tween-20 Millipore Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore squalene
    Squalene, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/squalene/product/Millipore
    Average 95 stars, based on 203 article reviews
    Price from $9.99 to $1999.99
    squalene - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher tween 20 pbs
    Tween 20 Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbs/product/Thermo Fisher
    Average 90 stars, based on 725 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbs - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    99
    Millipore tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3080 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20/product/Millipore
    Average 99 stars, based on 3080 article reviews
    Price from $9.99 to $1999.99
    tween 20 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher tween 20 pbst
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbst/product/Thermo Fisher
    Average 99 stars, based on 1235 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbst - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    96
    Millipore 4xssc tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    4xssc Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4xssc tween 20/product/Millipore
    Average 96 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    4xssc tween 20 - by Bioz Stars, 2020-02
    96/100 stars
      Buy from Supplier

    75
    Millipore saline tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Saline Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/saline tween 20/product/Millipore
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    saline tween 20 - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    97
    Merck KGaA tween 20 tbs t
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Tbs T, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 tbs t/product/Merck KGaA
    Average 97 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    tween 20 tbs t - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    99
    Millipore tween 20 detergent tbst buffer
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Detergent Tbst Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 detergent tbst buffer/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    tween 20 detergent tbst buffer - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    79
    Millipore bsa 0 05 tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Bsa 0 05 Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa 0 05 tween 20/product/Millipore
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bsa 0 05 tween 20 - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Millipore tween 20 pbt ph 7 0
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Pbt Ph 7 0, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbt ph 7 0/product/Millipore
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbt ph 7 0 - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Millipore tween 20 actin ac 40
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Actin Ac 40, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 actin ac 40/product/Millipore
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tween 20 actin ac 40 - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    99
    Millipore pbs tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Pbs Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs tween 20/product/Millipore
    Average 99 stars, based on 1548 article reviews
    Price from $9.99 to $1999.99
    pbs tween 20 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc tween 20 pbs
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tween 20 Pbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbs/product/Cell Signaling Technology Inc
    Average 99 stars, based on 138 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbs - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    83
    Millipore tbs kpl gaithersburg md tween 20
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tbs Kpl Gaithersburg Md Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbs kpl gaithersburg md tween 20/product/Millipore
    Average 83 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    tbs kpl gaithersburg md tween 20 - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    90
    Thermo Fisher tbst
    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), <t>T20</t> (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.
    Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 29959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Thermo Fisher
    Average 90 stars, based on 29959 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    95
    Millipore tween80
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween80, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween80/product/Millipore
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    tween80 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    99
    Millipore 1x tris buffered saline tween 20 tbst
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    1x Tris Buffered Saline Tween 20 Tbst, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x tris buffered saline tween 20 tbst/product/Millipore
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    1x tris buffered saline tween 20 tbst - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    81
    Millipore tween 20 passivated microcon ym 3 microfilter
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 20 Passivated Microcon Ym 3 Microfilter, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 passivated microcon ym 3 microfilter/product/Millipore
    Average 81 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    tween 20 passivated microcon ym 3 microfilter - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    99
    Millipore 1x phosphate buffered saline tween 20 solution
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    1x Phosphate Buffered Saline Tween 20 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x phosphate buffered saline tween 20 solution/product/Millipore
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    1x phosphate buffered saline tween 20 solution - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    83
    Millipore tris buffer saline tween 20
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tris Buffer Saline Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffer saline tween 20/product/Millipore
    Average 83 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    tris buffer saline tween 20 - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    86
    Millipore tween 20 mabt 0 9 m maleic acid
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 20 Mabt 0 9 M Maleic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 mabt 0 9 m maleic acid/product/Millipore
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    tween 20 mabt 0 9 m maleic acid - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    97
    Millipore tween 20 tbst buffer buffer 10 mm tris base
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 20 Tbst Buffer Buffer 10 Mm Tris Base, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 tbst buffer buffer 10 mm tris base/product/Millipore
    Average 97 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tween 20 tbst buffer buffer 10 mm tris base - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    80
    Millipore tween 20 phosphate buffer saline tpbs
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 20 Phosphate Buffer Saline Tpbs, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 phosphate buffer saline tpbs/product/Millipore
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tween 20 phosphate buffer saline tpbs - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    92
    Millipore tween 60 p1629
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 60 P1629, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 60 p1629/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tween 60 p1629 - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    77
    Millipore tween 20 sigma st louis mo sterile saline
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tween 20 Sigma St Louis Mo Sterile Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 sigma st louis mo sterile saline/product/Millipore
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tween 20 sigma st louis mo sterile saline - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Millipore tris buffered saline tbs triton x 100 tween 20 urea
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tris Buffered Saline Tbs Triton X 100 Tween 20 Urea, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffered saline tbs triton x 100 tween 20 urea/product/Millipore
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline tbs triton x 100 tween 20 urea - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    Millipore tris buffered saline tbs
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Tris Buffered Saline Tbs, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3847 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffered saline tbs/product/Millipore
    Average 90 stars, based on 3847 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline tbs - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    84
    Millipore 7h9 gc tween 80
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    7h9 Gc Tween 80, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 gc tween 80/product/Millipore
    Average 84 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    7h9 gc tween 80 - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    95
    Millipore phosphate buffer saline tween 20 pbst
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Phosphate Buffer Saline Tween 20 Pbst, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffer saline tween 20 pbst/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer saline tween 20 pbst - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    92
    Millipore polyoxyethylenesorbitan trioleate
    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . <t>Tween80</t> was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P
    Polyoxyethylenesorbitan Trioleate, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyoxyethylenesorbitan trioleate/product/Millipore
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    polyoxyethylenesorbitan trioleate - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    Image Search Results


    HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), T20 (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.

    Journal: Cell Reports

    Article Title: Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State

    doi: 10.1016/j.celrep.2016.12.032

    Figure Lengend Snippet: HIV-1 Fusion Kinetics Is Dynamin-2 Dependent in Both CD4 T Cells and TZM-bl Cells (A) Cartoon depicting the BlaM assay. Upon virion fusion and capsid release, the Vpr-BlaM chimera recognizes a FRET reporter (CCF2) that changes color (green to blue) upon cleavage. (B) Real-time BlaM was applied using HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with HXB2 Env on primary CD4 T cells at different concentrations of dynasore: 0 μM (green dots), 5 μM (black dots), 20 μM (gray dots), and 80 μM (white dots). The proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (C) HIV-1/Vpr-β-Lactamase virions pseudotyped with VSVG turned out not to be fusogenic (red dots) showing the same behavior as HIV1/Vpr-β-Lactamase bald particles (without Env, black dots). (D) HIV-1 virions packaging the Vpr-β-Lactamase chimera and pseudotyped with either VSV-G (cyan dots) or JR-FL (orange dots) were exposed to TZM-bl cells with different concentrations of dynasore (0, 100, 180, 260, 340, and 400 μM) and endpoint BlaM (as defined in Experimental Procedures ) was applied. Higher concentrations of dynasore were required to fully inhibit HIV JRFL (240 μM) as compared with HIV VSVG (180 μM). (E) Time-of-addition BlaM kinetics without spinoculation protocols on HIV VSV-G virions using three different blocks: 400 μM dynasore (open red dots), temperature block (pink crosses), and NH 4 Cl (open green dots). All of the kinetics turned out to be very similar. The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). (F) Time-of-addition BlaM kinetics without spinoculation protocols on HIV JRFL virions using four different blocks: TAK 779 (open blue dots), dynasore (open red dots), T20 (open black dots), and temperature block (pink crosses). The normalized proportion of fusion positive cells versus total number of cells is shown (y axis) versus time, in min (x axis). In all cases, the error bars represent the SD calculated from three independent experiments.

    Article Snippet: The concentration of T20 (Sigma) used to inhibit cell-cell fusion was 40 μg/mL.

    Techniques: Blocking Assay

    In vitro  release of plumbagin from oleic acid-based nanoemulsion (polysorbate 80 (3.5%) as a function of time. SGF, simulated gastric fluid (SGF), and simulated intestinal fluid (SIF) at 37°C was used as release media.

    Journal: BioMed Research International

    Article Title: Plumbagin-Loaded Nanoemulsion Drug Delivery Formulation and Evaluation of Antiproliferative Effect on Prostate Cancer Cells

    doi: 10.1155/2018/9035452

    Figure Lengend Snippet: In vitro release of plumbagin from oleic acid-based nanoemulsion (polysorbate 80 (3.5%) as a function of time. SGF, simulated gastric fluid (SGF), and simulated intestinal fluid (SIF) at 37°C was used as release media.

    Article Snippet: Plumbagin, polysorbate 80, oleic acid and all other chemicals were supplied by Sigma (St. Louis, MO, USA) unless otherwise stated.

    Techniques: In Vitro

    Binding of apoE4 to previously “blocked” ELISA plates. Recombinant apoE4 (dark gray bars), plasma from an APOE ε3/ε4 donor (light grey bars) or PBS (open bars) was allowed to interact with ELISA plate wells previously treated with different suitable blocking solutions: PBS (PBS with no blocking solution), BSA (0.25% BSA solution in 15 mM borate buffer containing 100 mM NaCl, pH 8.5), Superblock (Superblock T20 (ThermoFisher Scientific)), Polysorbate 20 (1% polysorbate 20 in PBS), Triton X-100 (1% Triton X-100 in PBS), Skim milk (5% skim milk in PBS containing 0.1% polysorbate 20), ODGP (8 mM Octyl b-D-glucopyranoside in PBS. Error bars represent the standard deviation of duplicated measures performed in two independent experiments.

    Journal: Scientific Reports

    Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification

    doi: 10.1038/s41598-018-24320-3

    Figure Lengend Snippet: Binding of apoE4 to previously “blocked” ELISA plates. Recombinant apoE4 (dark gray bars), plasma from an APOE ε3/ε4 donor (light grey bars) or PBS (open bars) was allowed to interact with ELISA plate wells previously treated with different suitable blocking solutions: PBS (PBS with no blocking solution), BSA (0.25% BSA solution in 15 mM borate buffer containing 100 mM NaCl, pH 8.5), Superblock (Superblock T20 (ThermoFisher Scientific)), Polysorbate 20 (1% polysorbate 20 in PBS), Triton X-100 (1% Triton X-100 in PBS), Skim milk (5% skim milk in PBS containing 0.1% polysorbate 20), ODGP (8 mM Octyl b-D-glucopyranoside in PBS. Error bars represent the standard deviation of duplicated measures performed in two independent experiments.

    Article Snippet: In brief, 200 microliters of blocking solutions were placed per well: (a) PBS (no blocking solution), (b) BSA-based blocking solution: 0.25% BSA solution in 15 mM borate buffer containing 0.05% polysorbate 20 and 100 mM NaCl, pH 8.5, (c) Superblock (TBS) (Thermo Fisher Scientific #37515) containing 0.1% polysorbate 20 (Superblock-T), (d) 1% polysorbate 20 in PBS, (e) 1%Triton X-100 in PBS, (f) 5% skim milk in PBS containing 0,1% polysorbate 20 and (g) 8 nM Octyl b-D-glucopyranoside (Sigma-Aldrich #O8001) in PBS containing 1.17% BSA.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Blocking Assay, Standard Deviation

    Stability of the binding of apoE to polystyrene as a function of pH, salt and detergent concentrations. Analysis of the stability of the binding of plasma apoE to polystyrene surfaces was challenged by varying pH ( A ), increasing salt concentrations ( B ) or detergent (0–0.5% polysorbate 20 or Triton X-100) ( C ) and detected by polyclonal pan-apoE antibody. Light and dark gray bars represent samples analyzed in wells blocked with a BSA-based or Superblock blocking solutions, respectively. Error bars represent the standard deviation of duplicated measures.

    Journal: Scientific Reports

    Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification

    doi: 10.1038/s41598-018-24320-3

    Figure Lengend Snippet: Stability of the binding of apoE to polystyrene as a function of pH, salt and detergent concentrations. Analysis of the stability of the binding of plasma apoE to polystyrene surfaces was challenged by varying pH ( A ), increasing salt concentrations ( B ) or detergent (0–0.5% polysorbate 20 or Triton X-100) ( C ) and detected by polyclonal pan-apoE antibody. Light and dark gray bars represent samples analyzed in wells blocked with a BSA-based or Superblock blocking solutions, respectively. Error bars represent the standard deviation of duplicated measures.

    Article Snippet: In brief, 200 microliters of blocking solutions were placed per well: (a) PBS (no blocking solution), (b) BSA-based blocking solution: 0.25% BSA solution in 15 mM borate buffer containing 0.05% polysorbate 20 and 100 mM NaCl, pH 8.5, (c) Superblock (TBS) (Thermo Fisher Scientific #37515) containing 0.1% polysorbate 20 (Superblock-T), (d) 1% polysorbate 20 in PBS, (e) 1%Triton X-100 in PBS, (f) 5% skim milk in PBS containing 0,1% polysorbate 20 and (g) 8 nM Octyl b-D-glucopyranoside (Sigma-Aldrich #O8001) in PBS containing 1.17% BSA.

    Techniques: Binding Assay, Blocking Assay, Standard Deviation

    Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . Tween80 was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P

    Journal: Microbiology

    Article Title: Herbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responses

    doi: 10.1099/mic.0.000573

    Figure Lengend Snippet: Change in EOP (a) when S. enterica is (orange) and is not (blue) exposed to co-formulant. Concentration of co-formulant needed to induce a response (b). (a) The x-axis scale is antibiotic concentrations in µg ml −1 . Tween80 was used at 2 % (v/v), CMC was used at 1 % (w/v). (b) The x-axis scale is concentration of surfactants in %. S. enterica : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/4.4/0.03/12/2 µg ml −1 with Tween80 and 1.5/na/na/0.25/2.5 µg ml −1 with CMC. E. coli : concentrations of Amp/Cam/Cip/Kan/Tet, respectively, were na/7.5/0.01/0.5/na μg ml –1 with Tween80 and 5/na/na/10/na μg ml −1 with CMC. Values are averages of at least three independent experiments; error bars are sem (standard deviation/√n). Asterisks indicate P -values (see Methods). * P

    Article Snippet: Co-formulants were Tween80 (BDH, Auckland, NZ), Pulse Penetrant (Yates, Auckland, NZ) containing 800 g l−1 organo-modified polydimethyl siloxane, and carboxymethyl cellulose (Sigma, Auckland, NZ).

    Techniques: Concentration Assay, Chick Chorioallantoic Membrane Assay, Standard Deviation