u taq dna ligase Search Results


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  • 99
    New England Biolabs u taq dna ligase
    FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified <t>NC-DNA</t> (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and <t>Taq</t> DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P
    U Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher ptz19r dna
    FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified <t>NC-DNA</t> (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and <t>Taq</t> DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P
    Ptz19r Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dog genomic dna
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dog Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATUM template dna
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Template Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 94/100, based on 2248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 3730xl dna analyzer
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dna free kit
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13017 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher dna ligase
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1000 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Qiagen taq dna ligase
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Taq Dna Ligase, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa dna ligation kit
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Ligation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher low dna mass ladder
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Low Dna Mass Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies dna 1000 kit
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna 1000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 3503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs dna ligase
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche taq dna ploymerase
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Taq Dna Ploymerase, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Axygen dna purification kit
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Purification Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 93/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    PerkinElmer dna thermal cycler
    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic <t>DNA</t> is cleaved with the frequently cutting restriction enzyme, <t>NlaIII.</t> ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.
    Dna Thermal Cycler, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 4022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs dna ligase buffer
    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, <t>DNA</t> replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to <t>T4.</t> The genetic map was created using the Geneious software.
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad dna engine thermocycler
    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, <t>DNA</t> replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to <t>T4.</t> The genetic map was created using the Geneious software.
    Dna Engine Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified NC-DNA (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and Taq DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P

    Journal: PLoS Pathogens

    Article Title: Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus

    doi: 10.1371/journal.ppat.1007124

    Figure Lengend Snippet: FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified NC-DNA (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and Taq DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P

    Article Snippet: NC-DNA (108 copies) was incubated with 32 units (U) of Thermostable FEN1 in ThermoPol Buffer (New England Biolabs) at 65°C for 10 min, followed by incubation with 8 U of Bst DNA polymerase, 40 U of Taq DNA ligase, 100 μM dNTPs, and NAD+ (all from New England Biolabs).

    Techniques: In Vitro, Tube Formation Assay, Purification, Incubation, Recombinant, Real-time Polymerase Chain Reaction, Amplification, Plasmid Preparation, Sequencing

    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Journal: Nucleic Acids Research

    Article Title: Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

    doi: 10.1093/nar/gni058

    Figure Lengend Snippet: Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Article Snippet: Synthesis of double-stranded DNA was performed by thermal cycling of 10 μl phosphorylated sense oligonucleotides, 10 μl phosphorylated antisense oligonucleotides, 3 μl 10× Taq DNA ligase buffer (NEB) in a total volume of 30 μl.

    Techniques: Functional Assay, Clone Assay, Activity Assay, Amplification, Incubation

    Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic DNA is cleaved with the frequently cutting restriction enzyme, NlaIII. ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.

    Journal: Genome Research

    Article Title: Short interspersed elements (SINEs) are a major source of canine genomic diversity

    doi: 10.1101/gr.3765505

    Figure Lengend Snippet: Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. ( A ) Genomic DNA is cleaved with the frequently cutting restriction enzyme, NlaIII. ( B ) The cleaved fragments are self-ligated. ( C ) The circularized products are subjected to PCR using SINEC_Cf-specific primers. ( D ) The linear products are size-selected and cloned in a plasmid vector. ( E ) Inserts are sequenced with a vector-specific primer.

    Article Snippet: Dog genomic DNA (100 ng; Novagen) was digested with NlaIII in 20 μL, heat-inactivated (65°C, 20 min), and ligated overnight at 20°C in 500 μL with 10,000 U of DNA ligase (New England Biolabs).

    Techniques: Sequencing, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Journal: Viruses

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    doi: 10.3390/v10040174

    Figure Lengend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Article Snippet: The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% ( w / v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs).

    Techniques: Lysis, Software