Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Article Title: Evaluation and application of RNA-Seq by MinION
Figure Lengend Snippet: Comparison of FL-cDNA-Seq and Illumina RNA-Seq. (A) The gene expression of FL-cDNA-Seq of LC2/ad (R9.4) was compared with that of TruSeq RNA (left) and SMART-Seq (right). Pearson correlation coefficients are shown on the graph. (B) Influence of sequencing depth on the estimation of gene expression level and gene detection. Reads for each method were randomly sampled in triplicate. The average of the Pearson correlation coefficients between TruSeq RNA and randomly sampled data for FL-cDNA-Seq and SMART-Seq is shown (left). The average number of genes with an expression level of more than 1 tpm or ppm is shown (right). (C) Comparison to qRT-qPCR. Forty-four genes detected by all methods were analyzed. The gene expression of these genes was normalized to GAPDH. Pearson correlation coefficients are shown on the graph. qRT-qPCR data of LC2/ad was obtained as in our previous study. 16
Article Snippet: Optimization of the cDNA-Seq procedure Using the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio, which is based on the Smart-Seq2 method, we synthesized full-length cDNA (FL-cDNA) from 50 ng total RNA isolated from seven lung adenocarcinoma-derived cell lines: PC-7, PC-9, H1975, H2228, VMRC-LCD, LC2/ad, and A549 ( ).
Techniques: RNA Sequencing Assay, Expressing, Sequencing, Real-time Polymerase Chain Reaction