u373 mg human glioblastoma cells Search Results


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    Thermo Fisher u373 mg human glioblastoma multiforme cells
    U373 Mg Human Glioblastoma Multiforme Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore u373 mg
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    Merck & Co u373 mg
    U373 Mg, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pasteur Institute human u373 mg glioblastoma cells
    RT-qPCR Analyses of EGFR mRNA in <t>U373-MG</t> Cells. To measure the expression of EGFR in <t>glioblastoma</t> cells, the <t>U373-MG</t> cells were transfected with negative control (NC) miRNA and miRNA-7 for 24 and 48 h. Relative EGFR mRNA expression was quantified by RT-qPCR using 2 - (∆∆Ct) method and β-actin as an internal control. Data are presented as mean ± SD of three independent experiments. * p < 0.05; # p > 0.05 versus blank control
    Human U373 Mg Glioblastoma Cells, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CEM Corporation u373 mg astrocytoma cells
    RT-qPCR Analyses of EGFR mRNA in <t>U373-MG</t> Cells. To measure the expression of EGFR in <t>glioblastoma</t> cells, the <t>U373-MG</t> cells were transfected with negative control (NC) miRNA and miRNA-7 for 24 and 48 h. Relative EGFR mRNA expression was quantified by RT-qPCR using 2 - (∆∆Ct) method and β-actin as an internal control. Data are presented as mean ± SD of three independent experiments. * p < 0.05; # p > 0.05 versus blank control
    U373 Mg Astrocytoma Cells, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RT-qPCR Analyses of EGFR mRNA in U373-MG Cells. To measure the expression of EGFR in glioblastoma cells, the U373-MG cells were transfected with negative control (NC) miRNA and miRNA-7 for 24 and 48 h. Relative EGFR mRNA expression was quantified by RT-qPCR using 2 - (∆∆Ct) method and β-actin as an internal control. Data are presented as mean ± SD of three independent experiments. * p < 0.05; # p > 0.05 versus blank control

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: RT-qPCR Analyses of EGFR mRNA in U373-MG Cells. To measure the expression of EGFR in glioblastoma cells, the U373-MG cells were transfected with negative control (NC) miRNA and miRNA-7 for 24 and 48 h. Relative EGFR mRNA expression was quantified by RT-qPCR using 2 - (∆∆Ct) method and β-actin as an internal control. Data are presented as mean ± SD of three independent experiments. * p < 0.05; # p > 0.05 versus blank control

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Negative Control

    EGFR Protein Expression Levels in Glioblastoma Cells Transfected with miRNAs. Representative western blots of EGFR and β-actin proteins after 24 (A) and 48 (B). The effect of miRNA-7 on expression levels of EGFR was quantified using densitometry and normalized to the respective β-actin (C). The results are expressed as mean±SD of the results of three independent experiments. * p < 0.05; # p > 0.05 versus blank control

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: EGFR Protein Expression Levels in Glioblastoma Cells Transfected with miRNAs. Representative western blots of EGFR and β-actin proteins after 24 (A) and 48 (B). The effect of miRNA-7 on expression levels of EGFR was quantified using densitometry and normalized to the respective β-actin (C). The results are expressed as mean±SD of the results of three independent experiments. * p < 0.05; # p > 0.05 versus blank control

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: Expressing, Transfection, Western Blot

    Growth Inhibition of U373-MG Cells Transfected with miRNA-7. The cells were transfected with negative control (NC) miRNA and miRNA-7 for 24-120 h. The cell growth rate was analyzed by trypan blue exclusion assay at the end of each period. The results are represented as mean ± SD (n=3). * p < 0.05 versus blank control or NC miRNA

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: Growth Inhibition of U373-MG Cells Transfected with miRNA-7. The cells were transfected with negative control (NC) miRNA and miRNA-7 for 24-120 h. The cell growth rate was analyzed by trypan blue exclusion assay at the end of each period. The results are represented as mean ± SD (n=3). * p < 0.05 versus blank control or NC miRNA

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: Inhibition, Transfection, Negative Control, Trypan Blue Exclusion Assay

    Synergistic Effect between miRNA-7 and Erlotinib in Human U373-MGCells. Twenty-four (A and B) and forty-eight (C and D) hours effect of miRNA-7 on the sensitivity of the glioblastoma cells to erlotinib were measured by MTT assay as described in the method section. The cell survival curves were plotted using GraphPad software. The results are expressed as mean ± SD of three independent experiments. Combination index (CI) versus fractional effect (Fa) was plotted using the Chou-Talalay method and CalcuSyn software. A horizontal dashed line shows CI of 1

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: Synergistic Effect between miRNA-7 and Erlotinib in Human U373-MGCells. Twenty-four (A and B) and forty-eight (C and D) hours effect of miRNA-7 on the sensitivity of the glioblastoma cells to erlotinib were measured by MTT assay as described in the method section. The cell survival curves were plotted using GraphPad software. The results are expressed as mean ± SD of three independent experiments. Combination index (CI) versus fractional effect (Fa) was plotted using the Chou-Talalay method and CalcuSyn software. A horizontal dashed line shows CI of 1

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: MTT Assay, Software

    IC 50 of Erlotinib Alone and in Combination with miRNAs in  U373-MG  Cells, after 24 and 48 h of Treatment

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: IC 50 of Erlotinib Alone and in Combination with miRNAs in U373-MG Cells, after 24 and 48 h of Treatment

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques:

    CI Analysis of Combination Treatment in  U373-MG  Cells

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: CI Analysis of Combination Treatment in U373-MG Cells

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: Concentration Assay

    Effect of miRNA-7 on Erlotinib-Mediated Apoptosis in U373-MG Cells. The cells were treated with miRNA-7 (50 nM), negative control (NC) miRNA (50 nM) and erlotinib (IC50 doses of 24 and 48 h), alone and in combination. After 24 and 48 h, apoptosis was measured by cell death ELISA assay. The data are expressed as mean ± SD (n=3). *p < 0.05 relative to blank control; #p < 0.05 relative to miRNA-7 or erlotinib alone

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: MiRNA-7 Replacement Effect on Proliferation and Tarceva-Sensitivity in U373-MG Cell Line

    doi: 10.31557/APJCP.2020.21.6.1747

    Figure Lengend Snippet: Effect of miRNA-7 on Erlotinib-Mediated Apoptosis in U373-MG Cells. The cells were treated with miRNA-7 (50 nM), negative control (NC) miRNA (50 nM) and erlotinib (IC50 doses of 24 and 48 h), alone and in combination. After 24 and 48 h, apoptosis was measured by cell death ELISA assay. The data are expressed as mean ± SD (n=3). *p < 0.05 relative to blank control; #p < 0.05 relative to miRNA-7 or erlotinib alone

    Article Snippet: Cell culture The human U373-MG glioblastoma cells were purchased from Pasteur Institute (Tehran, Iran).

    Techniques: Negative Control, Enzyme-linked Immunosorbent Assay