Journal: bioRxiv

Article Title: Repurposing Antibacterial Drugs and Clinical Molecules to Combat Vancomycin-Resistant Enterococci (VRE)

doi: 10.1101/2025.03.27.645859

Figure Lengend Snippet: Time kill assay of ridinilazole, CRS3123 and linezolid at 5× and 10× MIC, against E. faecium NR-31909. Samples treated with DMSO were used as negative control. The results are given as means ± SD (n = 3; data without error bars indicate that the SD is too small to be seen).

Article Snippet: Linezolid (Selleck Chemicals) and vancomycin hydrochloride (Gold Biotechnology) were all purchased from commercial suppliers.

Techniques: Time-Kill Assay, Negative Control

Journal: bioRxiv

Article Title: Repurposing Antibacterial Drugs and Clinical Molecules to Combat Vancomycin-Resistant Enterococci (VRE)

doi: 10.1101/2025.03.27.645859

Figure Lengend Snippet: In vivo efficacy of ridinilazole and CRS3123 in a Caenorhabditis elegans model of bacterial infection. C. elegans were infected with VRE , E. faecium NR-31909. After infection, worms were treated with ridinilazole and CRS3123 or linezolid at 10× MIC. After 24 hours, worms were lysed, and bacteria were plated and CFU were counted after 24 hr. Results are expressed as means from three biological replicates ± standard deviation. Statistical analyses were determined by one-way ANOVA with post hoc testing (*** p <0.005), (**** p < 0.0001).

Article Snippet: Linezolid (Selleck Chemicals) and vancomycin hydrochloride (Gold Biotechnology) were all purchased from commercial suppliers.

Techniques: In Vivo, Infection, Bacteria, Standard Deviation

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: In vitro release of hydrophobic drugs by oleogel rods with biocompatible gelators

doi: 10.1016/j.ejps.2020.105413

Figure Lengend Snippet: Summary of formulations used in drug release.

Article Snippet: The drug dexamethasone (purity: 99%), triamcinolone (purity 97%), and vancomycin HCL (vancomycin) (purity: 93.9%) were obtained from Carbosynth Limited.

Techniques: Solubility

Journal: Journal of Biological Chemistry

Article Title: Helix 8 of the Viral Chemokine Receptor ORF74 Directs Chemokine Binding

doi: 10.1074/jbc.m606877200

Figure Lengend Snippet: FIGURE 1. Expression of ORF74 deletion mutants. A, schematic representa- tion of the C-tail of ORF74-WT and mutants. Residues downstream of the conserved Pro at the end of TM7 (P7.50311) are shown. R7.61322 and Q7.62323 are shown in bold. The region conserved among -herpesvirus GPCRs is depicted in italics, and Hx8 is underlined. B, ELISA with HA-tagged. COS-7 cells were transfected with cDNA encoding N-terminally HA-tagged ORF74 con- structsoremptyvector.Forty-eighthoursaftertransfection,expressionofthe HA-tagged receptors was determined using an anti-HA antibody in an ELISA. Data are presented as the percentage of the absorption displayed by mock- transfected (mock) cells. A representative experiment performed in triplicate is shown. The experiment was repeated three times. C, confocal microscopy with EGFP-tagged ORF74. Representative pictures of COS-7 cells were made after transfection with C-terminally EGFP-tagged ORF74 constructs.

Article Snippet: After blocking with 1% skimmilk in 0.1 M NaHCO3 (pH 8.6), cells were incubated with mouse monoclonal anti-HA antibody (a gift from J. van Minnen) in Tris-buffered saline containing 0.1% bovine serum albumin, washed three times with Tris-buffered saline, and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Bio-Rad).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Confocal Microscopy, Construct