article Snippet: Materials VEGF-A (Sigma-Aldrich, V4512, USA) and axitinib (Selleckchem, S1005, USA) was added to cell culture medium in a concentration of 0.1 and 10 µg/ml, respectively.

    Techniques: Irradiation, Chemotaxis Assay, Migration, Software, Inhibition

    Expression of vascular endothelial growth factor (VEGF)-R2 ( KDR ) in U-251 and U-373 glioblastoma cell lines. (A,B) VEGF-R1 ( FLT 1) as well as VEGF-R2 ( KDR) are expressed in both cell lines U-251 (A) and U-373 (B) . β -Actin was used as housekeeping gene. PCR products were isolated and confirmed by DNA sequencing. (C,D) Sequence of amplified PCR product for FLT1 (C) and KDR (D) matches their specific database entries ( FLT1 – NM_002019.4; KDR – NM_002253.2) and were proven to be unique in human glioblastoma cell lines U-251 as well as in U-373 by comparison with a database (Blast 2.2, U.S. National Centre for Biotechnology Information, Bethesda, MD, USA) (E) . Semi-quantitative analysis of gene expression normalized to β -Actin and compared to the expression of U-251-FLT (100%). Data are shown as mean ± SEM. n = 3. (F) Both glioblastoma multiforme cell lines express VEGF-R2 (green dots, arrows) in the cytoplasm as well as along the cell membrane. Counterstaining of the actin cytoskeleton is given in red as well as cell nuclei staining with DAPI in blue. Scale bars: 10 µm.

    Journal: Frontiers in Oncology

    Article Title: Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells

    doi: 10.3389/fonc.2017.00182

    Figure Lengend Snippet: Expression of vascular endothelial growth factor (VEGF)-R2 ( KDR ) in U-251 and U-373 glioblastoma cell lines. (A,B) VEGF-R1 ( FLT 1) as well as VEGF-R2 ( KDR) are expressed in both cell lines U-251 (A) and U-373 (B) . β -Actin was used as housekeeping gene. PCR products were isolated and confirmed by DNA sequencing. (C,D) Sequence of amplified PCR product for FLT1 (C) and KDR (D) matches their specific database entries ( FLT1 – NM_002019.4; KDR – NM_002253.2) and were proven to be unique in human glioblastoma cell lines U-251 as well as in U-373 by comparison with a database (Blast 2.2, U.S. National Centre for Biotechnology Information, Bethesda, MD, USA) (E) . Semi-quantitative analysis of gene expression normalized to β -Actin and compared to the expression of U-251-FLT (100%). Data are shown as mean ± SEM. n = 3. (F) Both glioblastoma multiforme cell lines express VEGF-R2 (green dots, arrows) in the cytoplasm as well as along the cell membrane. Counterstaining of the actin cytoskeleton is given in red as well as cell nuclei staining with DAPI in blue. Scale bars: 10 µm.

    Article Snippet: Materials VEGF-A (Sigma-Aldrich, V4512, USA) and axitinib (Selleckchem, S1005, USA) was added to cell culture medium in a concentration of 0.1 and 10 µg/ml, respectively.

    Techniques: Expressing, Polymerase Chain Reaction, Isolation, DNA Sequencing, Sequencing, Amplification, Staining

    Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the proliferation of glioblastoma multiforme cell lines U-251 and U-373. (A,B) Axitinib impairs the proliferation of irradiated and non-irradiated U-251 and U-373 cells. VEGF, irradiation, or the combination of both has no significant effect on cell proliferation. The proliferation of the cells was analyzed by a modified MTS-test. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by *** p

    Journal: Frontiers in Oncology

    Article Title: Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells

    doi: 10.3389/fonc.2017.00182

    Figure Lengend Snippet: Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the proliferation of glioblastoma multiforme cell lines U-251 and U-373. (A,B) Axitinib impairs the proliferation of irradiated and non-irradiated U-251 and U-373 cells. VEGF, irradiation, or the combination of both has no significant effect on cell proliferation. The proliferation of the cells was analyzed by a modified MTS-test. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by *** p

    Article Snippet: Materials VEGF-A (Sigma-Aldrich, V4512, USA) and axitinib (Selleckchem, S1005, USA) was added to cell culture medium in a concentration of 0.1 and 10 µg/ml, respectively.

    Techniques: Irradiation, Modification

    Potential signaling in human glioblastoma cell lines after treatment with vascular endothelial growth factor (VEGF), irradiation and axitinib. VEGF activates multiple pathways including the Cdc42 and PLC pathways concerning cell migration and proliferation. It is supposed that stimulating effects of irradiation are mediated via enhanced synthesis of VEGF. Irradiation elevates VEGF biosynthesis of glioblastoma multiforme cells via MAPK activation. (A) Activation of the Cdc42 pathway by VEGF leads to an increased activation of Arp 2/3, HSP27, and ADF/cofilin resulting in an enhanced motility. Blockade of the VEGF-R2 by axitinib might decrease the activation of the Cdc42 pathway due to theoretical consideration resulting in a crucial decreased cell motility. (B) VEGF activates the PLC pathway which is involved in cell proliferation while axitinib might deactivate this pathway with impairment of cell proliferation. While motility is increased by VEGF, no positive effect on proliferation could be observed. This might be due to fully activated VEGF-pathways even under control conditions. Continuous arrows: evidence of the effect, dotted arrows: assumption of the effect.

    Journal: Frontiers in Oncology

    Article Title: Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells

    doi: 10.3389/fonc.2017.00182

    Figure Lengend Snippet: Potential signaling in human glioblastoma cell lines after treatment with vascular endothelial growth factor (VEGF), irradiation and axitinib. VEGF activates multiple pathways including the Cdc42 and PLC pathways concerning cell migration and proliferation. It is supposed that stimulating effects of irradiation are mediated via enhanced synthesis of VEGF. Irradiation elevates VEGF biosynthesis of glioblastoma multiforme cells via MAPK activation. (A) Activation of the Cdc42 pathway by VEGF leads to an increased activation of Arp 2/3, HSP27, and ADF/cofilin resulting in an enhanced motility. Blockade of the VEGF-R2 by axitinib might decrease the activation of the Cdc42 pathway due to theoretical consideration resulting in a crucial decreased cell motility. (B) VEGF activates the PLC pathway which is involved in cell proliferation while axitinib might deactivate this pathway with impairment of cell proliferation. While motility is increased by VEGF, no positive effect on proliferation could be observed. This might be due to fully activated VEGF-pathways even under control conditions. Continuous arrows: evidence of the effect, dotted arrows: assumption of the effect.

    Article Snippet: Materials VEGF-A (Sigma-Aldrich, V4512, USA) and axitinib (Selleckchem, S1005, USA) was added to cell culture medium in a concentration of 0.1 and 10 µg/ml, respectively.

    Techniques: Irradiation, Planar Chromatography, Migration, Activation Assay

    Expression of homing and angiogenic signals of the myocardium 7 days after cardiac transfer of green fluorescent protein (GFP)-Luc-mesenchymal stem cell (MSCs). Fluorescent immunohistochemistry of the bioluminescence positive myocardial areas 7 days after intramyocardial [left panel (A,C,E,G) ] or intracoronary [right panel (B,D,F,H) ] GFP-Luc-MSCs delivery shows increased expression of homing signals cadherin (A,B) , and angiogenic factors fibroblast growth factor 2 (FGF2) (C,D) and vascular endothelial growth factor (VEGF) (E,F) in group IM. Infarct area border zone (G,H) exhibited higher number of myocardial cells and higher level of VEGF expression in group IM (G) . Hoechst staining of the nuclei, 40× magnification. Expression of homing signals cadherin (I) , stromal-derived factor-1alpha (J) , and angiogenic factors FGF2 (K) and VEGF (L) .

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Matrix Metalloproteinase-2 Impairs Homing of Intracoronary Delivered Mesenchymal Stem Cells in a Porcine Reperfused Myocardial Infarction: Comparison With Intramyocardial Cell Delivery

    doi: 10.3389/fbioe.2018.00035

    Figure Lengend Snippet: Expression of homing and angiogenic signals of the myocardium 7 days after cardiac transfer of green fluorescent protein (GFP)-Luc-mesenchymal stem cell (MSCs). Fluorescent immunohistochemistry of the bioluminescence positive myocardial areas 7 days after intramyocardial [left panel (A,C,E,G) ] or intracoronary [right panel (B,D,F,H) ] GFP-Luc-MSCs delivery shows increased expression of homing signals cadherin (A,B) , and angiogenic factors fibroblast growth factor 2 (FGF2) (C,D) and vascular endothelial growth factor (VEGF) (E,F) in group IM. Infarct area border zone (G,H) exhibited higher number of myocardial cells and higher level of VEGF expression in group IM (G) . Hoechst staining of the nuclei, 40× magnification. Expression of homing signals cadherin (I) , stromal-derived factor-1alpha (J) , and angiogenic factors FGF2 (K) and VEGF (L) .

    Article Snippet: To investigate the homing of the injected GFP-Luc-MSCs, myocardial expression of cadherin (Abcam, Cambridge, UK), and for angiogenic factors, vascular endothelial growth factor (VEGF) (Cusabio, Cologne, Germany) and FGF2 (Lifespan, Seattle, WA, USA) were displayed by immunofluorescence staining.

    Techniques: Expressing, Immunohistochemistry, Staining, Derivative Assay