variable regions v3 Search Results


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  • 88
    Roche variable v3 region
    Variable V3 Region, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche v3 5 16s variable region sequences
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    Illumina Inc 16s rrna gene v1 v3 variable region pcr primers 27f 5
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    Illumina Inc 600 base v3 reagent kit
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    Bio-Rad 16s rrna gene
    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total <t>16S</t> <t>rRNA,</t> organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p
    16s Rrna Gene, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc miseq v2 platform
    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total <t>16S</t> <t>rRNA,</t> organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p
    Miseq V2 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc 16s library preparation
    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total <t>16S</t> <t>rRNA,</t> organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p
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    Roche 454 gs flx titanium sequencer
    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total <t>16S</t> <t>rRNA,</t> organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p
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    Qiagen taq pcr core
    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total <t>16S</t> <t>rRNA,</t> organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p
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    Image Search Results


    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total 16S rRNA, organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p

    Journal: PLoS ONE

    Article Title: Microbiota-Independent Ameliorative Effects of Antibiotics on Spontaneous Th2-Associated Pathology of the Small Intestine

    doi: 10.1371/journal.pone.0118795

    Figure Lengend Snippet: TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine. (A) Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. (B) Heatmap analysis shows relative abundance of taxa as a percentage of total 16S rRNA, organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). (C) The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p

    Article Snippet: Briefly, the target region of the 16S rRNA gene (V1–V3 of variable region) was amplified by using barcoded fusion primers with a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA).

    Techniques: Mouse Assay, Isolation