type strain pseudomonas tolaasii atcc 33618 Search Results


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    Bruker Corporation kappa apexii area detector diffractometer 33618
    Kappa Apexii Area Detector Diffractometer 33618, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kappa apexii area detector diffractometer 33618/product/Bruker Corporation
    Average 86 stars, based on 1 article reviews
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    Santa Cruz Biotechnology atp synthase β subunit
    ( a ) Synaptic mitochondria from 5xFAD mice demonstrated an age-dependent decrease in their respiratory control ratio (RCR). Six nonTg and 5 5xFAD mice at 4 months old and 6 nonTg and 5 5xFAD mice at 9 months old were used. ( b ) Synaptic mitochondria from 5xFAD mice had an age-dependent decrease in <t>ATP</t> synthesis. Six nonTg and 6 5xFAD mice at 4 months old and those from six nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( c ) Synaptic mitochondria from 5xFAD mice demonstrated an early decrease in the F1FO-ATP <t>synthase</t> catalytic activity at 4 months old which was exacerbated at 9 months old. Five mice of each group at 4 months old and seven nonTg and nine 5xFAD mice at 9 months old were used in the experiment. ( d ) Age-dependent increase in oligomycin-insensitive respiration of synaptic mitochondria from 5xFAD mice. Six nonTg and five 5xFAD mice at 4 months old, and six nonTg and five 5xFAD mice at 9 months old were used in the experiments. ( e , f ) Decreased oligomycin sensitivity of synaptic mitochondria from 5xFAD mice at 4 ( e ) and 9 months old ( f ). All data are presented as percentage of the activity of the corresponding vehicle-treated mitochondrial fractions. Six nonTg and five 5xFAD mice at 4 months old, and seven nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( g , h ) Increased F1 dissociation in synaptic mitochondria from 5xFAD mice. ( g ) The analysis of free F1. ( h ) The left panel is the representative of images collected from seven nonTg and six 5xFAD mice at 4 months old, and six nonTg and six 5xFAD mice at 9 months old. F1 was determined by probing with anti-β subunit antibody and the molecular weight of the bands. The same amount of samples was used for SDS–PAGE and Tom40 and <t>β</t> <t>subunit</t> were detected to show the loading amount of mitochondrial proteins. The right panel is the coomassie blue staining before immunoblotting to indicate the loading amount of samples. Error bars represent s.e.m.
    Atp Synthase β Subunit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp synthase β subunit/product/Santa Cruz Biotechnology
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    86
    Santa Cruz Biotechnology polyclonal antibody
    ( a ) Synaptic mitochondria from 5xFAD mice demonstrated an age-dependent decrease in their respiratory control ratio (RCR). Six nonTg and 5 5xFAD mice at 4 months old and 6 nonTg and 5 5xFAD mice at 9 months old were used. ( b ) Synaptic mitochondria from 5xFAD mice had an age-dependent decrease in <t>ATP</t> synthesis. Six nonTg and 6 5xFAD mice at 4 months old and those from six nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( c ) Synaptic mitochondria from 5xFAD mice demonstrated an early decrease in the F1FO-ATP <t>synthase</t> catalytic activity at 4 months old which was exacerbated at 9 months old. Five mice of each group at 4 months old and seven nonTg and nine 5xFAD mice at 9 months old were used in the experiment. ( d ) Age-dependent increase in oligomycin-insensitive respiration of synaptic mitochondria from 5xFAD mice. Six nonTg and five 5xFAD mice at 4 months old, and six nonTg and five 5xFAD mice at 9 months old were used in the experiments. ( e , f ) Decreased oligomycin sensitivity of synaptic mitochondria from 5xFAD mice at 4 ( e ) and 9 months old ( f ). All data are presented as percentage of the activity of the corresponding vehicle-treated mitochondrial fractions. Six nonTg and five 5xFAD mice at 4 months old, and seven nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( g , h ) Increased F1 dissociation in synaptic mitochondria from 5xFAD mice. ( g ) The analysis of free F1. ( h ) The left panel is the representative of images collected from seven nonTg and six 5xFAD mice at 4 months old, and six nonTg and six 5xFAD mice at 9 months old. F1 was determined by probing with anti-β subunit antibody and the molecular weight of the bands. The same amount of samples was used for SDS–PAGE and Tom40 and <t>β</t> <t>subunit</t> were detected to show the loading amount of mitochondrial proteins. The right panel is the coomassie blue staining before immunoblotting to indicate the loading amount of samples. Error bars represent s.e.m.
    Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Santa Cruz Biotechnology
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    86
    Santa Cruz Biotechnology atp synthase
    ( a ) Synaptic mitochondria from 5xFAD mice demonstrated an age-dependent decrease in their respiratory control ratio (RCR). Six nonTg and 5 5xFAD mice at 4 months old and 6 nonTg and 5 5xFAD mice at 9 months old were used. ( b ) Synaptic mitochondria from 5xFAD mice had an age-dependent decrease in <t>ATP</t> synthesis. Six nonTg and 6 5xFAD mice at 4 months old and those from six nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( c ) Synaptic mitochondria from 5xFAD mice demonstrated an early decrease in the F1FO-ATP <t>synthase</t> catalytic activity at 4 months old which was exacerbated at 9 months old. Five mice of each group at 4 months old and seven nonTg and nine 5xFAD mice at 9 months old were used in the experiment. ( d ) Age-dependent increase in oligomycin-insensitive respiration of synaptic mitochondria from 5xFAD mice. Six nonTg and five 5xFAD mice at 4 months old, and six nonTg and five 5xFAD mice at 9 months old were used in the experiments. ( e , f ) Decreased oligomycin sensitivity of synaptic mitochondria from 5xFAD mice at 4 ( e ) and 9 months old ( f ). All data are presented as percentage of the activity of the corresponding vehicle-treated mitochondrial fractions. Six nonTg and five 5xFAD mice at 4 months old, and seven nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( g , h ) Increased F1 dissociation in synaptic mitochondria from 5xFAD mice. ( g ) The analysis of free F1. ( h ) The left panel is the representative of images collected from seven nonTg and six 5xFAD mice at 4 months old, and six nonTg and six 5xFAD mice at 9 months old. F1 was determined by probing with anti-β subunit antibody and the molecular weight of the bands. The same amount of samples was used for SDS–PAGE and Tom40 and <t>β</t> <t>subunit</t> were detected to show the loading amount of mitochondrial proteins. The right panel is the coomassie blue staining before immunoblotting to indicate the loading amount of samples. Error bars represent s.e.m.
    Atp Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp synthase/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    86
    Santa Cruz Biotechnology anti β atpase antibody
    Expression of the beta chain of ATP synthase <t>(β-ATPase)</t> in the fat body of P. megistus fifth-instar nymphs. Left panel: Fat bodies from insects at 7 d post-ecdysis (fasting condition, PE) and at 7 d after a bloodmeal (fed condition, PBM) were dissected out, their RNA extracted and used in qPCR assays employing specific primers. Results are expressed as relative expression by 1,000 copies of 18S ribosomal RNA ± SEM. ns, no statistically significant differences ( P = 0.095, t = 1.978, df = 6, n = 4, unpaired t -test). Right panel: Homogenates of fat bodies from insects at fasting and fed conditions were subjected to subcellular fractionation as described in the text. The resulting fractions were probed with the anti-β-ATPase antibody and visualized by western blot. Fifty micrograms was loaded in each lane and a homogenate of rat brain was employed as positive control. Similar results were obtained from three separate experiments.
    Anti β Atpase Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti β atpase antibody/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti β atpase antibody - by Bioz Stars, 2024-05
    86/100 stars
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    Image Search Results


    ( a ) Synaptic mitochondria from 5xFAD mice demonstrated an age-dependent decrease in their respiratory control ratio (RCR). Six nonTg and 5 5xFAD mice at 4 months old and 6 nonTg and 5 5xFAD mice at 9 months old were used. ( b ) Synaptic mitochondria from 5xFAD mice had an age-dependent decrease in ATP synthesis. Six nonTg and 6 5xFAD mice at 4 months old and those from six nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( c ) Synaptic mitochondria from 5xFAD mice demonstrated an early decrease in the F1FO-ATP synthase catalytic activity at 4 months old which was exacerbated at 9 months old. Five mice of each group at 4 months old and seven nonTg and nine 5xFAD mice at 9 months old were used in the experiment. ( d ) Age-dependent increase in oligomycin-insensitive respiration of synaptic mitochondria from 5xFAD mice. Six nonTg and five 5xFAD mice at 4 months old, and six nonTg and five 5xFAD mice at 9 months old were used in the experiments. ( e , f ) Decreased oligomycin sensitivity of synaptic mitochondria from 5xFAD mice at 4 ( e ) and 9 months old ( f ). All data are presented as percentage of the activity of the corresponding vehicle-treated mitochondrial fractions. Six nonTg and five 5xFAD mice at 4 months old, and seven nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( g , h ) Increased F1 dissociation in synaptic mitochondria from 5xFAD mice. ( g ) The analysis of free F1. ( h ) The left panel is the representative of images collected from seven nonTg and six 5xFAD mice at 4 months old, and six nonTg and six 5xFAD mice at 9 months old. F1 was determined by probing with anti-β subunit antibody and the molecular weight of the bands. The same amount of samples was used for SDS–PAGE and Tom40 and β subunit were detected to show the loading amount of mitochondrial proteins. The right panel is the coomassie blue staining before immunoblotting to indicate the loading amount of samples. Error bars represent s.e.m.

    Journal: Nature Communications

    Article Title: Deregulation of mitochondrial F1FO-ATP synthase via OSCP in Alzheimer’s disease

    doi: 10.1038/ncomms11483

    Figure Lengend Snippet: ( a ) Synaptic mitochondria from 5xFAD mice demonstrated an age-dependent decrease in their respiratory control ratio (RCR). Six nonTg and 5 5xFAD mice at 4 months old and 6 nonTg and 5 5xFAD mice at 9 months old were used. ( b ) Synaptic mitochondria from 5xFAD mice had an age-dependent decrease in ATP synthesis. Six nonTg and 6 5xFAD mice at 4 months old and those from six nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( c ) Synaptic mitochondria from 5xFAD mice demonstrated an early decrease in the F1FO-ATP synthase catalytic activity at 4 months old which was exacerbated at 9 months old. Five mice of each group at 4 months old and seven nonTg and nine 5xFAD mice at 9 months old were used in the experiment. ( d ) Age-dependent increase in oligomycin-insensitive respiration of synaptic mitochondria from 5xFAD mice. Six nonTg and five 5xFAD mice at 4 months old, and six nonTg and five 5xFAD mice at 9 months old were used in the experiments. ( e , f ) Decreased oligomycin sensitivity of synaptic mitochondria from 5xFAD mice at 4 ( e ) and 9 months old ( f ). All data are presented as percentage of the activity of the corresponding vehicle-treated mitochondrial fractions. Six nonTg and five 5xFAD mice at 4 months old, and seven nonTg and seven 5xFAD mice at 9 months old were used in the experiments. ( g , h ) Increased F1 dissociation in synaptic mitochondria from 5xFAD mice. ( g ) The analysis of free F1. ( h ) The left panel is the representative of images collected from seven nonTg and six 5xFAD mice at 4 months old, and six nonTg and six 5xFAD mice at 9 months old. F1 was determined by probing with anti-β subunit antibody and the molecular weight of the bands. The same amount of samples was used for SDS–PAGE and Tom40 and β subunit were detected to show the loading amount of mitochondrial proteins. The right panel is the coomassie blue staining before immunoblotting to indicate the loading amount of samples. Error bars represent s.e.m.

    Article Snippet: Immunoblot was performed against ATP synthase β subunit (Santa Cruz, #sc-33618, 1:5,000) or F1 (Abcam, #ab109867, 1:2,000) and OSCP (Santa Cruz, #sc-365162, 1:6,000).

    Techniques: Activity Assay, Molecular Weight, SDS Page, Staining, Western Blot

    Expression of the beta chain of ATP synthase (β-ATPase) in the fat body of P. megistus fifth-instar nymphs. Left panel: Fat bodies from insects at 7 d post-ecdysis (fasting condition, PE) and at 7 d after a bloodmeal (fed condition, PBM) were dissected out, their RNA extracted and used in qPCR assays employing specific primers. Results are expressed as relative expression by 1,000 copies of 18S ribosomal RNA ± SEM. ns, no statistically significant differences ( P = 0.095, t = 1.978, df = 6, n = 4, unpaired t -test). Right panel: Homogenates of fat bodies from insects at fasting and fed conditions were subjected to subcellular fractionation as described in the text. The resulting fractions were probed with the anti-β-ATPase antibody and visualized by western blot. Fifty micrograms was loaded in each lane and a homogenate of rat brain was employed as positive control. Similar results were obtained from three separate experiments.

    Journal: Journal of Insect Science

    Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

    doi: 10.1093/jisesa/iez078

    Figure Lengend Snippet: Expression of the beta chain of ATP synthase (β-ATPase) in the fat body of P. megistus fifth-instar nymphs. Left panel: Fat bodies from insects at 7 d post-ecdysis (fasting condition, PE) and at 7 d after a bloodmeal (fed condition, PBM) were dissected out, their RNA extracted and used in qPCR assays employing specific primers. Results are expressed as relative expression by 1,000 copies of 18S ribosomal RNA ± SEM. ns, no statistically significant differences ( P = 0.095, t = 1.978, df = 6, n = 4, unpaired t -test). Right panel: Homogenates of fat bodies from insects at fasting and fed conditions were subjected to subcellular fractionation as described in the text. The resulting fractions were probed with the anti-β-ATPase antibody and visualized by western blot. Fifty micrograms was loaded in each lane and a homogenate of rat brain was employed as positive control. Similar results were obtained from three separate experiments.

    Article Snippet: To analyze the histological distribution of β-ATPase and lipophorin, cryosections were sequentially incubated with an anti-β-ATPase antibody (dilution 1:100, rabbit anti-ATP5B/β-chain of ATP synthase of human origin, sc-33618, Santa Cruz Biotechnology, Inc., Santa Cruz, CA) followed by a goat anti-rabbit IgG labeled with Alexa 568 antibody (dilution 1:400, Molecular Probes, Eugene, OR) and a polyclonal anti-lipophorin-FITC antibody (dilution 1:40) obtained in our laboratory ( ).

    Techniques: Expressing, Fractionation, Western Blot, Positive Control

    Localization of lipophorin (Lp; green) and β-ATPase (red) in the fat body by immunofluorescence. Merge: image showing the partial colocalization of Lp and β-ATPase (arrowheads). The insets show the corresponding DIC images. H, hemolymph. Similar results were obtained from three separate experiments. Bars: 10 µm.

    Journal: Journal of Insect Science

    Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

    doi: 10.1093/jisesa/iez078

    Figure Lengend Snippet: Localization of lipophorin (Lp; green) and β-ATPase (red) in the fat body by immunofluorescence. Merge: image showing the partial colocalization of Lp and β-ATPase (arrowheads). The insets show the corresponding DIC images. H, hemolymph. Similar results were obtained from three separate experiments. Bars: 10 µm.

    Article Snippet: To analyze the histological distribution of β-ATPase and lipophorin, cryosections were sequentially incubated with an anti-β-ATPase antibody (dilution 1:100, rabbit anti-ATP5B/β-chain of ATP synthase of human origin, sc-33618, Santa Cruz Biotechnology, Inc., Santa Cruz, CA) followed by a goat anti-rabbit IgG labeled with Alexa 568 antibody (dilution 1:400, Molecular Probes, Eugene, OR) and a polyclonal anti-lipophorin-FITC antibody (dilution 1:40) obtained in our laboratory ( ).

    Techniques: Immunofluorescence

    The role of the β-chain of ATP synthase (β-ATPase) on lipophorin (Lp)-mediated lipid transfer in the fat body of Panstrongylus megistus fifth-instar nymphs. (A) The fate of the injected anti-β-ATPase antibody in the fat body. The insects were injected with 5 µl (10 µg) of the anti-β-ATPase antibody, and the organs were dissected 1 h post-injection and processed for cryostat sectioning. Tissue sections were incubated with the anti-rabbit IgG antibody labeled with Alexa 568 to visualize the localization of the injected primary antibody. (B) The effect of β-ATPase blocking on Lp binding to the fat body. Insects injected with either an irrelevant antibody (anti-BSA, control) or anti-β-ATPase antibody were subsequently injected with Lp-DiI to trace the lipophorin particle. One hour later, fat bodies were fixed and processed for confocal laser microscopy. (C) The effect of β-ATPase blocking on Lp lipid transfer to the fat body. Insects injected with an irrelevant antibody (anti-BSA, control) or anti-β-ATPase antibody were subsequently injected with Lp-Bodipy-FA to trace the fate of lipophorin lipid cargo. One hour later, fat bodies were fixed and processed for confocal laser microscopy. For (A)–(C), similar results were obtained in three separate experiments. (D) Bodipy-FA fluorescence quantification in total lipid extracts from fat bodies of insects treated as in (C). The fluorescence was normalized by the weight of the tissue ( n = 5). Results were expressed as arbitrary units of fluorescence per milligram of tissue ± SEM. * P = 0.033 ( F = 2.572, df = 8, Unpaired t -test). The insets show the corresponding DIC images. H, hemolymph. Bars: 10 µm.

    Journal: Journal of Insect Science

    Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

    doi: 10.1093/jisesa/iez078

    Figure Lengend Snippet: The role of the β-chain of ATP synthase (β-ATPase) on lipophorin (Lp)-mediated lipid transfer in the fat body of Panstrongylus megistus fifth-instar nymphs. (A) The fate of the injected anti-β-ATPase antibody in the fat body. The insects were injected with 5 µl (10 µg) of the anti-β-ATPase antibody, and the organs were dissected 1 h post-injection and processed for cryostat sectioning. Tissue sections were incubated with the anti-rabbit IgG antibody labeled with Alexa 568 to visualize the localization of the injected primary antibody. (B) The effect of β-ATPase blocking on Lp binding to the fat body. Insects injected with either an irrelevant antibody (anti-BSA, control) or anti-β-ATPase antibody were subsequently injected with Lp-DiI to trace the lipophorin particle. One hour later, fat bodies were fixed and processed for confocal laser microscopy. (C) The effect of β-ATPase blocking on Lp lipid transfer to the fat body. Insects injected with an irrelevant antibody (anti-BSA, control) or anti-β-ATPase antibody were subsequently injected with Lp-Bodipy-FA to trace the fate of lipophorin lipid cargo. One hour later, fat bodies were fixed and processed for confocal laser microscopy. For (A)–(C), similar results were obtained in three separate experiments. (D) Bodipy-FA fluorescence quantification in total lipid extracts from fat bodies of insects treated as in (C). The fluorescence was normalized by the weight of the tissue ( n = 5). Results were expressed as arbitrary units of fluorescence per milligram of tissue ± SEM. * P = 0.033 ( F = 2.572, df = 8, Unpaired t -test). The insets show the corresponding DIC images. H, hemolymph. Bars: 10 µm.

    Article Snippet: To analyze the histological distribution of β-ATPase and lipophorin, cryosections were sequentially incubated with an anti-β-ATPase antibody (dilution 1:100, rabbit anti-ATP5B/β-chain of ATP synthase of human origin, sc-33618, Santa Cruz Biotechnology, Inc., Santa Cruz, CA) followed by a goat anti-rabbit IgG labeled with Alexa 568 antibody (dilution 1:400, Molecular Probes, Eugene, OR) and a polyclonal anti-lipophorin-FITC antibody (dilution 1:40) obtained in our laboratory ( ).

    Techniques: Injection, Incubation, Labeling, Blocking Assay, Binding Assay, Microscopy, Fluorescence