Journal: Frontiers in Pharmacology
Article Title: Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells
Figure Lengend Snippet: Chronic esomeprazole does not disrupt epithelial integrity of CF hAECs. (A) Effect of increasing concentrations of apical esomeprazole on TransEpithelial Electrical Resistance (TEER) and Fluorescein Flux measured in Ussing chambers in the presence of a basolateral to apical Cl - gradient ( n = 5, five donors). (B,C) Effect of increasing concentrations of apical eso on resting short-circuit current (Isc, B , n = 5), and (C) amiloride-sensitive Isc (ΔIsc (amil) , black triangle, n = 5), Fsk-induced Isc (ΔIsc (Fsk) , open triangle, n = 5), CFTRinh172-sensitive Isc (ΔIsc (172) , open diamond, n = 5) and UTP-induced Isc (ΔIsc (UTP) , black circles, n = 5).
Article Snippet: Ouabain (O3125), esomeprazole (E7906), N -Acetyl- L -cysteine (A7250), amiloride (A7410), fluorescein (F6377), mitomycin C (M0503), and UTP (U6750) were purchased from Sigma-Aldrich.