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  • 99
    New England Biolabs ultra directional rna seq kit
    Ultra Directional Rna Seq Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra directional rna seq kit/product/New England Biolabs
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    ultra directional rna seq kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    TaKaRa smart seq v4 ultra low rna seq kit
    Comparison of FL-cDNA-Seq and Illumina <t>RNA-Seq.</t> (A) The gene expression of FL-cDNA-Seq of LC2/ad (R9.4) was compared with that of TruSeq RNA (left) and <t>SMART-Seq</t> (right). Pearson correlation coefficients are shown on the graph. (B) Influence of sequencing depth on the estimation of gene expression level and gene detection. Reads for each method were randomly sampled in triplicate. The average of the Pearson correlation coefficients between TruSeq RNA and randomly sampled data for FL-cDNA-Seq and SMART-Seq is shown (left). The average number of genes with an expression level of more than 1 tpm or ppm is shown (right). (C) Comparison to qRT-qPCR. Forty-four genes detected by all methods were analyzed. The gene expression of these genes was normalized to GAPDH. Pearson correlation coefficients are shown on the graph. qRT-qPCR data of LC2/ad was obtained as in our previous study. 16
    Smart Seq V4 Ultra Low Rna Seq Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart seq v4 ultra low rna seq kit/product/TaKaRa
    Average 99 stars, based on 307 article reviews
    Price from $9.99 to $1999.99
    smart seq v4 ultra low rna seq kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    rna  (TaKaRa)
    94
    TaKaRa rna
    While most protein coding genes are commonly detected, lincRNAs appear more method specific. ( A ) Overlap between protein coding genes detected in <t>polyA[+]</t> (1 million reads) and total <t>RNA</t> (1 million reads) libraries. ( B ) Expression counts for protein coding genes detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray). ( C ) Overlap between lncRNAs detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( D ) Expression counts for lncRNAs detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray).
    Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 14186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/TaKaRa
    Average 94 stars, based on 14186 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    TaKaRa smart seq v4 ultra low input rna
    While most protein coding genes are commonly detected, lincRNAs appear more method specific. ( A ) Overlap between protein coding genes detected in <t>polyA[+]</t> (1 million reads) and total <t>RNA</t> (1 million reads) libraries. ( B ) Expression counts for protein coding genes detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray). ( C ) Overlap between lncRNAs detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( D ) Expression counts for lncRNAs detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray).
    Smart Seq V4 Ultra Low Input Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart seq v4 ultra low input rna/product/TaKaRa
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    smart seq v4 ultra low input rna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of FL-cDNA-Seq and Illumina RNA-Seq. (A) The gene expression of FL-cDNA-Seq of LC2/ad (R9.4) was compared with that of TruSeq RNA (left) and SMART-Seq (right). Pearson correlation coefficients are shown on the graph. (B) Influence of sequencing depth on the estimation of gene expression level and gene detection. Reads for each method were randomly sampled in triplicate. The average of the Pearson correlation coefficients between TruSeq RNA and randomly sampled data for FL-cDNA-Seq and SMART-Seq is shown (left). The average number of genes with an expression level of more than 1 tpm or ppm is shown (right). (C) Comparison to qRT-qPCR. Forty-four genes detected by all methods were analyzed. The gene expression of these genes was normalized to GAPDH. Pearson correlation coefficients are shown on the graph. qRT-qPCR data of LC2/ad was obtained as in our previous study. 16

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Evaluation and application of RNA-Seq by MinION

    doi: 10.1093/dnares/dsy038

    Figure Lengend Snippet: Comparison of FL-cDNA-Seq and Illumina RNA-Seq. (A) The gene expression of FL-cDNA-Seq of LC2/ad (R9.4) was compared with that of TruSeq RNA (left) and SMART-Seq (right). Pearson correlation coefficients are shown on the graph. (B) Influence of sequencing depth on the estimation of gene expression level and gene detection. Reads for each method were randomly sampled in triplicate. The average of the Pearson correlation coefficients between TruSeq RNA and randomly sampled data for FL-cDNA-Seq and SMART-Seq is shown (left). The average number of genes with an expression level of more than 1 tpm or ppm is shown (right). (C) Comparison to qRT-qPCR. Forty-four genes detected by all methods were analyzed. The gene expression of these genes was normalized to GAPDH. Pearson correlation coefficients are shown on the graph. qRT-qPCR data of LC2/ad was obtained as in our previous study. 16

    Article Snippet: Optimization of the cDNA-Seq procedure Using the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio, which is based on the Smart-Seq2 method, we synthesized full-length cDNA (FL-cDNA) from 50 ng total RNA isolated from seven lung adenocarcinoma-derived cell lines: PC-7, PC-9, H1975, H2228, VMRC-LCD, LC2/ad, and A549 ( ).

    Techniques: RNA Sequencing Assay, Expressing, Sequencing, Real-time Polymerase Chain Reaction

    While most protein coding genes are commonly detected, lincRNAs appear more method specific. ( A ) Overlap between protein coding genes detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( B ) Expression counts for protein coding genes detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray). ( C ) Overlap between lncRNAs detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( D ) Expression counts for lncRNAs detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray).

    Journal: Nucleic Acids Research

    Article Title: SMARTer single cell total RNA sequencing

    doi: 10.1093/nar/gkz535

    Figure Lengend Snippet: While most protein coding genes are commonly detected, lincRNAs appear more method specific. ( A ) Overlap between protein coding genes detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( B ) Expression counts for protein coding genes detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray). ( C ) Overlap between lncRNAs detected in polyA[+] (1 million reads) and total RNA (1 million reads) libraries. ( D ) Expression counts for lncRNAs detected in only polyA[+] libraries (red), only total RNA libraries (green) or both (gray).

    Article Snippet: Single cell polyA[+] RNA sequencing on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (SMART-Seq v4, polyA[+] RNA, Takara, 635026) according to manufacturer's instructions.

    Techniques: Expressing

    Read distribution differs between polyA[+] and total RNA libraries. ( A ) Percentage of reads derived from nuclear RNA, mitochondrial RNA and ribosomal RNA per cell quantified with STAR. ( B ) Percentage of the reads originating from nuclear chromosomes derived from exonic, intronic and intergenic regions per cell quantified with STAR. ( C ) Percentage of exonic reads attributed to the different biotypes per cell quantified with Kallisto.

    Journal: Nucleic Acids Research

    Article Title: SMARTer single cell total RNA sequencing

    doi: 10.1093/nar/gkz535

    Figure Lengend Snippet: Read distribution differs between polyA[+] and total RNA libraries. ( A ) Percentage of reads derived from nuclear RNA, mitochondrial RNA and ribosomal RNA per cell quantified with STAR. ( B ) Percentage of the reads originating from nuclear chromosomes derived from exonic, intronic and intergenic regions per cell quantified with STAR. ( C ) Percentage of exonic reads attributed to the different biotypes per cell quantified with Kallisto.

    Article Snippet: Single cell polyA[+] RNA sequencing on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (SMART-Seq v4, polyA[+] RNA, Takara, 635026) according to manufacturer's instructions.

    Techniques: Derivative Assay

    Pathway analysis for polyA[+] RNA and total RNA libraries is similar. ( A ) Gene set enrichment analysis for all hallmark pathways resulted in the same significant ( P adj

    Journal: Nucleic Acids Research

    Article Title: SMARTer single cell total RNA sequencing

    doi: 10.1093/nar/gkz535

    Figure Lengend Snippet: Pathway analysis for polyA[+] RNA and total RNA libraries is similar. ( A ) Gene set enrichment analysis for all hallmark pathways resulted in the same significant ( P adj

    Article Snippet: Single cell polyA[+] RNA sequencing on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (SMART-Seq v4, polyA[+] RNA, Takara, 635026) according to manufacturer's instructions.

    Techniques:

    Mean read distributions are similar for total RNA sequencing libraries generated on C1 or in microplates. ( A ) Mean percentage of reads derived from nuclear RNA, mitochondrial RNA and ribosomal RNA quantified with STAR. Single cell total RNA libraries of the FACS sorted cells were generated using two different reagent kits (#634413, denoted with * and #634444, denoted with °). ( B ) Mean percentage of reads originating from nuclear chromosomes derived from exonic, intronic and intergenic regions quantified with STAR. ( C ) Mean percentage of exonic reads attributed to the different RNA biotypes quantified with Kallisto.

    Journal: Nucleic Acids Research

    Article Title: SMARTer single cell total RNA sequencing

    doi: 10.1093/nar/gkz535

    Figure Lengend Snippet: Mean read distributions are similar for total RNA sequencing libraries generated on C1 or in microplates. ( A ) Mean percentage of reads derived from nuclear RNA, mitochondrial RNA and ribosomal RNA quantified with STAR. Single cell total RNA libraries of the FACS sorted cells were generated using two different reagent kits (#634413, denoted with * and #634444, denoted with °). ( B ) Mean percentage of reads originating from nuclear chromosomes derived from exonic, intronic and intergenic regions quantified with STAR. ( C ) Mean percentage of exonic reads attributed to the different RNA biotypes quantified with Kallisto.

    Article Snippet: Single cell polyA[+] RNA sequencing on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (SMART-Seq v4, polyA[+] RNA, Takara, 635026) according to manufacturer's instructions.

    Techniques: RNA Sequencing Assay, Generated, Derivative Assay, FACS