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    • ucp2  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc ucp2
    Primer sequences used to determine adipose tissue gene expression in mice.
    Ucp2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ucp2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ucp2 - by Bioz Stars, 2023-09
    94/100 stars
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    gene exp ucp2 hs00163349 m1  (Thermo Fisher)
    89
    Thermo Fisher gene exp ucp2 hs00163349 m1
    A set of ROS regulating enzymes is coordinately controlled by PPARγ activation. Karpas 299 and SUP-M2 cells treated with 2 μmol/L rosiglitazone or DMSO were harvested at 42 hours after serum withdrawal. A: p67 mRNA was measured by real-time RT-PCR (left). The amount of p67 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. p67 protein levels were determined by Western blot analysis in whole-cell lysates (right). D, DMSO; R, rosiglitazone. B: <t>UCP2</t> mRNA was measured by real-time RT-PCR (left). The amount of UCP2 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. <t>UCP2</t> <t>protein</t> levels were determined by Western blot analysis in whole cell lysates (right). D, DMSO; R, rosiglitazone. C: Mn-SOD mRNA was measured by real-time RT-PCR (left). The amount of Mn-SOD mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Mn-SOD activity was determined in whole-cell lysates. D: CuZn-SOD and catalase mRNA levels were determined by real-time RT-PCR. The amount of mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Data shown in A–D are mean ± SE of at least three independent experiments.
    Gene Exp Ucp2 Hs00163349 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ucp2 hs00163349 m1/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ucp2 hs00163349 m1 - by Bioz Stars, 2023-09
    89/100 stars
    		  Buy from Supplier
    gene exp ucp2 mm00627599 m1  (Thermo Fisher)
    98
    Thermo Fisher gene exp ucp2 mm00627599 m1
    A set of ROS regulating enzymes is coordinately controlled by PPARγ activation. Karpas 299 and SUP-M2 cells treated with 2 μmol/L rosiglitazone or DMSO were harvested at 42 hours after serum withdrawal. A: p67 mRNA was measured by real-time RT-PCR (left). The amount of p67 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. p67 protein levels were determined by Western blot analysis in whole-cell lysates (right). D, DMSO; R, rosiglitazone. B: <t>UCP2</t> mRNA was measured by real-time RT-PCR (left). The amount of UCP2 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. <t>UCP2</t> <t>protein</t> levels were determined by Western blot analysis in whole cell lysates (right). D, DMSO; R, rosiglitazone. C: Mn-SOD mRNA was measured by real-time RT-PCR (left). The amount of Mn-SOD mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Mn-SOD activity was determined in whole-cell lysates. D: CuZn-SOD and catalase mRNA levels were determined by real-time RT-PCR. The amount of mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Data shown in A–D are mean ± SE of at least three independent experiments.
    Gene Exp Ucp2 Mm00627599 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ucp2 mm00627599 m1/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ucp2 mm00627599 m1 - by Bioz Stars, 2023-09
    98/100 stars
    		  Buy from Supplier
    gene exp ucp2 rn01754856 m1  (Thermo Fisher)
    98
    Thermo Fisher gene exp ucp2 rn01754856 m1
    A set of ROS regulating enzymes is coordinately controlled by PPARγ activation. Karpas 299 and SUP-M2 cells treated with 2 μmol/L rosiglitazone or DMSO were harvested at 42 hours after serum withdrawal. A: p67 mRNA was measured by real-time RT-PCR (left). The amount of p67 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. p67 protein levels were determined by Western blot analysis in whole-cell lysates (right). D, DMSO; R, rosiglitazone. B: <t>UCP2</t> mRNA was measured by real-time RT-PCR (left). The amount of UCP2 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. <t>UCP2</t> <t>protein</t> levels were determined by Western blot analysis in whole cell lysates (right). D, DMSO; R, rosiglitazone. C: Mn-SOD mRNA was measured by real-time RT-PCR (left). The amount of Mn-SOD mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Mn-SOD activity was determined in whole-cell lysates. D: CuZn-SOD and catalase mRNA levels were determined by real-time RT-PCR. The amount of mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Data shown in A–D are mean ± SE of at least three independent experiments.
    Gene Exp Ucp2 Rn01754856 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ucp2 rn01754856 m1/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ucp2 rn01754856 m1 - by Bioz Stars, 2023-09
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Primer sequences used to determine adipose tissue gene expression in mice.

    Journal: The Journal of endocrinology

    Article Title: Increased Susceptibility to OVX-Associated Metabolic Dysfunction in UCP1 Null Mice

    doi: 10.1530/JOE-18-0139

    Figure Lengend Snippet: Primer sequences used to determine adipose tissue gene expression in mice.

    Article Snippet: Briefly, protein samples (10 μg/lane were separated by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and probed with primary antibodies, including UCP1 (#U6382, 1:1000; Sigma-Aldrich), UCP2 (#89326, 1:1000; Cell Signaling), Acetyl Co-A Carboxylase or ACC (#3662, 1:1000, Cell Signaling), Phosphorylated Acc or PACC(#3661, 1:1000 Cell Signaling), OXPHOS (#M5604, 1:1000, MitoSciences), Ampk (#2532, 1:1000, Cell Signaling), AMPK-SER485(4185, 1:1000, Cell Signaling), AMPK-THR172 (#2531, 1:1000, Cell Signaling), Estrogen Receptor Alpha (Yasrebi, et al.)(#75635, 1:1000, Abcam), Estrogen Receptor Beta (ERB) (#AB3577, 1:2000, Abcam), Glut4 (#2213, 1:1000, Cell Signaling), Akt (#4691, 1:500, Cell Signaling).

    Techniques: Expressing

    A set of ROS regulating enzymes is coordinately controlled by PPARγ activation. Karpas 299 and SUP-M2 cells treated with 2 μmol/L rosiglitazone or DMSO were harvested at 42 hours after serum withdrawal. A: p67 mRNA was measured by real-time RT-PCR (left). The amount of p67 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. p67 protein levels were determined by Western blot analysis in whole-cell lysates (right). D, DMSO; R, rosiglitazone. B: UCP2 mRNA was measured by real-time RT-PCR (left). The amount of UCP2 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. UCP2 protein levels were determined by Western blot analysis in whole cell lysates (right). D, DMSO; R, rosiglitazone. C: Mn-SOD mRNA was measured by real-time RT-PCR (left). The amount of Mn-SOD mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Mn-SOD activity was determined in whole-cell lysates. D: CuZn-SOD and catalase mRNA levels were determined by real-time RT-PCR. The amount of mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Data shown in A–D are mean ± SE of at least three independent experiments.

    Journal:

    Article Title: Activation of Peroxisome Proliferator-Activated Receptor ? Contributes to the Survival of T Lymphoma Cells by Affecting Cellular Metabolism

    doi: 10.2353/ajpath.2007.060651

    Figure Lengend Snippet: A set of ROS regulating enzymes is coordinately controlled by PPARγ activation. Karpas 299 and SUP-M2 cells treated with 2 μmol/L rosiglitazone or DMSO were harvested at 42 hours after serum withdrawal. A: p67 mRNA was measured by real-time RT-PCR (left). The amount of p67 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. p67 protein levels were determined by Western blot analysis in whole-cell lysates (right). D, DMSO; R, rosiglitazone. B: UCP2 mRNA was measured by real-time RT-PCR (left). The amount of UCP2 mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. UCP2 protein levels were determined by Western blot analysis in whole cell lysates (right). D, DMSO; R, rosiglitazone. C: Mn-SOD mRNA was measured by real-time RT-PCR (left). The amount of Mn-SOD mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Mn-SOD activity was determined in whole-cell lysates. D: CuZn-SOD and catalase mRNA levels were determined by real-time RT-PCR. The amount of mRNA in DMSO-treated Karpas 299 cells was arbitrarily set as 100%. Data shown in A–D are mean ± SE of at least three independent experiments.

    Article Snippet: Primers and probe were purchased from ABI for detection of PPARγ (Hs00234592_m1), catalase (Hs00156308_m1), Cu, Zn superoxide dismutase (CuZn-SOD, Hs00166575_m1), p67 (Hs00166416_m1), and UCP2 (Hs00163349_m1); sequences are not provided by the manufacturer.

    Techniques: Activation Assay, Quantitative RT-PCR, Western Blot, Activity Assay