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  • 99
    Millipore guinea pig anti vacht
    Signature anatomical and physiological characteristics of <t>GFP</t> tagged On-Off DSGCs Flat mount retina with a , GFP + On-Off DSGCs. b , co-stained with dapi. c , Positions of GFP + RGCs. Scale in c , 150 µm. d–f , High magnification views. Scale, 12 µm. g , Targeted fill of a GFP + DSGC. Scale, 50 µm. h , Schematic of On-Off DSGC stratification and starburst amacrine cells (magenta). Labeling as in Extended Fig. 1 . i,j , Higher magnification of framed region in g stained for <t>VAChT</t> (starburst amacrine processes). Asterisk: ‘looping arborizations’. Dashed line: GFP arbor, which matches VAChT plexus. Scale, 10 µm. k,l , Side (x-z plane) views of cell in ( g ). GFP + dendrites co-stratify with both the On and Off sublayers. Scale, 5 µm. m , Direction-tuned response of a GFP + On-Off DSGC targeted for recording and receptive field characterization. The spike count is highest for bars moving toward ~270° in the cardinal axes.
    Guinea Pig Anti Vacht, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore vacht
    Z-projections of confocal micrographs of SNAP25-ir (red) double-labeled with either <t>ChT1-ir</t> (A) or <t>VAChT-ir</t> (B) on midmodiolar sections of midbasal regions of the rat cochlea at P21. (A) ChT1-ir (green) colocalizes with most but not all of the SNAP25-ir
    Vacht, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Synaptic Systems rabbit anti vacht
    Direct conversion of human astrocytes into MN-like cells using the small-molecule cocktail. a and b Immunocytochemical analysis of induced neurons for the expression of the neuronal marker TUJ1 and the MN-specific markers HB9 and islet 1 <t>(ISL1)</t> after 10–14 days of chemical induction. Scale bars = 25 μm. c Immunostaining assays for the expression of tyrosine hydroxylase (TH), γ-aminobutyric acid (GABA), and vesicular glutamate transporter 1 (vGlut1) in the induced cells. Scale bars = 25 μm. d - g Immunostaining assays for choline acetyltransferase (CHAT), vesicular acetylcholine transporter <t>(VAChT),</t> neuronal nuclei (NeuN), and synapsin-1 (SYN) after 14 days of chemical induction. d , e , f , scale bars = 25 μm. g , scale bar = 50 μm. h Expression of CHAT and VAChT in control HA1800 astrocytes. Scale bars = 25 μm. i The percentage of TUJ1 + cells compared to the that of total DAPI + cells after 2 weeks of induction (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples). j The percentages of TUJ1 + HB9 + and TUJ1 + ISL1 + cells compared to the total DAPI + cells after 2 weeks of induction (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples). k The percentages of TUJ1 + HB9 + , TUJ1 + ISL1 + , and TUJ1 + CHAT + cells relative to that of TUJ1 + cells induced by small molecules (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples)
    Rabbit Anti Vacht, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore rabbit anti vacht
    Direct conversion of human astrocytes into MN-like cells using the small-molecule cocktail. a and b Immunocytochemical analysis of induced neurons for the expression of the neuronal marker TUJ1 and the MN-specific markers HB9 and islet 1 <t>(ISL1)</t> after 10–14 days of chemical induction. Scale bars = 25 μm. c Immunostaining assays for the expression of tyrosine hydroxylase (TH), γ-aminobutyric acid (GABA), and vesicular glutamate transporter 1 (vGlut1) in the induced cells. Scale bars = 25 μm. d - g Immunostaining assays for choline acetyltransferase (CHAT), vesicular acetylcholine transporter <t>(VAChT),</t> neuronal nuclei (NeuN), and synapsin-1 (SYN) after 14 days of chemical induction. d , e , f , scale bars = 25 μm. g , scale bar = 50 μm. h Expression of CHAT and VAChT in control HA1800 astrocytes. Scale bars = 25 μm. i The percentage of TUJ1 + cells compared to the that of total DAPI + cells after 2 weeks of induction (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples). j The percentages of TUJ1 + HB9 + and TUJ1 + ISL1 + cells compared to the total DAPI + cells after 2 weeks of induction (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples). k The percentages of TUJ1 + HB9 + , TUJ1 + ISL1 + , and TUJ1 + CHAT + cells relative to that of TUJ1 + cells induced by small molecules (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples)
    Rabbit Anti Vacht, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    The VAChT SLC18A3 Antibody from Novus Biologicals is a sheep polyclonal antibody to VAChT SLC18A3 This antibody reacts with rat guinea pig The VAChT SLC18A3 Antibody has been validated for
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    Image Search Results


    Signature anatomical and physiological characteristics of GFP tagged On-Off DSGCs Flat mount retina with a , GFP + On-Off DSGCs. b , co-stained with dapi. c , Positions of GFP + RGCs. Scale in c , 150 µm. d–f , High magnification views. Scale, 12 µm. g , Targeted fill of a GFP + DSGC. Scale, 50 µm. h , Schematic of On-Off DSGC stratification and starburst amacrine cells (magenta). Labeling as in Extended Fig. 1 . i,j , Higher magnification of framed region in g stained for VAChT (starburst amacrine processes). Asterisk: ‘looping arborizations’. Dashed line: GFP arbor, which matches VAChT plexus. Scale, 10 µm. k,l , Side (x-z plane) views of cell in ( g ). GFP + dendrites co-stratify with both the On and Off sublayers. Scale, 5 µm. m , Direction-tuned response of a GFP + On-Off DSGC targeted for recording and receptive field characterization. The spike count is highest for bars moving toward ~270° in the cardinal axes.

    Journal: Nature

    Article Title: A dedicated circuit linking direction selective retinal ganglion cells to primary visual cortex

    doi: 10.1038/nature12989

    Figure Lengend Snippet: Signature anatomical and physiological characteristics of GFP tagged On-Off DSGCs Flat mount retina with a , GFP + On-Off DSGCs. b , co-stained with dapi. c , Positions of GFP + RGCs. Scale in c , 150 µm. d–f , High magnification views. Scale, 12 µm. g , Targeted fill of a GFP + DSGC. Scale, 50 µm. h , Schematic of On-Off DSGC stratification and starburst amacrine cells (magenta). Labeling as in Extended Fig. 1 . i,j , Higher magnification of framed region in g stained for VAChT (starburst amacrine processes). Asterisk: ‘looping arborizations’. Dashed line: GFP arbor, which matches VAChT plexus. Scale, 10 µm. k,l , Side (x-z plane) views of cell in ( g ). GFP + dendrites co-stratify with both the On and Off sublayers. Scale, 5 µm. m , Direction-tuned response of a GFP + On-Off DSGC targeted for recording and receptive field characterization. The spike count is highest for bars moving toward ~270° in the cardinal axes.

    Article Snippet: The following primary and secondary antibodies were used: rabbit anti-GFP (1:1000, Invitrogen), guinea pig anti-VAChT (1:1000, Millipore), Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 647 goat anti-guinea pig (1:1000, Invitrogen).

    Techniques: Staining, Labeling

    Z-projections of confocal micrographs of SNAP25-ir (red) double-labeled with either ChT1-ir (A) or VAChT-ir (B) on midmodiolar sections of midbasal regions of the rat cochlea at P21. (A) ChT1-ir (green) colocalizes with most but not all of the SNAP25-ir

    Journal:

    Article Title: The Final Stage of Cholinergic Differentiation Occurs Below Inner Hair Cells During Development of the Rodent Cochlea

    doi: 10.1007/s10162-005-0018-3

    Figure Lengend Snippet: Z-projections of confocal micrographs of SNAP25-ir (red) double-labeled with either ChT1-ir (A) or VAChT-ir (B) on midmodiolar sections of midbasal regions of the rat cochlea at P21. (A) ChT1-ir (green) colocalizes with most but not all of the SNAP25-ir

    Article Snippet: Samples were blocked with 10% normal goat serum (NGS) in 0.1 M PBS containing 1% Triton X-100 and then incubated with antibodies against cholinergic markers: ChT1, VAChT (antirabbit, 1:1000; Chemicon); and axon terminal markers: GAP43 (antimouse, 1:500; Sigma, St. Louis, MO), Synapsin I (antirabbit, 1:500; Chemicon), or SNAP-25 (antimouse, 1:2000; BD Transductions, Palo Alto, CA, USA).

    Techniques: Labeling

    Confocal micrographs of ChT1-ir (A, C) and VAChT-ir (B, D) on serial midmodiolar sections of midbasal regions of mouse cochlea at P2 and P4. (A) ChT1-ir (curved arrow) was just detectable below IHCs. This animal was the only example ( n = 10) of ChT1-ir

    Journal:

    Article Title: The Final Stage of Cholinergic Differentiation Occurs Below Inner Hair Cells During Development of the Rodent Cochlea

    doi: 10.1007/s10162-005-0018-3

    Figure Lengend Snippet: Confocal micrographs of ChT1-ir (A, C) and VAChT-ir (B, D) on serial midmodiolar sections of midbasal regions of mouse cochlea at P2 and P4. (A) ChT1-ir (curved arrow) was just detectable below IHCs. This animal was the only example ( n = 10) of ChT1-ir

    Article Snippet: Samples were blocked with 10% normal goat serum (NGS) in 0.1 M PBS containing 1% Triton X-100 and then incubated with antibodies against cholinergic markers: ChT1, VAChT (antirabbit, 1:1000; Chemicon); and axon terminal markers: GAP43 (antimouse, 1:500; Sigma, St. Louis, MO), Synapsin I (antirabbit, 1:500; Chemicon), or SNAP-25 (antimouse, 1:2000; BD Transductions, Palo Alto, CA, USA).

    Techniques:

    Confocal micrographs of ChT1-ir, synapsin-ir, and VAChT-ir on consecutive midmodiolar sections of midbasal regions of mouse cochlea. In the P20 (A–C) and P50 (D–F) mouse cochlea, ChT1-ir (A, D) was almost exclusively localized below OHCs,

    Journal:

    Article Title: The Final Stage of Cholinergic Differentiation Occurs Below Inner Hair Cells During Development of the Rodent Cochlea

    doi: 10.1007/s10162-005-0018-3

    Figure Lengend Snippet: Confocal micrographs of ChT1-ir, synapsin-ir, and VAChT-ir on consecutive midmodiolar sections of midbasal regions of mouse cochlea. In the P20 (A–C) and P50 (D–F) mouse cochlea, ChT1-ir (A, D) was almost exclusively localized below OHCs,

    Article Snippet: Samples were blocked with 10% normal goat serum (NGS) in 0.1 M PBS containing 1% Triton X-100 and then incubated with antibodies against cholinergic markers: ChT1, VAChT (antirabbit, 1:1000; Chemicon); and axon terminal markers: GAP43 (antimouse, 1:500; Sigma, St. Louis, MO), Synapsin I (antirabbit, 1:500; Chemicon), or SNAP-25 (antimouse, 1:2000; BD Transductions, Palo Alto, CA, USA).

    Techniques:

    Direct conversion of human astrocytes into MN-like cells using the small-molecule cocktail. a and b Immunocytochemical analysis of induced neurons for the expression of the neuronal marker TUJ1 and the MN-specific markers HB9 and islet 1 (ISL1) after 10–14 days of chemical induction. Scale bars = 25 μm. c Immunostaining assays for the expression of tyrosine hydroxylase (TH), γ-aminobutyric acid (GABA), and vesicular glutamate transporter 1 (vGlut1) in the induced cells. Scale bars = 25 μm. d - g Immunostaining assays for choline acetyltransferase (CHAT), vesicular acetylcholine transporter (VAChT), neuronal nuclei (NeuN), and synapsin-1 (SYN) after 14 days of chemical induction. d , e , f , scale bars = 25 μm. g , scale bar = 50 μm. h Expression of CHAT and VAChT in control HA1800 astrocytes. Scale bars = 25 μm. i The percentage of TUJ1 + cells compared to the that of total DAPI + cells after 2 weeks of induction (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples). j The percentages of TUJ1 + HB9 + and TUJ1 + ISL1 + cells compared to the total DAPI + cells after 2 weeks of induction (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples). k The percentages of TUJ1 + HB9 + , TUJ1 + ISL1 + , and TUJ1 + CHAT + cells relative to that of TUJ1 + cells induced by small molecules (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples)

    Journal: Military Medical Research

    Article Title: Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules

    doi: 10.1186/s40779-020-00271-7

    Figure Lengend Snippet: Direct conversion of human astrocytes into MN-like cells using the small-molecule cocktail. a and b Immunocytochemical analysis of induced neurons for the expression of the neuronal marker TUJ1 and the MN-specific markers HB9 and islet 1 (ISL1) after 10–14 days of chemical induction. Scale bars = 25 μm. c Immunostaining assays for the expression of tyrosine hydroxylase (TH), γ-aminobutyric acid (GABA), and vesicular glutamate transporter 1 (vGlut1) in the induced cells. Scale bars = 25 μm. d - g Immunostaining assays for choline acetyltransferase (CHAT), vesicular acetylcholine transporter (VAChT), neuronal nuclei (NeuN), and synapsin-1 (SYN) after 14 days of chemical induction. d , e , f , scale bars = 25 μm. g , scale bar = 50 μm. h Expression of CHAT and VAChT in control HA1800 astrocytes. Scale bars = 25 μm. i The percentage of TUJ1 + cells compared to the that of total DAPI + cells after 2 weeks of induction (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples). j The percentages of TUJ1 + HB9 + and TUJ1 + ISL1 + cells compared to the total DAPI + cells after 2 weeks of induction (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples). k The percentages of TUJ1 + HB9 + , TUJ1 + ISL1 + , and TUJ1 + CHAT + cells relative to that of TUJ1 + cells induced by small molecules (means ± SEM, n = 10 randomly selected 20× fields from triplicate samples)

    Article Snippet: The following primary antibodies were used: rabbit anti-GFAP (1:500, Abcam), mouse anti-GFAP (1:100, Santa Cruz), mouse anti-TUJ1 (1:500, Covance), rabbit anti-TUJ1(1:500, Sigma), mouse anti-O4 (1:200, Millipore), rabbit-anti MAP2 (1:500, Millipore), mouse anti-HB9 [1:50, 81.5C10, Developmental Studies Hybridoma Bank (DSHB)], rabbit anti-HB9 (1:200, Abcam), mouse anti-ISL1 (1:100, DSHB), rabbit anti-ISL1 (1:200, Abcam), goat anti-CHAT (1:200, Millipore), rabbit anti-VAChT (1:1000, SYSY), rabbit ani-GABA (1:500, Sigma), rabbit anti-vGlut1 (1:1000, Invitrogen), mouse anti-TH (1:200, Millipore), mouse anti-NeuN (1:200, Millipore), rabbit anti-SYN (1:500, Millipore), rabbit anti-SOX2 (1:200, Millipore), mouse anti-NESTIN (1:200, Millipore), rabbit anti-PAX6 (1:500, Biolegend), rabbit anti-Ki67 (1:500, ab15580, Abcam), and mouse anti-BrdU (1:50, 2750, Millipore).

    Techniques: Expressing, Marker, Immunostaining