u73122 plc β inhibitor Search Results


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  • 97
    Millipore phospholipase cβ plcβ inhibitor u73122
    Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific <t>PLCβ</t> inhibitor <t>(U73122)</t> plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.
    Phospholipase Cβ Plcβ Inhibitor U73122, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospholipase cβ plcβ inhibitor u73122/product/Millipore
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    phospholipase cβ plcβ inhibitor u73122 - by Bioz Stars, 2021-01
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    94
    Cayman Chemical phospholipase c β2 plcβ 2 inhibitor u73122
    Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific <t>PLCβ</t> inhibitor <t>(U73122)</t> plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.
    Phospholipase C β2 Plcβ 2 Inhibitor U73122, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospholipase c β2 plcβ 2 inhibitor u73122/product/Cayman Chemical
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    phospholipase c β2 plcβ 2 inhibitor u73122 - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

    92
    U73122 PLC u73122 plc β inhibitor
    Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific <t>PLCβ</t> inhibitor <t>(U73122)</t> plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.
    U73122 Plc β Inhibitor, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u73122 plc β inhibitor/product/U73122 PLC
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    u73122 plc β inhibitor - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    Image Search Results


    Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific PLCβ inhibitor (U73122) plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.

    Journal: Diabetes

    Article Title: Tub Has a Key Role in Insulin and Leptin Signaling and Action In Vivo in Hypothalamic Nuclei

    doi: 10.2337/db11-1388

    Figure Lengend Snippet: Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific PLCβ inhibitor (U73122) plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.

    Article Snippet: The specific phospholipase Cβ (PLCβ) inhibitor U73122 (1 μmol/L; Sigma-Aldrich) was previously injected to block Tub nuclear translocation induced by the hormones.

    Techniques: Translocation Assay, Mouse Assay, Injection