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  • 99
    New England Biolabs ultra q5 master mix
    Ultra Q5 Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 12388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra q5 master mix/product/New England Biolabs
    Average 99 stars, based on 12388 article reviews
    Price from $9.99 to $1999.99
    ultra q5 master mix - by Bioz Stars, 2020-05
    99/100 stars
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    94
    New England Biolabs nebnext ultra ii q5 master mix
    Evaluation of three different polymerase master mixes in the first and second stages of PCR, for sequencing library preparation. Amplification reaction mixes were assembled with TaqMan genotyping master mix, HotStarTaq Plus master mix, or <t>NEBNext</t> Ultra II Q5 mix during first-stage PCR. All of the first-stage PCR products were assembled in NEBNext Ultra II <t>Q5</t> master mix ( A ), HotStarTaq Plus master mix ( B ), or TaqMan genotyping master mix ( C ) for second-stage PCR. Libraries were purified with solid-phase reversible immobilization beads and analyzed on an Agilent 2100 DNA bioanalyzer. A 300- to 400-bp target-specific library is indicated by a bracket . Note that the fragments of 100 to 200 bp predominantly contained primer dimers. Green and purple bars indicate lower and upper markers, respectively. All samples were evaluated in triplicate.
    Nebnext Ultra Ii Q5 Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii q5 master mix/product/New England Biolabs
    Average 94 stars, based on 101 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii q5 master mix - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext q5 hot start hifi pcr master mix
    Evaluation of three different polymerase master mixes in the first and second stages of PCR, for sequencing library preparation. Amplification reaction mixes were assembled with TaqMan genotyping master mix, HotStarTaq Plus master mix, or <t>NEBNext</t> Ultra II Q5 mix during first-stage PCR. All of the first-stage PCR products were assembled in NEBNext Ultra II <t>Q5</t> master mix ( A ), HotStarTaq Plus master mix ( B ), or TaqMan genotyping master mix ( C ) for second-stage PCR. Libraries were purified with solid-phase reversible immobilization beads and analyzed on an Agilent 2100 DNA bioanalyzer. A 300- to 400-bp target-specific library is indicated by a bracket . Note that the fragments of 100 to 200 bp predominantly contained primer dimers. Green and purple bars indicate lower and upper markers, respectively. All samples were evaluated in triplicate.
    Nebnext Q5 Hot Start Hifi Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext q5 hot start hifi pcr master mix/product/New England Biolabs
    Average 99 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    nebnext q5 hot start hifi pcr master mix - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Evaluation of three different polymerase master mixes in the first and second stages of PCR, for sequencing library preparation. Amplification reaction mixes were assembled with TaqMan genotyping master mix, HotStarTaq Plus master mix, or NEBNext Ultra II Q5 mix during first-stage PCR. All of the first-stage PCR products were assembled in NEBNext Ultra II Q5 master mix ( A ), HotStarTaq Plus master mix ( B ), or TaqMan genotyping master mix ( C ) for second-stage PCR. Libraries were purified with solid-phase reversible immobilization beads and analyzed on an Agilent 2100 DNA bioanalyzer. A 300- to 400-bp target-specific library is indicated by a bracket . Note that the fragments of 100 to 200 bp predominantly contained primer dimers. Green and purple bars indicate lower and upper markers, respectively. All samples were evaluated in triplicate.

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: Rational “Error Elimination” Approach to Evaluating Molecular Barcoded Next-Generation Sequencing Data Identifies Low-Frequency Mutations in Hematologic Malignancies

    doi: 10.1016/j.jmoldx.2019.01.008

    Figure Lengend Snippet: Evaluation of three different polymerase master mixes in the first and second stages of PCR, for sequencing library preparation. Amplification reaction mixes were assembled with TaqMan genotyping master mix, HotStarTaq Plus master mix, or NEBNext Ultra II Q5 mix during first-stage PCR. All of the first-stage PCR products were assembled in NEBNext Ultra II Q5 master mix ( A ), HotStarTaq Plus master mix ( B ), or TaqMan genotyping master mix ( C ) for second-stage PCR. Libraries were purified with solid-phase reversible immobilization beads and analyzed on an Agilent 2100 DNA bioanalyzer. A 300- to 400-bp target-specific library is indicated by a bracket . Note that the fragments of 100 to 200 bp predominantly contained primer dimers. Green and purple bars indicate lower and upper markers, respectively. All samples were evaluated in triplicate.

    Article Snippet: The second stage of PCR was performed in 40 μL volume using 1× NEBNext Ultra II Q5 master mix, HotStarTaq Plus master mix, or 1× TaqMan genotyping master mix; 17 μL of purified product from the first-stage PCR; and 0.5 μmol/L Illumina index primers (San Diego, CA).

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification, Purification