Journal: Frontiers in Immunology
Article Title: Magnolol Alleviates Inflammatory Responses and Lipid Accumulation by AMP-Activated Protein Kinase-Dependent Peroxisome Proliferator-Activated Receptor α Activation
Figure Lengend Snippet: Effect of magnolol (MG) treatment on AMPK, peroxisome proliferator-activated receptor α (PPARα), AKT, and sterol regulatory element-binding protein 1c (SREBP-1c) in tyloxapol (Ty)-induced hyperlipidemia mice. Mice were administered MG (10 and 20 mg/kg) for 1 h. Afterward, the mice were injected with Ty (500 mg/kg) for 12 h. Liver tissues were gathered and analyzed by western blot. (A) Effects of MG on P-acetyl-CoA carboxylase (ACC), ACC, P-AMPKα, AMPKα, P-AKT, AKT, PPARα, SREBP-1c, P-P65, P65, and TLR4 were measured by western blot analysis. (B–H) Quantification of relative protein expression was performed by software analysis. All data are expressed as the mean ± SD of three independent experiments. Statistically significant at # p
Article Snippet: Dimethylsulfoxide (DMSO), oleic acid (OA), tyloxapol (Ty), and Oil Red O were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Techniques: Binding Assay, Mouse Assay, Injection, Western Blot, Expressing, Software