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    • e coli o157 h7 strain edl 933 genome  (Biotechnology Information)
    86
    Biotechnology Information e coli o157 h7 strain edl 933 genome
    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in <t>E.</t> <t>coli.</t> (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.
    E Coli O157 H7 Strain Edl 933 Genome, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ehec o157 h7 strain edl 933  (ATCC)
    86
    ATCC ehec o157 h7 strain edl 933
    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in <t>E.</t> <t>coli.</t> (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.
    Ehec O157 H7 Strain Edl 933, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    edl 933 reference strain atcc 43895  (ATCC)
    86
    ATCC edl 933 reference strain atcc 43895
    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in <t>E.</t> <t>coli.</t> (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.
    Edl 933 Reference Strain Atcc 43895, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference dna  (Thermo Fisher)
    86
    Thermo Fisher reference dna
    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in <t>E.</t> <t>coli.</t> (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.
    Reference Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli strains  (ATCC)
    86
    ATCC e coli strains
    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in <t>E.</t> <t>coli.</t> (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.
    E Coli Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains/product/ATCC
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    e coli strains - by Bioz Stars, 2023-12
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    Image Search Results


    Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in E. coli. (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: l -Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens

    doi: 10.1073/pnas.1919683117

    Figure Lengend Snippet: Arginine transporter ATP-binding protein artP regulates EHEC virulence in vitro. (A) Schematic of the arginine transmembrane transporter systems in E. coli. (B) qRT-PCR analysis to compare the expression of select virulence genes and (C) Western blot analysis of secreted proteins EspB and EspA from WT, ΔartP, and cpl EHEC (complemented with artP gene in pACYC184 vector). Experiments in B and C were performed in microaerophilic conditions using low-glucose DMEM with arginine, and samples were harvested in late log phase. (D) For fluorescein actin staining analysis, HeLa cells were infected with WT, ΔartP and complemented artP EHEC (clp) and incubated in low-glucose DMEM for 4 h postinfection in the presence of arginine. Then, the cells were stained with FITC-phalloidin to visualize actin (green) and propidium iodide to stain for bacteria and nuclei (red). Pedestals were visualized as green puncta. Pedestals were enumerated for each field (60×). (E) The percentage of infected cells and the number of pedestals per infected cell were quantified (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.

    Article Snippet: Reads were mapped to the E. coli O157:H7 strain EDL 933 genome (National Center for Biotechnology Information {"type":"entrez-nucleotide","attrs":{"text":"NZ_CP008957.1","term_id":"749302083","term_text":"NZ_CP008957.1"}} NZ_CP008957.1 ).

    Techniques: Binding Assay, In Vitro, Quantitative RT-PCR, Expressing, Western Blot, Plasmid Preparation, Staining, Infection, Incubation