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  • 95
    JEOL ultrathin sections
    Immunoelectron microscopic analysis showing GDNF-positive signals along the basal surface of Sertoli cells in hamsters. Electron micrographs showing transverse <t>ultrathin</t> sections of whole seminiferous tubules immunostained with anti-GDNF antibodies (DAB-osmium black). GDNF-positive signals are observed in the basal surface of Sertoli cells (S) adjacent to the spermatogonia (arrows) and the basal lamina (open arrowheads). Some signals are detected in the pinocytotic vesicles of peritubular myoid cells (double arrowhead in F). No signals are detected in the basal compartment of seminiferous tubules at stage XI (H, I). Panels B and C are higher magnified images of the basal surface of Sertoli cells in panel A. Asterisks, peritubular myoid cells; bl, basal lamina; G, spermatogenic cells; S, Sertoli cells. Scale bars represent 1 µm.
    Ultrathin Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 16308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Hitachi Ltd ultrathin sections
    Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. <t>Ultrathin</t> sections were probed with
    Ultrathin Sections, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 94/100, based on 7544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Hitachi ultrathin sections
    Electron micrographs (A, C), a semi-thin section (B), and an Epon block after the cutting of <t>ultrathin</t> sections (D) of osteopontin (OPN) immunoreactivities in the transplanted tooth at 3 days after operation. D, dentin; PC, pulp chamber; PD, predentin;
    Ultrathin Sections, supplied by Hitachi, used in various techniques. Bioz Stars score: 93/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare ultrathin sections
    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and <t>ultrathin</t> sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.
    Ultrathin Sections, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 6732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss ultrathin sections
    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) <t>Ultrathin</t> section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
    Ultrathin Sections, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 6314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Leica Microsystems ultrathin sections
    Ultrastructure of Mf. australis strain IT-1. Ultrastructure of Mf. australis strain IT-1. a Whole mount TEM image showing a single magnetosome chain, P-rich (P) and sulfur (S) granules; ( b ) <t>Ultrathin</t> section TEM image of high pressure frozen and freeze-substituted cells showing P-rich (P) and sulfur (S) granules, two magnetosomes ( black arrows ), a flagella bundle (F) associated with chemoreceptor array ( white arrows ), and a fibrillar layer at the cell surface ( arrowheads ). Uncharacterized globular structures (G) embedded in an electron-lucent material (asterisks) can be observed
    Ultrathin Sections, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 94/100, based on 4396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    FUJIFILM ultrathin endoscope
    The tip of the <t>ultrathin</t> endoscopes: (A) EG-580NW (Fujifilm Corp.) with a 2.0-mm working channel (arrow) and 5.9-mm distal end diameter, and (B) EG- 580NW2 with a 2.4-mm working channel (arrow) and 5.8-mm distal end diameter. The common specifications for the two endoscopes are as follows: field of view, 140o; flexible portion diameter, 5.9 mm; and total length, 1,400 mm.
    Ultrathin Endoscope, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Ted Pella ultrathin sections
    Ultrastructural analysis of NSC in acutely infected DRG. Acutely infected DRG were fixed and embedded for standard EM. <t>Ultrathin</t> sections were counterstained with lead citrate and uranyl acetate and investigated by TEM. The NSC shown in panels A to C
    Ultrathin Sections, supplied by Ted Pella, used in various techniques. Bioz Stars score: 93/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Ted Pella ultrathin carbon film
    Diameters of Mnx and MnxEF particles determined by TEM. Particles were deposited onto a 300 mesh gold TEM grid with <t>ultrathin</t> carbon film on lacey carbon support film and counterstained with nanoW. a Representative image of the Mnx complex. b Representative image of MnxEF particles. Scale bars are 10 nm. Size distribution of c Mnx and d MnxEF particles from TEM measurements. Histograms show the number of particles counted within a specific diameter range (
    Ultrathin Carbon Film, supplied by Ted Pella, used in various techniques. Bioz Stars score: 89/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Olympus ultrathin endoscope
    A case of <t>ultrathin</t> endoscope-assisted method. (A) The ultrathin endoscope approaching the orifice of the stenotic lesion is shown by fluoroscopy. (B) The guide wire insertion through the ultrathin endoscope is shown by fluoroscopy. (C) The ultrathin endoscope exchanged for the two channel endoscope is shown by fluoroscopy. (D) The balloon dilating the stenotic lesion is shown by fluoroscopy. (E) The orifice of the stenotic lesion is found at the duodenal bulb by ultrathin endoscope. (F) The guide wire is placed at the second portion of the duodenum after passing through the stenotic lesion by the ultrathin endoscope. (G) The guide wire is placed in the stenotic lesion by the two channel endoscope. (H) The balloon is dilating the stenotic lesion in a through the scope manner. (I) After dilatation, the orifice of the stenotic lesion is expanded.
    Ultrathin Endoscope, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    JEOL ultrathin 80 nm sections
    A case of <t>ultrathin</t> endoscope-assisted method. (A) The ultrathin endoscope approaching the orifice of the stenotic lesion is shown by fluoroscopy. (B) The guide wire insertion through the ultrathin endoscope is shown by fluoroscopy. (C) The ultrathin endoscope exchanged for the two channel endoscope is shown by fluoroscopy. (D) The balloon dilating the stenotic lesion is shown by fluoroscopy. (E) The orifice of the stenotic lesion is found at the duodenal bulb by ultrathin endoscope. (F) The guide wire is placed at the second portion of the duodenum after passing through the stenotic lesion by the ultrathin endoscope. (G) The guide wire is placed in the stenotic lesion by the two channel endoscope. (H) The balloon is dilating the stenotic lesion in a through the scope manner. (I) After dilatation, the orifice of the stenotic lesion is expanded.
    Ultrathin 80 Nm Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 89/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA ultrathin sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Sections, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ImmunoReagents ultrathin sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Sections, supplied by ImmunoReagents, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    JEOL ultrathin 70 nm sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin 70 Nm Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 90/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Olympus ultrathin bronchoscope
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Bronchoscope, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    JEOL ultrathin 60 nm sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin 60 Nm Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 91/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    NISSHIN ultrathin sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Sections, supplied by NISSHIN, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co ultrathin sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Sections, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunoelectron microscopic analysis showing GDNF-positive signals along the basal surface of Sertoli cells in hamsters. Electron micrographs showing transverse ultrathin sections of whole seminiferous tubules immunostained with anti-GDNF antibodies (DAB-osmium black). GDNF-positive signals are observed in the basal surface of Sertoli cells (S) adjacent to the spermatogonia (arrows) and the basal lamina (open arrowheads). Some signals are detected in the pinocytotic vesicles of peritubular myoid cells (double arrowhead in F). No signals are detected in the basal compartment of seminiferous tubules at stage XI (H, I). Panels B and C are higher magnified images of the basal surface of Sertoli cells in panel A. Asterisks, peritubular myoid cells; bl, basal lamina; G, spermatogenic cells; S, Sertoli cells. Scale bars represent 1 µm.

    Journal: PLoS ONE

    Article Title: Cyclical and Patch-Like GDNF Distribution along the Basal Surface of Sertoli Cells in Mouse and Hamster Testes

    doi: 10.1371/journal.pone.0028367

    Figure Lengend Snippet: Immunoelectron microscopic analysis showing GDNF-positive signals along the basal surface of Sertoli cells in hamsters. Electron micrographs showing transverse ultrathin sections of whole seminiferous tubules immunostained with anti-GDNF antibodies (DAB-osmium black). GDNF-positive signals are observed in the basal surface of Sertoli cells (S) adjacent to the spermatogonia (arrows) and the basal lamina (open arrowheads). Some signals are detected in the pinocytotic vesicles of peritubular myoid cells (double arrowhead in F). No signals are detected in the basal compartment of seminiferous tubules at stage XI (H, I). Panels B and C are higher magnified images of the basal surface of Sertoli cells in panel A. Asterisks, peritubular myoid cells; bl, basal lamina; G, spermatogenic cells; S, Sertoli cells. Scale bars represent 1 µm.

    Article Snippet: Ultrathin sections were cut, and then observed under a JEM 1010 transmission electron microscope (JEOL, Japan) at 80 kV.

    Techniques:

    TPPPS interferes with viral adsorption to DF-1 cells. DF-1 monolayers were infected with 10 4.75 TCID 50 of ALV-J in the absence ( A ) or presence ( B ) of TPPPS treatment (50 μg/mL). At 4 h post-infection, the cells were centrifuged and fixed to prepare ultrathin sections, and the virions in cells were imaged via TEM (30000×). The virions on the cell membrane surface were denoted by black circles and those in the endosomes were denoted by black arrowheads. The purified ALV-J ( C ) and the mixture of ALV-J and TPPPS ( D ) were imaged via TEM after negative staining (60000×). The virions were denoted by black arrowheads.

    Journal: Scientific Reports

    Article Title: Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

    doi: 10.1038/srep44353

    Figure Lengend Snippet: TPPPS interferes with viral adsorption to DF-1 cells. DF-1 monolayers were infected with 10 4.75 TCID 50 of ALV-J in the absence ( A ) or presence ( B ) of TPPPS treatment (50 μg/mL). At 4 h post-infection, the cells were centrifuged and fixed to prepare ultrathin sections, and the virions in cells were imaged via TEM (30000×). The virions on the cell membrane surface were denoted by black circles and those in the endosomes were denoted by black arrowheads. The purified ALV-J ( C ) and the mixture of ALV-J and TPPPS ( D ) were imaged via TEM after negative staining (60000×). The virions were denoted by black arrowheads.

    Article Snippet: Ultrathin (50 nm, LKB-V) longitudinal sections were prepared after the location in semi-thin section, stained with uranyl acetate and lead citrate, and then examined under a JEOL-1200EX electron microscope (JEOL, Japan).

    Techniques: Adsorption, Infection, Transmission Electron Microscopy, Purification, Negative Staining

    Cx36 is localized both in cytoplasmic vesicular structures and gap junctions. A, B , Laser-scanning images of cytoplasmic Cx36 immunofluorescence localized in large somata in the GCL. Intracellular immunoreactivity (red) is localized in vesicular structures within large somata in the GCL. In B , the immunoreactivity (red) was merged in a bright-field image of the GCL. Note that localization of cytoplasmic immunofluorescence is restricted to the perinuclear region. Perikaryal immunoreactivity corresponds to the vesicles (arrows) shown in A. C , Immunoelectron micrograph demonstrating subcellular distribution of perikaryal vesicular immunoreactivity within a large soma in the GCL, observed at 1000 kV by high-voltage electron microscopy of a 5-μm-thick section. Cytoplasmic immunoreactivity is, in particular, associated with a vesicular structure (arrow) in the perinuclear region. Nu, Cell nucleus. D , Low-power magnification by high-voltage immunoelectron microscopy of the IPL shows representative areas of immunopositive junctional contacts (arrows). E , Immunoelectron micrograph demonstrating gap junction plaques formed at sites of intercellular contact in the IPL of an ultrathin section. Plasma membranes of the two processes are closely apposed with a narrow central gap, 2.7 nm wide, between the outer leaflets of the apposed unit membranes. Scale bars: A, B , 20 μm; C, D , 2 μm; E , 100 nm.

    Journal: The Journal of Neuroscience

    Article Title: Dendrodendritic Electrical Synapses between Mammalian Retinal Ganglion Cells

    doi: 10.1523/JNEUROSCI.3319-04.2004

    Figure Lengend Snippet: Cx36 is localized both in cytoplasmic vesicular structures and gap junctions. A, B , Laser-scanning images of cytoplasmic Cx36 immunofluorescence localized in large somata in the GCL. Intracellular immunoreactivity (red) is localized in vesicular structures within large somata in the GCL. In B , the immunoreactivity (red) was merged in a bright-field image of the GCL. Note that localization of cytoplasmic immunofluorescence is restricted to the perinuclear region. Perikaryal immunoreactivity corresponds to the vesicles (arrows) shown in A. C , Immunoelectron micrograph demonstrating subcellular distribution of perikaryal vesicular immunoreactivity within a large soma in the GCL, observed at 1000 kV by high-voltage electron microscopy of a 5-μm-thick section. Cytoplasmic immunoreactivity is, in particular, associated with a vesicular structure (arrow) in the perinuclear region. Nu, Cell nucleus. D , Low-power magnification by high-voltage immunoelectron microscopy of the IPL shows representative areas of immunopositive junctional contacts (arrows). E , Immunoelectron micrograph demonstrating gap junction plaques formed at sites of intercellular contact in the IPL of an ultrathin section. Plasma membranes of the two processes are closely apposed with a narrow central gap, 2.7 nm wide, between the outer leaflets of the apposed unit membranes. Scale bars: A, B , 20 μm; C, D , 2 μm; E , 100 nm.

    Article Snippet: For analysis of junctional structures between the interconnected dendrites of GCs, serial ultrathin sections from the specimens were studied in a JEOL 1010, JEOL 1200EX, or Hitachi H-7000 conventional electron microscope at 75 or 80 kV fitted with a goniometer stage.

    Techniques: Immunofluorescence, Electron Microscopy, Immuno-Electron Microscopy

    Electron micrographs showing the dendrodendritic gap junctions between Neurobiotin-coupled neighboring α-GCs, revealed by high-voltage ( A, B ) and conventional ( C ) electron microscopy. A , The direct junctional contact (arrows) occurs between the tips of peripheral dendrites from coupled inner-type α-GCs. The specimen was observed at 1000 kV in a tangential 5-μm-thick section. B , Labeled dendrites make another direct contact with each other at dendritic tips in a tip-to-shank manner. Note the close apposition of the labeled dendrites of the two contacting cells as demarcated by arrows. C , High-magnification view of a gap junction formed between two labeled peripheral dendrites from coupled cells by conventional electron microscopy of an ultrathin section at 75 kV. Plasma membranes of the two labeled dendrites are closely apposed with a narrow central gap, 2.7 nm wide, between the outer leaflets of the apposed unit membranes. Scale bars: A, B , 10 μm; C , 200 nm.

    Journal: The Journal of Neuroscience

    Article Title: Dendrodendritic Electrical Synapses between Mammalian Retinal Ganglion Cells

    doi: 10.1523/JNEUROSCI.3319-04.2004

    Figure Lengend Snippet: Electron micrographs showing the dendrodendritic gap junctions between Neurobiotin-coupled neighboring α-GCs, revealed by high-voltage ( A, B ) and conventional ( C ) electron microscopy. A , The direct junctional contact (arrows) occurs between the tips of peripheral dendrites from coupled inner-type α-GCs. The specimen was observed at 1000 kV in a tangential 5-μm-thick section. B , Labeled dendrites make another direct contact with each other at dendritic tips in a tip-to-shank manner. Note the close apposition of the labeled dendrites of the two contacting cells as demarcated by arrows. C , High-magnification view of a gap junction formed between two labeled peripheral dendrites from coupled cells by conventional electron microscopy of an ultrathin section at 75 kV. Plasma membranes of the two labeled dendrites are closely apposed with a narrow central gap, 2.7 nm wide, between the outer leaflets of the apposed unit membranes. Scale bars: A, B , 10 μm; C , 200 nm.

    Article Snippet: For analysis of junctional structures between the interconnected dendrites of GCs, serial ultrathin sections from the specimens were studied in a JEOL 1010, JEOL 1200EX, or Hitachi H-7000 conventional electron microscope at 75 or 80 kV fitted with a goniometer stage.

    Techniques: Electron Microscopy, Labeling

    Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. Ultrathin sections were probed with

    Journal: Journal of Virology

    Article Title: Vaccinia Virus Mutations in the L4R Gene Encoding a Virion Structural Protein Produce Abnormal Mature Particles Lacking a Nucleocapsid

    doi: 10.1128/JVI.02126-14

    Figure Lengend Snippet: Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. Ultrathin sections were probed with

    Article Snippet: Ultrathin sections (70 to 80 nm) were poststained with 2% uranyl acetate and lead citrate and examined with an H-7000 transmission electron microscope (TEM; Hitachi High Technologies America, Inc., Schaumburg, IL) operated at 100 kV.

    Techniques: Labeling, Infection, Incubation, Immuno-Electron Microscopy

    Identification of neuronal gap junctions in the striatum. A , Two PV-immunoreactive dendrites (d) make direct contact with each other. B , Enlargement of the contact site in A , demonstrating a typical ultrastructure of gap junction. C , Dual CLSM image showing Cx36 (red)-immunoreactive punctuate structure located at the contact site (arrow) between two PV (green)-immunoreactive dendrites (asterisks), one running horizontally while the other perpendicular to the image. D , The same contact site shown in C was re-examined in EM by converting fluorescent signals for PV to DAB reaction products. High-power view (inset) indicates strict correspondence of Cx36 labeling in CLSM to gap junction in EM. E–L , Functional and dynamic aspects of gap junctional coupling. After taking dual CLSM image (PV green; Cx36 red), the same contacting dendrites (d) were identified by EM ( G ), reconstructed from serial ultrathin sections ( F ), and confirmed to form gap junction between them ( H ). Blue dots on the surface of reconstructed dendrites in F indicate sites of synaptic contacts. Red arrowheads in G and H demarcate gap junction. Serial ultrathin sections ( I–L ) next to H demonstrate a presumed internalization of gap junction (blue arrows). Images in J and K are taken from the same specimen with different tilt angles for EM observations. Coated pits are visible around the internalized membranes in K . M , Enlargement of the framed area (bottom) in L . Note hexagonal array of spot-like reaction products. N , Enlargement of the framed area (top) in L , showing an alternating pattern of reaction products along the central line of the gap junction. Scale bars: A , C , G , 1 μm; B , 50 nm; D , 0.5 μm; inset, 100 nm; E , F , 10 μm; H-L , 100 nm; M , N , 10 nm.

    Journal: The Journal of Neuroscience

    Article Title: Network Architecture of Gap Junction-Coupled Neuronal Linkage in the Striatum

    doi: 10.1523/JNEUROSCI.4418-08.2009

    Figure Lengend Snippet: Identification of neuronal gap junctions in the striatum. A , Two PV-immunoreactive dendrites (d) make direct contact with each other. B , Enlargement of the contact site in A , demonstrating a typical ultrastructure of gap junction. C , Dual CLSM image showing Cx36 (red)-immunoreactive punctuate structure located at the contact site (arrow) between two PV (green)-immunoreactive dendrites (asterisks), one running horizontally while the other perpendicular to the image. D , The same contact site shown in C was re-examined in EM by converting fluorescent signals for PV to DAB reaction products. High-power view (inset) indicates strict correspondence of Cx36 labeling in CLSM to gap junction in EM. E–L , Functional and dynamic aspects of gap junctional coupling. After taking dual CLSM image (PV green; Cx36 red), the same contacting dendrites (d) were identified by EM ( G ), reconstructed from serial ultrathin sections ( F ), and confirmed to form gap junction between them ( H ). Blue dots on the surface of reconstructed dendrites in F indicate sites of synaptic contacts. Red arrowheads in G and H demarcate gap junction. Serial ultrathin sections ( I–L ) next to H demonstrate a presumed internalization of gap junction (blue arrows). Images in J and K are taken from the same specimen with different tilt angles for EM observations. Coated pits are visible around the internalized membranes in K . M , Enlargement of the framed area (bottom) in L . Note hexagonal array of spot-like reaction products. N , Enlargement of the framed area (top) in L , showing an alternating pattern of reaction products along the central line of the gap junction. Scale bars: A , C , G , 1 μm; B , 50 nm; D , 0.5 μm; inset, 100 nm; E , F , 10 μm; H-L , 100 nm; M , N , 10 nm.

    Article Snippet: Serial ultrathin sections were collected in formvar-coated single slot grids, stained with uranyl acetate and lead citrate, and examined in a transmission electron microscope (H-7100, Hitachi).

    Techniques: Confocal Laser Scanning Microscopy, Labeling, Functional Assay

    Electron micrographs (A, C), a semi-thin section (B), and an Epon block after the cutting of ultrathin sections (D) of osteopontin (OPN) immunoreactivities in the transplanted tooth at 3 days after operation. D, dentin; PC, pulp chamber; PD, predentin;

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: The Expression of GM-CSF and Osteopontin in Immunocompetent Cells Precedes the Odontoblast Differentiation Following Allogenic Tooth Transplantation in Mice

    doi: 10.1369/0022155411403314

    Figure Lengend Snippet: Electron micrographs (A, C), a semi-thin section (B), and an Epon block after the cutting of ultrathin sections (D) of osteopontin (OPN) immunoreactivities in the transplanted tooth at 3 days after operation. D, dentin; PC, pulp chamber; PD, predentin;

    Article Snippet: Ultrathin sections (70 nm thick) were double-stained with uranyl acetate and lead citrate and examined with an H-7100 transmission electron microscope (Hitachi High-Technologies Corp., Tokyo, Japan).

    Techniques: Blocking Assay

    Electron micrographs (A, C) and a semithin section (B), as well as an Epon block after the cutting of ultrathin sections (D) of granulocyte macrophage colony-stimulating factor (GM-CSF) immunoreactivity in the transplanted tooth at 3 days after operation.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: The Expression of GM-CSF and Osteopontin in Immunocompetent Cells Precedes the Odontoblast Differentiation Following Allogenic Tooth Transplantation in Mice

    doi: 10.1369/0022155411403314

    Figure Lengend Snippet: Electron micrographs (A, C) and a semithin section (B), as well as an Epon block after the cutting of ultrathin sections (D) of granulocyte macrophage colony-stimulating factor (GM-CSF) immunoreactivity in the transplanted tooth at 3 days after operation.

    Article Snippet: Ultrathin sections (70 nm thick) were double-stained with uranyl acetate and lead citrate and examined with an H-7100 transmission electron microscope (Hitachi High-Technologies Corp., Tokyo, Japan).

    Techniques: Blocking Assay

    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Journal: Journal of Virology

    Article Title: Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber

    doi:

    Figure Lengend Snippet: Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Article Snippet: Ultrathin sections were observed under a Philips CM120 Biotwin electron microscope at 120 kV.

    Techniques: Electron Microscopy, Infection, Produced, Staining

    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Electron micrographs of PKCα immunoreactivity in the outer plexiform layer of a 5-week-old Rho −/− mouse retina. Synaptic ribbons are marked by arrowheads and horizontal cell processes by “h.” A , Rod spherule (rs) with a presynaptic ribbon, two lateral horizontal cell processes, and a PKCα-labeled invaginating rod bipolar cell dendrite. B , C , Section numbers 1 and 6 of a series of ultrathin sections showing a cone pedicle (cp) with a heavily stained dendrite making flat contact in B (arrow) and invaginating contact in C (asterisk). D , E , Two additional examples of PKCα-immunoreactive dendritic tips making invaginating synaptic contact with the cone pedicle. Scale bar: (in D ) 1 μm.

    Journal: The Journal of Neuroscience

    Article Title: Synaptic Plasticity in CNGA3−/− Mice: Cone Bipolar Cells React on the Missing Cone Input and Form Ectopic Synapses with Rods

    doi: 10.1523/JNEUROSCI.4483-05.2006

    Figure Lengend Snippet: Electron micrographs of PKCα immunoreactivity in the outer plexiform layer of a 5-week-old Rho −/− mouse retina. Synaptic ribbons are marked by arrowheads and horizontal cell processes by “h.” A , Rod spherule (rs) with a presynaptic ribbon, two lateral horizontal cell processes, and a PKCα-labeled invaginating rod bipolar cell dendrite. B , C , Section numbers 1 and 6 of a series of ultrathin sections showing a cone pedicle (cp) with a heavily stained dendrite making flat contact in B (arrow) and invaginating contact in C (asterisk). D , E , Two additional examples of PKCα-immunoreactive dendritic tips making invaginating synaptic contact with the cone pedicle. Scale bar: (in D ) 1 μm.

    Article Snippet: Serial ultrathin sections were cut, stained with uranyl acetate and lead citrate, and examined with a Zeiss EM10 electron microscope.

    Techniques: Labeling, Staining

    Ultrastructure of Mf. australis strain IT-1. Ultrastructure of Mf. australis strain IT-1. a Whole mount TEM image showing a single magnetosome chain, P-rich (P) and sulfur (S) granules; ( b ) Ultrathin section TEM image of high pressure frozen and freeze-substituted cells showing P-rich (P) and sulfur (S) granules, two magnetosomes ( black arrows ), a flagella bundle (F) associated with chemoreceptor array ( white arrows ), and a fibrillar layer at the cell surface ( arrowheads ). Uncharacterized globular structures (G) embedded in an electron-lucent material (asterisks) can be observed

    Journal: BMC Genomics

    Article Title: Combined genomic and structural analyses of a cultured magnetotactic bacterium reveals its niche adaptation to a dynamic environment

    doi: 10.1186/s12864-016-3064-9

    Figure Lengend Snippet: Ultrastructure of Mf. australis strain IT-1. Ultrastructure of Mf. australis strain IT-1. a Whole mount TEM image showing a single magnetosome chain, P-rich (P) and sulfur (S) granules; ( b ) Ultrathin section TEM image of high pressure frozen and freeze-substituted cells showing P-rich (P) and sulfur (S) granules, two magnetosomes ( black arrows ), a flagella bundle (F) associated with chemoreceptor array ( white arrows ), and a fibrillar layer at the cell surface ( arrowheads ). Uncharacterized globular structures (G) embedded in an electron-lucent material (asterisks) can be observed

    Article Snippet: Ultrathin sections were obtained on Leica EM U6 ultramicrotome (Leica Microsystems, Bannockburn, IL, USA), stained with uranyl acetate and lead citrate and imaged with a Morgagni transmission electron microscope (FEI Company, Hillsboro, OR, USA) at 80kV.

    Techniques: Transmission Electron Microscopy

    WSN-infected A549 cells recruit rough ER cisternae and a new type of organelle around the MTOC. a , b Immunofluorescence and confocal microscopy images (lateral and frontal merge, respectively) of A549 cells expressing HA-tagged Rab11 and infected with the WSN virus (8 hpi). Rab11 (red) and viral NP (green) co-exist in discreet spots near the nucleus (arrows). ( c ) Ultrathin-section of a mock-infected A549 cell as visualized by transmission electron microscopy (TEM). The MTOC area near the nucleus is shown. Mitochondria (mi) surround the centrioles ( c ) near the nucleus (N). d Equivalent MTOC region in a WSN-infected A549 cell at 8 hpi showing swollen rough ER cisternae (arrowheads) and numerous irregularly coated vesicles (ICVs, arrows) that are a new type of organelle. e Close-up of a pair of ICVs. f General view of perinuclear region in a WSN-infected A549 cell at 8 hpi showing modified rough ER (black arrowheads), groups of ribosomes (white arrowheads), and an ICV (arrow). Scale bars, 10 μm a , b ; 0.5 μm c , d , f ; 100 nm e

    Journal: Nature Communications

    Article Title: Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

    doi: 10.1038/s41467-017-01557-6

    Figure Lengend Snippet: WSN-infected A549 cells recruit rough ER cisternae and a new type of organelle around the MTOC. a , b Immunofluorescence and confocal microscopy images (lateral and frontal merge, respectively) of A549 cells expressing HA-tagged Rab11 and infected with the WSN virus (8 hpi). Rab11 (red) and viral NP (green) co-exist in discreet spots near the nucleus (arrows). ( c ) Ultrathin-section of a mock-infected A549 cell as visualized by transmission electron microscopy (TEM). The MTOC area near the nucleus is shown. Mitochondria (mi) surround the centrioles ( c ) near the nucleus (N). d Equivalent MTOC region in a WSN-infected A549 cell at 8 hpi showing swollen rough ER cisternae (arrowheads) and numerous irregularly coated vesicles (ICVs, arrows) that are a new type of organelle. e Close-up of a pair of ICVs. f General view of perinuclear region in a WSN-infected A549 cell at 8 hpi showing modified rough ER (black arrowheads), groups of ribosomes (white arrowheads), and an ICV (arrow). Scale bars, 10 μm a , b ; 0.5 μm c , d , f ; 100 nm e

    Article Snippet: Ultrathin (~70–80 nm) oriented serial sections were obtained with a UC6 ultramicrotome (Leica Microsystems), collected on uncoated 300-mesh copper grids (TAAB Laboratories), stained with saturated uranylacetate and lead citrate, and imaged by TEM.

    Techniques: Infection, Immunofluorescence, Confocal Microscopy, Expressing, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Modification

    The tip of the ultrathin endoscopes: (A) EG-580NW (Fujifilm Corp.) with a 2.0-mm working channel (arrow) and 5.9-mm distal end diameter, and (B) EG- 580NW2 with a 2.4-mm working channel (arrow) and 5.8-mm distal end diameter. The common specifications for the two endoscopes are as follows: field of view, 140o; flexible portion diameter, 5.9 mm; and total length, 1,400 mm.

    Journal: Clinical Endoscopy

    Article Title: An Ultrathin Endoscope with a 2.4-mm Working Channel Shortens the Esophagogastroduodenoscopy Time by Shortening the Suction Time

    doi: 10.5946/ce.2015.48.6.516

    Figure Lengend Snippet: The tip of the ultrathin endoscopes: (A) EG-580NW (Fujifilm Corp.) with a 2.0-mm working channel (arrow) and 5.9-mm distal end diameter, and (B) EG- 580NW2 with a 2.4-mm working channel (arrow) and 5.8-mm distal end diameter. The common specifications for the two endoscopes are as follows: field of view, 140o; flexible portion diameter, 5.9 mm; and total length, 1,400 mm.

    Article Snippet: Specifications of the ultrathin endoscopes We used a new ultrathin endoscope with a 2.4-mm working channel (EG-580NW2), and the existing ultrathin endoscope with a 2.0-mm working channel (EG-580NW) as the control ( ).

    Techniques:

    Ultrastructural analysis of NSC in acutely infected DRG. Acutely infected DRG were fixed and embedded for standard EM. Ultrathin sections were counterstained with lead citrate and uranyl acetate and investigated by TEM. The NSC shown in panels A to C

    Journal: Journal of Virology

    Article Title: Mechanisms of Varicella-Zoster Virus Neuropathogenesis in Human Dorsal Root Ganglia

    doi: 10.1128/JVI.02592-07

    Figure Lengend Snippet: Ultrastructural analysis of NSC in acutely infected DRG. Acutely infected DRG were fixed and embedded for standard EM. Ultrathin sections were counterstained with lead citrate and uranyl acetate and investigated by TEM. The NSC shown in panels A to C

    Article Snippet: Ultrathin sections were transferred to locator grids (Maxtaform, 200 mesh, nickel; Ted Pella).

    Techniques: Infection, Transmission Electron Microscopy

    Multiscale imaging of acutely infected DRG by correlative IF-EM. Ultrathin cryosections of acutely infected DRG were stained for IE63 (red) and collagen (green) (A to D). The primary antibody (anti-IE63) was detected with Texas red-labeled goat anti-rabbit

    Journal: Journal of Virology

    Article Title: Mechanisms of Varicella-Zoster Virus Neuropathogenesis in Human Dorsal Root Ganglia

    doi: 10.1128/JVI.02592-07

    Figure Lengend Snippet: Multiscale imaging of acutely infected DRG by correlative IF-EM. Ultrathin cryosections of acutely infected DRG were stained for IE63 (red) and collagen (green) (A to D). The primary antibody (anti-IE63) was detected with Texas red-labeled goat anti-rabbit

    Article Snippet: Ultrathin sections were transferred to locator grids (Maxtaform, 200 mesh, nickel; Ted Pella).

    Techniques: Imaging, Infection, Staining, Labeling

    Diameters of Mnx and MnxEF particles determined by TEM. Particles were deposited onto a 300 mesh gold TEM grid with ultrathin carbon film on lacey carbon support film and counterstained with nanoW. a Representative image of the Mnx complex. b Representative image of MnxEF particles. Scale bars are 10 nm. Size distribution of c Mnx and d MnxEF particles from TEM measurements. Histograms show the number of particles counted within a specific diameter range (

    Journal: Nature Communications

    Article Title: Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx

    doi: 10.1038/s41467-017-00896-8

    Figure Lengend Snippet: Diameters of Mnx and MnxEF particles determined by TEM. Particles were deposited onto a 300 mesh gold TEM grid with ultrathin carbon film on lacey carbon support film and counterstained with nanoW. a Representative image of the Mnx complex. b Representative image of MnxEF particles. Scale bars are 10 nm. Size distribution of c Mnx and d MnxEF particles from TEM measurements. Histograms show the number of particles counted within a specific diameter range (

    Article Snippet: In total, 5 μL of each reaction suspension was pipetted onto a 300 mesh gold TEM grid with ultrathin carbon film on lacey carbon support film (Ted Pella, Inc.).

    Techniques: Transmission Electron Microscopy

    A case of ultrathin endoscope-assisted method. (A) The ultrathin endoscope approaching the orifice of the stenotic lesion is shown by fluoroscopy. (B) The guide wire insertion through the ultrathin endoscope is shown by fluoroscopy. (C) The ultrathin endoscope exchanged for the two channel endoscope is shown by fluoroscopy. (D) The balloon dilating the stenotic lesion is shown by fluoroscopy. (E) The orifice of the stenotic lesion is found at the duodenal bulb by ultrathin endoscope. (F) The guide wire is placed at the second portion of the duodenum after passing through the stenotic lesion by the ultrathin endoscope. (G) The guide wire is placed in the stenotic lesion by the two channel endoscope. (H) The balloon is dilating the stenotic lesion in a through the scope manner. (I) After dilatation, the orifice of the stenotic lesion is expanded.

    Journal: Clinical Endoscopy

    Article Title: Ultrathin Endoscope-Assisted Method for the Management of Upper Gastrointestinal Obstruction to Avoid Technical Failure

    doi: 10.5946/ce.2013.46.4.373

    Figure Lengend Snippet: A case of ultrathin endoscope-assisted method. (A) The ultrathin endoscope approaching the orifice of the stenotic lesion is shown by fluoroscopy. (B) The guide wire insertion through the ultrathin endoscope is shown by fluoroscopy. (C) The ultrathin endoscope exchanged for the two channel endoscope is shown by fluoroscopy. (D) The balloon dilating the stenotic lesion is shown by fluoroscopy. (E) The orifice of the stenotic lesion is found at the duodenal bulb by ultrathin endoscope. (F) The guide wire is placed at the second portion of the duodenum after passing through the stenotic lesion by the ultrathin endoscope. (G) The guide wire is placed in the stenotic lesion by the two channel endoscope. (H) The balloon is dilating the stenotic lesion in a through the scope manner. (I) After dilatation, the orifice of the stenotic lesion is expanded.

    Article Snippet: The ultrathin endoscope could pass through the stenosis more easily than a conventional endoscope, thus making it possible to traverse the guide wire.

    Techniques:

    Schema of the ultrathin endoscope-assisted method. (A) The stenotic lesion was approached with a conventional scope. (B) When guide wire insertion failed with the conventional scope, the ultrathin endoscope passed through the stenotic lesion and the guide wire was inserted into the forceps channel. (C) The guide wire was placed in the stenotic lesion and the ultrathin endoscope was retrieved completely. (D) The tip of the guide wire was grabbed with an alligator forceps which was already inserted into the large bore channel of the two channel endoscope. (E) The guide wire was retrieved through the large bore channel of the two channel endoscope. (F) The two channel endoscope was inserted into the patient and approached to the stenotic lesion. (G) The self-expandable metallic stent was inserted and expanded in the stenotic lesion.

    Journal: Clinical Endoscopy

    Article Title: Ultrathin Endoscope-Assisted Method for the Management of Upper Gastrointestinal Obstruction to Avoid Technical Failure

    doi: 10.5946/ce.2013.46.4.373

    Figure Lengend Snippet: Schema of the ultrathin endoscope-assisted method. (A) The stenotic lesion was approached with a conventional scope. (B) When guide wire insertion failed with the conventional scope, the ultrathin endoscope passed through the stenotic lesion and the guide wire was inserted into the forceps channel. (C) The guide wire was placed in the stenotic lesion and the ultrathin endoscope was retrieved completely. (D) The tip of the guide wire was grabbed with an alligator forceps which was already inserted into the large bore channel of the two channel endoscope. (E) The guide wire was retrieved through the large bore channel of the two channel endoscope. (F) The two channel endoscope was inserted into the patient and approached to the stenotic lesion. (G) The self-expandable metallic stent was inserted and expanded in the stenotic lesion.

    Article Snippet: The ultrathin endoscope could pass through the stenosis more easily than a conventional endoscope, thus making it possible to traverse the guide wire.

    Techniques:

    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable

    Journal: Archives of Dermatological Research

    Article Title: Microscopic and ultrastructural evidences in human skin following calcium hydroxylapatite filler treatment

    doi: 10.1007/s00403-017-1734-3

    Figure Lengend Snippet: Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable

    Article Snippet: Ultrathin sections, after the collection on 200 mesh grids, were counterstained with lead citrate and uranyl acetate (Merck, Darmstadt, Germany).

    Techniques: Electron Microscopy