v4 hypervariable region Search Results


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  • 93
    Illumina Inc v4 hypervariable region
    Pictorial representation of expected versus the actual relative abundance observed for the individual components of the mock bacterial community on the MiSeq and Roche454 GS Junior platform for primer pair targeting the ( a ) V1-V3 hypervariable region and ( b ) V3-V4 hypervariable region. Note: In the case of Pseudomonadaceae the MiSeq was able to identify the bacterial component at very low abundance
    V4 Hypervariable Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pacific Biosciences v4 hypervariable regions
    Pictorial representation of expected versus the actual relative abundance observed for the individual components of the mock bacterial community on the MiSeq and Roche454 GS Junior platform for primer pair targeting the ( a ) V1-V3 hypervariable region and ( b ) V3-V4 hypervariable region. Note: In the case of Pseudomonadaceae the MiSeq was able to identify the bacterial component at very low abundance
    V4 Hypervariable Regions, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Illumina Inc v3 v4 hypervariable region
    Relative abundance of bacterial 16S rRNA gene sequences at the genus level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial genera that averaged more than 1% of the relative abundance across all samples when sequencing <t>V3-V4</t> hypervariable regions are displayed. All other unassigned and classified OTUs belonged to genera comprising less than 1% of the total abundance represented as others/Unassigned
    V3 V4 Hypervariable Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 472 article reviews
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    91/100 stars
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    93
    Marine Biological Laboratory v4 v5 hypervariable regions
    Relative abundance of bacterial 16S rRNA gene sequences at the genus level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial genera that averaged more than 1% of the relative abundance across all samples when sequencing <t>V3-V4</t> hypervariable regions are displayed. All other unassigned and classified OTUs belonged to genera comprising less than 1% of the total abundance represented as others/Unassigned
    V4 V5 Hypervariable Regions, supplied by Marine Biological Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Illumina Inc v4 v5 hypervariable region
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    V4 V5 Hypervariable Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc pyrosequencingthe v3 v4 hypervariable region
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    Pyrosequencingthe V3 V4 Hypervariable Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher spectrophotometer hypervariable regions v3 v4
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    Spectrophotometer Hypervariable Regions V3 V4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs v4 hypervariable region materials phusion polymerase
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    V4 Hypervariable Region Materials Phusion Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc 16s rdna v3 v4 hypervariable regions
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    16s Rdna V3 V4 Hypervariable Regions, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rdna v3 v4 hypervariable regions/product/Illumina Inc
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    91
    Illumina Inc 16s rrna hypervariable v4 region
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    16s Rrna Hypervariable V4 Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna hypervariable v4 region/product/Illumina Inc
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    93
    Illumina Inc 16s ribosomal dna hypervariable regions v4
    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of <t>V4-V5</t> hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p
    16s Ribosomal Dna Hypervariable Regions V4, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Illumina Inc v3 v4 16s rrna hypervariable regions
    Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and <t>V3-V4</t> 16S <t>rRNA</t> gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA <t>hypervariable</t> region
    V3 V4 16s Rrna Hypervariable Regions, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v3 v4 16s rrna hypervariable regions/product/Illumina Inc
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    Illumina Inc 16s rrna gene hypervariable v4 region
    Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and <t>V3-V4</t> 16S <t>rRNA</t> gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA <t>hypervariable</t> region
    16s Rrna Gene Hypervariable V4 Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene hypervariable v4 region/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
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    16s rrna gene hypervariable v4 region - by Bioz Stars, 2020-08
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    88
    Illumina Inc 16s rrna gene v3 v4 hypervariable region
    Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and <t>V3-V4</t> 16S <t>rRNA</t> gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA <t>hypervariable</t> region
    16s Rrna Gene V3 V4 Hypervariable Region, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene v3 v4 hypervariable region/product/Illumina Inc
    Average 88 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene v3 v4 hypervariable region - by Bioz Stars, 2020-08
    88/100 stars
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    92
    Marine Biological Laboratory 16s rrna gene v4 v5 hypervariable region
    Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and <t>V3-V4</t> 16S <t>rRNA</t> gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA <t>hypervariable</t> region
    16s Rrna Gene V4 V5 Hypervariable Region, supplied by Marine Biological Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene v4 v5 hypervariable region/product/Marine Biological Laboratory
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene v4 v5 hypervariable region - by Bioz Stars, 2020-08
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    Image Search Results


    Pictorial representation of expected versus the actual relative abundance observed for the individual components of the mock bacterial community on the MiSeq and Roche454 GS Junior platform for primer pair targeting the ( a ) V1-V3 hypervariable region and ( b ) V3-V4 hypervariable region. Note: In the case of Pseudomonadaceae the MiSeq was able to identify the bacterial component at very low abundance

    Journal: BMC Microbiology

    Article Title: Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

    doi: 10.1186/s12866-017-0927-4

    Figure Lengend Snippet: Pictorial representation of expected versus the actual relative abundance observed for the individual components of the mock bacterial community on the MiSeq and Roche454 GS Junior platform for primer pair targeting the ( a ) V1-V3 hypervariable region and ( b ) V3-V4 hypervariable region. Note: In the case of Pseudomonadaceae the MiSeq was able to identify the bacterial component at very low abundance

    Article Snippet: The V4 hypervariable region is traditionally selected for work on the MiSeq as it provides adequate information for taxonomic classification of microbial communities and has demonstrated a lower error rate on the Illumina platform [ ].

    Techniques:

    Relative abundance of bacterial 16S rRNA gene sequences at the genus level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial genera that averaged more than 1% of the relative abundance across all samples when sequencing V3-V4 hypervariable regions are displayed. All other unassigned and classified OTUs belonged to genera comprising less than 1% of the total abundance represented as others/Unassigned

    Journal: BMC Microbiology

    Article Title: Relationship between nasopharyngeal and bronchoalveolar microbial communities in clinically healthy feedlot cattle

    doi: 10.1186/s12866-017-1042-2

    Figure Lengend Snippet: Relative abundance of bacterial 16S rRNA gene sequences at the genus level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial genera that averaged more than 1% of the relative abundance across all samples when sequencing V3-V4 hypervariable regions are displayed. All other unassigned and classified OTUs belonged to genera comprising less than 1% of the total abundance represented as others/Unassigned

    Article Snippet: Genomic DNA from each sample was extracted, and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and sequenced using Illumina Miseq platform.

    Techniques: Sequencing

    Relative abundance of bacterial 16S rRNA gene sequences at the phylum level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial phyla that averaged more than 1% of the relative abundance across all samples when sequencing V3-V4 hypervariable regions are displayed. All other unassigned and classified OTUs belonged to phyla comprising less than 1% of the total abundance represented as others/Unassigned

    Journal: BMC Microbiology

    Article Title: Relationship between nasopharyngeal and bronchoalveolar microbial communities in clinically healthy feedlot cattle

    doi: 10.1186/s12866-017-1042-2

    Figure Lengend Snippet: Relative abundance of bacterial 16S rRNA gene sequences at the phylum level observed in the NPS a and BAL b samples from 8 healthy feedlot calves. Only those bacterial phyla that averaged more than 1% of the relative abundance across all samples when sequencing V3-V4 hypervariable regions are displayed. All other unassigned and classified OTUs belonged to phyla comprising less than 1% of the total abundance represented as others/Unassigned

    Article Snippet: Genomic DNA from each sample was extracted, and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and sequenced using Illumina Miseq platform.

    Techniques: Sequencing

    Relative abundance of the genera found by the metagenomic approaches. Operational Taxonomic Units (OTUs) distribution in Odiel saline ponds (33% salinity) obtained from Illumina MiSeq sequencing of the 16S rRNA V3, V4 hypervariable regions. Four data sets were obtained from two different sequencing services. The graphic shows the percentage of the genera with more than 0.2% abundance. Minor genera include all the genera below 0.2%. The sequences that clustered together but could not been affiliated to a genus are named as “clusters” and have been divided in “Major clusters” ( > 0.2%) and “Minor clusters” (

    Journal: Marine Drugs

    Article Title: Characterization of the Microbial Population Inhabiting a Solar Saltern Pond of the Odiel Marshlands (SW Spain)

    doi: 10.3390/md16090332

    Figure Lengend Snippet: Relative abundance of the genera found by the metagenomic approaches. Operational Taxonomic Units (OTUs) distribution in Odiel saline ponds (33% salinity) obtained from Illumina MiSeq sequencing of the 16S rRNA V3, V4 hypervariable regions. Four data sets were obtained from two different sequencing services. The graphic shows the percentage of the genera with more than 0.2% abundance. Minor genera include all the genera below 0.2%. The sequences that clustered together but could not been affiliated to a genus are named as “clusters” and have been divided in “Major clusters” ( > 0.2%) and “Minor clusters” (

    Article Snippet: In both cases the PCR libraries were prepared by targeting the V3-V4 hypervariable regions of the 16S rRNA [ ] with the previously validated [ ] IlluAdp16S primers ( ) and sequenced using the Illumina MiSeq Reagent kits, V2× 250bp or V3× 300 bp, following Illumina recommendations for Library preparation and metagenomic sequencing.

    Techniques: Sequencing

    Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of V4-V5 hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p

    Journal: PLoS ONE

    Article Title: Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity

    doi: 10.1371/journal.pone.0134893

    Figure Lengend Snippet: Relative Operational Taxonomic Units (OTUs) abundance at the genus level in control (C), fructose-fed (F), and fructose-fed+faecal samples (FT) rats. Composition of caecal microbiota of rats from different diet groups as revealed by Illumina sequencing of V4-V5 hypervariable region of 16S rRNA gene. Population analyses for each diet group show phylotypes at genus level (when it was possible) and are reported as means of six rats for each diet group ( global R = 0.71 p

    Article Snippet: Composition of caecal microbiota of CA and FA rats as revealed by Illumina sequencing of V4-V5 hypervariable region of 16S rRNA gene.

    Techniques: Sequencing

    Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and V3-V4 16S rRNA gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA hypervariable region

    Journal: Parasites & Vectors

    Article Title: Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia

    doi: 10.1186/s13071-017-2579-5

    Figure Lengend Snippet: Molecular identification of reptile-associated Borrelia species in the Goanna tick ( Bothriocroton undatum ) from Sydney, Australia. a Summary table showing the V1-V3 and V3-V4 16S rRNA gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b , c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 ( b ) and V3-V4 ( c ) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA hypervariable region

    Article Snippet: Sequencing of the V1-V3 and V3-V4 16S rRNA hypervariable regions was performed on the Illumina Miseq (300-nt pair-end) using the following assay; 16S (V1-V3): 27F (5′-AGA GTT TGA TCM TGG CTC AG-3′) with 519R (5′-GWA TTA CCG CGG CKG CTG-3′) and 16S (V3-V4) 341F (5′-CCT AYG GGR BGC ASC AG-3′) with 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′).

    Techniques: Sequencing, Amplification

    Summary of a workflow taking advantage of the symmetrical body plan of ticks. All tick samples were longitudinally bisected, each half being subjected to DNA extraction by either the ISOLATE Fecal DNA kit (Method 1) or the ISOLATE II Genomic DNA Kit (Method 2). All samples were sequenced using gene diversity profiling assays, targeting both the V1-V3 and V3-V4 16S rRNA hypervariable regions

    Journal: Parasites & Vectors

    Article Title: Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia

    doi: 10.1186/s13071-017-2579-5

    Figure Lengend Snippet: Summary of a workflow taking advantage of the symmetrical body plan of ticks. All tick samples were longitudinally bisected, each half being subjected to DNA extraction by either the ISOLATE Fecal DNA kit (Method 1) or the ISOLATE II Genomic DNA Kit (Method 2). All samples were sequenced using gene diversity profiling assays, targeting both the V1-V3 and V3-V4 16S rRNA hypervariable regions

    Article Snippet: Sequencing of the V1-V3 and V3-V4 16S rRNA hypervariable regions was performed on the Illumina Miseq (300-nt pair-end) using the following assay; 16S (V1-V3): 27F (5′-AGA GTT TGA TCM TGG CTC AG-3′) with 519R (5′-GWA TTA CCG CGG CKG CTG-3′) and 16S (V3-V4) 341F (5′-CCT AYG GGR BGC ASC AG-3′) with 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′).

    Techniques: DNA Extraction

    Bacterial diversity profiles of the Goanna tick ( Bothriocroton undatum ). Bacterial abundance and bias at family, order, class and phylum levels were retrieved from a combination of different workflows that included V1-V3 or V3-V4 16S rRNA gene diversity profiles assays, and either Method 1 or Method 2 for the Goanna tick ( Bothriocroton undatum ). a Graphs with bacterial families and orders show only those with significant bias between the workflows. The graph depicting bacterial classes demonstrates the five most dominant bacterial classes found in each of the workflows, including spirochetes ( Borrelia spp.). The graph of bacterial phyla shows no bias found between any of the workflows. Bias between bacterial abundances at different taxonomical levels was evaluated using Tukey’s multiple comparisons test, ( P

    Journal: Parasites & Vectors

    Article Title: Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia

    doi: 10.1186/s13071-017-2579-5

    Figure Lengend Snippet: Bacterial diversity profiles of the Goanna tick ( Bothriocroton undatum ). Bacterial abundance and bias at family, order, class and phylum levels were retrieved from a combination of different workflows that included V1-V3 or V3-V4 16S rRNA gene diversity profiles assays, and either Method 1 or Method 2 for the Goanna tick ( Bothriocroton undatum ). a Graphs with bacterial families and orders show only those with significant bias between the workflows. The graph depicting bacterial classes demonstrates the five most dominant bacterial classes found in each of the workflows, including spirochetes ( Borrelia spp.). The graph of bacterial phyla shows no bias found between any of the workflows. Bias between bacterial abundances at different taxonomical levels was evaluated using Tukey’s multiple comparisons test, ( P

    Article Snippet: Sequencing of the V1-V3 and V3-V4 16S rRNA hypervariable regions was performed on the Illumina Miseq (300-nt pair-end) using the following assay; 16S (V1-V3): 27F (5′-AGA GTT TGA TCM TGG CTC AG-3′) with 519R (5′-GWA TTA CCG CGG CKG CTG-3′) and 16S (V3-V4) 341F (5′-CCT AYG GGR BGC ASC AG-3′) with 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′).

    Techniques: