Journal: The Journal of Neuroscience
Article Title: Network Architecture of Gap Junction-Coupled Neuronal Linkage in the Striatum
Figure Lengend Snippet: Identification of neuronal gap junctions in the striatum. A , Two PV-immunoreactive dendrites (d) make direct contact with each other. B , Enlargement of the contact site in A , demonstrating a typical ultrastructure of gap junction. C , Dual CLSM image showing Cx36 (red)-immunoreactive punctuate structure located at the contact site (arrow) between two PV (green)-immunoreactive dendrites (asterisks), one running horizontally while the other perpendicular to the image. D , The same contact site shown in C was re-examined in EM by converting fluorescent signals for PV to DAB reaction products. High-power view (inset) indicates strict correspondence of Cx36 labeling in CLSM to gap junction in EM. E–L , Functional and dynamic aspects of gap junctional coupling. After taking dual CLSM image (PV green; Cx36 red), the same contacting dendrites (d) were identified by EM ( G ), reconstructed from serial ultrathin sections ( F ), and confirmed to form gap junction between them ( H ). Blue dots on the surface of reconstructed dendrites in F indicate sites of synaptic contacts. Red arrowheads in G and H demarcate gap junction. Serial ultrathin sections ( I–L ) next to H demonstrate a presumed internalization of gap junction (blue arrows). Images in J and K are taken from the same specimen with different tilt angles for EM observations. Coated pits are visible around the internalized membranes in K . M , Enlargement of the framed area (bottom) in L . Note hexagonal array of spot-like reaction products. N , Enlargement of the framed area (top) in L , showing an alternating pattern of reaction products along the central line of the gap junction. Scale bars: A , C , G , 1 μm; B , 50 nm; D , 0.5 μm; inset, 100 nm; E , F , 10 μm; H-L , 100 nm; M , N , 10 nm.
Article Snippet: Serial ultrathin sections were collected in formvar-coated single slot grids, stained with uranyl acetate and lead citrate, and examined in a transmission electron microscope (H-7100, Hitachi).
Techniques: Confocal Laser Scanning Microscopy, Labeling, Functional Assay